Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B
Identifieur interne : 000025 ( PascalFrancis/Curation ); précédent : 000024; suivant : 000026Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B
Auteurs : Aziz Alami Chentoufi [États-Unis] ; Nicholas R. Binder [États-Unis] ; Noureddine Berka [Canada] ; Guillaume Durand [France] ; Alex Nguyen [États-Unis] ; Ilham Bettahi [États-Unis] ; Bernard Maillere [France] ; Lbachir Benmohamed [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2008.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, a library of 179 potential peptide epitopes (15-mers overlapping by 10 amino acids) was identified from HSV type 1 (HSV-1) glycoprotein B (gB), an antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) of 10 adjacent peptides each were first screened for T-cell antigenicity in 38 HSV-1-seropositive but HSV-2-seronegative individuals. Individual peptides within the two immunodominant groups (i.e., G4 and G14) were further screened with T cells from HLA-DR-genotyped and clinically defined symptomatic (n = 10) and asymptomatic (n = 10) HSV-1-seropositive healthy individuals. Peptides gB161-175 and gB166-180 within G4 and gB661-675 within G14 recalled the strongest HLA-DR-dependent CD4+ T-cell proliferation and gamma interferon production. gB166-180, gB661-675, and gB666-680 elicited ex vivo CD4+ cytotoxic T cells (CTLs) that lysed autologous HSV-1- and vaccinia virus (expressing gB)-infected lymphoblastoid cell lines. Interestingly, gB166-180 and gB666-680 peptide epitopes were strongly recognized by CD4+ T cells from 10 of 10 asymptomatic patients but not by CD4+ T cells from 10 of 10 symptomatic patients (P < 0.0001; analysis of variance posttest). Inversely, CD4+ T cells from symptomatic patients preferentially recognized gB661-675 (P < 0.0001). Thus, we identified three previously unrecognized CD4+ CTL peptide epitopes in HSV-1 gB. Among these, gB166-180 and gB666-680 appear to be "asymptomatic" peptide epitopes and therefore should be considered in the design of future herpes vaccines.
pA |
|
---|
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000069
Links to Exploration step
Pascal:09-0013229Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B</title>
<author><name sortKey="Alami Chentoufi, Aziz" sort="Alami Chentoufi, Aziz" uniqKey="Alami Chentoufi A" first="Aziz" last="Alami Chentoufi">Aziz Alami Chentoufi</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Binder, Nicholas R" sort="Binder, Nicholas R" uniqKey="Binder N" first="Nicholas R." last="Binder">Nicholas R. Binder</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Berka, Noureddine" sort="Berka, Noureddine" uniqKey="Berka N" first="Noureddine" last="Berka">Noureddine Berka</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Tissue Typing Laboratory</s1>
<s2>Calgary, Alberta T2L2K8</s2>
<s3>CAN</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Durand, Guillaume" sort="Durand, Guillaume" uniqKey="Durand G" first="Guillaume" last="Durand">Guillaume Durand</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>CEA, iBiTecS, Service d'Ingenierie Moléculaire des Protéines (SIMOPRO)</s1>
<s2>Gif Sur Yvette 91191</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Nguyen, Alex" sort="Nguyen, Alex" uniqKey="Nguyen A" first="Alex" last="Nguyen">Alex Nguyen</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Bettahi, Ilham" sort="Bettahi, Ilham" uniqKey="Bettahi I" first="Ilham" last="Bettahi">Ilham Bettahi</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Maillere, Bernard" sort="Maillere, Bernard" uniqKey="Maillere B" first="Bernard" last="Maillere">Bernard Maillere</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>CEA, iBiTecS, Service d'Ingenierie Moléculaire des Protéines (SIMOPRO)</s1>
<s2>Gif Sur Yvette 91191</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Benmohamed, Lbachir" sort="Benmohamed, Lbachir" uniqKey="Benmohamed L" first="Lbachir" last="Benmohamed">Lbachir Benmohamed</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Center for Immunology, University of California Irvine</s1>
<s2>Irvine, California 92697-1450</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">09-0013229</idno>
<date when="2008">2008</date>
<idno type="stanalyst">PASCAL 09-0013229 INIST</idno>
<idno type="RBID">Pascal:09-0013229</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000069</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000025</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B</title>
