MersV1, Ncbi, Merge, bibRecord, 000C77

Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

Identifieur interne : 000C77 ( Ncbi/Merge ); précédent : 000C76; suivant : 000C78

Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

Auteurs : M. Koizumi [Japon] ; S. Iwai ; E. Ohtsuka

Source :

FEBS letters [ 0014-5793 ] ; 1988.

RBID : pubmed:2449365

Descripteurs français

KwdFr :
- ADN satellite (génétique), ARN (métabolisme), ARN (synthèse chimique), ARN double brin (métabolisme), ARN double brin (synthèse chimique), Animaux, Composition en bases nucléiques, Estérification, Magnésium (pharmacologie), Polyribonucléotides, Salamandridae (génétique), Séquence nucléotidique, Transcription génétique.
MESH :
- génétique : ADN satellite, Salamandridae.
- métabolisme : ARN, ARN double brin.
- pharmacologie : Magnésium.
- synthèse chimique : ARN, ARN double brin.
- Animaux, Composition en bases nucléiques, Estérification, Polyribonucléotides, Séquence nucléotidique, Transcription génétique.

English descriptors

KwdEn :
- Animals, Base Composition, Base Sequence, DNA, Satellite (genetics), Esterification, Magnesium (pharmacology), Polyribonucleotides, RNA (chemical synthesis), RNA (metabolism), RNA, Double-Stranded (chemical synthesis), RNA, Double-Stranded (metabolism), Salamandridae (genetics), Transcription, Genetic.
MESH :
- chemical , chemical synthesis : RNA, RNA, Double-Stranded.
- chemical , genetics : DNA, Satellite.
- chemical , metabolism : RNA, RNA, Double-Stranded.
- chemical , pharmacology : Magnesium.
- genetics : Salamandridae.
- Animals, Base Composition, Base Sequence, Esterification, Polyribonucleotides, Transcription, Genetic.

Abstract

Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3'-terminal 2',3'-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3'-phosphate was not susceptible to transesterification at the cleavage site.

DOI: 10.1016/0014-5793(88)80004-8
PubMed: 2449365

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pubmed:2449365

Le document en format XML

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<author><name sortKey="Koizumi, M" sort="Koizumi, M" uniqKey="Koizumi M" first="M" last="Koizumi">M. Koizumi</name>
<affiliation wicri:level="1"><nlm:affiliation>Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo</wicri:regionArea>
<wicri:noRegion>Sapporo</wicri:noRegion>
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<author><name sortKey="Iwai, S" sort="Iwai, S" uniqKey="Iwai S" first="S" last="Iwai">S. Iwai</name>
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<author><name sortKey="Ohtsuka, E" sort="Ohtsuka, E" uniqKey="Ohtsuka E" first="E" last="Ohtsuka">E. Ohtsuka</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Base Composition</term>
<term>Base Sequence</term>
<term>DNA, Satellite (genetics)</term>
<term>Esterification</term>
<term>Magnesium (pharmacology)</term>
<term>Polyribonucleotides</term>
<term>RNA (chemical synthesis)</term>
<term>RNA (metabolism)</term>
<term>RNA, Double-Stranded (chemical synthesis)</term>
<term>RNA, Double-Stranded (metabolism)</term>
<term>Salamandridae (genetics)</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN satellite (génétique)</term>
<term>ARN (métabolisme)</term>
<term>ARN (synthèse chimique)</term>
<term>ARN double brin (métabolisme)</term>
<term>ARN double brin (synthèse chimique)</term>
<term>Animaux</term>
<term>Composition en bases nucléiques</term>
<term>Estérification</term>
<term>Magnésium (pharmacologie)</term>
<term>Polyribonucléotides</term>
<term>Salamandridae (génétique)</term>
<term>Séquence nucléotidique</term>
<term>Transcription génétique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>RNA</term>
<term>RNA, Double-Stranded</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Satellite</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA</term>
<term>RNA, Double-Stranded</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Magnesium</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Salamandridae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN satellite</term>
<term>Salamandridae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN</term>
<term>ARN double brin</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Magnésium</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>ARN</term>
<term>ARN double brin</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Base Composition</term>
<term>Base Sequence</term>
<term>Esterification</term>
<term>Polyribonucleotides</term>
<term>Transcription, Genetic</term>
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<term>Composition en bases nucléiques</term>
<term>Estérification</term>
<term>Polyribonucléotides</term>
<term>Séquence nucléotidique</term>
<term>Transcription génétique</term>
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<front><div type="abstract" xml:lang="en">Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3'-terminal 2',3'-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3'-phosphate was not susceptible to transesterification at the cleavage site.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2449365</PMID>
<DateCompleted><Year>1988</Year>
<Month>04</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>06</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-5793</ISSN>
<JournalIssue CitedMedium="Print"><Volume>228</Volume>
<Issue>2</Issue>
<PubDate><Year>1988</Year>
<Month>Feb</Month>
<Day>15</Day>
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<Title>FEBS letters</Title>
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<ArticleTitle>Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.</ArticleTitle>
<Pagination><MedlinePgn>228-30</MedlinePgn>
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<Abstract><AbstractText>Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3'-terminal 2',3'-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3'-phosphate was not susceptible to transesterification at the cleavage site.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koizumi</LastName>
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<AffiliationInfo><Affiliation>Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.</Affiliation>
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<Author ValidYN="Y"><LastName>Iwai</LastName>
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<Author ValidYN="Y"><LastName>Ohtsuka</LastName>
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<MeshHeading><DescriptorName UI="D008274" MajorTopicYN="N">Magnesium</DescriptorName>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki