Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors
Identifieur interne : 002C21 ( Ncbi/Merge ); précédent : 002C20; suivant : 002C22Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors
Auteurs : Libuse Trnkova [République tchèque] ; Frantisek Jelen [République tchèque] ; Jitka Petrlova [République tchèque] ; Vojtech Adam [République tchèque] ; David Potesil [République tchèque] ; Rene Kizek [République tchèque]Source :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2005.
Abstract
The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C) and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.
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PubMed Central: 3933885
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sort="Adam, Vojtech" uniqKey="Adam V" first="Vojtech" last="Adam">Vojtech Adam</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno</wicri:regionArea><wicri:noRegion>613 00 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Potesil, David" sort="Potesil, David" uniqKey="Potesil D" first="David" last="Potesil">David Potesil</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and 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Kotlarska 2, 611 37 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno</wicri:regionArea><wicri:noRegion>611 37 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Jelen, Frantisek" sort="Jelen, Frantisek" uniqKey="Jelen F" first="Frantisek" last="Jelen">Frantisek Jelen</name><affiliation wicri:level="1"><nlm:aff id="af2-sensors-05-00448"> Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno</wicri:regionArea><wicri:noRegion>612 65 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Petrlova, Jitka" sort="Petrlova, Jitka" uniqKey="Petrlova J" first="Jitka" last="Petrlova">Jitka Petrlova</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno</wicri:regionArea><wicri:noRegion>613 00 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Adam, Vojtech" sort="Adam, Vojtech" uniqKey="Adam V" first="Vojtech" last="Adam">Vojtech Adam</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno</wicri:regionArea><wicri:noRegion>613 00 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Potesil, David" sort="Potesil, David" uniqKey="Potesil D" first="David" last="Potesil">David Potesil</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno</wicri:regionArea><wicri:noRegion>613 00 Brno</wicri:noRegion></affiliation></author><author><name sortKey="Kizek, Rene" sort="Kizek, Rene" uniqKey="Kizek R" first="Rene" last="Kizek">Rene Kizek</name><affiliation wicri:level="1"><nlm:aff id="af3-sensors-05-00448"> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno</wicri:regionArea><wicri:noRegion>613 00 Brno</wicri:noRegion></affiliation></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="eISSN">1424-8220</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C) and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Watson, J D" uniqKey="Watson J">J.D. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sensors (Basel)</journal-id><journal-id journal-id-type="iso-abbrev">Sensors (Basel)</journal-id><journal-title-group><journal-title>Sensors (Basel, Switzerland)</journal-title></journal-title-group><issn pub-type="epub">1424-8220</issn><publisher><publisher-name>Molecular Diversity Preservation International (MDPI)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">3933885</article-id><article-id pub-id-type="publisher-id">sensors-05-00448</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Trnkova</surname><given-names>Libuse</given-names></name><xref ref-type="aff" rid="af1-sensors-05-00448"><sup>1</sup></xref><xref rid="c1-sensors-05-00448" ref-type="corresp"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jelen</surname><given-names>Frantisek</given-names></name><xref ref-type="aff" rid="af2-sensors-05-00448"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Petrlova</surname><given-names>Jitka</given-names></name><xref ref-type="aff" rid="af3-sensors-05-00448"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Adam</surname><given-names>Vojtech</given-names></name><xref ref-type="aff" rid="af3-sensors-05-00448"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Potesil</surname><given-names>David</given-names></name><xref ref-type="aff" rid="af3-sensors-05-00448"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kizek</surname><given-names>Rene</given-names></name><xref ref-type="aff" rid="af3-sensors-05-00448"><sup>3</sup></xref></contrib></contrib-group><aff id="af1-sensors-05-00448"><label>1</label> Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic</aff><aff id="af2-sensors-05-00448"><label>2</label> Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic</aff><aff id="af3-sensors-05-00448"><label>3</label> Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic</aff><author-notes><fn id="fn1-sensors-05-00448"><p>Dedicated to Professor Emil Palecek, on the Occasion of His 75<sup>th</sup> Birthday</p></fn><corresp id="c1-sensors-05-00448"><label>*</label> Author to whom correspondence should be addressed e-mail: <email>libuse@chemi.muni.cz</email></corresp></author-notes><pub-date pub-type="collection"><month>6</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>14</day><month>11</month><year>2005</year></pub-date><volume>5</volume><issue>6</issue><fpage>448</fpage><lpage>464</lpage><history><date date-type="received"><day>23</day><month>2</month><year>2005</year></date><date date-type="accepted"><day>04</day><month>4</month><year>2005</year></date></history><permissions><copyright-statement>© 2005 by MDPI (<ext-link ext-link-type="uri" xlink:href="http://www.mdpi.org">http://www.mdpi.org</ext-link>).</copyright-statement><copyright-year>2005</copyright-year><license><license-p>Reproduction is permitted for noncommercial purposes.</license-p></license></permissions><abstract><p>The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C) and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.</p></abstract><kwd-group><kwd>Oligodeoxynucleotide</kwd><kwd>DNA biosensor</kwd><kwd>linear sweep voltammetry</kwd><kwd>elimination voltammetry with linear scan</kwd><kwd>reduction of adenine and cytosine</kwd><kwd>mercury electrode</kwd><kwd>sequence of nucleotides</kwd></kwd-group></article-meta></front><floats-group><fig id="f1-sensors-05-00448" position="float"><label>Figure 1.</label><caption><p>Application of AdTS EVLS as a new detection method in DNA sensors. <bold>A)</bold> Adsorptive transfer stripping voltammetry – AdTSV: (<bold>1</bold>) hanging mercury drop electrode - HMDE; physico-chemical part of the biosensor, (<bold>2</bold>) modification of the working electrode surface by DNA – biological part of the biosensor; (<bold>3</bold>) washing of electrode with water; (<bold>4</bold>) electrochemical detection. <bold>B</bold>) Elimination voltammetry with linear scan – EVLS: linear sweep voltammetric data are treated by elimination procedure. <bold>C</bold>) Cytosine (C) and adenine (A); the functional groups, which are reduced, are marked by red frames.</p></caption><graphic xlink:href="sensors-05-00448f1"></graphic></fig><fig id="f2-sensors-05-00448" position="float"><label>Figure 2.</label><caption><p>Analysis of homo-oligodeoxynucleotides A9 and C9. <bold>A</bold>) Influence of pH (0.1 M phosphate buffer) on peak heights of ODNs. <bold>D</bold>) Elimination voltammograms of A9 and C9 suitable for the function eliminating kinetic and charging currents, and conserving the diffusion current (reference scan rate of 800 mV/s, pH 5.4). LSV experimental parameters: start potential 0 V, vertex potential −1.7 V, step potential 5mV, adsorption time 120s.</p></caption><graphic xlink:href="sensors-05-00448f2"></graphic></fig><fig id="f3-sensors-05-00448" position="float"><label>Figure 3.</label><caption><p>Analysis of hetero-oligodeoxynucleotides (9-mers): A3, A3a, A2 and A1. <bold>A</bold>) Voltammograms of ODNs (1μM) in the presence of 0.3 M ammonium formate and 0.05 M phosphate buffer (pH 6.9) at different scan rates (200, 400, 800, and 1 600 mV/s). <bold>B</bold>) Dependence of ODNs current responses on scan rate. <bold>C</bold>) Influence of pH (0.1 M phosphate buffer) on heights of ODNs signals. <bold>D</bold>) Elimination voltammograms of ODNs suitable for the function eliminating kinetic and charging currents, and conserving the diffusion current (reference scan rate of 800 mV/s, pH 5.4) For other details see <xref rid="f2-sensors-05-00448" ref-type="fig">Fig. 2</xref>.</p></caption><graphic xlink:href="sensors-05-00448f3"></graphic></fig><fig id="f4-sensors-05-00448" position="float"><label>Figure 4.</label><caption><p>Analysis of hetero-oligodeoxynucleotides (20-mers): ODN(20a), ODN(20b), and ODN(20c) with the A:C ratio the in primary structure of ODNs of 3:9, 5:1, and 7:1, respectively. <bold>A</bold>) Voltammograms of ODNs (1μM) in the presence of 0.3 M ammonium formate and 0.05 M phosphate buffer (pH 6.9) at different scan rates (200, 400, 800 and 1 600 mV/s). <bold>B</bold>) Dependence of ODNs current responses on scan rate. <bold>C</bold>) Influence of pH (0.1 M phosphate buffer) on the height of ODNs signals. <bold>D</bold>) Elimination voltammograms of ODNs suitable for the function eliminating kinetic and charging currents, and conserving the diffusion current (reference scan rate of 800 mV/s, pH 5.4). For other details see <xref rid="f2-sensors-05-00448" ref-type="fig">Fig. 2</xref>.</p></caption><graphic xlink:href="sensors-05-00448f4"></graphic></fig><table-wrap id="t1-sensors-05-00448" position="float"><label>Table 1.</label><caption><p>Characterization of ODNs (20-mers) by the ratio of A:C in ODNs (pH 5.4).</p></caption><table frame="hsides" rules="cols"><thead><tr><th align="center" valign="middle" rowspan="1" colspan="1">ODNs</th><th align="center" valign="middle" rowspan="1" colspan="1">Content of adenine</th><th align="center" valign="middle" rowspan="1" colspan="1">Content of cytosine</th><th align="center" valign="middle" rowspan="1" colspan="1">True Ratio value<xref rid="tfn2-sensors-05-00448" ref-type="table-fn"><sup>1</sup></xref></th><th align="center" valign="middle" rowspan="1" colspan="1">EVLS experimental Ratio value<xref rid="tfn3-sensors-05-00448" ref-type="table-fn"><sup>2</sup></xref></th><th align="center" valign="middle" rowspan="1" colspan="1">Difference between experimental and true Ratio value<xref rid="tfn4-sensors-05-00448" ref-type="table-fn"><sup>3</sup></xref></th><th align="center" valign="middle" rowspan="1" colspan="1">Difference between experimental and true Ratio value (%)<xref rid="tfn5-sensors-05-00448" ref-type="table-fn"><sup>4</sup></xref></th></tr><tr><th valign="bottom" colspan="7" rowspan="1"><hr></hr></th></tr></thead><tbody><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN(20a)</td><td align="center" valign="top" rowspan="1" colspan="1">3</td><td align="center" valign="top" rowspan="1" colspan="1">9</td><td align="center" valign="top" rowspan="1" colspan="1">0.33</td><td align="center" valign="top" rowspan="1" colspan="1">0.39</td><td align="center" valign="top" rowspan="1" colspan="1">0.06</td><td align="center" valign="top" rowspan="1" colspan="1">18</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN(20b)</td><td align="center" valign="top" rowspan="1" colspan="1">5</td><td align="center" valign="top" rowspan="1" colspan="1">1</td><td align="center" valign="top" rowspan="1" colspan="1">5</td><td align="center" valign="top" rowspan="1" colspan="1">4.65</td><td align="center" valign="top" rowspan="1" colspan="1">-0.35</td><td align="center" valign="top" rowspan="1" colspan="1">7</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN(20c)</td><td align="center" valign="top" rowspan="1" colspan="1">7</td><td align="center" valign="top" rowspan="1" colspan="1">1</td><td align="center" valign="top" rowspan="1" colspan="1">7</td><td align="center" valign="top" rowspan="1" colspan="1">6.34</td><td align="center" valign="top" rowspan="1" colspan="1">-0.66</td><td align="center" valign="top" rowspan="1" colspan="1">1</td></tr></tbody></table><table-wrap-foot><fn id="tfn2-sensors-05-00448"><label>1.</label><p>… True ratio value = Content of adenine/Content of cytosine</p></fn><fn id="tfn3-sensors-05-00448"><label>2.</label><p>… EVLS experimental Ratio value = EVLS adenine peak height/EVLS cytosine peak height</p></fn><fn id="tfn4-sensors-05-00448"><label>3.</label><p>… Difference between true and experimental Ratio value = EVLS experimental Ratio value - True ratio value</p></fn><fn id="tfn5-sensors-05-00448"><label>4.</label><p>… Difference between experimental and True Ratio value (%) = (|Difference between true and experimental Ratio value|*100;/True ratio value</p></fn></table-wrap-foot></table-wrap><table-wrap id="t2-sensors-05-00448" position="float"><label>Table 2.</label><caption><p>EVLS detection limits of ODNs.</p></caption><table frame="hsides" rules="cols"><thead><tr><th align="center" valign="middle" rowspan="1" colspan="1"><underline>ODNs</underline></th><th align="center" valign="middle" rowspan="1" colspan="1">LD<xref rid="tfn1-sensors-05-00448" ref-type="table-fn"><sup>a</sup></xref> (nM)</th></tr><tr><th valign="bottom" colspan="2" rowspan="1"><hr></hr></th></tr></thead><tbody><tr><td align="center" valign="top" rowspan="1" colspan="1">A9</td><td align="center" valign="top" rowspan="1" colspan="1">0.55</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">C9</td><td align="center" valign="top" rowspan="1" colspan="1">1.6</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">A3</td><td align="center" valign="top" rowspan="1" colspan="1">1.2</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">A3a</td><td align="center" valign="top" rowspan="1" colspan="1">0.24</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">A2</td><td align="center" valign="top" rowspan="1" colspan="1">0.19</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">A1</td><td align="center" valign="top" rowspan="1" colspan="1">0.18</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN (20a)</td><td align="center" valign="top" rowspan="1" colspan="1">0.47</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN (20b)</td><td align="center" valign="top" rowspan="1" colspan="1">0.47</td></tr><tr><td align="center" valign="top" rowspan="1" colspan="1">ODN (20c)</td><td align="center" valign="top" rowspan="1" colspan="1">0.56</td></tr></tbody></table><table-wrap-foot><fn id="tfn1-sensors-05-00448"><label>a</label><p>… Limit of detection LD was expressed as 3 S/N ratio.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list><country><li>République tchèque</li></country></list><tree><country name="République tchèque"><noRegion><name sortKey="Trnkova, Libuse" sort="Trnkova, Libuse" uniqKey="Trnkova L" first="Libuse" last="Trnkova">Libuse Trnkova</name></noRegion><name sortKey="Adam, Vojtech" sort="Adam, Vojtech" uniqKey="Adam V" first="Vojtech" last="Adam">Vojtech Adam</name><name sortKey="Jelen, Frantisek" sort="Jelen, Frantisek" uniqKey="Jelen F" first="Frantisek" last="Jelen">Frantisek Jelen</name><name sortKey="Kizek, Rene" sort="Kizek, Rene" uniqKey="Kizek R" first="Rene" last="Kizek">Rene Kizek</name><name sortKey="Petrlova, Jitka" sort="Petrlova, Jitka" uniqKey="Petrlova J" first="Jitka" last="Petrlova">Jitka Petrlova</name><name sortKey="Potesil, David" sort="Potesil, David" uniqKey="Potesil D" first="David" last="Potesil">David Potesil</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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