<author><name sortKey="Alami Chentoufi, Aziz" sort="Alami Chentoufi, Aziz" uniqKey="Alami Chentoufi A" first="Aziz" last="Alami Chentoufi">Aziz Alami Chentoufi</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Binder, Nicholas R" sort="Binder, Nicholas R" uniqKey="Binder N" first="Nicholas R." last="Binder">Nicholas R. Binder</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Berka, Noureddine" sort="Berka, Noureddine" uniqKey="Berka N" first="Noureddine" last="Berka">Noureddine Berka</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Tissue Typing Laboratory</s1>
<s2>Calgary, Alberta T2L2K8</s2>
<s3>CAN</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Durand, Guillaume" sort="Durand, Guillaume" uniqKey="Durand G" first="Guillaume" last="Durand">Guillaume Durand</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>CEA, iBiTecS, Service d'Ingenierie Moléculaire des Protéines (SIMOPRO)</s1>
<s2>Gif Sur Yvette 91191</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Nguyen, Alex" sort="Nguyen, Alex" uniqKey="Nguyen A" first="Alex" last="Nguyen">Alex Nguyen</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Bettahi, Ilham" sort="Bettahi, Ilham" uniqKey="Bettahi I" first="Ilham" last="Bettahi">Ilham Bettahi</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Maillere, Bernard" sort="Maillere, Bernard" uniqKey="Maillere B" first="Bernard" last="Maillere">Bernard Maillere</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>CEA, iBiTecS, Service d'Ingenierie Moléculaire des Protéines (SIMOPRO)</s1>
<s2>Gif Sur Yvette 91191</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Benmohamed, Lbachir" sort="Benmohamed, Lbachir" uniqKey="Benmohamed L" first="Lbachir" last="Benmohamed">Lbachir Benmohamed</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Center for Immunology, University of California Irvine</s1>
<s2>Irvine, California 92697-1450</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term>
<term>Asymptomatic</term>
<term>Cytotoxic T lymphocyte</term>
<term>Glycoprotein</term>
<term>Herpesvirus hominis</term>
<term>Human</term>
<term>T-Lymphocyte</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Herpesvirus hominis</term>
<term>Asymptomatique</term>
<term>Lymphocyte T</term>
<term>Lymphocyte T cytotoxique</term>
<term>Déterminant antigénique</term>
<term>Glycoprotéine</term>
<term>Virologie</term>
<term>Antigène CD4</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, a library of 179 potential peptide epitopes (15-mers overlapping by 10 amino acids) was identified from HSV type 1 (HSV-1) glycoprotein B (gB), an antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) of 10 adjacent peptides each were first screened for T-cell antigenicity in 38 HSV-1-seropositive but HSV-2-seronegative individuals. Individual peptides within the two immunodominant groups (i.e., G4 and G14) were further screened with T cells from HLA-DR-genotyped and clinically defined symptomatic (n = 10) and asymptomatic (n = 10) HSV-1-seropositive healthy individuals. Peptides gB<sub>161-175</sub>
and gB<sub>166-180</sub>
within G4 and gB<sub>661-675</sub>
within G14 recalled the strongest HLA-DR-dependent CD4<sup>+</sup>
T-cell proliferation and gamma interferon production. gB<sub>166-180,</sub>
gB<sub>661-675</sub>
, and gB<sub>666-680</sub>
elicited ex vivo CD4<sup>+</sup>
cytotoxic T cells (CTLs) that lysed autologous HSV-1- and vaccinia virus (expressing gB)-infected lymphoblastoid cell lines. Interestingly, gB<sub>166-180</sub>
and gB<sub>666-680</sub>
peptide epitopes were strongly recognized by CD4<sup>+</sup>
T cells from 10 of 10 asymptomatic patients but not by CD4<sup>+</sup>
T cells from 10 of 10 symptomatic patients (P < 0.0001; analysis of variance posttest). Inversely, CD4<sup>+</sup>
T cells from symptomatic patients preferentially recognized gB<sub>661-675</sub>
(P < 0.0001). Thus, we identified three previously unrecognized CD4<sup>+</sup>
CTL peptide epitopes in HSV-1 gB. Among these, gB<sub>166-180</sub>
and gB<sub>666-680</sub>
appear to be "asymptomatic" peptide epitopes and therefore should be considered in the design of future herpes vaccines.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0>
</fA01>
<fA03 i2="1"><s0>J. virol.</s0>
</fA03>
<fA05><s2>82</s2>
</fA05>
<fA06><s2>23</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>ALAMI CHENTOUFI (Aziz)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>BINDER (Nicholas R.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>BERKA (Noureddine)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>DURAND (Guillaume)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>NGUYEN (Alex)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BETTAHI (Ilham)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>MAILLERE (Bernard)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>BENMOHAMED (Lbachir)</s1>
</fA11>
<fA14 i1="01"><s1>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine</s1>
<s2>Irvine, California 92697-4375</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Tissue Typing Laboratory</s1>
