Spike proteins of novel MERS-coronavirus isolates from North- and West-African dromedary camels mediate robust viral entry into human target cells
Identifieur interne : 000178 ( Pmc/Checkpoint ); précédent : 000177; suivant : 000179Spike proteins of novel MERS-coronavirus isolates from North- and West-African dromedary camels mediate robust viral entry into human target cells
Auteurs : Hannah Kleine-Weber [Allemagne] ; Stefan Pöhlmann [Allemagne] ; Markus Hoffmann [Allemagne]Source :
- Virology [ 0042-6822 ] ; 2019.
Abstract
The highly pathogenic Middle East respiratory syndrome (MERS)-related coronavirus (CoV) is transmitted from dromedary camels, the natural reservoir, to humans. For at present unclear reasons, MERS cases have so far only been observed in the Arabian Peninsula, although MERS-CoV also circulates in African dromedary camels. A recent study showed that MERS-CoV found in North/West- (Morocco) and West-African (Burkina Faso and Nigeria) dromedary camels are genetically distinct from Arabian viruses and have reduced replicative capacity in human cells, potentially due to amino acid changes in one or more viral proteins. Here, we show that the spike (S) proteins of the prototypic Arabian MERS-CoV strain, human betacoronavirus 2c EMC/2012, and the above stated African MERS-CoV variants do not appreciably differ in expression, DPP4 binding and ability to drive entry into target cells. Thus, virus-host-interactions at the entry stage may not limit spread of North- and West-African MERS-CoV in human cells.
Url:
DOI: 10.1016/j.virol.2019.07.016
PubMed: 31357164
PubMed Central: 7112047
Affiliations:
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Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2">Faculty of Biology and Psychology, University Göttingen, Wilhelm-Weber-Str. 2, 37073 Göttingen, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Faculty of Biology and Psychology, University Göttingen, Wilhelm-Weber-Str. 2, 37073 Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><affiliation wicri:level="3"><nlm:aff id="aff1">Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2">Faculty of Biology and Psychology, University Göttingen, Wilhelm-Weber-Str. 2, 37073 Göttingen, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Faculty of Biology and Psychology, University Göttingen, Wilhelm-Weber-Str. 2, 37073 Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="Hoffmann, Markus" sort="Hoffmann, Markus" uniqKey="Hoffmann M" first="Markus" last="Hoffmann">Markus Hoffmann</name><affiliation wicri:level="3"><nlm:aff id="aff1">Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><idno type="eISSN">1096-0341</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The highly pathogenic Middle East respiratory syndrome (MERS)-related coronavirus (CoV) is transmitted from dromedary camels, the natural reservoir, to humans. For at present unclear reasons, MERS cases have so far only been observed in the Arabian Peninsula, although MERS-CoV also circulates in African dromedary camels. A recent study showed that MERS-CoV found in North/West- (Morocco) and West-African (Burkina Faso and Nigeria) dromedary camels are genetically distinct from Arabian viruses and have reduced replicative capacity in human cells, potentially due to amino acid changes in one or more viral proteins. Here, we show that the spike (S) proteins of the prototypic Arabian MERS-CoV strain, human betacoronavirus 2c EMC/2012, and the above stated African MERS-CoV variants do not appreciably differ in expression, DPP4 binding and ability to drive entry into target cells. Thus, virus-host-interactions at the entry stage may not limit spread of North- and West-African MERS-CoV in human cells.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ali, M" uniqKey="Ali M">M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Virology</journal-id><journal-id journal-id-type="iso-abbrev">Virology</journal-id><journal-title-group><journal-title>Virology</journal-title></journal-title-group><issn pub-type="ppub">0042-6822</issn><issn pub-type="epub">1096-0341</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31357164</article-id><article-id pub-id-type="pmc">7112047</article-id><article-id pub-id-type="publisher-id">S0042-6822(19)30191-6</article-id><article-id pub-id-type="doi">10.1016/j.virol.2019.07.016</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Spike proteins of novel MERS-coronavirus isolates from North- and West-African dromedary camels mediate robust viral entry into human target cells</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Kleine-Weber</surname><given-names>Hannah</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Pöhlmann</surname><given-names>Stefan</given-names></name><email>spoehlmann@dpz.eu</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref><xref rid="cor1" ref-type="corresp">*</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Hoffmann</surname><given-names>Markus</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany</aff><aff id="aff2"><label>b</label>Faculty of Biology and Psychology, University Göttingen, Wilhelm-Weber-Str. 2, 37073 Göttingen, Germany</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Infection Biology Unit, Deutsches Primatenzentrum - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany. <email>spoehlmann@dpz.eu</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>19</day><month>7</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2019</year></pub-date><pub-date pub-type="epub"><day>19</day><month>7</month><year>2019</year></pub-date><volume>535</volume><fpage>261</fpage><lpage>265</lpage><history><date date-type="received"><day>14</day><month>6</month><year>2019</year></date><date date-type="rev-recd"><day>15</day><month>7</month><year>2019</year></date><date date-type="accepted"><day>18</day><month>7</month><year>2019</year></date></history><permissions><copyright-statement>© 2019 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2019</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs0010"><p>The highly pathogenic Middle East respiratory syndrome (MERS)-related coronavirus (CoV) is transmitted from dromedary camels, the natural reservoir, to humans. For at present unclear reasons, MERS cases have so far only been observed in the Arabian Peninsula, although MERS-CoV also circulates in African dromedary camels. A recent study showed that MERS-CoV found in North/West- (Morocco) and West-African (Burkina Faso and Nigeria) dromedary camels are genetically distinct from Arabian viruses and have reduced replicative capacity in human cells, potentially due to amino acid changes in one or more viral proteins. Here, we show that the spike (S) proteins of the prototypic Arabian MERS-CoV strain, human betacoronavirus 2c EMC/2012, and the above stated African MERS-CoV variants do not appreciably differ in expression, DPP4 binding and ability to drive entry into target cells. Thus, virus-host-interactions at the entry stage may not limit spread of North- and West-African MERS-CoV in human cells.</p></abstract><kwd-group id="kwrds0010"><title>Keywords</title><kwd>MERS-coronavirus</kwd><kwd>Spike</kwd><kwd>Entry</kwd><kwd>Dromedary camel</kwd><kwd>Zoonosis</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Göttingen</li></settlement></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Kleine Weber, Hannah" sort="Kleine Weber, Hannah" uniqKey="Kleine Weber H" first="Hannah" last="Kleine-Weber">Hannah Kleine-Weber</name></region><name sortKey="Hoffmann, Markus" sort="Hoffmann, Markus" uniqKey="Hoffmann M" first="Markus" last="Hoffmann">Markus Hoffmann</name><name sortKey="Kleine Weber, Hannah" sort="Kleine Weber, Hannah" uniqKey="Kleine Weber H" first="Hannah" last="Kleine-Weber">Hannah Kleine-Weber</name><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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