Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient
Identifieur interne : 000444 ( Pmc/Checkpoint ); précédent : 000443; suivant : 000445Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient
Auteurs : Peihua Niu [République populaire de Chine] ; Senyan Zhang [République populaire de Chine] ; Panpan Zhou [République populaire de Chine] ; Baoying Huang [République populaire de Chine] ; Yao Deng [République populaire de Chine] ; Kun Qin [République populaire de Chine] ; Pengfei Wang [République populaire de Chine] ; Wenling Wang [République populaire de Chine] ; Xinquan Wang [République populaire de Chine] ; Jianfang Zhou [République populaire de Chine] ; Linqi Zhang [République populaire de Chine] ; Wenjie Tan [République populaire de Chine]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2018.
Abstract
We describe the MERS-CoV-neutralizing antibody repertoires from a survivor and characterized 13 ultrapotent mAbs. The mAbs, MERS-GD27 and MERS-GD33, exhibited the strongest neutralizing activities. Both targeted receptor-binding domain via different contacts. The epitopes of MERS-GD27 overlapped with DPP4-binding sites.
Url:
DOI: 10.1093/infdis/jiy311
PubMed: 29846635
PubMed Central: 7107445
Affiliations:
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Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Wenling" sort="Wang, Wenling" uniqKey="Wang W" first="Wenling" last="Wang">Wenling Wang</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Xinquan" sort="Wang, Xinquan" uniqKey="Wang X" first="Xinquan" last="Wang">Xinquan Wang</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Jianfang" sort="Zhou, Jianfang" uniqKey="Zhou J" first="Jianfang" last="Zhou">Jianfang Zhou</name><affiliation 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From a Recovered Patient</title><author><name sortKey="Niu, Peihua" sort="Niu, Peihua" uniqKey="Niu P" first="Peihua" last="Niu">Peihua Niu</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Senyan" sort="Zhang, Senyan" uniqKey="Zhang S" first="Senyan" last="Zhang">Senyan Zhang</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Panpan" sort="Zhou, Panpan" uniqKey="Zhou P" first="Panpan" last="Zhou">Panpan Zhou</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Baoying" sort="Huang, Baoying" uniqKey="Huang B" first="Baoying" last="Huang">Baoying Huang</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Yao" sort="Deng, Yao" uniqKey="Deng Y" first="Yao" last="Deng">Yao Deng</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qin, Kun" sort="Qin, Kun" uniqKey="Qin K" first="Kun" last="Qin">Kun Qin</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Pengfei" sort="Wang, Pengfei" uniqKey="Wang P" first="Pengfei" last="Wang">Pengfei Wang</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Wenling" sort="Wang, Wenling" uniqKey="Wang W" first="Wenling" last="Wang">Wenling Wang</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Xinquan" sort="Wang, Xinquan" uniqKey="Wang X" first="Xinquan" last="Wang">Xinquan Wang</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Jianfang" sort="Zhou, Jianfang" uniqKey="Zhou J" first="Jianfang" last="Zhou">Jianfang Zhou</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name><affiliation wicri:level="1"><nlm:aff id="AF0002">The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University</wicri:regionArea><wicri:noRegion>Tsinghua University</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><affiliation wicri:level="3"><nlm:aff id="AF0001">MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We describe the MERS-CoV-neutralizing antibody repertoires from a survivor and characterized 13 ultrapotent mAbs. The mAbs, MERS-GD27 and MERS-GD33, exhibited the strongest neutralizing activities. Both targeted receptor-binding domain via different contacts. The epitopes of MERS-GD27 overlapped with DPP4-binding sites.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D Corti</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Pedotti, M" uniqKey="Pedotti M">M Pedotti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Liu, D" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">J. Infect. Dis</journal-id><journal-id journal-id-type="publisher-id">jid</journal-id><journal-title-group><journal-title>The Journal of Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">0022-1899</issn><issn pub-type="epub">1537-6613</issn><publisher><publisher-name>Oxford University Press</publisher-name><publisher-loc>US</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">29846635</article-id><article-id pub-id-type="pmc">7107445</article-id><article-id pub-id-type="doi">10.1093/infdis/jiy311</article-id><article-id pub-id-type="publisher-id">jiy311</article-id><article-categories><subj-group subj-group-type="heading"><subject>Major Articles and Brief Reports</subject><subj-group subj-group-type="category-toc-heading"><subject>Viruses</subject></subj-group></subj-group></article-categories><title-group><article-title>Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Niu</surname><given-names>Peihua</given-names></name><xref ref-type="aff" rid="AF0001">1</xref><xref ref-type="author-notes" rid="fn-0010"></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Senyan</given-names></name><xref ref-type="aff" rid="AF0002">2</xref><xref ref-type="author-notes" rid="fn-0010"></xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Panpan</given-names></name><xref ref-type="aff" rid="AF0002">2</xref><xref ref-type="author-notes" rid="fn-0010"></xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Baoying</given-names></name><xref ref-type="aff" rid="AF0001">1</xref><xref ref-type="author-notes" rid="fn-0010"></xref></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Yao</given-names></name><xref ref-type="aff" rid="AF0001">1</xref></contrib><contrib contrib-type="author"><name><surname>Qin</surname><given-names>Kun</given-names></name><xref ref-type="aff" rid="AF0001">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Pengfei</given-names></name><xref ref-type="aff" rid="AF0002">2</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Wenling</given-names></name><xref ref-type="aff" rid="AF0001">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xinquan</given-names></name><xref ref-type="aff" rid="AF0002">2</xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Jianfang</given-names></name><xref ref-type="aff" rid="AF0001">1</xref><xref ref-type="author-notes" rid="fn-0020"></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Linqi</given-names></name><xref ref-type="aff" rid="AF0002">2</xref><xref ref-type="author-notes" rid="fn-0020"></xref></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Wenjie</given-names></name><xref ref-type="aff" rid="AF0001">1</xref><xref ref-type="author-notes" rid="fn-0020"></xref><xref ref-type="corresp" rid="c1"></xref><pmc-comment>tanwj28@163.com </pmc-comment>
</contrib></contrib-group><aff id="AF0001"><label>1</label>MOH Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China</aff><aff id="AF0002"><label>2</label>The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, China</aff><author-notes><corresp id="c1">Correspondence: W. Tan, MD, PhD, Key Laboratory of Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping district, Beijing, China (<email>tanwj28@163.com</email>).</corresp><fn id="fn-0010"><p>P. N., S. Z., P. Z., and B. H. contributed equally to this work.</p></fn><fn id="fn-0020"><p>J. Zhou, L. Zhang and W. Tan contributed equally.</p></fn></author-notes><pub-date pub-type="ppub"><day>15</day><month>10</month><year>2018</year></pub-date><pub-date pub-type="epub" iso-8601-date="2018-05-28"><day>28</day><month>5</month><year>2018</year></pub-date><pub-date pub-type="pmc-release"><day>08</day><month>9</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 12 months and
0 days and was based on the
<volume>218</volume><issue>8</issue><fpage>1249</fpage><lpage>1260</lpage><history><date date-type="received"><day>28</day><month>5</month><year>2018</year></date><date date-type="accepted"><day>23</day><month>5</month><year>2018</year></date></history><permissions><copyright-statement>© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.</copyright-statement><copyright-year>2018</copyright-year><license license-type="oup-standard" xlink:href="https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"><license-p>This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (<ext-link ext-link-type="uri" xlink:href="https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model">https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model</ext-link>)</license-p></license><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.