Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study
Identifieur interne : 000038 ( PascalFrancis/Corpus ); précédent : 000037; suivant : 000039Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study
Auteurs : Chantal B. E. M. Reusken ; Bart L. Haagmans ; Marcel A. Müller ; Carlos Gutierrez ; Gert-Jan Godeke ; Benjamin Meyer ; Doreen Muth ; V. Stalin Raj ; Laura Smits-De Vries ; Victor M. Corman ; Jan-Felix Drexler ; Saskia L. Smits ; Yasmin E. El Tahir ; Rita De Sousa ; Janko Van Beek ; Norbert Nowotny ; Kees Van Maanen ; Ezequiel Hidalgo-Hermoso ; Berend-Jan Bosch ; Peter Rottier ; Albert Osterhaus ; Christian Gortazar-Schmidt ; Christian Drosten ; Marion P. G. KoopmansSource :
- Lancet. Infectious diseases : (print) [ 1473-3099 ] ; 2013.
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- Pascal (Inist)
English descriptors
Abstract
Background A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.
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Format Inist (serveur)
| NO : | PASCAL 13-0329482 INIST |
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| ET : | Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study |
| AU : | REUSKEN (Chantal B. E. M.); HAAGMANS (Bart L.); MÜLLER (Marcel A.); GUTIERREZ (Carlos); GODEKE (Gert-Jan); MEYER (Benjamin); MUTH (Doreen); STALIN RAJ (V.); SMITS-DE VRIES (Laura); CORMAN (Victor M.); DREXLER (Jan-Felix); SMITS (Saskia L.); EL TAHIR (Yasmin E.); DE SOUSA (Rita); VAN BEEK (Janko); NOWOTNY (Norbert); VAN MAANEN (Kees); HIDALGO-HERMOSO (Ezequiel); BOSCH (Berend-Jan); ROTTIER (Peter); OSTERHAUS (Albert); GORTAZAR-SCHMIDT (Christian); DROSTEN (Christian); KOOPMANS (Marion P. G.) |
| AF : | Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment/Bilthoven/Pays-Bas (1 aut., 5 aut., 14 aut., 15 aut., 24 aut.); Department of Viroscience, Erasmus Medical Centre/Rotterdam/Pays-Bas (2 aut., 8 aut., 12 aut., 21 aut., 24 aut.); Institute of Virology, University of Bonn Medical Centre/Bonn/Allemagne (3 aut., 6 aut., 7 aut., 10 aut., 11 aut., 23 aut.); Department of Animal Medicine and Surgery, Veterinary Faculty, University of Las Palmas de Gran Canaria/Las Palmas, Canary Islands/Espagne (4 aut.); Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine/Utrecht/Pays-Bas (9 aut., 19 aut., 20 aut.); Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University/Muscat/Oman (13 aut.); Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University/Muscat/Oman (16 aut.); The European Programme for Public Health Microbiology Training, European Center for Disease Control/Stockholm/Suède (14 aut.); Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna/Vienna/Autriche (16 aut.); Animal Health Service/Deventer/Pays-Bas (17 aut.); Department of Conservation and Research, Parque Zoologico Buin Zoo/Buin/Chili (18 aut.); SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real/Espagne (22 aut.); Viroclinics Biosciences BV/Rotterdam/Pays-Bas (12 aut., 21 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Lancet. Infectious diseases : (print); ISSN 1473-3099; Royaume-Uni; Da. 2013; Vol. 13; No. 10; Pp. 859-866; Bibl. 38 ref. |
| LA : | Anglais |
| EA : | Background A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft. |
| CC : | 002B05C02C |
| FD : | Sérum; Anticorps; Etude comparative; Coronavirus; Syndrome respiratoire du Moyen-Orient |
| FG : | Coronaviridae; Nidovirales; Virus; Pathologie de l'appareil respiratoire; Virose; Infection |
| ED : | Serum; Antibody; Comparative study; Coronavirus; Middle East respiratory syndrome |
| EG : | Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection |
| SD : | Suero; Anticuerpo; Estudio comparativo; Coronavirus |
| LO : | INIST-27478.354000505197280120 |
| ID : | 13-0329482 |
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Stalin Raj</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Smits De Vries, Laura" sort="Smits De Vries, Laura" uniqKey="Smits De Vries L" first="Laura" last="Smits-De Vries">Laura Smits-De Vries</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan-Felix" last="Drexler">Jan-Felix Drexler</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Smits, Saskia L" sort="Smits, Saskia L" uniqKey="Smits S" first="Saskia L." last="Smits">Saskia L. Smits</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="13"><s1>Viroclinics Biosciences BV</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>12 aut.</sZ><sZ>21 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="El Tahir, Yasmin E" sort="El Tahir, Yasmin E" uniqKey="El Tahir Y" first="Yasmin E." last="El Tahir">Yasmin E. El Tahir</name><affiliation><inist:fA14 i1="06"><s1>Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="De Sousa, Rita" sort="De Sousa, Rita" uniqKey="De Sousa R" first="Rita" last="De Sousa">Rita De Sousa</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="08"><s1>The European Programme for Public Health Microbiology Training, European Center for Disease Control</s1><s2>Stockholm</s2><s3>SWE</s3><sZ>14 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Van Beek, Janko" sort="Van Beek, Janko" uniqKey="Van Beek J" first="Janko" last="Van Beek">Janko Van Beek</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name><affiliation><inist:fA14 i1="07"><s1>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>16 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="09"><s1>Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>16 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Van Maanen, Kees" sort="Van Maanen, Kees" uniqKey="Van Maanen K" first="Kees" last="Van Maanen">Kees Van Maanen</name><affiliation><inist:fA14 i1="10"><s1>Animal Health Service</s1><s2>Deventer</s2><s3>NLD</s3><sZ>17 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Hidalgo Hermoso, Ezequiel" sort="Hidalgo Hermoso, Ezequiel" uniqKey="Hidalgo Hermoso E" first="Ezequiel" last="Hidalgo-Hermoso">Ezequiel Hidalgo-Hermoso</name><affiliation><inist:fA14 i1="11"><s1>Department of Conservation and Research, Parque Zoologico Buin Zoo</s1><s2>Buin</s2><s3>CHL</s3><sZ>18 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Rottier, Peter" sort="Rottier, Peter" uniqKey="Rottier P" first="Peter" last="Rottier">Peter Rottier</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Osterhaus, Albert" sort="Osterhaus, Albert" uniqKey="Osterhaus A" first="Albert" last="Osterhaus">Albert Osterhaus</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="13"><s1>Viroclinics Biosciences BV</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>12 aut.</sZ><sZ>21 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gortazar Schmidt, Christian" sort="Gortazar Schmidt, Christian" uniqKey="Gortazar Schmidt C" first="Christian" last="Gortazar-Schmidt">Christian Gortazar-Schmidt</name><affiliation><inist:fA14 i1="12"><s1>SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real</s1><s3>ESP</s3><sZ>22 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0329482</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0329482 INIST</idno><idno type="RBID">Pascal:13-0329482</idno><idno type="wicri:Area/PascalFrancis/Corpus">000038</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study</title><author><name sortKey="Reusken, Chantal B E M" sort="Reusken, Chantal B E M" uniqKey="Reusken C" first="Chantal B. E. M." last="Reusken">Chantal B. E. M. Reusken</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L." last="Haagmans">Bart L. Haagmans</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A." last="Müller">Marcel A. Müller</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gutierrez, Carlos" sort="Gutierrez, Carlos" uniqKey="Gutierrez C" first="Carlos" last="Gutierrez">Carlos Gutierrez</name><affiliation><inist:fA14 i1="04"><s1>Department of Animal Medicine and Surgery, Veterinary Faculty, University of Las Palmas de Gran Canaria</s1><s2>Las Palmas, Canary Islands</s2><s3>ESP</s3><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Godeke, Gert Jan" sort="Godeke, Gert Jan" uniqKey="Godeke G" first="Gert-Jan" last="Godeke">Gert-Jan Godeke</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Meyer, Benjamin" sort="Meyer, Benjamin" uniqKey="Meyer B" first="Benjamin" last="Meyer">Benjamin Meyer</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Stalin