MersV1, PascalFrancis, Curation, bibRecord, 000115

Serological detection of attenuated HIV-1 variants with nef gene deletions

Identifieur interne : 000115 ( PascalFrancis/Curation ); précédent : 000114; suivant : 000116

Serological detection of attenuated HIV-1 variants with nef gene deletions

Auteurs : A. L. Greenway [Australie] ; J. Mills [Australie] ; D. Rhodes [Australie] ; N. J. Deacon [Australie] ; D. A. Mcphee [Australie]

Source :

AIDS : (London) [ 0269-9370 ] ; 1998.

RBID : Pascal:98-0256362

Descripteurs français

Pascal (Inist)
- SIDA, Virus HIV1, Diagnostic, Sérologie, Délétion, Gène, Homme, Australie.
Wicri :
- geographic : Australie.
- topic : Homme.

English descriptors

KwdEn :
- AIDS, Australia, Deletion, Diagnosis, Gene, HIV-1 virus, Human, Serology.

Abstract

Objective: To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with a net-deleted strain of HIV-1 could be differentiated from individuals infected with wild-type strains of HIV-1 by characterizing the Nef antibody response of cohort members. Design: Retrospective and prospective analysis of the nef gene sequence and the antibody response to Nef peptides in HIV-infected subjects. Methods: Plasma was obtained from all individuals of the Sydney cohort, and from a variety of HlV-1-infected and uninfected controls. Antibodies recognizing full-length recombinant HIV-1_NL43 Nef protein and synthetic peptide analogues were assessed by enzyme-linked immunosorbent assay. Results: All 34 individuals infected with wild-type HIV-1 had antibodies reacting with full-length Nef protein as well as with a series of synthetic peptides (6-23-mers) spanning most of the Nef protein of HIV-1_NL43. Although the HIV-1 quasispecies infecting the Sydney cohort had a consensus deletion of the nef gene corresponding to amino-acids 165-206, HIV-1 strains from individual members of the cohort had additional deletions comprising up to 80% of the nef gene. Members of the cohort had antibodies to peptides homologous to all regions of the Nef protein tested, except for a single peptide (amino-acids 162-177) that lies within the consensus nef deletion for the cohort quasispecies. Conclusion: These data show that nef-deleted strains of HIV-1 can be detected serologically. In the Sydney cohort, detection of antibodies to all regions of Nef tested, except that corresponding to amino-acids 162-177, suggests that observed deletions outside this domain occurred after this virus had infected these subjects and stimulated an immune response. A Nef peptide serological assay may be useful for identifying further examples of individuals infected with nef-deleted, attenuated HIV-1 quasispecies and for assessing the evolution of those variants in vivo.

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A03		`1`		`@0 AIDS : (Lond.)`
A05				`@2 12`
A06				`@2 6`
A08	`01`	`1`	`ENG`	`@1 Serological detection of attenuated HIV-1 variants with nef gene deletions`
A11	`01`	`1`		`@1 GREENWAY (A. L.)`
A11	`02`	`1`		`@1 MILLS (J.)`
A11	`03`	`1`		`@1 RHODES (D.)`
A11	`04`	`1`		`@1 DEACON (N. J.)`
A11	`05`	`1`		`@1 MCPHEE (D. A.)`
A14	`01`			`@1 AIDS Cellular Biology Unit, National Centre in HIV Virology Research, Macfarlane Burnet Centre for Medical Research @2 Fairfield, Victoria @3 AUS @Z 1 aut. @Z 5 aut.`
A14	`02`			`@1 National Centre in HIV Virology Research, National Centre in HIV Virology Research, Macfarlane Burnet Centre for Medical Research @2 Fairfield, Victoria @3 AUS @Z 2 aut. @Z 4 aut. @Z 5 aut.`
A14	`03`			`@1 AIDS Molecular Biology Unit, National Centre in HIV Virology Research, Macfarlane Burnet Centre for Medical Research @2 Fairfield, Victoria @3 AUS @Z 3 aut.`
A20				`@1 555-561`
A21				`@1 1998`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 22094 @5 354000075431440030`
A44				`@0 0000 @1 © 1998 INIST-CNRS. All rights reserved.`
A45				`@0 24 ref.`
A47	`01`	`1`		`@0 98-0256362`
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A64		`1`		`@0 AIDS : (London)`
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C01	`01`		`ENG`	@0 Objective: To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with a net-deleted strain of HIV-1 could be differentiated from individuals infected with wild-type strains of HIV-1 by characterizing the Nef antibody response of cohort members. Design: Retrospective and prospective analysis of the nef gene sequence and the antibody response to Nef peptides in HIV-infected subjects. Methods: Plasma was obtained from all individuals of the Sydney cohort, and from a variety of HlV-1-infected and uninfected controls. Antibodies recognizing full-length recombinant HIV-1_NL43 Nef protein and synthetic peptide analogues were assessed by enzyme-linked immunosorbent assay. Results: All 34 individuals infected with wild-type HIV-1 had antibodies reacting with full-length Nef protein as well as with a series of synthetic peptides (6-23-mers) spanning most of the Nef protein of HIV-1_NL43. Although the HIV-1 quasispecies infecting the Sydney cohort had a consensus deletion of the nef gene corresponding to amino-acids 165-206, HIV-1 strains from individual members of the cohort had additional deletions comprising up to 80% of the nef gene. Members of the cohort had antibodies to peptides homologous to all regions of the Nef protein tested, except for a single peptide (amino-acids 162-177) that lies within the consensus nef deletion for the cohort quasispecies. Conclusion: These data show that nef-deleted strains of HIV-1 can be detected serologically. In the Sydney cohort, detection of antibodies to all regions of Nef tested, except that corresponding to amino-acids 162-177, suggests that observed deletions outside this domain occurred after this virus had infected these subjects and stimulated an immune response. A Nef peptide serological assay may be useful for identifying further examples of individuals infected with nef-deleted, attenuated HIV-1 quasispecies and for assessing the evolution of those variants in vivo.
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C03	`05`	`X`	`FRE`	`@0 Délétion @5 05`
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C03	`05`	`X`	`SPA`	`@0 Deleción @5 05`
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C07	`03`	`X`	`FRE`	`@0 Virus immunodéficience humaine @2 NW`
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C07	`05`	`X`	`FRE`	`@0 Retroviridae @2 NW`
C07	`05`	`X`	`ENG`	`@0 Retroviridae @2 NW`
C07	`05`	`X`	`SPA`	`@0 Retroviridae @2 NW`
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C07	`07`	`X`	`FRE`	`@0 Océanie @2 NG`
C07	`07`	`X`	`ENG`	`@0 Oceania @2 NG`
C07	`07`	`X`	`SPA`	`@0 Oceania @2 NG`
C07	`08`	`X`	`FRE`	`@0 Immunopathologie @5 37`
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C07	`09`	`X`	`SPA`	`@0 Inmunodeficiencia @5 38`
N21				`@1 166`

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Le document en format XML

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<author><name sortKey="Mills, J" sort="Mills, J" uniqKey="Mills J" first="J." last="Mills">J. Mills</name>
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<s2>Fairfield, Victoria</s2>
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<author><name sortKey="Rhodes, D" sort="Rhodes, D" uniqKey="Rhodes D" first="D." last="Rhodes">D. Rhodes</name>
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<term>Human</term>
<term>Serology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>SIDA</term>
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<front><div type="abstract" xml:lang="en">Objective: To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with a net-deleted strain of HIV-1 could be differentiated from individuals infected with wild-type strains of HIV-1 by characterizing the Nef antibody response of cohort members. Design: Retrospective and prospective analysis of the nef gene sequence and the antibody response to Nef peptides in HIV-infected subjects. Methods: Plasma was obtained from all individuals of the Sydney cohort, and from a variety of HlV-1-infected and uninfected controls. Antibodies recognizing full-length recombinant HIV-1<sub>NL43</sub>
 Nef protein and synthetic peptide analogues were assessed by enzyme-linked immunosorbent assay. Results: All 34 individuals infected with wild-type HIV-1 had antibodies reacting with full-length Nef protein as well as with a series of synthetic peptides (6-23-mers) spanning most of the Nef protein of HIV-1<sub>NL43</sub>
. Although the HIV-1 quasispecies infecting the Sydney cohort had a consensus deletion of the nef gene corresponding to amino-acids 165-206, HIV-1 strains from individual members of the cohort had additional deletions comprising up to 80% of the nef gene. Members of the cohort had antibodies to peptides homologous to all regions of the Nef protein tested, except for a single peptide (amino-acids 162-177) that lies within the consensus nef deletion for the cohort quasispecies. Conclusion: These data show that nef-deleted strains of HIV-1 can be detected serologically. In the Sydney cohort, detection of antibodies to all regions of Nef tested, except that corresponding to amino-acids 162-177, suggests that observed deletions outside this domain occurred after this virus had infected these subjects and stimulated an immune response. A Nef peptide serological assay may be useful for identifying further examples of individuals infected with nef-deleted, attenuated HIV-1 quasispecies and for assessing the evolution of those variants in vivo.</div>
</front>
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<sZ>1 aut.</sZ>
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<sZ>2 aut.</sZ>
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<fA14 i1="03"><s1>AIDS Molecular Biology Unit, National Centre in HIV Virology Research, Macfarlane Burnet Centre for Medical Research</s1>
<s2>Fairfield, Victoria</s2>
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<fC01 i1="01" l="ENG"><s0>Objective: To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with a net-deleted strain of HIV-1 could be differentiated from individuals infected with wild-type strains of HIV-1 by characterizing the Nef antibody response of cohort members. Design: Retrospective and prospective analysis of the nef gene sequence and the antibody response to Nef peptides in HIV-infected subjects. Methods: Plasma was obtained from all individuals of the Sydney cohort, and from a variety of HlV-1-infected and uninfected controls. Antibodies recognizing full-length recombinant HIV-1<sub>NL43</sub>
 Nef protein and synthetic peptide analogues were assessed by enzyme-linked immunosorbent assay. Results: All 34 individuals infected with wild-type HIV-1 had antibodies reacting with full-length Nef protein as well as with a series of synthetic peptides (6-23-mers) spanning most of the Nef protein of HIV-1<sub>NL43</sub>
. Although the HIV-1 quasispecies infecting the Sydney cohort had a consensus deletion of the nef gene corresponding to amino-acids 165-206, HIV-1 strains from individual members of the cohort had additional deletions comprising up to 80% of the nef gene. Members of the cohort had antibodies to peptides homologous to all regions of the Nef protein tested, except for a single peptide (amino-acids 162-177) that lies within the consensus nef deletion for the cohort quasispecies. Conclusion: These data show that nef-deleted strains of HIV-1 can be detected serologically. In the Sydney cohort, detection of antibodies to all regions of Nef tested, except that corresponding to amino-acids 162-177, suggests that observed deletions outside this domain occurred after this virus had infected these subjects and stimulated an immune response. A Nef peptide serological assay may be useful for identifying further examples of individuals infected with nef-deleted, attenuated HIV-1 quasispecies and for assessing the evolution of those variants in vivo.</s0>
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<fC07 i1="06" i2="X" l="SPA"><s0>Virus</s0>
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<s2>NG</s2>
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<fC07 i1="09" i2="X" l="SPA"><s0>Inmunodeficiencia</s0>
<s5>38</s5>
</fC07>
<fN21><s1>166</s1>
</fN21>
</pA>
</standard>
</inist>
</record>

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   |wiki=    Sante
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   |texte=   Serological detection of attenuated HIV-1 variants with nef gene deletions
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Serological detection of attenuated HIV-1 variants with nef gene deletions

Serological detection of attenuated HIV-1 variants with nef gene deletions

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