Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
Identifieur interne : 000169 ( Pmc/Checkpoint ); précédent : 000168; suivant : 000170Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
Auteurs : Young-Jun Park [États-Unis] ; Alexandra C. Walls [États-Unis] ; Zhaoqian Wang [États-Unis] ; Maximillian M. Sauer [États-Unis] ; Wentao Li [Pays-Bas] ; M. Alejandra Tortorici [États-Unis, France] ; Berend-Jan Bosch [Pays-Bas] ; Frank Dimaio [États-Unis] ; David Veesler [États-Unis]Source :
- Nature Structural & Molecular Biology [ 1545-9993 ] ; 2019.
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-
Url:
DOI: 10.1038/s41594-019-0334-7
PubMed: 31792450
PubMed Central: 7097669
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Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-<italic>N</italic>-acetyl neuraminic acid, 5-<italic>N</italic>-glycolyl neuraminic acid, sialyl-Lewis<sup>X</sup>, α2,3-sialyl-<italic>N</italic>-acetyl-lactosamine and α2,6-sialyl-<italic>N</italic>-acetyl-lactosamine at 2.7–3.0 Å resolution. We show that recognition occurs via a conserved groove that is essential for MERS-CoV S-mediated attachment to sialosides and entry into human airway epithelial cells. Our data illuminate MERS-CoV S sialoside specificity and suggest that selectivity for α2,3-linked over α2,6-linked receptors results from enhanced interactions with the former class of oligosaccharides. This study provides a structural framework explaining MERS-CoV attachment to sialoside receptors and identifies a site of potential vulnerability to inhibitors of viral entry.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sabir, Js" uniqKey="Sabir J">JS Sabir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, M" uniqKey="Gui M">M Gui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, Rn" uniqKey="Kirchdoerfer R">RN Kirchdoerfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shang, J" uniqKey="Shang J">J Shang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shang, J" uniqKey="Shang J">J Shang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, Rn" uniqKey="Kirchdoerfer R">RN Kirchdoerfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, W" uniqKey="Song W">W Song</name></author><author><name sortKey="Gui, M" uniqKey="Gui M">M Gui</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Xiang, Y" uniqKey="Xiang Y">Y Xiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tortorici, Ma" uniqKey="Tortorici M">MA Tortorici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pallesen, J" uniqKey="Pallesen J">J Pallesen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Je" uniqKey="Park J">JE Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name></author><author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Earnest, Jt" uniqKey="Earnest J">JT Earnest</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Earnest, Jt" uniqKey="Earnest J">JT Earnest</name></author><author><name sortKey="Hantak, Mp" uniqKey="Hantak M">MP Hantak</name></author><author><name sortKey="Park, Je" uniqKey="Park J">JE Park</name></author><author><name sortKey="Gallagher, T" uniqKey="Gallagher T">T Gallagher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stencel Baerenwald, Je" uniqKey="Stencel Baerenwald J">JE Stencel-Baerenwald</name></author><author><name sortKey="Reiss, K" uniqKey="Reiss K">K Reiss</name></author><author><name sortKey="Reiter, Dm" uniqKey="Reiter D">DM Reiter</name></author><author><name sortKey="Stehle, T" uniqKey="Stehle T">T Stehle</name></author><author><name sortKey="Dermody, Ts" uniqKey="Dermody T">TS Dermody</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widagdo, W" uniqKey="Widagdo W">W Widagdo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vergara Alert, J" uniqKey="Vergara Alert J">J Vergara-Alert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barlan, A" uniqKey="Barlan A">A Barlan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulswit, Rjg" uniqKey="Hulswit R">RJG Hulswit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campbell, Mg" uniqKey="Campbell M">MG Campbell</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D Veesler</name></author><author><name sortKey="Cheng, A" uniqKey="Cheng A">A Cheng</name></author><author><name sortKey="Potter, Cs" uniqKey="Potter C">CS Potter</name></author><author><name sortKey="Carragher, B" uniqKey="Carragher B">B Carragher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suzuki, Y" uniqKey="Suzuki Y">Y Suzuki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name></author><author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wickramasinghe, In" uniqKey="Wickramasinghe I">IN Wickramasinghe</name></author><author><name sortKey="De Vries, Rp" uniqKey="De Vries R">RP de Vries</name></author><author><name sortKey="Grone, A" uniqKey="Grone A">A Grone</name></author><author><name sortKey="De Haan, Ca" uniqKey="De Haan C">CA de Haan</name></author><author><name sortKey="Verheije, Mh" uniqKey="Verheije M">MH Verheije</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bakkers, Mj" uniqKey="Bakkers M">MJ Bakkers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Cm" uniqKey="Chan C">CM Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, H" uniqKey="Chu H">H Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widjaja, I" uniqKey="Widjaja I">I Widjaja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suloway, C" uniqKey="Suloway C">C Suloway</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tegunov, D" uniqKey="Tegunov D">D Tegunov</name></author><author><name sortKey="Cramer, P" uniqKey="Cramer P">P Cramer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zivanov, J" uniqKey="Zivanov J">J Zivanov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Punjani, A" uniqKey="Punjani A">A Punjani</name></author><author><name sortKey="Rubinstein, Jl" uniqKey="Rubinstein J">JL Rubinstein</name></author><author><name sortKey="Fleet, Dj" uniqKey="Fleet D">DJ Fleet</name></author><author><name sortKey="Brubaker, Ma" uniqKey="Brubaker M">MA Brubaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zivanov, J" uniqKey="Zivanov J">J Zivanov</name></author><author><name sortKey="Nakane, T" uniqKey="Nakane T">T Nakane</name></author><author><name sortKey="Scheres, Shw" uniqKey="Scheres S">SHW Scheres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scheres, Sh" uniqKey="Scheres S">SH Scheres</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author><author><name sortKey="Huang, Cc" uniqKey="Huang C">CC Huang</name></author><author><name sortKey="Ferrin, Te" uniqKey="Ferrin T">TE Ferrin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brown, A" uniqKey="Brown A">A Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P Emsley</name></author><author><name sortKey="Lohkamp, B" uniqKey="Lohkamp B">B Lohkamp</name></author><author><name sortKey="Scott, Wg" uniqKey="Scott W">WG Scott</name></author><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author><author><name sortKey="Leaver Fay, A" uniqKey="Leaver Fay A">A Leaver-Fay</name></author><author><name sortKey="Bradley, P" uniqKey="Bradley P">P Bradley</name></author><author><name sortKey="Baker, D" uniqKey="Baker D">D Baker</name></author><author><name sortKey="Andre, I" uniqKey="Andre I">I Andre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frenz, B" uniqKey="Frenz B">B Frenz</name></author><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author><author><name sortKey="Egelman, Eh" uniqKey="Egelman E">EH Egelman</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D Veesler</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frenz, Bdr" uniqKey="Frenz B">BDR Frenz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A" uniqKey="Walls A">A Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Ry" uniqKey="Wang R">RY Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Vb" uniqKey="Chen V">VB Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barad, Ba" uniqKey="Barad B">BA Barad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agirre, J" uniqKey="Agirre J">J Agirre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krissinel, E" uniqKey="Krissinel E">E Krissinel</name></author><author><name sortKey="Henrick, K" uniqKey="Henrick K">K Henrick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Nat Struct Mol Biol</journal-id><journal-id journal-id-type="iso-abbrev">Nat. Struct. Mol. Biol</journal-id><journal-title-group><journal-title>Nature Structural & Molecular Biology</journal-title></journal-title-group><issn pub-type="ppub">1545-9993</issn><issn pub-type="epub">1545-9985</issn><publisher><publisher-name>Nature Publishing Group US</publisher-name><publisher-loc>New York</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31792450</article-id><article-id pub-id-type="pmc">7097669</article-id><article-id pub-id-type="publisher-id">334</article-id><article-id pub-id-type="doi">10.1038/s41594-019-0334-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Park</surname><given-names>Young-Jun</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Walls</surname><given-names>Alexandra C.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Wang</surname><given-names>Zhaoqian</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Sauer</surname><given-names>Maximillian M.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Wentao</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-2260-2577</contrib-id><name><surname>Tortorici</surname><given-names>M. Alejandra</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Bosch</surname><given-names>Berend-Jan</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>DiMaio</surname><given-names>Frank</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-6019-8675</contrib-id><name><surname>Veesler</surname><given-names>David</given-names></name><address><email>dveesler@uw.edu</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine,</institution><institution>Utrecht University,</institution></institution-wrap>Utrecht, the Netherlands</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>Institut Pasteur, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>CNRS UMR 3569, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</aff></contrib-group><pub-date pub-type="epub"><day>2</day><month>12</month><year>2019</year></pub-date><pub-date pub-type="ppub"><year>2019</year></pub-date><volume>26</volume><issue>12</issue><fpage>1151</fpage><lpage>1157</lpage><history><date date-type="received"><day>19</day><month>7</month><year>2019</year></date><date date-type="accepted"><day>21</day><month>10</month><year>2019</year></date></history><permissions><copyright-statement>© The Author(s), under exclusive licence to Springer Nature America, Inc. 2019</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-<italic>N</italic>-acetyl neuraminic acid, 5-<italic>N</italic>-glycolyl neuraminic acid, sialyl-Lewis<sup>X</sup>, α2,3-sialyl-<italic>N</italic>-acetyl-lactosamine and α2,6-sialyl-<italic>N</italic>-acetyl-lactosamine at 2.7–3.0 Å resolution. We show that recognition occurs via a conserved groove that is essential for MERS-CoV S-mediated attachment to sialosides and entry into human airway epithelial cells. Our data illuminate MERS-CoV S sialoside specificity and suggest that selectivity for α2,3-linked over α2,6-linked receptors results from enhanced interactions with the former class of oligosaccharides. This study provides a structural framework explaining MERS-CoV attachment to sialoside receptors and identifies a site of potential vulnerability to inhibitors of viral entry.</p></abstract><abstract id="Abs2" abstract-type="web-summary"><p id="Par2">Cryo-EM structures of MERS-CoV S glycoprotein trimer in complex with different sialosides reveal how the virus engages with sialylated receptors, providing insight into receptor specificity and selectivity.</p></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>Cryoelectron microscopy</kwd><kwd>Viral membrane fusion</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s), under exclusive licence to Springer Nature America, Inc. 2019</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>France</li><li>Pays-Bas</li><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Park, Young Jun" sort="Park, Young Jun" uniqKey="Park Y" first="Young-Jun" last="Park">Young-Jun Park</name></region><name sortKey="Dimaio, Frank" sort="Dimaio, Frank" uniqKey="Dimaio F" first="Frank" last="Dimaio">Frank Dimaio</name><name sortKey="Sauer, Maximillian M" sort="Sauer, Maximillian M" uniqKey="Sauer M" first="Maximillian M." last="Sauer">Maximillian M. Sauer</name><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name><name sortKey="Veesler, David" sort="Veesler, David" uniqKey="Veesler D" first="David" last="Veesler">David Veesler</name><name sortKey="Walls, Alexandra C" sort="Walls, Alexandra C" uniqKey="Walls A" first="Alexandra C." last="Walls">Alexandra C. Walls</name><name sortKey="Wang, Zhaoqian" sort="Wang, Zhaoqian" uniqKey="Wang Z" first="Zhaoqian" last="Wang">Zhaoqian Wang</name></country><country name="Pays-Bas"><noRegion><name sortKey="Li, Wentao" sort="Li, Wentao" uniqKey="Li W" first="Wentao" last="Li">Wentao Li</name></noRegion><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name></country><country name="France"><noRegion><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name></noRegion><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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