<s2>Calgary, Alberta T2L2K8</s2>
<s3>CAN</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>CEA, iBiTecS, Service d'Ingenierie Moléculaire des Protéines (SIMOPRO)</s1>
<s2>Gif Sur Yvette 91191</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Center for Immunology, University of California Irvine</s1>
<s2>Irvine, California 92697-1450</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>11792-11802</s1>
</fA20>
<fA21><s1>2008</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13592</s2>
<s5>354000184531680310</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2009 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>80 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>09-0013229</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, a library of 179 potential peptide epitopes (15-mers overlapping by 10 amino acids) was identified from HSV type 1 (HSV-1) glycoprotein B (gB), an antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) of 10 adjacent peptides each were first screened for T-cell antigenicity in 38 HSV-1-seropositive but HSV-2-seronegative individuals. Individual peptides within the two immunodominant groups (i.e., G4 and G14) were further screened with T cells from HLA-DR-genotyped and clinically defined symptomatic (n = 10) and asymptomatic (n = 10) HSV-1-seropositive healthy individuals. Peptides gB<sub>161-175</sub>
and gB<sub>166-180</sub>
within G4 and gB<sub>661-675</sub>
within G14 recalled the strongest HLA-DR-dependent CD4<sup>+</sup>
T-cell proliferation and gamma interferon production. gB<sub>166-180,</sub>
gB<sub>661-675</sub>
, and gB<sub>666-680</sub>
elicited ex vivo CD4<sup>+</sup>
cytotoxic T cells (CTLs) that lysed autologous HSV-1- and vaccinia virus (expressing gB)-infected lymphoblastoid cell lines. Interestingly, gB<sub>166-180</sub>
and gB<sub>666-680</sub>
peptide epitopes were strongly recognized by CD4<sup>+</sup>
T cells from 10 of 10 asymptomatic patients but not by CD4<sup>+</sup>
T cells from 10 of 10 symptomatic patients (P < 0.0001; analysis of variance posttest). Inversely, CD4<sup>+</sup>
T cells from symptomatic patients preferentially recognized gB<sub>661-675</sub>
(P < 0.0001). Thus, we identified three previously unrecognized CD4<sup>+</sup>
CTL peptide epitopes in HSV-1 gB. Among these, gB<sub>166-180</sub>
and gB<sub>666-680</sub>
appear to be "asymptomatic" peptide epitopes and therefore should be considered in the design of future herpes vaccines.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Homme</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Human</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Hombre</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Herpesvirus hominis</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Herpesvirus hominis</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Herpesvirus hominis</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Asymptomatique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Asymptomatic</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Asintomático</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Lymphocyte T</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>T-Lymphocyte</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Linfocito T</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Lymphocyte T cytotoxique</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Cytotoxic T lymphocyte</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Linfocito T citotóxico</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Déterminant antigénique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Antigenic determinant</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Determinante antigénico</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Glycoprotéine</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Glycoprotein</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Glicoproteína</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Virologie</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Virology</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Virología</s0>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Antigène CD4</s0>
<s4>INC</s4>
<s5>79</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fN21><s1>007</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PascalFrancis/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000025 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000025 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PascalFrancis |étape= Curation |type= RBID |clé= Pascal:09-0013229 |texte= Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B }}
This area was generated with Dilib version V0.6.33. |