A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.
Identifieur interne : 001236 ( Ncbi/Merge ); précédent : 001235; suivant : 001237A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.
Auteurs : Karuppiah Muthumani [États-Unis] ; Darryl Falzarano [États-Unis] ; Emma L. Reuschel [États-Unis] ; Colleen Tingey [États-Unis] ; Seleeke Flingai [États-Unis] ; Daniel O. Villarreal [États-Unis] ; Megan Wise [États-Unis] ; Ami Patel [États-Unis] ; Abdullah Izmirly [États-Unis] ; Abdulelah Aljuaid [États-Unis] ; Alecia M. Seliga [États-Unis] ; Geoff Soule [Canada] ; Matthew Morrow [États-Unis] ; Kimberly A. Kraynyak [États-Unis] ; Amir S. Khan [États-Unis] ; Dana P. Scott [États-Unis] ; Friederike Feldmann [États-Unis] ; Rachel Lacasse [États-Unis] ; Kimberly Meade-White [États-Unis] ; Atsushi Okumura [États-Unis] ; Kenneth E. Ugen [États-Unis] ; Niranjan Y. Sardesai [États-Unis] ; J Joseph Kim [États-Unis] ; Gary Kobinger [Canada] ; Heinz Feldmann [États-Unis] ; David B. Weiner [États-Unis]Source :
- Science translational medicine [ 1946-6242 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient.
- usage thérapeutique : Vaccins à ADN.
- Animaux, Chameaux, Macaca mulatta, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- chemical , therapeutic use : Vaccines, DNA.
- Animals, Camelus, Macaca mulatta, Mice.
Abstract
First identified in 2012, Middle East respiratory syndrome (MERS) is caused by an emerging human coronavirus, which is distinct from the severe acute respiratory syndrome coronavirus (SARS-CoV), and represents a novel member of the lineage C betacoronoviruses. Since its identification, MERS coronavirus (MERS-CoV) has been linked to more than 1372 infections manifesting with severe morbidity and, often, mortality (about 495 deaths) in the Arabian Peninsula, Europe, and, most recently, the United States. Human-to-human transmission has been documented, with nosocomial transmission appearing to be an important route of infection. The recent increase in cases of MERS in the Middle East coupled with the lack of approved antiviral therapies or vaccines to treat or prevent this infection are causes for concern. We report on the development of a synthetic DNA vaccine against MERS-CoV. An optimized DNA vaccine encoding the MERS spike protein induced potent cellular immunity and antigen-specific neutralizing antibodies in mice, macaques, and camels. Vaccinated rhesus macaques seroconverted rapidly and exhibited high levels of virus-neutralizing activity. Upon MERS viral challenge, all of the monkeys in the control-vaccinated group developed characteristic disease, including pneumonia. Vaccinated macaques were protected and failed to demonstrate any clinical or radiographic signs of pneumonia. These studies demonstrate that a consensus MERS spike protein synthetic DNA vaccine can induce protective responses against viral challenge, indicating that this strategy may have value as a possible vaccine modality against this emerging pathogen.
DOI: 10.1126/scitranslmed.aac7462
PubMed: 26290414
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001502
- to stream PubMed, to step Curation: 001502
- to stream PubMed, to step Checkpoint: 001654
Links to Exploration step
pubmed:26290414Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.</title><author><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Reuschel, Emma L" sort="Reuschel, Emma L" uniqKey="Reuschel E" first="Emma L" last="Reuschel">Emma L. Reuschel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Tingey, Colleen" sort="Tingey, Colleen" uniqKey="Tingey C" first="Colleen" last="Tingey">Colleen Tingey</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Flingai, Seleeke" sort="Flingai, Seleeke" uniqKey="Flingai S" first="Seleeke" last="Flingai">Seleeke Flingai</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Villarreal, Daniel O" sort="Villarreal, Daniel O" uniqKey="Villarreal D" first="Daniel O" last="Villarreal">Daniel O. Villarreal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Wise, Megan" sort="Wise, Megan" uniqKey="Wise M" first="Megan" last="Wise">Megan Wise</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Patel, Ami" sort="Patel, Ami" uniqKey="Patel A" first="Ami" last="Patel">Ami Patel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Izmirly, Abdullah" sort="Izmirly, Abdullah" uniqKey="Izmirly A" first="Abdullah" last="Izmirly">Abdullah Izmirly</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Aljuaid, Abdulelah" sort="Aljuaid, Abdulelah" uniqKey="Aljuaid A" first="Abdulelah" last="Aljuaid">Abdulelah Aljuaid</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Seliga, Alecia M" sort="Seliga, Alecia M" uniqKey="Seliga A" first="Alecia M" last="Seliga">Alecia M. Seliga</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Soule, Geoff" sort="Soule, Geoff" uniqKey="Soule G" first="Geoff" last="Soule">Geoff Soule</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2</wicri:regionArea><wicri:noRegion>Manitoba R3E 3R2</wicri:noRegion></affiliation></author><author><name sortKey="Morrow, Matthew" sort="Morrow, Matthew" uniqKey="Morrow M" first="Matthew" last="Morrow">Matthew Morrow</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kraynyak, Kimberly A" sort="Kraynyak, Kimberly A" uniqKey="Kraynyak K" first="Kimberly A" last="Kraynyak">Kimberly A. Kraynyak</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Khan, Amir S" sort="Khan, Amir S" uniqKey="Khan A" first="Amir S" last="Khan">Amir S. Khan</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Scott, Dana P" sort="Scott, Dana P" uniqKey="Scott D" first="Dana P" last="Scott">Dana P. Scott</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Lacasse, Rachel" sort="Lacasse, Rachel" uniqKey="Lacasse R" first="Rachel" last="Lacasse">Rachel Lacasse</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Meade White, Kimberly" sort="Meade White, Kimberly" uniqKey="Meade White K" first="Kimberly" last="Meade-White">Kimberly Meade-White</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Washington, Seattle, WA 98195, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Washington, Seattle, WA 98195</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Ugen, Kenneth E" sort="Ugen, Kenneth E" uniqKey="Ugen K" first="Kenneth E" last="Ugen">Kenneth E. Ugen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Sardesai, Niranjan Y" sort="Sardesai, Niranjan Y" uniqKey="Sardesai N" first="Niranjan Y" last="Sardesai">Niranjan Y. Sardesai</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kim, J Joseph" sort="Kim, J Joseph" uniqKey="Kim J" first="J Joseph" last="Kim">J Joseph Kim</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kobinger, Gary" sort="Kobinger, Gary" uniqKey="Kobinger G" first="Gary" last="Kobinger">Gary Kobinger</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2</wicri:regionArea><wicri:noRegion>Manitoba R3E 3R2</wicri:noRegion></affiliation></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Weiner, David B" sort="Weiner, David B" uniqKey="Weiner D" first="David B" last="Weiner">David B. Weiner</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA. dbweiner@mail.med.upenn.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26290414</idno><idno type="pmid">26290414</idno><idno type="doi">10.1126/scitranslmed.aac7462</idno><idno type="wicri:Area/PubMed/Corpus">001502</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001502</idno><idno type="wicri:Area/PubMed/Curation">001502</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001502</idno><idno type="wicri:Area/PubMed/Checkpoint">001654</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001654</idno><idno type="wicri:Area/Ncbi/Merge">001236</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.