Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.
Identifieur interne : 000736 ( Ncbi/Merge ); précédent : 000735; suivant : 000737Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.
Auteurs : Onnop Srivannavit [États-Unis] ; Mayurachat Gulari ; Zhishan Hua ; Xiaolian Gao ; Xiaochuan Zhou ; Ailing Hong ; Tiecheng Zhou ; Erdogan GulariSource :
- Sensors and actuators. B, Chemical [ 0925-4005 ] ; 2009.
Abstract
We have designed and fabricated a microfluidic reactor array device for massively parallel in-situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in-situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98 %. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.
DOI: 10.1016/j.snb.2009.04.071
PubMed: 20161215
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001F67
- to stream PubMed, to step Curation: 001F67
- to stream PubMed, to step Checkpoint: 001E99
Links to Exploration step
pubmed:20161215Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.</title>
<author><name sortKey="Srivannavit, Onnop" sort="Srivannavit, Onnop" uniqKey="Srivannavit O" first="Onnop" last="Srivannavit">Onnop Srivannavit</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gulari, Mayurachat" sort="Gulari, Mayurachat" uniqKey="Gulari M" first="Mayurachat" last="Gulari">Mayurachat Gulari</name>
</author>
<author><name sortKey="Hua, Zhishan" sort="Hua, Zhishan" uniqKey="Hua Z" first="Zhishan" last="Hua">Zhishan Hua</name>
</author>
<author><name sortKey="Gao, Xiaolian" sort="Gao, Xiaolian" uniqKey="Gao X" first="Xiaolian" last="Gao">Xiaolian Gao</name>
</author>
<author><name sortKey="Zhou, Xiaochuan" sort="Zhou, Xiaochuan" uniqKey="Zhou X" first="Xiaochuan" last="Zhou">Xiaochuan Zhou</name>
</author>
<author><name sortKey="Hong, Ailing" sort="Hong, Ailing" uniqKey="Hong A" first="Ailing" last="Hong">Ailing Hong</name>
</author>
<author><name sortKey="Zhou, Tiecheng" sort="Zhou, Tiecheng" uniqKey="Zhou T" first="Tiecheng" last="Zhou">Tiecheng Zhou</name>
</author>
<author><name sortKey="Gulari, Erdogan" sort="Gulari, Erdogan" uniqKey="Gulari E" first="Erdogan" last="Gulari">Erdogan Gulari</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:20161215</idno>
<idno type="pmid">20161215</idno>
<idno type="doi">10.1016/j.snb.2009.04.071</idno>
<idno type="wicri:Area/PubMed/Corpus">001F67</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F67</idno>
<idno type="wicri:Area/PubMed/Curation">001F67</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F67</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001E99</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E99</idno>
<idno type="wicri:Area/Ncbi/Merge">000736</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.</title>
<author><name sortKey="Srivannavit, Onnop" sort="Srivannavit, Onnop" uniqKey="Srivannavit O" first="Onnop" last="Srivannavit">Onnop Srivannavit</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gulari, Mayurachat" sort="Gulari, Mayurachat" uniqKey="Gulari M" first="Mayurachat" last="Gulari">Mayurachat Gulari</name>
</author>
<author><name sortKey="Hua, Zhishan" sort="Hua, Zhishan" uniqKey="Hua Z" first="Zhishan" last="Hua">Zhishan Hua</name>
</author>
<author><name sortKey="Gao, Xiaolian" sort="Gao, Xiaolian" uniqKey="Gao X" first="Xiaolian" last="Gao">Xiaolian Gao</name>
</author>
<author><name sortKey="Zhou, Xiaochuan" sort="Zhou, Xiaochuan" uniqKey="Zhou X" first="Xiaochuan" last="Zhou">Xiaochuan Zhou</name>
</author>
<author><name sortKey="Hong, Ailing" sort="Hong, Ailing" uniqKey="Hong A" first="Ailing" last="Hong">Ailing Hong</name>
</author>
<author><name sortKey="Zhou, Tiecheng" sort="Zhou, Tiecheng" uniqKey="Zhou T" first="Tiecheng" last="Zhou">Tiecheng Zhou</name>
</author>
<author><name sortKey="Gulari, Erdogan" sort="Gulari, Erdogan" uniqKey="Gulari E" first="Erdogan" last="Gulari">Erdogan Gulari</name>
</author>
</analytic>
<series><title level="j">Sensors and actuators. B, Chemical</title>
<idno type="ISSN">0925-4005</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have designed and fabricated a microfluidic reactor array device for massively parallel in-situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in-situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98 %. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">20161215</PMID>
<DateRevised><Year>2019</Year>
<Month>11</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0925-4005</ISSN>
<JournalIssue CitedMedium="Print"><Volume>140</Volume>
<Issue>2</Issue>
<PubDate><Year>2009</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Sensors and actuators. B, Chemical</Title>
<ISOAbbreviation>Sens Actuators B Chem</ISOAbbreviation>
</Journal>
<ArticleTitle>Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.</ArticleTitle>
<Pagination><MedlinePgn>473-481</MedlinePgn>
</Pagination>
<Abstract><AbstractText>We have designed and fabricated a microfluidic reactor array device for massively parallel in-situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in-situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98 %. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Srivannavit</LastName>
<ForeName>Onnop</ForeName>
<Initials>O</Initials>
<AffiliationInfo><Affiliation>Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gulari</LastName>
<ForeName>Mayurachat</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hua</LastName>
<ForeName>Zhishan</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>Xiaolian</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Xiaochuan</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hong</LastName>
<ForeName>Ailing</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Tiecheng</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gulari</LastName>
<ForeName>Erdogan</ForeName>
<Initials>E</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>R21 HG003725-01</GrantID>
<Acronym>HG</Acronym>
<Agency>NHGRI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R41 HG004103-01</GrantID>
<Acronym>HG</Acronym>
<Agency>NHGRI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Sens Actuators B Chem</MedlineTA>
<NlmUniqueID>101149755</NlmUniqueID>
<ISSNLinking>0925-4005</ISSNLinking>
</MedlineJournalInfo>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year>
<Month>2</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2010</Year>
<Month>2</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2010</Year>
<Month>2</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">20161215</ArticleId>
<ArticleId IdType="doi">10.1016/j.snb.2009.04.071</ArticleId>
<ArticleId IdType="pmc">PMC2716077</ArticleId>
<ArticleId IdType="mid">NIHMS119877</ArticleId>
</ArticleIdList>
<pmc-dir>nihms</pmc-dir>
<ReferenceList><Reference><Citation>Anal Chem. 2003 Sep 1;75(17):4672-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14632079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2001 Nov 15;29(22):4744-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11713325</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1999 Oct 15;27(20):4100-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10497276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2002 Nov;12(11):1749-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12421762</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comb Chem. 2000 Jul-Aug;2(4):349-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10891102</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2005 May 03;33(8):e75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15870385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1991 Feb 15;251(4995):767-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1990438</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2008 Jan;36(1):e7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18084027</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2003 Dec 1;31(23):e151</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14627841</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 1999 Oct;17(10):974-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10504697</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004 Oct 11;32(18):5409-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15477391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biotechnol. 2006 Jun 25;124(1):206-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16436303</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2000 Nov 15;28(22):4552-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11071945</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2005 Aug 05;33(14):e125</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16085751</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Dec 23;432(7020):1050-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15616567</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13555-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8942972</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2004;5(8):R58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15287980</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomol Eng. 2006 Oct;23(5):213-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16905357</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Michigan</li>
</region>
</list>
<tree><noCountry><name sortKey="Gao, Xiaolian" sort="Gao, Xiaolian" uniqKey="Gao X" first="Xiaolian" last="Gao">Xiaolian Gao</name>
<name sortKey="Gulari, Erdogan" sort="Gulari, Erdogan" uniqKey="Gulari E" first="Erdogan" last="Gulari">Erdogan Gulari</name>
<name sortKey="Gulari, Mayurachat" sort="Gulari, Mayurachat" uniqKey="Gulari M" first="Mayurachat" last="Gulari">Mayurachat Gulari</name>
<name sortKey="Hong, Ailing" sort="Hong, Ailing" uniqKey="Hong A" first="Ailing" last="Hong">Ailing Hong</name>
<name sortKey="Hua, Zhishan" sort="Hua, Zhishan" uniqKey="Hua Z" first="Zhishan" last="Hua">Zhishan Hua</name>
<name sortKey="Zhou, Tiecheng" sort="Zhou, Tiecheng" uniqKey="Zhou T" first="Tiecheng" last="Zhou">Tiecheng Zhou</name>
<name sortKey="Zhou, Xiaochuan" sort="Zhou, Xiaochuan" uniqKey="Zhou X" first="Xiaochuan" last="Zhou">Xiaochuan Zhou</name>
</noCountry>
<country name="États-Unis"><region name="Michigan"><name sortKey="Srivannavit, Onnop" sort="Srivannavit, Onnop" uniqKey="Srivannavit O" first="Onnop" last="Srivannavit">Onnop Srivannavit</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000736 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000736 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:20161215 |texte= Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:20161215" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |