Measurement of bile salt aggregation equilibria using kinetic dialysis and spreadsheet modeling.
Identifieur interne : 002A05 ( Ncbi/Merge ); précédent : 002A04; suivant : 002A06Measurement of bile salt aggregation equilibria using kinetic dialysis and spreadsheet modeling.
Auteurs : W C Duane [États-Unis] ; D P GilboeSource :
- Analytical biochemistry [ 0003-2697 ] ; 1995.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Bile Acids and Salts, Taurocholic Acid.
- chemical , chemistry : Bile Acids and Salts, Taurocholic Acid.
- Dialysis, Kinetics, Light, Macromolecular Substances, Micelles, Models, Chemical, Scattering, Radiation, Software.
Abstract
Bile salts initially self-associate into small aggregates such as dimers and trimers before forming micelles at higher concentrations. The very size of these small aggregates hampers quantitation of their formation. We have devised a method using kinetic dialysis measurements to quantitatively examine aggregation of bile salt at concentrations below 10 mM. Data for rate of dialysis versus concentration were fitted to hypothetical models of aggregation using a personal computer spreadsheet. For sodium taurocholate these data best fit a model of initial dimer and trimer formation with stepwise association constants of 100 and 160, respectively. Addition of larger aggregates to the model did not improve the fit. For sodium taurodeoxycholate the data best fit a model which included not only dimers and trimers, but also tetramers or larger aggregates up to 10-mers with stepwise association constants of 40, 400, and 1700 for dimers, trimers, and tetramers, respectively. These data agree reasonably well with existing literature and suggest that kinetic dialysis with spreadsheet modeling is a useful technique for the study of bile salt aggregation at relatively low concentrations.
DOI: 10.1006/abio.1995.1372
PubMed: 8533889
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The very size of these small aggregates hampers quantitation of their formation. We have devised a method using kinetic dialysis measurements to quantitatively examine aggregation of bile salt at concentrations below 10 mM. Data for rate of dialysis versus concentration were fitted to hypothetical models of aggregation using a personal computer spreadsheet. For sodium taurocholate these data best fit a model of initial dimer and trimer formation with stepwise association constants of 100 and 160, respectively. Addition of larger aggregates to the model did not improve the fit. For sodium taurodeoxycholate the data best fit a model which included not only dimers and trimers, but also tetramers or larger aggregates up to 10-mers with stepwise association constants of 40, 400, and 1700 for dimers, trimers, and tetramers, respectively. These data agree reasonably well with existing literature and suggest that kinetic dialysis with spreadsheet modeling is a useful technique for the study of bile salt aggregation at relatively low concentrations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8533889</PMID><DateCompleted><Year>1996</Year><Month>02</Month><Day>01</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-2697</ISSN><JournalIssue CitedMedium="Print"><Volume>229</Volume><Issue>1</Issue><PubDate><Year>1995</Year><Month>Jul</Month><Day>20</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal. Biochem.</ISOAbbreviation></Journal><ArticleTitle>Measurement of bile salt aggregation equilibria using kinetic dialysis and spreadsheet modeling.</ArticleTitle><Pagination><MedlinePgn>15-9</MedlinePgn></Pagination><Abstract><AbstractText>Bile salts initially self-associate into small aggregates such as dimers and trimers before forming micelles at higher concentrations. The very size of these small aggregates hampers quantitation of their formation. We have devised a method using kinetic dialysis measurements to quantitatively examine aggregation of bile salt at concentrations below 10 mM. Data for rate of dialysis versus concentration were fitted to hypothetical models of aggregation using a personal computer spreadsheet. For sodium taurocholate these data best fit a model of initial dimer and trimer formation with stepwise association constants of 100 and 160, respectively. Addition of larger aggregates to the model did not improve the fit. For sodium taurodeoxycholate the data best fit a model which included not only dimers and trimers, but also tetramers or larger aggregates up to 10-mers with stepwise association constants of 40, 400, and 1700 for dimers, trimers, and tetramers, respectively. These data agree reasonably well with existing literature and suggest that kinetic dialysis with spreadsheet modeling is a useful technique for the study of bile salt aggregation at relatively low concentrations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Duane</LastName><ForeName>W C</ForeName><Initials>WC</Initials><AffiliationInfo><Affiliation>Department of Medicine, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gilboe</LastName><ForeName>D P</ForeName><Initials>DP</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01-DK42433</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001647">Bile Acids and Salts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D046911">Macromolecular Substances</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008823">Micelles</NameOfSubstance></Chemical><Chemical><RegistryNumber>5E090O0G3Z</RegistryNumber><NameOfSubstance UI="D013656">Taurocholic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001647" MajorTopicYN="N">Bile Acids and Salts</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003956" MajorTopicYN="N">Dialysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008027" MajorTopicYN="N">Light</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046911" MajorTopicYN="N">Macromolecular Substances</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008823" MajorTopicYN="N">Micelles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008956" MajorTopicYN="N">Models, Chemical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012542" MajorTopicYN="N">Scattering, Radiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012984" MajorTopicYN="N">Software</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013656" MajorTopicYN="N">Taurocholic Acid</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>7</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>7</Month><Day>20</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>7</Month><Day>20</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8533889</ArticleId><ArticleId IdType="pii">S0003-2697(85)71372-3</ArticleId><ArticleId IdType="doi">10.1006/abio.1995.1372</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Minnesota</li></region></list><tree><noCountry><name sortKey="Gilboe, D P" sort="Gilboe, D P" uniqKey="Gilboe D" first="D P" last="Gilboe">D P Gilboe</name></noCountry><country name="États-Unis"><region name="Minnesota"><name sortKey="Duane, W C" sort="Duane, W C" uniqKey="Duane W" first="W C" last="Duane">W C Duane</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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