Raj, V" sort="Stalin Raj, V" uniqKey="Stalin Raj V" first="V." last="Stalin Raj">V. Stalin Raj</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Smits De Vries, Laura" sort="Smits De Vries, Laura" uniqKey="Smits De Vries L" first="Laura" last="Smits-De Vries">Laura Smits-De Vries</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan-Felix" last="Drexler">Jan-Felix Drexler</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Smits, Saskia L" sort="Smits, Saskia L" uniqKey="Smits S" first="Saskia L." last="Smits">Saskia L. Smits</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="13"><s1>Viroclinics Biosciences BV</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>12 aut.</sZ><sZ>21 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="El Tahir, Yasmin E" sort="El Tahir, Yasmin E" uniqKey="El Tahir Y" first="Yasmin E." last="El Tahir">Yasmin E. El Tahir</name><affiliation><inist:fA14 i1="06"><s1>Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="De Sousa, Rita" sort="De Sousa, Rita" uniqKey="De Sousa R" first="Rita" last="De Sousa">Rita De Sousa</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="08"><s1>The European Programme for Public Health Microbiology Training, European Center for Disease Control</s1><s2>Stockholm</s2><s3>SWE</s3><sZ>14 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Van Beek, Janko" sort="Van Beek, Janko" uniqKey="Van Beek J" first="Janko" last="Van Beek">Janko Van Beek</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name><affiliation><inist:fA14 i1="07"><s1>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>16 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="09"><s1>Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>16 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Van Maanen, Kees" sort="Van Maanen, Kees" uniqKey="Van Maanen K" first="Kees" last="Van Maanen">Kees Van Maanen</name><affiliation><inist:fA14 i1="10"><s1>Animal Health Service</s1><s2>Deventer</s2><s3>NLD</s3><sZ>17 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Hidalgo Hermoso, Ezequiel" sort="Hidalgo Hermoso, Ezequiel" uniqKey="Hidalgo Hermoso E" first="Ezequiel" last="Hidalgo-Hermoso">Ezequiel Hidalgo-Hermoso</name><affiliation><inist:fA14 i1="11"><s1>Department of Conservation and Research, Parque Zoologico Buin Zoo</s1><s2>Buin</s2><s3>CHL</s3><sZ>18 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Rottier, Peter" sort="Rottier, Peter" uniqKey="Rottier P" first="Peter" last="Rottier">Peter Rottier</name><affiliation><inist:fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Osterhaus, Albert" sort="Osterhaus, Albert" uniqKey="Osterhaus A" first="Albert" last="Osterhaus">Albert Osterhaus</name><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="13"><s1>Viroclinics Biosciences BV</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>12 aut.</sZ><sZ>21 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gortazar Schmidt, Christian" sort="Gortazar Schmidt, Christian" uniqKey="Gortazar Schmidt C" first="Christian" last="Gortazar-Schmidt">Christian Gortazar-Schmidt</name><affiliation><inist:fA14 i1="12"><s1>SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real</s1><s3>ESP</s3><sZ>22 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation><inist:fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><affiliation><inist:fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></inist:fA14></affiliation></author></analytic><series><title level="j" type="main">Lancet. Infectious diseases : (print)</title><title level="j" type="abbreviated">Lancet. Infect. dis. : (print)</title><idno type="ISSN">1473-3099</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Lancet. Infectious diseases : (print)</title><title level="j" type="abbreviated">Lancet. Infect. dis. : (print)</title><idno type="ISSN">1473-3099</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Comparative study</term><term>Coronavirus</term><term>Middle East respiratory syndrome</term><term>Serum</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Sérum</term><term>Anticorps</term><term>Etude comparative</term><term>Coronavirus</term><term>Syndrome respiratoire du Moyen-Orient</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1473-3099</s0></fA01><fA03 i2="1"><s0>Lancet. Infect. dis. : (print)</s0></fA03><fA05><s2>13</s2></fA05><fA06><s2>10</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study</s1></fA08><fA11 i1="01" i2="1"><s1>REUSKEN (Chantal B. E. M.)</s1></fA11><fA11 i1="02" i2="1"><s1>HAAGMANS (Bart L.)</s1></fA11><fA11 i1="03" i2="1"><s1>MÜLLER (Marcel A.)</s1></fA11><fA11 i1="04" i2="1"><s1>GUTIERREZ (Carlos)</s1></fA11><fA11 i1="05" i2="1"><s1>GODEKE (Gert-Jan)</s1></fA11><fA11 i1="06" i2="1"><s1>MEYER (Benjamin)</s1></fA11><fA11 i1="07" i2="1"><s1>MUTH (Doreen)</s1></fA11><fA11 i1="08" i2="1"><s1>STALIN RAJ (V.)</s1></fA11><fA11 i1="09" i2="1"><s1>SMITS-DE VRIES (Laura)</s1></fA11><fA11 i1="10" i2="1"><s1>CORMAN (Victor M.)</s1></fA11><fA11 i1="11" i2="1"><s1>DREXLER (Jan-Felix)</s1></fA11><fA11 i1="12" i2="1"><s1>SMITS (Saskia L.)</s1></fA11><fA11 i1="13" i2="1"><s1>EL TAHIR (Yasmin E.)</s1></fA11><fA11 i1="14" i2="1"><s1>DE SOUSA (Rita)</s1></fA11><fA11 i1="15" i2="1"><s1>VAN BEEK (Janko)</s1></fA11><fA11 i1="16" i2="1"><s1>NOWOTNY (Norbert)</s1></fA11><fA11 i1="17" i2="1"><s1>VAN MAANEN (Kees)</s1></fA11><fA11 i1="18" i2="1"><s1>HIDALGO-HERMOSO (Ezequiel)</s1></fA11><fA11 i1="19" i2="1"><s1>BOSCH (Berend-Jan)</s1></fA11><fA11 i1="20" i2="1"><s1>ROTTIER (Peter)</s1></fA11><fA11 i1="21" i2="1"><s1>OSTERHAUS (Albert)</s1></fA11><fA11 i1="22" i2="1"><s1>GORTAZAR-SCHMIDT (Christian)</s1></fA11><fA11 i1="23" i2="1"><s1>DROSTEN (Christian)</s1></fA11><fA11 i1="24" i2="1"><s1>KOOPMANS (Marion P. G.)</s1></fA11><fA14 i1="01"><s1>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment</s1><s2>Bilthoven</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>24 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Viroscience, Erasmus Medical Centre</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>2 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>21 aut.</sZ><sZ>24 aut.</sZ></fA14><fA14 i1="03"><s1>Institute of Virology, University of Bonn Medical Centre</s1><s2>Bonn</s2><s3>DEU</s3><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>23 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Animal Medicine and Surgery, Veterinary Faculty, University of Las Palmas de Gran Canaria</s1><s2>Las Palmas, Canary Islands</s2><s3>ESP</s3><sZ>4 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine</s1><s2>Utrecht</s2><s3>NLD</s3><sZ>9 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>13 aut.</sZ></fA14><fA14 i1="07"><s1>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University</s1><s2>Muscat</s2><s3>OMN</s3><sZ>16 aut.</sZ></fA14><fA14 i1="08"><s1>The European Programme for Public Health Microbiology Training, European Center for Disease Control</s1><s2>Stockholm</s2><s3>SWE</s3><sZ>14 aut.</sZ></fA14><fA14 i1="09"><s1>Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna</s1><s2>Vienna</s2><s3>AUT</s3><sZ>16 aut.</sZ></fA14><fA14 i1="10"><s1>Animal Health Service</s1><s2>Deventer</s2><s3>NLD</s3><sZ>17 aut.</sZ></fA14><fA14 i1="11"><s1>Department of Conservation and Research, Parque Zoologico Buin Zoo</s1><s2>Buin</s2><s3>CHL</s3><sZ>18 aut.</sZ></fA14><fA14 i1="12"><s1>SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real</s1><s3>ESP</s3><sZ>22 aut.</sZ></fA14><fA14 i1="13"><s1>Viroclinics Biosciences BV</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>12 aut.</sZ><sZ>21 aut.</sZ></fA14><fA20><s1>859-866</s1></fA20><fA21><s1>2013</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>27478</s2><s5>354000505197280120</s5></fA43><fA44><s0>0000</s0><s1>© 2013 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>38 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>13-0329482</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Lancet. Infectious diseases : (print)</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Background A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.</s0></fC01><fC02 i1="01" i2="X"><s0>002B05C02C</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Sérum</s0><s5>07</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Serum</s0><s5>07</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Suero</s0><s5>07</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Anticorps</s0><s5>08</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Antibody</s0><s5>08</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>08</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Etude comparative</s0><s5>09</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Comparative study</s0><s5>09</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Estudio comparativo</s0><s5>09</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Syndrome respiratoire du Moyen-Orient</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Middle East respiratory syndrome</s0><s4>CD</s4><s5>96</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0><s5>37</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>37</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>37</s5></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0><s5>38</s5></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0><s5>38</s5></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0><s5>38</s5></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0></fC07><fN21><s1>308</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard><server><NO>PASCAL 13-0329482 INIST</NO><ET>Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study</ET><AU>REUSKEN (Chantal B. E. M.); HAAGMANS (Bart L.); MÜLLER (Marcel A.); GUTIERREZ (Carlos); GODEKE (Gert-Jan); MEYER (Benjamin); MUTH (Doreen); STALIN RAJ (V.); SMITS-DE VRIES (Laura); CORMAN (Victor M.); DREXLER (Jan-Felix); SMITS (Saskia L.); EL TAHIR (Yasmin E.); DE SOUSA (Rita); VAN BEEK (Janko); NOWOTNY (Norbert); VAN MAANEN (Kees); HIDALGO-HERMOSO (Ezequiel); BOSCH (Berend-Jan); ROTTIER (Peter); OSTERHAUS (Albert); GORTAZAR-SCHMIDT (Christian); DROSTEN (Christian); KOOPMANS (Marion P. G.)</AU><AF>Centre for Infectious Disease Research, Diagnostics and Screening, Division Virology, National Institute for Public Health and the Environment/Bilthoven/Pays-Bas (1 aut., 5 aut., 14 aut., 15 aut., 24 aut.); Department of Viroscience, Erasmus Medical Centre/Rotterdam/Pays-Bas (2 aut., 8 aut., 12 aut., 21 aut., 24 aut.); Institute of Virology, University of Bonn Medical Centre/Bonn/Allemagne (3 aut., 6 aut., 7 aut., 10 aut., 11 aut., 23 aut.); Department of Animal Medicine and Surgery, Veterinary Faculty, University of Las Palmas de Gran Canaria/Las Palmas, Canary Islands/Espagne (4 aut.); Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine/Utrecht/Pays-Bas (9 aut., 19 aut., 20 aut.); Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University/Muscat/Oman (13 aut.); Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University/Muscat/Oman (16 aut.); The European Programme for Public Health Microbiology Training, European Center for Disease Control/Stockholm/Suède (14 aut.); Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna/Vienna/Autriche (16 aut.); Animal Health Service/Deventer/Pays-Bas (17 aut.); Department of Conservation and Research, Parque Zoologico Buin Zoo/Buin/Chili (18 aut.); SaBio-IREC (CSIC-UCLM-JCCM), Ciudad Real/Espagne (22 aut.); Viroclinics Biosciences BV/Rotterdam/Pays-Bas (12 aut., 21 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Lancet. Infectious diseases : (print); ISSN 1473-3099; Royaume-Uni; Da. 2013; Vol. 13; No. 10; Pp. 859-866; Bibl. 38 ref.</SO><LA>Anglais</LA><EA>Background A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.</EA><CC>002B05C02C</CC><FD>Sérum; Anticorps; Etude comparative; Coronavirus; Syndrome respiratoire du Moyen-Orient</FD><FG>Coronaviridae; Nidovirales; Virus; Pathologie de l'appareil respiratoire; Virose; Infection</FG><ED>Serum; Antibody; Comparative study; Coronavirus; Middle East respiratory syndrome</ED><EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection</EG><SD>Suero; Anticuerpo; Estudio comparativo; Coronavirus</SD><LO>INIST-27478.354000505197280120</LO><ID>13-0329482</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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