Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection
Identifieur interne : 000201 ( Pmc/Checkpoint ); précédent : 000200; suivant : 000202Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection
Auteurs : Carlton F. O. Hoy [Japon] ; Keiichiro Kushiro [Japon] ; Yutaro Yamaoka [Japon] ; Akihide Ryo [Japon] ; Madoka Takai [Japon]Source :
- Sensing and Bio-Sensing Research [ 2214-1804 ] ; 2019.
Abstract
On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.
Url:
DOI: 10.1016/j.sbsr.2019.100304
PubMed: 32289017
PubMed Central: 7104066
Affiliations:
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Hoy</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Kushiro, Keiichiro" sort="Kushiro, Keiichiro" uniqKey="Kushiro K" first="Keiichiro" last="Kushiro">Keiichiro Kushiro</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Yamaoka, Yutaro" sort="Yamaoka, Yutaro" uniqKey="Yamaoka Y" first="Yutaro" last="Yamaoka">Yutaro Yamaoka</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, School of Medicine, Yokohama City University, Yokohama</wicri:regionArea><wicri:noRegion>Yokohama</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara</wicri:regionArea><wicri:noRegion>Isehara</wicri:noRegion></affiliation></author><author><name sortKey="Ryo, Akihide" sort="Ryo, Akihide" uniqKey="Ryo A" first="Akihide" last="Ryo">Akihide Ryo</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, School of Medicine, Yokohama City University, Yokohama</wicri:regionArea><wicri:noRegion>Yokohama</wicri:noRegion></affiliation></author><author><name sortKey="Takai, Madoka" sort="Takai, Madoka" uniqKey="Takai M" first="Madoka" last="Takai">Madoka Takai</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32289017</idno><idno type="pmc">7104066</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104066</idno><idno type="RBID">PMC:7104066</idno><idno type="doi">10.1016/j.sbsr.2019.100304</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">001143</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001143</idno><idno type="wicri:Area/Pmc/Curation">001143</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001143</idno><idno type="wicri:Area/Pmc/Checkpoint">000201</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000201</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection</title><author><name sortKey="Hoy, Carlton F O" sort="Hoy, Carlton F O" uniqKey="Hoy C" first="Carlton F. O." last="Hoy">Carlton F. O. Hoy</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Kushiro, Keiichiro" sort="Kushiro, Keiichiro" uniqKey="Kushiro K" first="Keiichiro" last="Kushiro">Keiichiro Kushiro</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Yamaoka, Yutaro" sort="Yamaoka, Yutaro" uniqKey="Yamaoka Y" first="Yutaro" last="Yamaoka">Yutaro Yamaoka</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, School of Medicine, Yokohama City University, Yokohama</wicri:regionArea><wicri:noRegion>Yokohama</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara</wicri:regionArea><wicri:noRegion>Isehara</wicri:noRegion></affiliation></author><author><name sortKey="Ryo, Akihide" sort="Ryo, Akihide" uniqKey="Ryo A" first="Akihide" last="Ryo">Akihide Ryo</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, School of Medicine, Yokohama City University, Yokohama</wicri:regionArea><wicri:noRegion>Yokohama</wicri:noRegion></affiliation></author><author><name sortKey="Takai, Madoka" sort="Takai, Madoka" uniqKey="Takai M" first="Madoka" last="Takai">Madoka Takai</name><affiliation wicri:level="4"><nlm:aff id="aff1">Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author></analytic><series><title level="j">Sensing and Bio-Sensing Research</title><idno type="eISSN">2214-1804</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Wilkins, J" uniqKey="Wilkins J">J. Wilkins</name></author><author><name sortKey="Gallimore, J R" uniqKey="Gallimore J">J.R. Gallimore</name></author><author><name sortKey="Moore, E G" uniqKey="Moore E">E.G. Moore</name></author><author><name sortKey="Pepys, M B" uniqKey="Pepys M">M.B. Pepys</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chon, H" uniqKey="Chon