</license-p></license></permissions><self-uri xlink:href="jiy311.pdf"></self-uri><abstract abstract-type="teaser"><p>We describe the MERS-CoV-neutralizing antibody repertoires from a survivor and characterized 13 ultrapotent mAbs. The mAbs, MERS-GD27 and MERS-GD33, exhibited the strongest neutralizing activities. Both targeted receptor-binding domain via different contacts. The epitopes of MERS-GD27 overlapped with DPP4-binding sites.</p></abstract><abstract><title>Abstract</title><sec id="s1"><title>Background</title><p>The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory infection with a high (~35%) mortality rate. Neutralizing antibodies targeting the spike of MERS-CoV have been shown to be a therapeutic option for treatment of lethal disease.</p></sec><sec id="s2"><title>Methods</title><p>We describe the germline diversity and neutralizing activity of 13 potent human monoclonal antibodies (mAbs) that target the MERS-CoV spike (S) protein. Biological functions were assessed by live MERS-CoV, pseudotype particle and its variants, and structural basis was also determined by crystallographic analysis.</p></sec><sec id="s3"><title>Results</title><p>Of the 13 mAbs displaying strong neutralizing activity against MERS-CoV, two with the immunoglobulin heavy-chain variable region (IGHV)1-69-derived heavy chain (named MERS-GD27 and MERS-GD33) showed the most potent neutralizing activity against pseudotyped and live MERS-CoV in vitro. Mutagenesis analysis suggested that MERS-GD27 and MERS-GD33 recognized distinct regions in S glycoproteins, and the combination of 2 mAbs demonstrated a synergistic effect in neutralization against pseudotyped MERS-CoV. The structural basis of MERS-GD27 neutralization and recognition revealed that its epitope almost completely overlapped with the receptor-binding site.</p></sec><sec id="s4"><title>Conclusions</title><p>Our data provide new insights into the specific antibody repertoires and the molecular determinants of neutralization during natural MERS-CoV infection in humans. This finding supports additional efforts to design and develop novel therapies to combat MERS-CoV infections in humans.</p></sec></abstract><kwd-group><kwd>crystallographic analysis</kwd><kwd>human monoclonal antibody</kwd><kwd>MERS-CoV</kwd><kwd>neutralizing antibody repertoires</kwd></kwd-group><funding-group><award-group award-type="grant"><funding-source><named-content content-type="funder-name">National Key Research and Development Program of China</named-content></funding-source><award-id>2016YFD0500300</award-id><award-id>2016YFC1200901</award-id><award-id>2016YFC1200200</award-id></award-group><award-group award-type="grant"><funding-source><named-content content-type="funder-name">Megaproject for Infectious Disease Research of China</named-content></funding-source><award-id>2016ZX10004001-003</award-id></award-group></funding-group><counts><page-count count="12"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Niu, Peihua" sort="Niu, Peihua" uniqKey="Niu P" first="Peihua" last="Niu">Peihua Niu</name></noRegion><name sortKey="Deng, Yao" sort="Deng, Yao" uniqKey="Deng Y" first="Yao" last="Deng">Yao Deng</name><name sortKey="Huang, Baoying" sort="Huang, Baoying" uniqKey="Huang B" first="Baoying" last="Huang">Baoying Huang</name><name sortKey="Qin, Kun" sort="Qin, Kun" uniqKey="Qin K" first="Kun" last="Qin">Kun Qin</name><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><name sortKey="Wang, Pengfei" sort="Wang, Pengfei" uniqKey="Wang P" first="Pengfei" last="Wang">Pengfei Wang</name><name sortKey="Wang, Wenling" sort="Wang, Wenling" uniqKey="Wang W" first="Wenling" last="Wang">Wenling Wang</name><name sortKey="Wang, Xinquan" sort="Wang, Xinquan" uniqKey="Wang X" first="Xinquan" last="Wang">Xinquan Wang</name><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name><name sortKey="Zhang, Senyan" sort="Zhang, Senyan" uniqKey="Zhang S" first="Senyan" last="Zhang">Senyan Zhang</name><name sortKey="Zhou, Jianfang" sort="Zhou, Jianfang" uniqKey="Zhou J" first="Jianfang" last="Zhou">Jianfang Zhou</name><name sortKey="Zhou, Panpan" sort="Zhou, Panpan" uniqKey="Zhou P" first="Panpan" last="Zhou">Panpan Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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