Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1
Identifieur interne : 000905 ( Pmc/Checkpoint ); précédent : 000904; suivant : 000906Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1
Auteurs : Ali A. Rabaan [Arabie saoudite] ; Ali M. Bazzi [Arabie saoudite] ; Sana A. Alshaikh [Arabie saoudite]Source :
- Diagnostic Microbiology and Infectious Disease [ 0732-8893 ] ; 2017.
Abstract
To compare two influenza polymerase chain reaction (PCR) methods.
A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.
There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0–19-year age group) but significantly lower both for older patients (68.2% for 60–79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).
Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.
Url:
DOI: 10.1016/j.diagmicrobio.2017.12.010
PubMed: 29329754
PubMed Central: 7132748
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1</title><author><name sortKey="Rabaan, Ali A" sort="Rabaan, Ali A" uniqKey="Rabaan A" first="Ali A." last="Rabaan">Ali A. Rabaan</name><affiliation wicri:level="1"><nlm:aff id="af0005">Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Bazzi, Ali M" sort="Bazzi, Ali M" uniqKey="Bazzi A" first="Ali M." last="Bazzi">Ali M. Bazzi</name><affiliation wicri:level="1"><nlm:aff id="af0010">Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. Alshaikh</name><affiliation wicri:level="1"><nlm:aff id="af0015">Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675</wicri:regionArea><wicri:noRegion>31675</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29329754</idno><idno type="pmc">7132748</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132748</idno><idno type="RBID">PMC:7132748</idno><idno type="doi">10.1016/j.diagmicrobio.2017.12.010</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000C03</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C03</idno><idno type="wicri:Area/Pmc/Curation">000C03</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C03</idno><idno type="wicri:Area/Pmc/Checkpoint">000905</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000905</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1</title><author><name sortKey="Rabaan, Ali A" sort="Rabaan, Ali A" uniqKey="Rabaan A" first="Ali A." last="Rabaan">Ali A. Rabaan</name><affiliation wicri:level="1"><nlm:aff id="af0005">Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Bazzi, Ali M" sort="Bazzi, Ali M" uniqKey="Bazzi A" first="Ali M." last="Bazzi">Ali M. Bazzi</name><affiliation wicri:level="1"><nlm:aff id="af0010">Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311</wicri:regionArea><wicri:noRegion>Dhahran 31311</wicri:noRegion></affiliation></author><author><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. Alshaikh</name><affiliation wicri:level="1"><nlm:aff id="af0015">Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675</wicri:regionArea><wicri:noRegion>31675</wicri:noRegion></affiliation></author></analytic><series><title level="j">Diagnostic Microbiology and Infectious Disease</title><idno type="ISSN">0732-8893</idno><idno type="eISSN">1879-0070</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Objective</title><p>To compare two influenza polymerase chain reaction (PCR) methods.</p></sec><sec><title>Methods</title><p>A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.</p></sec><sec><title>Results</title><p>There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0–19-year age group) but significantly lower both for older patients (68.2% for 60–79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).</p></sec><sec><title>Conclusions</title><p>Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Tawfiq, J" uniqKey="Al Tawfiq J">J. Al-Tawfiq</name></author><author><name sortKey="Abed, M" uniqKey="Abed M">M. Abed</name></author><author><name sortKey="Saadeh, B M" uniqKey="Saadeh B">B.M. Saadeh</name></author><author><name sortKey="Ghandour, J" uniqKey="Ghandour J">J. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Diagn Microbiol Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Diagn. Microbiol. Infect. Dis</journal-id><journal-title-group><journal-title>Diagnostic Microbiology and Infectious Disease</journal-title></journal-title-group><issn pub-type="ppub">0732-8893</issn><issn pub-type="epub">1879-0070</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">29329754</article-id><article-id pub-id-type="pmc">7132748</article-id><article-id pub-id-type="publisher-id">S0732-8893(17)30406-6</article-id><article-id pub-id-type="doi">10.1016/j.diagmicrobio.2017.12.010</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1</article-title></title-group><contrib-group><contrib contrib-type="author" id="au0005"><name><surname>Rabaan</surname><given-names>Ali A.</given-names></name><email>arabaan@gmail.com</email><email>ali.rabaan@jhah.com</email><xref rid="af0005" ref-type="aff">a</xref><xref rid="cr0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="au0010"><name><surname>Bazzi</surname><given-names>Ali M.</given-names></name><xref rid="af0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au0015"><name><surname>Alshaikh</surname><given-names>Sana A.</given-names></name><xref rid="af0015" ref-type="aff">c</xref></contrib></contrib-group><aff id="af0005"><label>a</label>Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</aff><aff id="af0010"><label>b</label>Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia</aff><aff id="af0015"><label>c</label>Molecular Diagnostic laboratory, Dammam Regional Laboratory, Ministry of Health, Dammam, 31675, Saudi Arabia</aff><author-notes><corresp id="cr0005"><label>⁎</label>Corresponding author. Tel.: +966-13-877-6670; fax: +966-13-877-6741. <email>arabaan@gmail.com</email><email>ali.rabaan@jhah.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>16</day><month>12</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>4</month><year>2018</year></pub-date><pub-date pub-type="epub"><day>16</day><month>12</month><year>2017</year></pub-date><volume>90</volume><issue>4</issue><fpage>280</fpage><lpage>285</lpage><history><date date-type="received"><day>1</day><month>9</month><year>2017</year></date><date date-type="rev-recd"><day>11</day><month>12</month><year>2017</year></date><date date-type="accepted"><day>13</day><month>12</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="ab0005"><sec><title>Objective</title><p>To compare two influenza polymerase chain reaction (PCR) methods.</p></sec><sec><title>Methods</title><p>A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.</p></sec><sec><title>Results</title><p>There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0–19-year age group) but significantly lower both for older patients (68.2% for 60–79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).</p></sec><sec><title>Conclusions</title><p>Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.</p></sec></abstract><abstract abstract-type="author-highlights" id="ab0010"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="li0005"><label>•</label><p id="p0005">92.12% agreement between assays for FluA/FluA (H1N1) pdm09 detection</p></list-item><list-item id="li0010"><label>•</label><p id="p0010">Specificity of FluA/FluA (H1N1) pdm09 detection high for Cepheid assay</p></list-item><list-item id="li0015"><label>•</label><p id="p0015">Sensitivity of total FluA detection significantly lower in males and older patients</p></list-item></list></p></abstract><kwd-group id="ks0005"><title>Keywords</title><kwd>Cepheid® Xpert flu</kwd><kwd>Influenza A</kwd><kwd>Influenza A (H1N1) pdm09</kwd><kwd>Real-time PCR</kwd><kwd>Saudi Arabia</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Rabaan, Ali A" sort="Rabaan, Ali A" uniqKey="Rabaan A" first="Ali A." last="Rabaan">Ali A. Rabaan</name></noRegion><name sortKey="Alshaikh, Sana A" sort="Alshaikh, Sana A" uniqKey="Alshaikh S" first="Sana A." last="Alshaikh">Sana A. Alshaikh</name><name sortKey="Bazzi, Ali M" sort="Bazzi, Ali M" uniqKey="Bazzi A" first="Ali M." last="Bazzi">Ali M. Bazzi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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