Efficient replication and shedding of MERS CoV from the upper respiratory tract of experimentally infected dromedary camels
Identifieur interne : 002C19 ( Ncbi/Merge ); précédent : 002C18; suivant : 002C20Efficient replication and shedding of MERS CoV from the upper respiratory tract of experimentally infected dromedary camels
Auteurs : Dr Adney ; Vr Brown ; N. Van Doremalen ; T. Bushmaker ; D. Scott ; E. De Wit ; Va Munster ; Ra BowenSource :
- New Horizons in Translational Medicine [ 2352-7536 ] ; 2015.
Abstract
The Middle East respiratory syndrome coronavirus (MERS CoV) is a novel coronavirus first recognized in 2012 and is associated with severe respiratory disease in humans. Virus has been isolated from dromedary camels in endemic areas, and many camels also have neutralizing antibodies against the virus, suggesting that they are likely a reservoir host. In order to better understand the role of camels in virus transmission we experimentally infected 3 adult, male dromedary camels with a human isolate of MERS CoV. All animals developed a transient, upper respiratory tract infection associated with very minor clinical disease. Large quantities of infectious virus were isolated from nasal secretions from each animal through 7 days post-inoculation, and viral RNA was detected much longer. Although our study design was limited to 3 animals, these data indicate that MERS CoV readily infects camels, which shed large amounts of virus and likely can efficiently transmit virus to other camels and humans.
Url:
DOI: 10.1016/j.nhtm.2015.07.056
PubMed: NONE
PubMed Central: 7129280
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<front><div type="abstract" xml:lang="en"><p>The Middle East respiratory syndrome coronavirus (MERS CoV) is a novel coronavirus first recognized in 2012 and is associated with severe respiratory disease in humans. Virus has been isolated from dromedary camels in endemic areas, and many camels also have neutralizing antibodies against the virus, suggesting that they are likely a reservoir host. In order to better understand the role of camels in virus transmission we experimentally infected 3 adult, male dromedary camels with a human isolate of MERS CoV. All animals developed a transient, upper respiratory tract infection associated with very minor clinical disease. Large quantities of infectious virus were isolated from nasal secretions from each animal through 7 days post-inoculation, and viral RNA was detected much longer. Although our study design was limited to 3 animals, these data indicate that MERS CoV readily infects camels, which shed large amounts of virus and likely can efficiently transmit virus to other camels and humans.</p>
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Department of Microbiology, Immunology, and Pathology, Colorado State University</aff>
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Laboratory of Virology, Rocky Mountain Laboratories, NIAID</aff>
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Rocky Mountain Veterinary Branch, Division of Intramural Research NIAID</aff>
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<abstract id="ab0090"><p>The Middle East respiratory syndrome coronavirus (MERS CoV) is a novel coronavirus first recognized in 2012 and is associated with severe respiratory disease in humans. Virus has been isolated from dromedary camels in endemic areas, and many camels also have neutralizing antibodies against the virus, suggesting that they are likely a reservoir host. In order to better understand the role of camels in virus transmission we experimentally infected 3 adult, male dromedary camels with a human isolate of MERS CoV. All animals developed a transient, upper respiratory tract infection associated with very minor clinical disease. Large quantities of infectious virus were isolated from nasal secretions from each animal through 7 days post-inoculation, and viral RNA was detected much longer. Although our study design was limited to 3 animals, these data indicate that MERS CoV readily infects camels, which shed large amounts of virus and likely can efficiently transmit virus to other camels and humans.</p>
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<name sortKey="Brown, Vr" sort="Brown, Vr" uniqKey="Brown V" first="Vr" last="Brown">Vr Brown</name>
<name sortKey="Bushmaker, T" sort="Bushmaker, T" uniqKey="Bushmaker T" first="T" last="Bushmaker">T. Bushmaker</name>
<name sortKey="De Wit, E" sort="De Wit, E" uniqKey="De Wit E" first="E" last="De Wit">E. De Wit</name>
<name sortKey="Munster, Va" sort="Munster, Va" uniqKey="Munster V" first="Va" last="Munster">Va Munster</name>
<name sortKey="Scott, D" sort="Scott, D" uniqKey="Scott D" first="D" last="Scott">D. Scott</name>
<name sortKey="Van Doremalen, N" sort="Van Doremalen, N" uniqKey="Van Doremalen N" first="N" last="Van Doremalen">N. Van Doremalen</name>
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