H">H. Chon</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sens Biosensing Res</journal-id><journal-id journal-id-type="iso-abbrev">Sens Biosensing Res</journal-id><journal-title-group><journal-title>Sensing and Bio-Sensing Research</journal-title></journal-title-group><issn pub-type="epub">2214-1804</issn><publisher><publisher-name>Elsevier B.V</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32289017</article-id><article-id pub-id-type="pmc">7104066</article-id><article-id pub-id-type="publisher-id">S2214-1804(19)30142-4</article-id><article-id pub-id-type="doi">10.1016/j.sbsr.2019.100304</article-id><article-id pub-id-type="publisher-id">100304</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Hoy</surname><given-names>Carlton F.O.</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Kushiro</surname><given-names>Keiichiro</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Yamaoka</surname><given-names>Yutaro</given-names></name><xref rid="aff2" ref-type="aff">b</xref><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Ryo</surname><given-names>Akihide</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>Takai</surname><given-names>Madoka</given-names></name><email>takai@bis.t.u-tokyo.ac.jp</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">∗</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan</aff><aff id="aff2"><label>b</label>Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan</aff><aff id="aff3"><label>c</label>Isehara Research Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara, Japan</aff><author-notes><corresp id="cor1"><label>∗</label>Corresponding author. <email>takai@bis.t.u-tokyo.ac.jp</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>14</day><month>10</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>11</month><year>2019</year></pub-date><pub-date pub-type="epub"><day>14</day><month>10</month><year>2019</year></pub-date><volume>26</volume><fpage>100304</fpage><lpage>100304</lpage><history><date date-type="received"><day>20</day><month>8</month><year>2019</year></date><date date-type="rev-recd"><day>2</day><month>10</month><year>2019</year></date><date date-type="accepted"><day>3</day><month>10</month><year>2019</year></date></history><permissions><copyright-statement>© 2019 The Authors</copyright-statement><copyright-year>2019</copyright-year><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs0010"><p>On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.</p></abstract><abstract abstract-type="author-highlights" id="abs0015"><title>Highlights</title><p><list list-type="simple" id="ulist0010"><list-item id="u0010"><label>•</label><p id="p0010">3D electrospun polystyrene microfibers were successfully patterned with O<sub>2</sub> plasma.</p></list-item><list-item id="u0015"><label>•</label><p id="p0015">Antigen adsorption were controlled via plasma-treatment time and blocking reagents.</p></list-item><list-item id="u0020"><label>•</label><p id="p0020">Above multiplex microfiber mats could capture multiple antibodies simultaneously.</p></list-item></list></p></abstract><kwd-group id="kwrds0010"><title>Keywords</title><kwd>MERS</kwd><kwd>Multiplex immunoassay</kwd><kwd>Microfiber</kwd><kwd>POC</kwd><kwd>Rapid</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Hoy, Carlton F O" sort="Hoy, Carlton F O" uniqKey="Hoy C" first="Carlton F. O." last="Hoy">Carlton F. O. Hoy</name></region><name sortKey="Kushiro, Keiichiro" sort="Kushiro, Keiichiro" uniqKey="Kushiro K" first="Keiichiro" last="Kushiro">Keiichiro Kushiro</name><name sortKey="Ryo, Akihide" sort="Ryo, Akihide" uniqKey="Ryo A" first="Akihide" last="Ryo">Akihide Ryo</name><name sortKey="Takai, Madoka" sort="Takai, Madoka" uniqKey="Takai M" first="Madoka" last="Takai">Madoka Takai</name><name sortKey="Yamaoka, Yutaro" sort="Yamaoka, Yutaro" uniqKey="Yamaoka Y" first="Yutaro" last="Yamaoka">Yutaro Yamaoka</name><name sortKey="Yamaoka, Yutaro" sort="Yamaoka, Yutaro" uniqKey="Yamaoka Y" first="Yutaro" last="Yamaoka">Yutaro Yamaoka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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