A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus
Identifieur interne : 000418 ( Pmc/Checkpoint ); précédent : 000417; suivant : 000419A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus
Auteurs : Yixin Chen [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; Yahong Kang [République populaire de Chine] ; Honglin Chen [République populaire de Chine] ; Hayes Kh Luk [République populaire de Chine] ; Rosana Ws Poon [République populaire de Chine] ; Jasper Fw Chan [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine] ; Ningshao Xia [République populaire de Chine] ; Susanna Kp Lau [République populaire de Chine] ; Patrick Cy Woo [République populaire de Chine]Source :
- Emerging Microbes & Infections [ 2222-1751 ] ; 2019.
Abstract
Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in
Url:
DOI: 10.1038/emi.2015.26
PubMed: 26421268
PubMed Central: 4575394
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus</title><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Luk, Hayes Kh" sort="Luk, Hayes Kh" uniqKey="Luk H" first="Hayes Kh" last="Luk">Hayes Kh Luk</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26421268</idno><idno type="pmc">4575394</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575394</idno><idno type="RBID">PMC:4575394</idno><idno type="doi">10.1038/emi.2015.26</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000C25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C25</idno><idno type="wicri:Area/Pmc/Curation">000C25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C25</idno><idno type="wicri:Area/Pmc/Checkpoint">000418</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000418</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus</title><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Luk, Hayes Kh" sort="Luk, Hayes Kh" uniqKey="Luk H" first="Hayes Kh" last="Luk">Hayes Kh Luk</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Emerging Microbes & Infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in <italic>Escherichia coli</italic>. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID<sub>50</sub>)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID<sub>50</sub>/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ de Groot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hemida, Mg" uniqKey="Hemida M">MG Hemida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B Guery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, X" uniqKey="Gui X">X Gui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, X" uniqKey="Gui X">X Gui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Q" uniqKey="Yuan Q">Q Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapoor, M" uniqKey="Kapoor M">M Kapoor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, Ei" uniqKey="Azhar E">EI Azhar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, Dk" uniqKey="Chu D">DK Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N Nowotny</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alagaili, An" uniqKey="Alagaili A">AN Alagaili</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Microbes Infect</journal-id><journal-id journal-id-type="iso-abbrev">Emerg Microbes Infect</journal-id><journal-id journal-id-type="publisher-id">TEMI</journal-id><journal-id journal-id-type="publisher-id">temi20</journal-id><journal-title-group><journal-title>Emerging Microbes & Infections</journal-title></journal-title-group><issn pub-type="epub">2222-1751</issn><publisher><publisher-name>Taylor & Francis</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26421268</article-id><article-id pub-id-type="pmc">4575394</article-id><article-id pub-id-type="publisher-id">12040307</article-id><article-id pub-id-type="doi">10.1038/emi.2015.26</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Yixin</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>1</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Kwok-Hung</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kang</surname><given-names>Yahong</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>1</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Honglin</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref><xref ref-type="aff" rid="AFF0004"><sup>4</sup></xref><xref ref-type="aff" rid="AFF0005"><sup>5</sup></xref><xref ref-type="aff" rid="AFF0006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Luk</surname><given-names>Hayes KH</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Poon</surname><given-names>Rosana WS</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Jasper FW</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref><xref ref-type="aff" rid="AFF0004"><sup>4</sup></xref><xref ref-type="aff" rid="AFF0005"><sup>5</sup></xref><xref ref-type="aff" rid="AFF0006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yuen</surname><given-names>Kwok-Yung</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref><xref ref-type="aff" rid="AFF0004"><sup>4</sup></xref><xref ref-type="aff" rid="AFF0005"><sup>5</sup></xref><xref ref-type="aff" rid="AFF0006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Xia</surname><given-names>Ningshao</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>1</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lau</surname><given-names>Susanna KP</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref><xref ref-type="aff" rid="AFF0004"><sup>4</sup></xref><xref ref-type="aff" rid="AFF0005"><sup>5</sup></xref><xref ref-type="aff" rid="AFF0006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Woo</surname><given-names>Patrick CY</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>3</sup></xref><xref ref-type="aff" rid="AFF0004"><sup>4</sup></xref><xref ref-type="aff" rid="AFF0005"><sup>5</sup></xref><xref ref-type="aff" rid="AFF0006"><sup>6</sup></xref><xref ref-type="corresp" rid="AN0001"></xref></contrib><aff id="AFF0001"><label>1</label><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></aff><aff id="AFF0002"><label>2</label><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>, Xiamen 361102, Fujian Province,<country>China</country></aff><aff id="AFF0003"><label>3</label><institution>Department of Microbiology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></aff><aff id="AFF0004"><label>4</label><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>, Hong Kong,<country>China</country></aff><aff id="AFF0005"><label>5</label><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>, Hong Kong,<country>China</country></aff><aff id="AFF0006"><label>6</label><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>, Hong Kong,<country>China</country></aff></contrib-group><author-notes><corresp id="AN0001">PCY Woo E-mail: <email>pcywoo@hku.hk</email></corresp></author-notes><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>25</day><month>1</month><year>2019</year></pub-date><volume>4</volume><issue>1</issue><fpage seq="11">1</fpage><lpage>5</lpage><history><date date-type="received"><day>02</day><month>2</month><year>2015</year></date><date date-type="rev-recd"><day>02</day><month>3</month><year>2015</year></date><date date-type="accepted"><day>04</day><month>3</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 The Author(s)</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/"><license-p>This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permissing from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">http://creativecommons.org/licenses/by-nc-sa/3.0/</ext-link></license-p></license><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="TEMI_4_12040307.pdf"></self-uri><abstract id="Abs1"><p>Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in <italic>Escherichia coli</italic>. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID<sub>50</sub>)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID<sub>50</sub>/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.</p></abstract><kwd-group kwd-group-type="author"><title>Keywords</title><kwd>antigen</kwd><kwd>detection</kwd><kwd>ELISA</kwd><kwd>MERS coronavirus</kwd></kwd-group><counts><fig-count count="5"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="37"></ref-count><page-count count="5"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name></noRegion><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name><name sortKey="Luk, Hayes Kh" sort="Luk, Hayes Kh" uniqKey="Luk H" first="Hayes Kh" last="Luk">Hayes Kh Luk</name><name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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