</title><author><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Reuschel, Emma L" sort="Reuschel, Emma L" uniqKey="Reuschel E" first="Emma L" last="Reuschel">Emma L. Reuschel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Tingey, Colleen" sort="Tingey, Colleen" uniqKey="Tingey C" first="Colleen" last="Tingey">Colleen Tingey</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Flingai, Seleeke" sort="Flingai, Seleeke" uniqKey="Flingai S" first="Seleeke" last="Flingai">Seleeke Flingai</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Villarreal, Daniel O" sort="Villarreal, Daniel O" uniqKey="Villarreal D" first="Daniel O" last="Villarreal">Daniel O. Villarreal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Wise, Megan" sort="Wise, Megan" uniqKey="Wise M" first="Megan" last="Wise">Megan Wise</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Patel, Ami" sort="Patel, Ami" uniqKey="Patel A" first="Ami" last="Patel">Ami Patel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Izmirly, Abdullah" sort="Izmirly, Abdullah" uniqKey="Izmirly A" first="Abdullah" last="Izmirly">Abdullah Izmirly</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Aljuaid, Abdulelah" sort="Aljuaid, Abdulelah" uniqKey="Aljuaid A" first="Abdulelah" last="Aljuaid">Abdulelah Aljuaid</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Seliga, Alecia M" sort="Seliga, Alecia M" uniqKey="Seliga A" first="Alecia M" last="Seliga">Alecia M. Seliga</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Soule, Geoff" sort="Soule, Geoff" uniqKey="Soule G" first="Geoff" last="Soule">Geoff Soule</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2</wicri:regionArea><wicri:noRegion>Manitoba R3E 3R2</wicri:noRegion></affiliation></author><author><name sortKey="Morrow, Matthew" sort="Morrow, Matthew" uniqKey="Morrow M" first="Matthew" last="Morrow">Matthew Morrow</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kraynyak, Kimberly A" sort="Kraynyak, Kimberly A" uniqKey="Kraynyak K" first="Kimberly A" last="Kraynyak">Kimberly A. Kraynyak</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Khan, Amir S" sort="Khan, Amir S" uniqKey="Khan A" first="Amir S" last="Khan">Amir S. Khan</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Scott, Dana P" sort="Scott, Dana P" uniqKey="Scott D" first="Dana P" last="Scott">Dana P. Scott</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Lacasse, Rachel" sort="Lacasse, Rachel" uniqKey="Lacasse R" first="Rachel" last="Lacasse">Rachel Lacasse</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Meade White, Kimberly" sort="Meade White, Kimberly" uniqKey="Meade White K" first="Kimberly" last="Meade-White">Kimberly Meade-White</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Washington, Seattle, WA 98195, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Washington, Seattle, WA 98195</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Ugen, Kenneth E" sort="Ugen, Kenneth E" uniqKey="Ugen K" first="Kenneth E" last="Ugen">Kenneth E. Ugen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Sardesai, Niranjan Y" sort="Sardesai, Niranjan Y" uniqKey="Sardesai N" first="Niranjan Y" last="Sardesai">Niranjan Y. Sardesai</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kim, J Joseph" sort="Kim, J Joseph" uniqKey="Kim J" first="J Joseph" last="Kim">J Joseph Kim</name><affiliation wicri:level="2"><nlm:affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Kobinger, Gary" sort="Kobinger, Gary" uniqKey="Kobinger G" first="Gary" last="Kobinger">Gary Kobinger</name><affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2</wicri:regionArea><wicri:noRegion>Manitoba R3E 3R2</wicri:noRegion></affiliation></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Weiner, David B" sort="Weiner, David B" uniqKey="Weiner D" first="David B" last="Weiner">David B. Weiner</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA. dbweiner@mail.med.upenn.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></analytic><series><title level="j">Science translational medicine</title><idno type="eISSN">1946-6242</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Camelus</term><term>Macaca mulatta</term><term>Mice</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Vaccines, DNA (therapeutic use)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Macaca mulatta</term><term>Souris</term><term>Vaccins à ADN (usage thérapeutique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Macaca mulatta</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chameaux</term><term>Macaca mulatta</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">First identified in 2012, Middle East respiratory syndrome (MERS) is caused by an emerging human coronavirus, which is distinct from the severe acute respiratory syndrome coronavirus (SARS-CoV), and represents a novel member of the lineage C betacoronoviruses. Since its identification, MERS coronavirus (MERS-CoV) has been linked to more than 1372 infections manifesting with severe morbidity and, often, mortality (about 495 deaths) in the Arabian Peninsula, Europe, and, most recently, the United States. Human-to-human transmission has been documented, with nosocomial transmission appearing to be an important route of infection. The recent increase in cases of MERS in the Middle East coupled with the lack of approved antiviral therapies or vaccines to treat or prevent this infection are causes for concern. We report on the development of a synthetic DNA vaccine against MERS-CoV. An optimized DNA vaccine encoding the MERS spike protein induced potent cellular immunity and antigen-specific neutralizing antibodies in mice, macaques, and camels. Vaccinated rhesus macaques seroconverted rapidly and exhibited high levels of virus-neutralizing activity. Upon MERS viral challenge, all of the monkeys in the control-vaccinated group developed characteristic disease, including pneumonia. Vaccinated macaques were protected and failed to demonstrate any clinical or radiographic signs of pneumonia. These studies demonstrate that a consensus MERS spike protein synthetic DNA vaccine can induce protective responses against viral challenge, indicating that this strategy may have value as a possible vaccine modality against this emerging pathogen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26290414</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>17</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1946-6242</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>301</Issue><PubDate><Year>2015</Year><Month>Aug</Month><Day>19</Day></PubDate></JournalIssue><Title>Science translational medicine</Title><ISOAbbreviation>Sci Transl Med</ISOAbbreviation></Journal><ArticleTitle>A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.</ArticleTitle><Pagination><MedlinePgn>301ra132</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1126/scitranslmed.aac7462</ELocationID><Abstract><AbstractText>First identified in 2012, Middle East respiratory syndrome (MERS) is caused by an emerging human coronavirus, which is distinct from the severe acute respiratory syndrome coronavirus (SARS-CoV), and represents a novel member of the lineage C betacoronoviruses. Since its identification, MERS coronavirus (MERS-CoV) has been linked to more than 1372 infections manifesting with severe morbidity and, often, mortality (about 495 deaths) in the Arabian Peninsula, Europe, and, most recently, the United States. Human-to-human transmission has been documented, with nosocomial transmission appearing to be an important route of infection. The recent increase in cases of MERS in the Middle East coupled with the lack of approved antiviral therapies or vaccines to treat or prevent this infection are causes for concern. We report on the development of a synthetic DNA vaccine against MERS-CoV. An optimized DNA vaccine encoding the MERS spike protein induced potent cellular immunity and antigen-specific neutralizing antibodies in mice, macaques, and camels. Vaccinated rhesus macaques seroconverted rapidly and exhibited high levels of virus-neutralizing activity. Upon MERS viral challenge, all of the monkeys in the control-vaccinated group developed characteristic disease, including pneumonia. Vaccinated macaques were protected and failed to demonstrate any clinical or radiographic signs of pneumonia. These studies demonstrate that a consensus MERS spike protein synthetic DNA vaccine can induce protective responses against viral challenge, indicating that this strategy may have value as a possible vaccine modality against this emerging pathogen.</AbstractText><CopyrightInformation>Copyright © 2015, American Association for the Advancement of Science.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Muthumani</LastName><ForeName>Karuppiah</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Falzarano</LastName><ForeName>Darryl</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reuschel</LastName><ForeName>Emma L</ForeName><Initials>EL</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tingey</LastName><ForeName>Colleen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Flingai</LastName><ForeName>Seleeke</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Villarreal</LastName><ForeName>Daniel O</ForeName><Initials>DO</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wise</LastName><ForeName>Megan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Ami</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Izmirly</LastName><ForeName>Abdullah</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aljuaid</LastName><ForeName>Abdulelah</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seliga</LastName><ForeName>Alecia M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soule</LastName><ForeName>Geoff</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morrow</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kraynyak</LastName><ForeName>Kimberly A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Amir S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scott</LastName><ForeName>Dana P</ForeName><Initials>DP</Initials><AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feldmann</LastName><ForeName>Friederike</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>LaCasse</LastName><ForeName>Rachel</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meade-White</LastName><ForeName>Kimberly</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, NIAID, NIH, Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Okumura</LastName><ForeName>Atsushi</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Microbiology, University of Washington, Seattle, WA 98195, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ugen</LastName><ForeName>Kenneth E</ForeName><Initials>KE</Initials><AffiliationInfo><Affiliation>Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sardesai</LastName><ForeName>Niranjan Y</ForeName><Initials>NY</Initials><AffiliationInfo><Affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>J Joseph</ForeName><Initials>JJ</Initials><AffiliationInfo><Affiliation>Inovio Pharmaceuticals Inc., Plymouth Meeting, PA 19462, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kobinger</LastName><ForeName>Gary</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feldmann</LastName><ForeName>Heinz</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weiner</LastName><ForeName>David B</ForeName><Initials>DB</Initials><AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA. dbweiner@mail.med.upenn.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 AI092843</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01-AI092843</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><Agency>Intramural NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Sci Transl Med</MedlineTA><NlmUniqueID>101505086</NlmUniqueID><ISSNLinking>1946-6234</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Nat Rev Drug Discov. 2015 Oct;14(10):678</RefSource><PMID Version="1">26424355</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Ann Transl Med. 2016 Dec;4(24):499</RefSource><PMID Version="1">28149861</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002162" MajorTopicYN="N">Camelus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000627" MajorTopicYN="Y">therapeutic use</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26290414</ArticleId><ArticleId IdType="pii">7/301/301ra132</ArticleId><ArticleId IdType="doi">10.1126/scitranslmed.aac7462</ArticleId><ArticleId IdType="pmc">PMC4573558</ArticleId><ArticleId IdType="mid">NIHMS720676</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nature. 2004 Apr 1;428(6982):561-4</Citation><ArticleIdList><ArticleId IdType="pubmed">15024391</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2009;485:395-405</Citation><ArticleIdList><ArticleId IdType="pubmed">19020839</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 May;83(9):4624-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19211745</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2011 Sep 9;29(39):6755-62</Citation><ArticleIdList><ArticleId IdType="pubmed">21238574</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Negl Trop Dis. 2011 Jan 11;5(1):e928</Citation><ArticleIdList><ArticleId IdType="pubmed">21264351</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2012 Aug 15;4(147):147ra114</Citation><ArticleIdList><ArticleId IdType="pubmed">22896678</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2012 Oct 10;4(155):155ra138</Citation><ArticleIdList><ArticleId IdType="pubmed">23052295</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012 Dec 11;3(6):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23232719</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(12):e52165</Citation><ArticleIdList><ArticleId IdType="pubmed">23284919</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2013 Jan 15;4(1):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23322635</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2013 Aug;67(2):130-40</Citation><ArticleIdList><ArticleId IdType="pubmed">23583636</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2013 Apr 30;4(3):e00165-13</Citation><ArticleIdList><ArticleId IdType="pubmed">23631916</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ther. 2013 Jul;21(7):1432-44</Citation><ArticleIdList><ArticleId IdType="pubmed">23670573</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Jun 27;368(26):2487-94</Citation><ArticleIdList><ArticleId IdType="pubmed">23718156</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Aug;87(15):8638-50</Citation><ArticleIdList><ArticleId IdType="pubmed">23720729</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2013 Jun 15;381(9883):2069</Citation><ArticleIdList><ArticleId IdType="pubmed">23776959</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Aug;87(16):9379-83</Citation><ArticleIdList><ArticleId IdType="pubmed">23785207</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2013 Jun 29;381(9885):2229-30</Citation><ArticleIdList><ArticleId IdType="pubmed">23809548</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation><ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Aug 29;369(9):884-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23923992</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2013 Aug 20;4(4):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23963179</ArticleId></ArticleIdList></Reference><Reference><Citation>J Thorac Dis. 2013 Aug;5 Suppl 2:S103-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23977429</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2013 Jul;13(7):573-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23977673</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2013 Aug 26;10:266</Citation><ArticleIdList><ArticleId IdType="pubmed">23978242</ArticleId></ArticleIdList></Reference><Reference><Citation>Antiviral Res. 2013 Oct;100(1):286-95</Citation><ArticleIdList><ArticleId IdType="pubmed">24012996</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2013 Oct;19(10):1313-7</Citation><ArticleIdList><ArticleId IdType="pubmed">24013700</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013;9(9):e1003595</Citation><ArticleIdList><ArticleId IdType="pubmed">24039577</ArticleId></ArticleIdList></Reference><Reference><Citation>Hum Vaccin Immunother. 2013 Oct;9(10):2253-62</Citation><ArticleIdList><ArticleId IdType="pubmed">24045230</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Sep 19;501(7467):294-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24048048</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2013 Dec 14;382(9909):1993-2002</Citation><ArticleIdList><ArticleId IdType="pubmed">24055451</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16598-603</Citation><ArticleIdList><ArticleId IdType="pubmed">24062443</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Sep 05;18(36):pii=20574</Citation><ArticleIdList><ArticleId IdType="pubmed">24079378</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Sep 19;18(38):null</Citation><ArticleIdList><ArticleId IdType="pubmed">24084338</ArticleId></ArticleIdList></Reference><Reference><Citation>Expert Rev Anti Infect Ther. 2013 Oct;11(10):979-81</Citation><ArticleIdList><ArticleId IdType="pubmed">24093587</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2013 Dec;67(6):606-16</Citation><ArticleIdList><ArticleId IdType="pubmed">24096239</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Oct 03;8(10):e76469</Citation><ArticleIdList><ArticleId IdType="pubmed">24098509</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Oct 31;369(18):1761-2</Citation><ArticleIdList><ArticleId IdType="pubmed">24171524</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2013 Dec;11(12):836-48</Citation><ArticleIdList><ArticleId IdType="pubmed">24217413</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Gene Ther. 2013 Dec;20(12):652-62</Citation><ArticleIdList><ArticleId IdType="pubmed">24310062</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Dec 31;8(12):e84234</Citation><ArticleIdList><ArticleId IdType="pubmed">24391921</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 2014 Mar 15;74(6):1789-800</Citation><ArticleIdList><ArticleId IdType="pubmed">24448242</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):100-1</Citation><ArticleIdList><ArticleId IdType="pubmed">24457167</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2014 Feb 18;5(1):null</Citation><ArticleIdList><ArticleId IdType="pubmed">24549846</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 May;88(10):5209-12</Citation><ArticleIdList><ArticleId IdType="pubmed">24600003</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2018-26</Citation><ArticleIdList><ArticleId IdType="pubmed">24778221</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2014 Apr 30;6(234):234ra59</Citation><ArticleIdList><ArticleId IdType="pubmed">24778414</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Jul;88(14):7796-805</Citation><ArticleIdList><ArticleId IdType="pubmed">24789777</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2014 May 7;6(235):235fs19</Citation><ArticleIdList><ArticleId IdType="pubmed">24807556</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2014 Aug 21;10(8):e1004250</Citation><ArticleIdList><ArticleId IdType="pubmed">25144235</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Infect Dis. 2014 Dec;29:307-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25448335</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2015 Sep 5;386(9997):995-1007</Citation><ArticleIdList><ArticleId IdType="pubmed">26049252</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol Methods. 1998 Dec 1;221(1-2):35-41</Citation><ArticleIdList><ArticleId IdType="pubmed">9894896</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Floride</li><li>Montana</li><li>Pennsylvanie</li><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Muthumani, Karuppiah" sort="Muthumani, Karuppiah" uniqKey="Muthumani K" first="Karuppiah" last="Muthumani">Karuppiah Muthumani</name></region><name sortKey="Aljuaid, Abdulelah" sort="Aljuaid, Abdulelah" uniqKey="Aljuaid A" first="Abdulelah" last="Aljuaid">Abdulelah Aljuaid</name><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Flingai, Seleeke" sort="Flingai, Seleeke" uniqKey="Flingai S" first="Seleeke" last="Flingai">Seleeke Flingai</name><name sortKey="Izmirly, Abdullah" sort="Izmirly, Abdullah" uniqKey="Izmirly A" first="Abdullah" last="Izmirly">Abdullah Izmirly</name><name sortKey="Khan, Amir S" sort="Khan, Amir S" uniqKey="Khan A" first="Amir S" last="Khan">Amir S. Khan</name><name sortKey="Kim, J Joseph" sort="Kim, J Joseph" uniqKey="Kim J" first="J Joseph" last="Kim">J Joseph Kim</name><name sortKey="Kraynyak, Kimberly A" sort="Kraynyak, Kimberly A" uniqKey="Kraynyak K" first="Kimberly A" last="Kraynyak">Kimberly A. Kraynyak</name><name sortKey="Lacasse, Rachel" sort="Lacasse, Rachel" uniqKey="Lacasse R" first="Rachel" last="Lacasse">Rachel Lacasse</name><name sortKey="Meade White, Kimberly" sort="Meade White, Kimberly" uniqKey="Meade White K" first="Kimberly" last="Meade-White">Kimberly Meade-White</name><name sortKey="Morrow, Matthew" sort="Morrow, Matthew" uniqKey="Morrow M" first="Matthew" last="Morrow">Matthew Morrow</name><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><name sortKey="Patel, Ami" sort="Patel, Ami" uniqKey="Patel A" first="Ami" last="Patel">Ami Patel</name><name sortKey="Reuschel, Emma L" sort="Reuschel, Emma L" uniqKey="Reuschel E" first="Emma L" last="Reuschel">Emma L. Reuschel</name><name sortKey="Sardesai, Niranjan Y" sort="Sardesai, Niranjan Y" uniqKey="Sardesai N" first="Niranjan Y" last="Sardesai">Niranjan Y. Sardesai</name><name sortKey="Scott, Dana P" sort="Scott, Dana P" uniqKey="Scott D" first="Dana P" last="Scott">Dana P. Scott</name><name sortKey="Seliga, Alecia M" sort="Seliga, Alecia M" uniqKey="Seliga A" first="Alecia M" last="Seliga">Alecia M. Seliga</name><name sortKey="Tingey, Colleen" sort="Tingey, Colleen" uniqKey="Tingey C" first="Colleen" last="Tingey">Colleen Tingey</name><name sortKey="Ugen, Kenneth E" sort="Ugen, Kenneth E" uniqKey="Ugen K" first="Kenneth E" last="Ugen">Kenneth E. Ugen</name><name sortKey="Villarreal, Daniel O" sort="Villarreal, Daniel O" uniqKey="Villarreal D" first="Daniel O" last="Villarreal">Daniel O. Villarreal</name><name sortKey="Weiner, David B" sort="Weiner, David B" uniqKey="Weiner D" first="David B" last="Weiner">David B. Weiner</name><name sortKey="Wise, Megan" sort="Wise, Megan" uniqKey="Wise M" first="Megan" last="Wise">Megan Wise</name></country><country name="Canada"><noRegion><name sortKey="Soule, Geoff" sort="Soule, Geoff" uniqKey="Soule G" first="Geoff" last="Soule">Geoff Soule</name></noRegion><name sortKey="Kobinger, Gary" sort="Kobinger, Gary" uniqKey="Kobinger G" first="Gary" last="Kobinger">Gary Kobinger</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001236 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001236 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:26290414
|texte= A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:26290414" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |