MersV1, Ncbi, Merge, bibRecord, 000E24

[A novel one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses].

Identifieur interne : 000E24 ( Ncbi/Merge ); précédent : 000E23; suivant : 000E25

[A novel one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses].

Auteurs : Peihua Niu [République populaire de Chine] ; Chen Zhang ; Roujian Lu ; Ji Wang ; Yongliang Lou ; Wenjie Tan [Oman] ; Xuejun Ma [République populaire de Chine]

Source :

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 2014.

RBID : pubmed:24985384

Descripteurs français

English descriptors

KwdEn :
- Coronavirus, Humans, Multiplex Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction (methods), Reproducibility of Results, Sensitivity and Specificity.
MESH :
- methods : Real-Time Polymerase Chain Reaction.
- Coronavirus, Humans, Multiplex Polymerase Chain Reaction, Reproducibility of Results, Sensitivity and Specificity.

Abstract

To develop an one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses (HCoVs).

PubMed: 24985384

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pubmed:24985384

Le document en format XML

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<author><name sortKey="Niu, Peihua" sort="Niu, Peihua" uniqKey="Niu P" first="Peihua" last="Niu">Peihua Niu</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325035, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325035</wicri:regionArea>
<wicri:noRegion>Wenzhou 325035</wicri:noRegion>
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<author><name sortKey="Zhang, Chen" sort="Zhang, Chen" uniqKey="Zhang C" first="Chen" last="Zhang">Chen Zhang</name>
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<author><name sortKey="Lu, Roujian" sort="Lu, Roujian" uniqKey="Lu R" first="Roujian" last="Lu">Roujian Lu</name>
</author>
<author><name sortKey="Wang, Ji" sort="Wang, Ji" uniqKey="Wang J" first="Ji" last="Wang">Ji Wang</name>
</author>
<author><name sortKey="Lou, Yongliang" sort="Lou, Yongliang" uniqKey="Lou Y" first="Yongliang" last="Lou">Yongliang Lou</name>
</author>
<author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name>
<affiliation wicri:level="1"><nlm:affiliation>Email: tanwj@hotmail.com.</nlm:affiliation>
<country wicri:rule="url">Oman</country>
</affiliation>
</author>
<author><name sortKey="Ma, Xuejun" sort="Ma, Xuejun" uniqKey="Ma X" first="Xuejun" last="Ma">Xuejun Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>Email: maxj@ivdc.chinacdc.cn.</nlm:affiliation>
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<affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325035, China.</nlm:affiliation>
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<author><name sortKey="Wang, Ji" sort="Wang, Ji" uniqKey="Wang J" first="Ji" last="Wang">Ji Wang</name>
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<author><name sortKey="Lou, Yongliang" sort="Lou, Yongliang" uniqKey="Lou Y" first="Yongliang" last="Lou">Yongliang Lou</name>
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<author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name>
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<author><name sortKey="Ma, Xuejun" sort="Ma, Xuejun" uniqKey="Ma X" first="Xuejun" last="Ma">Xuejun Ma</name>
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<country wicri:rule="url">République populaire de Chine</country>
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<series><title level="j">Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</title>
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<term>Humans</term>
<term>Multiplex Polymerase Chain Reaction</term>
<term>Real-Time Polymerase Chain Reaction (methods)</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Coronavirus</term>
<term>Humains</term>
<term>Reproductibilité des résultats</term>
<term>Réaction de polymérisation en chaine en temps réel ()</term>
<term>Réaction de polymérisation en chaine multiplex</term>
<term>Sensibilité et spécificité</term>
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<term>Humans</term>
<term>Multiplex Polymerase Chain Reaction</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Coronavirus</term>
<term>Humains</term>
<term>Reproductibilité des résultats</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Réaction de polymérisation en chaine multiplex</term>
<term>Sensibilité et spécificité</term>
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<front><div type="abstract" xml:lang="en">To develop an one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses (HCoVs).</div>
</front>
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<DateCompleted><Year>2015</Year>
<Month>06</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2014</Year>
<Month>07</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9624</ISSN>
<JournalIssue CitedMedium="Print"><Volume>48</Volume>
<Issue>5</Issue>
<PubDate><Year>2014</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</Title>
<ISOAbbreviation>Zhonghua Yu Fang Yi Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[A novel one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses].</ArticleTitle>
<Pagination><MedlinePgn>416-9</MedlinePgn>
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<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop an one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses (HCoVs).</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Genbank sequences of six human coronaviruses, including HCoV-NL63, HCoV-229E, SARS-CoV, HCoV-OC43, MERS-CoV, and HCoV-HKU1, were included as reference sequences. Primers were designed based on multiple alignment of reference sequences, targeting the conserved regions of each species of HcoV. Virus strains and nucleic acid preserved in our lab were used as template in developing this automatic-electrophoresis-based one-tube multiplex RT-PCR assay. Detection limits and reproducibility were also evaluated with these templates. Samples with infection of other respiratory viruses preserved in our lab were used to evaluate specificity of this assay. Finally, we tested this assay with 140 clinical samples that were validated by real-time PCR in parallel.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">This automatic-electrophoresis-based multiplex RT-PCR assay was able to detect six human coronaviruses simultaneously. All positive samples in this study were detected with at least one specific fragment of anticipated length (195, 304, 332, 378, 415, 442 bp) . No fragment was detected in negative controls. Detection limits of 1.0×10(1-1.0)×10(2) copies/µl were achieved in tests of single virus. No cross reaction was observed with other respiratory viruses. This multiplex RT-PCR assay showed the same sensitivity and specificity to that of individual real-time RT-PCR validated with clinical samples. Both methods detected 28 positive samples (20%) .</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Six HCoVs can be detected in one tube by this novel multiplex RT-PCR assay with high sensitivity and specificity.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Niu</LastName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhonghua Yu Fang Yi Xue Za Zhi</MedlineTA>
<NlmUniqueID>7904962</NlmUniqueID>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="Y">Coronavirus</DescriptorName>
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<name sortKey="Wang, Ji" sort="Wang, Ji" uniqKey="Wang J" first="Ji" last="Wang">Ji Wang</name>
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[A novel one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses].

[A novel one-tube multiplex RT-PCR assay for simultaneous detection of six human coronaviruses].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki