MersV1, PascalFrancis, Checkpoint, bibRecord, 000062

Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit

Identifieur interne : 000062 ( PascalFrancis/Checkpoint ); précédent : 000061; suivant : 000063

Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit

Auteurs : David J. Vance [États-Unis] ; Nicholas J. Mantis [États-Unis]

Source :

Toxicon : (Oxford) [ 0041-0101 ] ; 2012.

RBID : Pascal:12-0344074

Descripteurs français

Pascal (Inist)
- Ricine, Neutralisation, Lymphocyte B, Déterminant antigénique, Solvant organique, Helix, Toxine, Sousunité, Bactériophage.

English descriptors

KwdEn :
- Antigenic determinant, B-Lymphocyte, Helix, Neutralization, Organic solvent, Phage, Ricin, Subunit, Toxin.

Abstract

Residues Y₉₁-T₁₁₆ of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q₉₈, E₁₀₂, T₁₀₅, and H₁₀₆ are central to mAb recognition.

Affiliations:

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Pascal:12-0344074

Le document en format XML

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<author><name sortKey="Vance, David J" sort="Vance, David J" uniqKey="Vance D" first="David J." last="Vance">David J. Vance</name>
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<author><name sortKey="Mantis, Nicholas J" sort="Mantis, Nicholas J" uniqKey="Mantis N" first="Nicholas J." last="Mantis">Nicholas J. Mantis</name>
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<s2>Albany, NY 12208</s2>
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<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biomedical Sciences, University at Albany School of Public Health</s1>
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<front><div type="abstract" xml:lang="en">Residues Y<sub>91</sub>
-T<sub>116</sub>
 of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q<sub>98</sub>
, E<sub>102</sub>
, T<sub>105</sub>
, and H<sub>106</sub>
 are central to mAb recognition.</div>
</front>
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<fC01 i1="01" l="ENG"><s0>Residues Y<sub>91</sub>
-T<sub>116</sub>
 of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q<sub>98</sub>
, E<sub>102</sub>
, T<sub>105</sub>
, and H<sub>106</sub>
 are central to mAb recognition.</s0>
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<fC02 i1="01" i2="X"><s0>002B03I</s0>
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<s5>05</s5>
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<s5>06</s5>
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<s5>06</s5>
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<fC03 i1="05" i2="X" l="SPA"><s0>Solvente orgánico</s0>
<s5>06</s5>
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<s2>NS</s2>
<s5>08</s5>
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<s5>09</s5>
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<s5>11</s5>
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<s5>11</s5>
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<s5>12</s5>
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<s2>NW</s2>
<s5>12</s5>
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<s2>NS</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Mollusca</s0>
<s2>NS</s2>
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<fN44 i1="01"><s1>OTO</s1>
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<name sortKey="Mantis, Nicholas J" sort="Mantis, Nicholas J" uniqKey="Mantis N" first="Nicholas J." last="Mantis">Nicholas J. Mantis</name>
<name sortKey="Mantis, Nicholas J" sort="Mantis, Nicholas J" uniqKey="Mantis N" first="Nicholas J." last="Mantis">Nicholas J. Mantis</name>
</country>
</tree>
</affiliations>
</record>

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Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit

Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri