Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.
Identifieur interne : 002A21 ( Ncbi/Merge ); précédent : 002A20; suivant : 002A22Inhibition of platelet heparitinase by phosphorothioate DNA oligonucleotides.
Auteurs : L D Graham [Australie] ; S M Mitchell ; P A UnderwoodSource :
- Biochemistry and molecular biology international [ 1039-9712 ] ; 1995.
Descripteurs français
- KwdFr :
- Antienzymes (pharmacologie), Antienzymes (synthèse chimique), Données de séquences moléculaires, Humains, Oligonucléotides (pharmacologie), Oligonucléotides (synthèse chimique), Plaquettes (enzymologie), Polysaccharide-lyases (antagonistes et inhibiteurs), Polysaccharide-lyases (métabolisme), Séquence nucléotidique, Thionucléotides (pharmacologie), Thionucléotides (synthèse chimique).
- MESH :
- antagonistes et inhibiteurs : Polysaccharide-lyases.
- enzymologie : Plaquettes.
- métabolisme : Polysaccharide-lyases.
- pharmacologie : Antienzymes, Oligonucléotides, Thionucléotides.
- synthèse chimique : Antienzymes, Oligonucléotides, Thionucléotides.
- Données de séquences moléculaires, Humains, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Blood Platelets (enzymology), Enzyme Inhibitors (chemical synthesis), Enzyme Inhibitors (pharmacology), Humans, Molecular Sequence Data, Oligonucleotides (chemical synthesis), Oligonucleotides (pharmacology), Polysaccharide-Lyases (antagonists & inhibitors), Polysaccharide-Lyases (metabolism), Thionucleotides (chemical synthesis), Thionucleotides (pharmacology).
- MESH :
- chemical , antagonists & inhibitors : Polysaccharide-Lyases.
- chemical , chemical synthesis : Enzyme Inhibitors, Oligonucleotides, Thionucleotides.
- enzymology : Blood Platelets.
- chemical , metabolism : Polysaccharide-Lyases.
- chemical , pharmacology : Enzyme Inhibitors, Oligonucleotides, Thionucleotides.
- Base Sequence, Humans, Molecular Sequence Data.
Abstract
In view of the role that heparitinase and heparanase enzymes are thought to play in regulating the proliferation of smooth muscle cells, inhibitors of these enzymes may have therapeutic value in the treatment of vascular hyperplasia. Here we report that phosphorothioate oligodeoxynucleotides inhibit platelet heparitinase and related enzymes in vitro. The inhibition is greatly enhanced by the presence of a GGG motif in the oligonucleotide, and also increases with oligonucleotide for two phosphorothioate DNA 30-mers consisting solely of guanosine and thymidine nucleotides. Their inhibitory efficacy was greater when heparan sulphate was used as substrate.
PubMed: 8673006
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pubmed:8673006Le document en format XML
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">In view of the role that heparitinase and heparanase enzymes are thought to play in regulating the proliferation of smooth muscle cells, inhibitors of these enzymes may have therapeutic value in the treatment of vascular hyperplasia. Here we report that phosphorothioate oligodeoxynucleotides inhibit platelet heparitinase and related enzymes in vitro. The inhibition is greatly enhanced by the presence of a GGG motif in the oligonucleotide, and also increases with oligonucleotide for two phosphorothioate DNA 30-mers consisting solely of guanosine and thymidine nucleotides. Their inhibitory efficacy was greater when heparan sulphate was used as substrate.</div>
</front>
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