Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.
Identifieur interne : 002A21 ( Ncbi/Merge ); précédent : 002A20; suivant : 002A22Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.
Auteurs : L D Graham [Australie] ; S M Mitchell ; P A UnderwoodSource :
- Biochemistry and molecular biology international [ 1039-9712 ] ; 1995.
Descripteurs français
- KwdFr :
- Antienzymes (pharmacologie), Antienzymes (synthèse chimique), Données de séquences moléculaires, Humains, Oligonucléotides (pharmacologie), Oligonucléotides (synthèse chimique), Plaquettes (enzymologie), Polysaccharide-lyases (antagonistes et inhibiteurs), Polysaccharide-lyases (métabolisme), Séquence nucléotidique, Thionucléotides (pharmacologie), Thionucléotides (synthèse chimique).
- MESH :
- antagonistes et inhibiteurs : Polysaccharide-lyases.
- enzymologie : Plaquettes.
- métabolisme : Polysaccharide-lyases.
- pharmacologie : Antienzymes, Oligonucléotides, Thionucléotides.
- synthèse chimique : Antienzymes, Oligonucléotides, Thionucléotides.
- Données de séquences moléculaires, Humains, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Blood Platelets (enzymology), Enzyme Inhibitors (chemical synthesis), Enzyme Inhibitors (pharmacology), Humans, Molecular Sequence Data, Oligonucleotides (chemical synthesis), Oligonucleotides (pharmacology), Polysaccharide-Lyases (antagonists & inhibitors), Polysaccharide-Lyases (metabolism), Thionucleotides (chemical synthesis), Thionucleotides (pharmacology).
- MESH :
- chemical , antagonists & inhibitors : Polysaccharide-Lyases.
- chemical , chemical synthesis : Enzyme Inhibitors, Oligonucleotides, Thionucleotides.
- enzymology : Blood Platelets.
- chemical , metabolism : Polysaccharide-Lyases.
- chemical , pharmacology : Enzyme Inhibitors, Oligonucleotides, Thionucleotides.
- Base Sequence, Humans, Molecular Sequence Data.
Abstract
In view of the role that heparitinase and heparanase enzymes are thought to play in regulating the proliferation of smooth muscle cells, inhibitors of these enzymes may have therapeutic value in the treatment of vascular hyperplasia. Here we report that phosphorothioate oligodeoxynucleotides inhibit platelet heparitinase and related enzymes in vitro. The inhibition is greatly enhanced by the presence of a GGG motif in the oligonucleotide, and also increases with oligonucleotide for two phosphorothioate DNA 30-mers consisting solely of guanosine and thymidine nucleotides. Their inhibitory efficacy was greater when heparan sulphate was used as substrate.
PubMed: 8673006
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002801
- to stream PubMed, to step Curation: 002801
- to stream PubMed, to step Checkpoint: 002672
Links to Exploration step
pubmed:8673006Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.</title><author><name sortKey="Graham, L D" sort="Graham, L D" uniqKey="Graham L" first="L D" last="Graham">L D Graham</name><affiliation wicri:level="1"><nlm:affiliation>CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, S M" sort="Mitchell, S M" uniqKey="Mitchell S" first="S M" last="Mitchell">S M Mitchell</name></author><author><name sortKey="Underwood, P A" sort="Underwood, P A" uniqKey="Underwood P" first="P A" last="Underwood">P A Underwood</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:8673006</idno><idno type="pmid">8673006</idno><idno type="wicri:Area/PubMed/Corpus">002801</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002801</idno><idno type="wicri:Area/PubMed/Curation">002801</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002801</idno><idno type="wicri:Area/PubMed/Checkpoint">002672</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002672</idno><idno type="wicri:Area/Ncbi/Merge">002A21</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.</title><author><name sortKey="Graham, L D" sort="Graham, L D" uniqKey="Graham L" first="L D" last="Graham">L D Graham</name><affiliation wicri:level="1"><nlm:affiliation>CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, S M" sort="Mitchell, S M" uniqKey="Mitchell S" first="S M" last="Mitchell">S M Mitchell</name></author><author><name sortKey="Underwood, P A" sort="Underwood, P A" uniqKey="Underwood P" first="P A" last="Underwood">P A Underwood</name></author></analytic><series><title level="j">Biochemistry and molecular biology international</title><idno type="ISSN">1039-9712</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Blood Platelets (enzymology)</term><term>Enzyme Inhibitors (chemical synthesis)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Oligonucleotides (chemical synthesis)</term><term>Oligonucleotides (pharmacology)</term><term>Polysaccharide-Lyases (antagonists & inhibitors)</term><term>Polysaccharide-Lyases (metabolism)</term><term>Thionucleotides (chemical synthesis)</term><term>Thionucleotides (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antienzymes (pharmacologie)</term><term>Antienzymes (synthèse chimique)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Oligonucléotides (pharmacologie)</term><term>Oligonucléotides (synthèse chimique)</term><term>Plaquettes (enzymologie)</term><term>Polysaccharide-lyases (antagonistes et inhibiteurs)</term><term>Polysaccharide-lyases (métabolisme)</term><term>Séquence nucléotidique</term><term>Thionucléotides (pharmacologie)</term><term>Thionucléotides (synthèse chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Polysaccharide-Lyases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Enzyme Inhibitors</term><term>Oligonucleotides</term><term>Thionucleotides</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Polysaccharide-lyases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Plaquettes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Blood Platelets</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Polysaccharide-Lyases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Polysaccharide-lyases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Oligonucléotides</term><term>Thionucléotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term><term>Oligonucleotides</term><term>Thionucleotides</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Antienzymes</term><term>Oligonucléotides</term><term>Thionucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Humains</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In view of the role that heparitinase and heparanase enzymes are thought to play in regulating the proliferation of smooth muscle cells, inhibitors of these enzymes may have therapeutic value in the treatment of vascular hyperplasia. Here we report that phosphorothioate oligodeoxynucleotides inhibit platelet heparitinase and related enzymes in vitro. The inhibition is greatly enhanced by the presence of a GGG motif in the oligonucleotide, and also increases with oligonucleotide for two phosphorothioate DNA 30-mers consisting solely of guanosine and thymidine nucleotides. Their inhibitory efficacy was greater when heparan sulphate was used as substrate.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8673006</PMID><DateCompleted><Year>1996</Year><Month>08</Month><Day>13</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1039-9712</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>2</Issue><PubDate><Year>1995</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Biochemistry and molecular biology international</Title><ISOAbbreviation>Biochem. Mol. Biol. Int.</ISOAbbreviation></Journal><ArticleTitle>Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.</ArticleTitle><Pagination><MedlinePgn>239-46</MedlinePgn></Pagination><Abstract><AbstractText>In view of the role that heparitinase and heparanase enzymes are thought to play in regulating the proliferation of smooth muscle cells, inhibitors of these enzymes may have therapeutic value in the treatment of vascular hyperplasia. Here we report that phosphorothioate oligodeoxynucleotides inhibit platelet heparitinase and related enzymes in vitro. The inhibition is greatly enhanced by the presence of a GGG motif in the oligonucleotide, and also increases with oligonucleotide for two phosphorothioate DNA 30-mers consisting solely of guanosine and thymidine nucleotides. Their inhibitory efficacy was greater when heparan sulphate was used as substrate.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Graham</LastName><ForeName>L D</ForeName><Initials>LD</Initials><AffiliationInfo><Affiliation>CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mitchell</LastName><ForeName>S M</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Underwood</LastName><ForeName>P A</ForeName><Initials>PA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biochem Mol Biol Int</MedlineTA><NlmUniqueID>9306673</NlmUniqueID><ISSNLinking>1039-9712</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009841">Oligonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013873">Thionucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.2.2.-</RegistryNumber><NameOfSubstance UI="D011133">Polysaccharide-Lyases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.2.2.8</RegistryNumber><NameOfSubstance UI="C021349">heparitinsulfate lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001792" MajorTopicYN="N">Blood Platelets</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009841" MajorTopicYN="N">Oligonucleotides</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011133" MajorTopicYN="N">Polysaccharide-Lyases</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013873" MajorTopicYN="N">Thionucleotides</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8673006</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Mitchell, S M" sort="Mitchell, S M" uniqKey="Mitchell S" first="S M" last="Mitchell">S M Mitchell</name><name sortKey="Underwood, P A" sort="Underwood, P A" uniqKey="Underwood P" first="P A" last="Underwood">P A Underwood</name></noCountry><country name="Australie"><noRegion><name sortKey="Graham, L D" sort="Graham, L D" uniqKey="Graham L" first="L D" last="Graham">L D Graham</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A21 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 002A21 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:8673006
|texte= Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:8673006" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |