Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA
Identifieur interne : 000913 ( Pmc/Checkpoint ); précédent : 000912; suivant : 000914Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA
Auteurs : Shuetsu Fukushi [Japon] ; Aiko Fukuma [Japon] ; Takeshi Kurosu [Japon] ; Shumpei Watanabe [Japon] ; Masayuki Shimojima [Japon] ; Kazuya Shirato [Japon] ; Naoko Iwata-Yoshikawa [Japon] ; Noriyo Nagata [Japon] ; Kazuo Ohnishi [Japon] ; Manabu Ato [Japon] ; Simenew Keskes Melaku [Éthiopie] ; Hiroshi Sentsui [Japon] ; Masayuki Saijo [Japon]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2017.
Abstract
Novel monoclonal antibodies against MERS-CoV were produced and characterized. Anti-MERS-CoV antibody detection system by competitive ELISA was developed. The competitive ELISA was validated using sera taken from dromedary camels.
Url:
DOI: 10.1016/j.jviromet.2017.10.008
PubMed: 28993122
PubMed Central: 7113858
Affiliations:
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Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Kurosu, Takeshi" sort="Kurosu, Takeshi" uniqKey="Kurosu T" first="Takeshi" last="Kurosu">Takeshi Kurosu</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shimojima, Masayuki" sort="Shimojima, Masayuki" uniqKey="Shimojima M" first="Masayuki" last="Shimojima">Masayuki Shimojima</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology III, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology III, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Melaku, Simenew Keskes" sort="Melaku, Simenew Keskes" uniqKey="Melaku S" first="Simenew Keskes" last="Melaku">Simenew Keskes Melaku</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Ethiopia</nlm:aff><country xml:lang="fr">Éthiopie</country><wicri:regionArea>Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University</wicri:regionArea><wicri:noRegion>Addis Ababa Science and Technology University</wicri:noRegion></affiliation></author><author><name sortKey="Sentsui, Hiroshi" sort="Sentsui, Hiroshi" uniqKey="Sentsui H" first="Hiroshi" last="Sentsui">Hiroshi Sentsui</name><affiliation wicri:level="1"><nlm:aff id="aff0030">Department of Veterinary Medicine, Nihon University, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Veterinary Medicine, Nihon University</wicri:regionArea><wicri:noRegion>Nihon University</wicri:noRegion></affiliation></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28993122</idno><idno type="pmc">7113858</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113858</idno><idno type="RBID">PMC:7113858</idno><idno type="doi">10.1016/j.jviromet.2017.10.008</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000E93</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E93</idno><idno type="wicri:Area/Pmc/Curation">000E93</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E93</idno><idno type="wicri:Area/Pmc/Checkpoint">000913</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000913</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA</title><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Fukuma, Aiko" sort="Fukuma, Aiko" uniqKey="Fukuma A" first="Aiko" last="Fukuma">Aiko Fukuma</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Kurosu, Takeshi" sort="Kurosu, Takeshi" uniqKey="Kurosu T" first="Takeshi" last="Kurosu">Takeshi Kurosu</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shimojima, Masayuki" sort="Shimojima, Masayuki" uniqKey="Shimojima M" first="Masayuki" last="Shimojima">Masayuki Shimojima</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology III, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology III, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Melaku, Simenew Keskes" sort="Melaku, Simenew Keskes" uniqKey="Melaku S" first="Simenew Keskes" last="Melaku">Simenew Keskes Melaku</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Ethiopia</nlm:aff><country xml:lang="fr">Éthiopie</country><wicri:regionArea>Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University</wicri:regionArea><wicri:noRegion>Addis Ababa Science and Technology University</wicri:noRegion></affiliation></author><author><name sortKey="Sentsui, Hiroshi" sort="Sentsui, Hiroshi" uniqKey="Sentsui H" first="Hiroshi" last="Sentsui">Hiroshi Sentsui</name><affiliation wicri:level="1"><nlm:aff id="aff0030">Department of Veterinary Medicine, Nihon University, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Veterinary Medicine, Nihon University</wicri:regionArea><wicri:noRegion>Nihon University</wicri:noRegion></affiliation></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Novel monoclonal antibodies against MERS-CoV were produced and characterized.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Anti-MERS-CoV antibody detection system by competitive ELISA was developed.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The competitive ELISA was validated using sera taken from dromedary camels.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Hammadi, Z M" uniqKey="Al Hammadi Z">Z.M. Al Hammadi</name></author><author><name sortKey="Chu, D K" uniqKey="Chu D">D.K. Chu</name></author><author><name sortKey="Eltahir, Y M" uniqKey="Eltahir Y">Y.M. Eltahir</name></author><author><name sortKey="Al Hosani, F" uniqKey="Al Hosani F">F. Al Hosani</name></author><author><name sortKey="Al Mulla, M" uniqKey="Al Mulla M">M. Al Mulla</name></author><author><name sortKey="Tarnini, W" uniqKey="Tarnini W">W. Tarnini</name></author><author><name sortKey="Hall, A J" uniqKey="Hall A">A.J. Hall</name></author><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name></author><author><name sortKey="Abdelkhalek, M M" uniqKey="Abdelkhalek M">M.M. Abdelkhalek</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Al Muhairi, S S" uniqKey="Al Muhairi S">S.S. Al Muhairi</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Gethamy, M" uniqKey="Al Gethamy M">M. Al-Gethamy</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Hussain, R" uniqKey="Hussain R">R. Hussain</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blomstrom, A L" uniqKey="Blomstrom A">A.L. Blomstrom</name></author><author><name sortKey="Scharin, I" uniqKey="Scharin I">I. Scharin</name></author><author><name sortKey="Stenberg, H" uniqKey="Stenberg H">H. Stenberg</name></author><author><name sortKey="Figueiredo, J" uniqKey="Figueiredo J">J. Figueiredo</name></author><author><name sortKey="Nhambirre, O" uniqKey="Nhambirre O">O. Nhambirre</name></author><author><name sortKey="Abilio, A" uniqKey="Abilio A">A. Abilio</name></author><author><name sortKey="Berg, M" uniqKey="Berg M">M. Berg</name></author><author><name sortKey="Fafetine, J" uniqKey="Fafetine J">J. Fafetine</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chand, K" uniqKey="Chand K">K. Chand</name></author><author><name sortKey="Biswas, S K" uniqKey="Biswas S">S.K. Biswas</name></author><author><name sortKey="Pandey, A B" uniqKey="Pandey A">A.B. Pandey</name></author><author><name sortKey="Saxena, A" uniqKey="Saxena A">A. Saxena</name></author><author><name sortKey="Tewari, N" uniqKey="Tewari N">N. Tewari</name></author><author><name sortKey="Mondal, B" uniqKey="Mondal B">B. Mondal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Kang, Y" uniqKey="Kang Y">Y. Kang</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Luk, H K" uniqKey="Luk H">H.K. Luk</name></author><author><name sortKey="Poon, R W" uniqKey="Poon R">R.W. Poon</name></author><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. Chan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Xia, N" uniqKey="Xia N">N. Xia</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I. Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T. Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O. Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M. Eschbach-Bludau</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Gopal, R" uniqKey="Gopal R">R. Gopal</name></author><author><name sortKey="Ballhause, M" uniqKey="Ballhause M">M. Ballhause</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D. Muth</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Drexler, J F" uniqKey="Drexler J">J.F. Drexler</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M. Zambon</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R M" uniqKey="Fouchier R">R.M. Fouchier</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Al Masri, M" uniqKey="Al Masri M">M. Al-Masri</name></author><author><name sortKey="Hossain, R" uniqKey="Hossain R">R. Hossain</name></author><author><name sortKey="Madani, H" uniqKey="Madani H">H. Madani</name></author><author><name sortKey="Sieberg, A" uniqKey="Sieberg A">A. Sieberg</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Lattwein, E" uniqKey="Lattwein E">E. Lattwein</name></author><author><name sortKey="Alhakeem, R F" uniqKey="Alhakeem R">R.F. Alhakeem</name></author><author><name sortKey="Assiri, A M" uniqKey="Assiri A">A.M. Assiri</name></author><author><name sortKey="Hajomar, W" uniqKey="Hajomar W">W. Hajomar</name></author><author><name sortKey="Albarrak, A M" uniqKey="Albarrak A">A.M. Albarrak</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Zumla, A I" uniqKey="Zumla A">A.I. Zumla</name></author><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukuma, A" uniqKey="Fukuma A">A. Fukuma</name></author><author><name sortKey="Tani, H" uniqKey="Tani H">H. Tani</name></author><author><name sortKey="Taniguchi, S" uniqKey="Taniguchi S">S. Taniguchi</name></author><author><name sortKey="Shimojima, M" uniqKey="Shimojima M">M. Shimojima</name></author><author><name sortKey="Saijo, M" uniqKey="Saijo M">M. Saijo</name></author><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukuma, A" uniqKey="Fukuma A">A. Fukuma</name></author><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author><author><name sortKey="Yoshikawa, T" uniqKey="Yoshikawa T">T. Yoshikawa</name></author><author><name sortKey="Tani, H" uniqKey="Tani H">H. Tani</name></author><author><name sortKey="Taniguchi, S" uniqKey="Taniguchi S">S. Taniguchi</name></author><author><name sortKey="Kurosu, T" uniqKey="Kurosu T">T. Kurosu</name></author><author><name sortKey="Egawa, K" uniqKey="Egawa K">K. Egawa</name></author><author><name sortKey="Suda, Y" uniqKey="Suda Y">Y. Suda</name></author><author><name sortKey="Singh, H" uniqKey="Singh H">H. Singh</name></author><author><name sortKey="Nomachi, T" uniqKey="Nomachi T">T. Nomachi</name></author><author><name sortKey="Gokuden, M" uniqKey="Gokuden M">M. Gokuden</name></author><author><name sortKey="Ando, K" uniqKey="Ando K">K. Ando</name></author><author><name sortKey="Kida, K" uniqKey="Kida K">K. Kida</name></author><author><name sortKey="Kan, M" uniqKey="Kan M">M. Kan</name></author><author><name sortKey="Kato, N" uniqKey="Kato N">N. Kato</name></author><author><name sortKey="Yoshikawa, A" uniqKey="Yoshikawa A">A. Yoshikawa</name></author><author><name sortKey="Kitamoto, H" uniqKey="Kitamoto H">H. Kitamoto</name></author><author><name sortKey="Sato, Y" uniqKey="Sato Y">Y. Sato</name></author><author><name sortKey="Suzuki, T" uniqKey="Suzuki T">T. Suzuki</name></author><author><name sortKey="Hasegawa, H" uniqKey="Hasegawa H">H. Hasegawa</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author><author><name sortKey="Shimojima, M" uniqKey="Shimojima M">M. Shimojima</name></author><author><name sortKey="Saijo, M" uniqKey="Saijo M">M. Saijo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author><author><name sortKey="Mizutani, T" uniqKey="Mizutani T">T. Mizutani</name></author><author><name sortKey="Saijo, M" uniqKey="Saijo M">M. Saijo</name></author><author><name sortKey="Matsuyama, S" uniqKey="Matsuyama S">S. Matsuyama</name></author><author><name sortKey="Miyajima, N" uniqKey="Miyajima N">N. Miyajima</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name></author><author><name sortKey="Itamura, S" uniqKey="Itamura S">S. Itamura</name></author><author><name sortKey="Kurane, I" uniqKey="Kurane I">I. Kurane</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author><author><name sortKey="Mizutani, T" uniqKey="Mizutani T">T. Mizutani</name></author><author><name sortKey="Saijo, M" uniqKey="Saijo M">M. Saijo</name></author><author><name sortKey="Kurane, I" uniqKey="Kurane I">I. Kurane</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M. Tashiro</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hemida, M G" uniqKey="Hemida M">M.G. Hemida</name></author><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name></author><author><name sortKey="Al Jassim, R A" uniqKey="Al Jassim R">R.A. Al Jassim</name></author><author><name sortKey="Kayali, G" uniqKey="Kayali G">G. Kayali</name></author><author><name sortKey="Siu, L Y" uniqKey="Siu L">L.Y. Siu</name></author><author><name sortKey="Wang, P" uniqKey="Wang P">P. Wang</name></author><author><name sortKey="Chu, K W" uniqKey="Chu K">K.W. Chu</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Ali, M A" uniqKey="Ali M">M.A. Ali</name></author><author><name sortKey="Alnaeem, A" uniqKey="Alnaeem A">A. Alnaeem</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Saif, L" uniqKey="Saif L">L. Saif</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hirota, J" uniqKey="Hirota J">J. Hirota</name></author><author><name sortKey="Shimoji, Y" uniqKey="Shimoji Y">Y. Shimoji</name></author><author><name sortKey="Shimizu, S" uniqKey="Shimizu S">S. Shimizu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houlihan, C F" uniqKey="Houlihan C">C.F. Houlihan</name></author><author><name sortKey="Mcgowan, C R" uniqKey="Mcgowan C">C.R. McGowan</name></author><author><name sortKey="Dicks, S" uniqKey="Dicks S">S. Dicks</name></author><author><name sortKey="Baguelin, M" uniqKey="Baguelin M">M. Baguelin</name></author><author><name sortKey="Moore, D A J" uniqKey="Moore D">D.A.J. Moore</name></author><author><name sortKey="Mabey, D" uniqKey="Mabey D">D. Mabey</name></author><author><name sortKey="Roberts, C H" uniqKey="Roberts C">C.H. Roberts</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A. Kumar</name></author><author><name sortKey="Samuel, D" uniqKey="Samuel D">D. Samuel</name></author><author><name sortKey="Tedder, R" uniqKey="Tedder R">R. Tedder</name></author><author><name sortKey="Glynn, J R" uniqKey="Glynn J">J.R. Glynn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishii, K" uniqKey="Ishii K">K. Ishii</name></author><author><name sortKey="Hasegawa, H" uniqKey="Hasegawa H">H. Hasegawa</name></author><author><name sortKey="Nagata, N" uniqKey="Nagata N">N. Nagata</name></author><author><name sortKey="Ami, Y" uniqKey="Ami Y">Y. Ami</name></author><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name></author><author><name sortKey="Tsunetsugu Yokota, Y" uniqKey="Tsunetsugu Yokota Y">Y. Tsunetsugu-Yokota</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Iwata Yoshikawa, N" uniqKey="Iwata Yoshikawa N">N. Iwata-Yoshikawa</name></author><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S. Fukushi</name></author><author><name sortKey="Fukuma, A" uniqKey="Fukuma A">A. Fukuma</name></author><author><name sortKey="Suzuki, T" uniqKey="Suzuki T">T. Suzuki</name></author><author><name sortKey="Takeda, M" uniqKey="Takeda M">M. Takeda</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M. Tashiro</name></author><author><name sortKey="Hasegawa, H" uniqKey="Hasegawa H">H. Hasegawa</name></author><author><name sortKey="Nagata, N" uniqKey="Nagata N">N. Nagata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Liu, D" uniqKey="Liu D">D. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name></author><author><name sortKey="Al Rabeeah, A A" uniqKey="Al Rabeeah A">A.A. Al-Rabeeah</name></author><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name></author><author><name sortKey="Alhakeem, R F" uniqKey="Alhakeem R">R.F. Alhakeem</name></author><author><name sortKey="Alrabiah, F A" uniqKey="Alrabiah F">F.A. AlRabiah</name></author><author><name sortKey="Al Hajjar, S" uniqKey="Al Hajjar S">S. Al Hajjar</name></author><author><name sortKey="Albarrak, A" uniqKey="Albarrak A">A. Albarrak</name></author><author><name sortKey="Flemban, H" uniqKey="Flemban H">H. Flemban</name></author><author><name sortKey="Balkhy, H" uniqKey="Balkhy H">H. Balkhy</name></author><author><name sortKey="Barry, M" uniqKey="Barry M">M. Barry</name></author><author><name sortKey="Alhassan, S" uniqKey="Alhassan S">S. Alhassan</name></author><author><name sortKey="Alsubaie, S" uniqKey="Alsubaie S">S. Alsubaie</name></author><author><name sortKey="Zumla, A" uniqKey="Zumla A">A. Zumla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moreno, A" uniqKey="Moreno A">A. Moreno</name></author><author><name sortKey="Lelli, D" uniqKey="Lelli D">D. Lelli</name></author><author><name sortKey="Brocchi, E" uniqKey="Brocchi E">E. Brocchi</name></author><author><name sortKey="Sozzi, E" uniqKey="Sozzi E">E. Sozzi</name></author><author><name sortKey="Vinco, L J" uniqKey="Vinco L">L.J. Vinco</name></author><author><name sortKey="Grilli, G" uniqKey="Grilli G">G. Grilli</name></author><author><name sortKey="Cordioli, P" uniqKey="Cordioli P">P. Cordioli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Al Masri, M" uniqKey="Al Masri M">M. Al-Masri</name></author><author><name sortKey="Turkestani, A" uniqKey="Turkestani A">A. Turkestani</name></author><author><name sortKey="Ritz, D" uniqKey="Ritz D">D. Ritz</name></author><author><name sortKey="Sieberg, A" uniqKey="Sieberg A">A. Sieberg</name></author><author><name sortKey="Aldabbagh, S" uniqKey="Aldabbagh S">S. Aldabbagh</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Lattwein, E" uniqKey="Lattwein E">E. Lattwein</name></author><author><name sortKey="Alhakeem, R F" uniqKey="Alhakeem R">R.F. Alhakeem</name></author><author><name sortKey="Assiri, A M" uniqKey="Assiri A">A.M. Assiri</name></author><author><name sortKey="Albarrak, A M" uniqKey="Albarrak A">A.M. Albarrak</name></author><author><name sortKey="Al Shangiti, A M" uniqKey="Al Shangiti A">A.M. Al-Shangiti</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Wikramaratna, P" uniqKey="Wikramaratna P">P. Wikramaratna</name></author><author><name sortKey="Alrabeeah, A A" uniqKey="Alrabeeah A">A.A. Alrabeeah</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name></author><author><name sortKey="Wang, P" uniqKey="Wang P">P. Wang</name></author><author><name sortKey="Gomaa, M R" uniqKey="Gomaa M">M.R. Gomaa</name></author><author><name sortKey="El Shesheny, R" uniqKey="El Shesheny R">R. El-Shesheny</name></author><author><name sortKey="Kandeil, A" uniqKey="Kandeil A">A. Kandeil</name></author><author><name sortKey="Bagato, O" uniqKey="Bagato O">O. Bagato</name></author><author><name sortKey="Siu, L Y" uniqKey="Siu L">L.Y. Siu</name></author><author><name sortKey="Shehata, M M" uniqKey="Shehata M">M.M. Shehata</name></author><author><name sortKey="Kayed, A S" uniqKey="Kayed A">A.S. Kayed</name></author><author><name sortKey="Moatasim, Y" uniqKey="Moatasim Y">Y. Moatasim</name></author><author><name sortKey="Li, M" uniqKey="Li M">M. Li</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Webby, R J" uniqKey="Webby R">R.J. Webby</name></author><author><name sortKey="Ali, M A" uniqKey="Ali M">M.A. Ali</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Kayali, G" uniqKey="Kayali G">G. Kayali</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qing, T" uniqKey="Qing T">T. Qing</name></author><author><name sortKey="Saijo, M" uniqKey="Saijo M">M. Saijo</name></author><author><name sortKey="Lei, H" uniqKey="Lei H">H. Lei</name></author><author><name sortKey="Niikura, M" uniqKey="Niikura M">M. Niikura</name></author><author><name sortKey="Maeda, A" uniqKey="Maeda A">A. Maeda</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T. Ikegami</name></author><author><name sortKey="Xinjung, W" uniqKey="Xinjung W">W. Xinjung</name></author><author><name sortKey="Kurane, I" uniqKey="Kurane I">I. Kurane</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C" uniqKey="Reusken C">C. Reusken</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H. Mou</name></author><author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.J. Godeke</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Haagmans, B" uniqKey="Haagmans B">B. Haagmans</name></author><author><name sortKey="De Sousa, R" uniqKey="De Sousa R">R. de Sousa</name></author><author><name sortKey="Schuurman, N" uniqKey="Schuurman N">N. Schuurman</name></author><author><name sortKey="Dittmer, U" uniqKey="Dittmer U">U. Dittmer</name></author><author><name sortKey="Rottier, P" uniqKey="Rottier P">P. Rottier</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A. Osterhaus</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Koopmans, M" uniqKey="Koopmans M">M. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Gutierrez, C" uniqKey="Gutierrez C">C. Gutierrez</name></author><author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.J. Godeke</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D. Muth</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Smits De Vries, L" uniqKey="Smits De Vries L">L. Smits-De Vries</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Drexler, J F" uniqKey="Drexler J">J.F. Drexler</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="El Tahir, Y E" uniqKey="El Tahir Y">Y.E. El Tahir</name></author><author><name sortKey="De Sousa, R" uniqKey="De Sousa R">R. De Sousa</name></author><author><name sortKey="Van Beek, J" uniqKey="Van Beek J">J. van Beek</name></author><author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N. Nowotny</name></author><author><name sortKey="Van Maanen, K" uniqKey="Van Maanen K">K. van Maanen</name></author><author><name sortKey="Hidalgo Hermoso, E" uniqKey="Hidalgo Hermoso E">E. Hidalgo-Hermoso</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Rottier, P" uniqKey="Rottier P">P. Rottier</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A. Osterhaus</name></author><author><name sortKey="Gortazar Schmidt, C" uniqKey="Gortazar Schmidt C">C. Gortazar-Schmidt</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Messadi, L" uniqKey="Messadi L">L. Messadi</name></author><author><name sortKey="Feyisa, A" uniqKey="Feyisa A">A. Feyisa</name></author><author><name sortKey="Ularamu, H" uniqKey="Ularamu H">H. Ularamu</name></author><author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.J. Godeke</name></author><author><name sortKey="Danmarwa, A" uniqKey="Danmarwa A">A. Danmarwa</name></author><author><name sortKey="Dawo, F" uniqKey="Dawo F">F. Dawo</name></author><author><name sortKey="Jemli, M" uniqKey="Jemli M">M. Jemli</name></author><author><name sortKey="Melaku, S" uniqKey="Melaku S">S. Melaku</name></author><author><name sortKey="Shamaki, D" uniqKey="Shamaki D">D. Shamaki</name></author><author><name sortKey="Woma, Y" uniqKey="Woma Y">Y. Woma</name></author><author><name sortKey="Wungak, Y" uniqKey="Wungak Y">Y. Wungak</name></author><author><name sortKey="Gebremedhin, E Z" uniqKey="Gebremedhin E">E.Z. Gebremedhin</name></author><author><name sortKey="Zutt, I" uniqKey="Zutt I">I. Zutt</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shirato, K" uniqKey="Shirato K">K. Shirato</name></author><author><name sortKey="Azumano, A" uniqKey="Azumano A">A. Azumano</name></author><author><name sortKey="Nakao, T" uniqKey="Nakao T">T. Nakao</name></author><author><name sortKey="Hagihara, D" uniqKey="Hagihara D">D. Hagihara</name></author><author><name sortKey="Ishida, M" uniqKey="Ishida M">M. Ishida</name></author><author><name sortKey="Tamai, K" uniqKey="Tamai K">K. Tamai</name></author><author><name sortKey="Yamazaki, K" uniqKey="Yamazaki K">K. Yamazaki</name></author><author><name sortKey="Kawase, M" uniqKey="Kawase M">M. Kawase</name></author><author><name sortKey="Okamoto, Y" uniqKey="Okamoto Y">Y. Okamoto</name></author><author><name sortKey="Kawakami, S" uniqKey="Kawakami S">S. Kawakami</name></author><author><name sortKey="Okada, N" uniqKey="Okada N">N. Okada</name></author><author><name sortKey="Fukushima, K" uniqKey="Fukushima K">K. Fukushima</name></author><author><name sortKey="Nakajima, K" uniqKey="Nakajima K">K. Nakajima</name></author><author><name sortKey="Matsuyama, S" uniqKey="Matsuyama S">S. Matsuyama</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S. Zhang</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Tong, P" uniqKey="Tong P">P. Tong</name></author><author><name sortKey="Guo, D" uniqKey="Guo D">D. Guo</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L. Fu</name></author><author><name sortKey="Cui, Y" uniqKey="Cui Y">Y. Cui</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Arledge, K C" uniqKey="Arledge K">K.C. Arledge</name></author><author><name sortKey="Chen, Y H" uniqKey="Chen Y">Y.H. Chen</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Who Mers Cov Research, G" uniqKey="Who Mers Cov Research G">G. Who Mers-Cov Research</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="Prabakaran, P" uniqKey="Prabakaran P">P. Prabakaran</name></author><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W. Shi</name></author><author><name sortKey="Feng, Y" uniqKey="Feng Y">Y. Feng</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Zhou, T" uniqKey="Zhou T">T. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name></author><author><name sortKey="Baker, S C" uniqKey="Baker S">S.C. Baker</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Brown, C S" uniqKey="Brown C">C.S. Brown</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Galiano, M" uniqKey="Galiano M">M. Galiano</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author><author><name sortKey="Stephens, G M" uniqKey="Stephens G">G.M. Stephens</name></author><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M. Zambon</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol Methods</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol. Methods</journal-id><journal-title-group><journal-title>Journal of Virological Methods</journal-title></journal-title-group><issn pub-type="ppub">0166-0934</issn><issn pub-type="epub">1879-0984</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28993122</article-id><article-id pub-id-type="pmc">7113858</article-id><article-id pub-id-type="publisher-id">S0166-0934(17)30582-7</article-id><article-id pub-id-type="doi">10.1016/j.jviromet.2017.10.008</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Fukushi</surname><given-names>Shuetsu</given-names></name><email>fukushi@nih.go.jp</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Fukuma</surname><given-names>Aiko</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Kurosu</surname><given-names>Takeshi</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Watanabe</surname><given-names>Shumpei</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Shimojima</surname><given-names>Masayuki</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Shirato</surname><given-names>Kazuya</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Iwata-Yoshikawa</surname><given-names>Naoko</given-names></name><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Nagata</surname><given-names>Noriyo</given-names></name><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0045"><name><surname>Ohnishi</surname><given-names>Kazuo</given-names></name><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0050"><name><surname>Ato</surname><given-names>Manabu</given-names></name><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0055"><name><surname>Melaku</surname><given-names>Simenew Keskes</given-names></name><xref rid="aff0025" ref-type="aff">e</xref></contrib><contrib contrib-type="author" id="aut0060"><name><surname>Sentsui</surname><given-names>Hiroshi</given-names></name><xref rid="aff0030" ref-type="aff">f</xref></contrib><contrib contrib-type="author" id="aut0065"><name><surname>Saijo</surname><given-names>Masayuki</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Department of Virology I, National Institute of Infectious Diseases, Japan</aff><aff id="aff0010"><label>b</label>Department of Virology III, National Institute of Infectious Diseases, Japan</aff><aff id="aff0015"><label>c</label>Department of Pathology, National Institute of Infectious Diseases, Japan</aff><aff id="aff0020"><label>d</label>Department of Immunology, National Institute of Infectious Diseases, Japan</aff><aff id="aff0025"><label>e</label>Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Ethiopia</aff><aff id="aff0030"><label>f</label>Department of Veterinary Medicine, Nihon University, Japan</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. <email>fukushi@nih.go.jp</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>6</day><month>10</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>1</month><year>2018</year></pub-date><pub-date pub-type="epub"><day>6</day><month>10</month><year>2017</year></pub-date><volume>251</volume><fpage>22</fpage><lpage>29</lpage><history><date date-type="received"><day>12</day><month>9</month><year>2017</year></date><date date-type="rev-recd"><day>5</day><month>10</month><year>2017</year></date><date date-type="accepted"><day>6</day><month>10</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="abs0005"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Novel monoclonal antibodies against MERS-CoV were produced and characterized.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Anti-MERS-CoV antibody detection system by competitive ELISA was developed.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The competitive ELISA was validated using sera taken from dromedary camels.</p></list-item></list></p></abstract><abstract id="abs0010"><p>Since discovering the Middle East respiratory syndrome coronavirus (MERS-CoV) as a causative agent of severe respiratory illness in the Middle East in 2012, serological testing has been conducted to assess antibody responses in patients and to investigate the zoonotic reservoir of the virus. Although the virus neutralization test is the gold standard assay for MERS diagnosis and for investigating the zoonotic reservoir, it uses live virus and so must be performed in high containment laboratories. Competitive ELISA (cELISA), in which a labeled monoclonal antibody (MAb) competes with test serum antibodies for target epitopes, may be a suitable alternative because it detects antibodies in a species-independent manner. In this study, novel MAbs against the spike protein of MERS-CoV were produced and characterized. One of these MAbs was used to develop a cELISA. The cELISA detected MERS-CoV-specific antibodies in sera from MERS-CoV-infected rats and rabbits immunized with the spike protein of MERS-CoV. The MAb-based cELISA was validated using sera from Ethiopian dromedary camels. Relative to the neutralization test, the cELISA detected MERS-CoV-specific antibodies in 66 Ethiopian dromedary camels with a sensitivity and specificity of 98% and 100%, respectively. The cELISA and neutralization test results correlated well (Pearson’s correlation coefficients = 0.71–0.76, depending on the cELISA serum dilution). This cELISA may be useful for MERS epidemiological investigations on MERS-CoV infection.</p></abstract><kwd-group id="kwd0005"><title>Keywords</title><kwd>MERS coronavirus</kwd><kwd>Epidemiology</kwd><kwd>Neutralizing antibody</kwd><kwd>Competitive ELISA</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Japon</li><li>Éthiopie</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></noRegion><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><name sortKey="Fukuma, Aiko" sort="Fukuma, Aiko" uniqKey="Fukuma A" first="Aiko" last="Fukuma">Aiko Fukuma</name><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><name sortKey="Kurosu, Takeshi" sort="Kurosu, Takeshi" uniqKey="Kurosu T" first="Takeshi" last="Kurosu">Takeshi Kurosu</name><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><name sortKey="Sentsui, Hiroshi" sort="Sentsui, Hiroshi" uniqKey="Sentsui H" first="Hiroshi" last="Sentsui">Hiroshi Sentsui</name><name sortKey="Shimojima, Masayuki" sort="Shimojima, Masayuki" uniqKey="Shimojima M" first="Masayuki" last="Shimojima">Masayuki Shimojima</name><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name></country><country name="Éthiopie"><noRegion><name sortKey="Melaku, Simenew Keskes" sort="Melaku, Simenew Keskes" uniqKey="Melaku S" first="Simenew Keskes" last="Melaku">Simenew Keskes Melaku</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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