Quadruple 9-mer-based protein binding microarray with DsRed fusion protein
Identifieur interne : 000713 ( Ncbi/Merge ); précédent : 000712; suivant : 000714Quadruple 9-mer-based protein binding microarray with DsRed fusion protein
Auteurs : Min-Jeong Kim [Corée du Sud] ; Tae-Ho Lee [Corée du Sud] ; Yoon-Mok Pahk [Corée du Sud] ; Yul-Ho Kim [Corée du Sud] ; Hyang-Mi Park [Corée du Sud] ; Yang Do Choi [Corée du Sud] ; Baek Hie Nahm [Corée du Sud] ; Yeon-Ki Kim [Corée du Sud]Source :
- BMC Molecular Biology [ 1471-2199 ] ; 2009.
Descripteurs français
- KwdFr :
- Analyse par réseau de protéines (), Liaison aux protéines, Motifs d'acides aminés, Oligonucléotides (génétique), Oligonucléotides (métabolisme), Oryza (), Oryza (génétique), Oryza (métabolisme), Protéines luminescentes (génétique), Protéines luminescentes (métabolisme), Protéines végétales (), Protéines végétales (génétique), Protéines végétales (métabolisme), Sites de fixation.
- MESH :
English descriptors
- KwdEn :
- Amino Acid Motifs, Binding Sites, Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Oligonucleotides (genetics), Oligonucleotides (metabolism), Oryza (chemistry), Oryza (genetics), Oryza (metabolism), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Array Analysis (methods), Protein Binding.
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Luminescent Proteins, Oligonucleotides, Plant Proteins.
- chemical , metabolism : Luminescent Proteins, Oligonucleotides, Plant Proteins.
- chemistry : Oryza.
- genetics : Oryza.
- metabolism : Oryza.
- methods : Protein Array Analysis.
- Amino Acid Motifs, Binding Sites, Protein Binding.
Abstract
The interaction between a transcription factor and DNA motif (
We facilitated the PBM technology using a DsRed fluorescent protein and a concatenated sequence of oligonucleotides. The PBM was designed in such a way that target probes were synthesized as quadruples of all possible 9-mer combinations, permitting unequivocal interpretation of the
Our method demonstrated PBM can be conveniently performed by adopting: (1) quadruple 9-mers may increase protein-DNA binding interactions in the microarray, and (2) a one-step incubation shortens the wash and hybridization steps. This technology will facilitate greater understanding of genome-wide interactions between proteins and DNA.
Url:
DOI: 10.1186/1471-2199-10-91
PubMed: 19761621
PubMed Central: 2754467
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000554
- to stream Pmc, to step Curation: 000554
- to stream Pmc, to step Checkpoint: 001369
- to stream PubMed, to step Corpus: 001F92
- to stream PubMed, to step Curation: 001F92
- to stream PubMed, to step Checkpoint: 001E84
Links to Exploration step
PMC:2754467Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19761621</idno>
<idno type="pmc">2754467</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754467</idno>
<idno type="RBID">PMC:2754467</idno>
<idno type="doi">10.1186/1471-2199-10-91</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">000554</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000554</idno>
<idno type="wicri:Area/Pmc/Curation">000554</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000554</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001369</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001369</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:19761621</idno>
<idno type="wicri:Area/PubMed/Corpus">001F92</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F92</idno>
<idno type="wicri:Area/PubMed/Curation">001F92</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F92</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001E84</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E84</idno>
<idno type="wicri:Area/Ncbi/Merge">000713</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Molecular Biology</title>
<idno type="eISSN">1471-2199</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Binding Sites</term>
<term>Luminescent Proteins (genetics)</term>
<term>Luminescent Proteins (metabolism)</term>
<term>Oligonucleotides (genetics)</term>
<term>Oligonucleotides (metabolism)</term>
<term>Oryza (chemistry)</term>
<term>Oryza (genetics)</term>
<term>Oryza (metabolism)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Protein Array Analysis (methods)</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse par réseau de protéines ()</term>
<term>Liaison aux protéines</term>
<term>Motifs d'acides aminés</term>
<term>Oligonucléotides (génétique)</term>
<term>Oligonucléotides (métabolisme)</term>
<term>Oryza ()</term>
<term>Oryza (génétique)</term>
<term>Oryza (métabolisme)</term>
<term>Protéines luminescentes (génétique)</term>
<term>Protéines luminescentes (métabolisme)</term>
<term>Protéines végétales ()</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Sites de fixation</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luminescent Proteins</term>
<term>Oligonucleotides</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Luminescent Proteins</term>
<term>Oligonucleotides</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oligonucléotides</term>
<term>Oryza</term>
<term>Protéines luminescentes</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Array Analysis</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Oligonucléotides</term>
<term>Oryza</term>
<term>Protéines luminescentes</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Binding Sites</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse par réseau de protéines</term>
<term>Liaison aux protéines</term>
<term>Motifs d'acides aminés</term>
<term>Oryza</term>
<term>Protéines végétales</term>
<term>Sites de fixation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The interaction between a transcription factor and DNA motif (<italic>cis</italic>
-acting element) is an important regulatory step in gene regulation. Comprehensive genome-wide methods have been developed to characterize protein-DNA interactions. Recently, the universal protein binding microarray (PBM) was introduced to determine if a DNA motif interacts with proteins in a genome-wide manner.</p>
</sec>
<sec><title>Results</title>
<p>We facilitated the PBM technology using a DsRed fluorescent protein and a concatenated sequence of oligonucleotides. The PBM was designed in such a way that target probes were synthesized as quadruples of all possible 9-mer combinations, permitting unequivocal interpretation of the <italic>cis</italic>
-acting elements. The complimentary DNA strands of the features were synthesized with a primer and DNA polymerase on microarray slides. Proteins were labeled via N-terminal fusion with DsRed fluorescent protein, which circumvents the need for a multi-step incubation. The PBM presented herein confirmed the well-known DNA binding sequences of Cbf1 and CBF1/DREB1B, and it was also applied to elucidate the unidentified <italic>cis</italic>
-acting element of the OsNAC6 rice transcription factor.</p>
</sec>
<sec><title>Conclusion</title>
<p>Our method demonstrated PBM can be conveniently performed by adopting: (1) quadruple 9-mers may increase protein-DNA binding interactions in the microarray, and (2) a one-step incubation shortens the wash and hybridization steps. This technology will facilitate greater understanding of genome-wide interactions between proteins and DNA.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<double pmid="19761621"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19761621</idno>
<idno type="pmc">2754467</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754467</idno>
<idno type="RBID">PMC:2754467</idno>
<idno type="doi">10.1186/1471-2199-10-91</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">000554</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000554</idno>
<idno type="wicri:Area/Pmc/Curation">000554</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000554</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001369</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001369</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
<affiliation wicri:level="1"><nlm:aff id="I4">National Institute of Crop Science, Suwon 441-857, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>National Institute of Crop Science, Suwon 441-857</wicri:regionArea>
<wicri:noRegion>Suwon 441-857</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
<affiliation wicri:level="4"><nlm:aff id="I1">Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
<orgName type="university">Université nationale de Séoul</orgName>
<placeName><settlement type="city">Séoul</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I3">Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
<affiliation wicri:level="1"><nlm:aff id="I2">GreenGene Biotech Inc., Myongji University, Yongin 449-728, Korea</nlm:aff>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc., Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Molecular Biology</title>
<idno type="eISSN">1471-2199</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The interaction between a transcription factor and DNA motif (<italic>cis</italic>
-acting element) is an important regulatory step in gene regulation. Comprehensive genome-wide methods have been developed to characterize protein-DNA interactions. Recently, the universal protein binding microarray (PBM) was introduced to determine if a DNA motif interacts with proteins in a genome-wide manner.</p>
</sec>
<sec><title>Results</title>
<p>We facilitated the PBM technology using a DsRed fluorescent protein and a concatenated sequence of oligonucleotides. The PBM was designed in such a way that target probes were synthesized as quadruples of all possible 9-mer combinations, permitting unequivocal interpretation of the <italic>cis</italic>
-acting elements. The complimentary DNA strands of the features were synthesized with a primer and DNA polymerase on microarray slides. Proteins were labeled via N-terminal fusion with DsRed fluorescent protein, which circumvents the need for a multi-step incubation. The PBM presented herein confirmed the well-known DNA binding sequences of Cbf1 and CBF1/DREB1B, and it was also applied to elucidate the unidentified <italic>cis</italic>
-acting element of the OsNAC6 rice transcription factor.</p>
</sec>
<sec><title>Conclusion</title>
<p>Our method demonstrated PBM can be conveniently performed by adopting: (1) quadruple 9-mers may increase protein-DNA binding interactions in the microarray, and (2) a one-step incubation shortens the wash and hybridization steps. This technology will facilitate greater understanding of genome-wide interactions between proteins and DNA.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein.</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>GreenGene Biotech Inc, Myongji University, Yongin 449-728, Korea. kim750a11@gmail.com</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19761621</idno>
<idno type="pmid">19761621</idno>
<idno type="doi">10.1186/1471-2199-10-91</idno>
<idno type="wicri:Area/PubMed/Corpus">001F92</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F92</idno>
<idno type="wicri:Area/PubMed/Curation">001F92</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F92</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001E84</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E84</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Quadruple 9-mer-based protein binding microarray with DsRed fusion protein.</title>
<author><name sortKey="Kim, Min Jeong" sort="Kim, Min Jeong" uniqKey="Kim M" first="Min-Jeong" last="Kim">Min-Jeong Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>GreenGene Biotech Inc, Myongji University, Yongin 449-728, Korea. kim750a11@gmail.com</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>GreenGene Biotech Inc, Myongji University, Yongin 449-728</wicri:regionArea>
<wicri:noRegion>Yongin 449-728</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Tae Ho" sort="Lee, Tae Ho" uniqKey="Lee T" first="Tae-Ho" last="Lee">Tae-Ho Lee</name>
</author>
<author><name sortKey="Pahk, Yoon Mok" sort="Pahk, Yoon Mok" uniqKey="Pahk Y" first="Yoon-Mok" last="Pahk">Yoon-Mok Pahk</name>
</author>
<author><name sortKey="Kim, Yul Ho" sort="Kim, Yul Ho" uniqKey="Kim Y" first="Yul-Ho" last="Kim">Yul-Ho Kim</name>
</author>
<author><name sortKey="Park, Hyang Mi" sort="Park, Hyang Mi" uniqKey="Park H" first="Hyang-Mi" last="Park">Hyang-Mi Park</name>
</author>
<author><name sortKey="Choi, Yang Do" sort="Choi, Yang Do" uniqKey="Choi Y" first="Yang Do" last="Choi">Yang Do Choi</name>
</author>
<author><name sortKey="Nahm, Baek Hie" sort="Nahm, Baek Hie" uniqKey="Nahm B" first="Baek Hie" last="Nahm">Baek Hie Nahm</name>
</author>
<author><name sortKey="Kim, Yeon Ki" sort="Kim, Yeon Ki" uniqKey="Kim Y" first="Yeon-Ki" last="Kim">Yeon-Ki Kim</name>
</author>
</analytic>
<series><title level="j">BMC molecular biology</title>
<idno type="eISSN">1471-2199</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Binding Sites</term>
<term>Luminescent Proteins (genetics)</term>
<term>Luminescent Proteins (metabolism)</term>
<term>Oligonucleotides (genetics)</term>
<term>Oligonucleotides (metabolism)</term>
<term>Oryza (chemistry)</term>
<term>Oryza (genetics)</term>
<term>Oryza (metabolism)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Protein Array Analysis (methods)</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse par réseau de protéines ()</term>
<term>Liaison aux protéines</term>
<term>Motifs d'acides aminés</term>
<term>Oligonucléotides (génétique)</term>
<term>Oligonucléotides (métabolisme)</term>
<term>Oryza ()</term>
<term>Oryza (génétique)</term>
<term>Oryza (métabolisme)</term>
<term>Protéines luminescentes (génétique)</term>
<term>Protéines luminescentes (métabolisme)</term>
<term>Protéines végétales ()</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Sites de fixation</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luminescent Proteins</term>
<term>Oligonucleotides</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Luminescent Proteins</term>
<term>Oligonucleotides</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oligonucléotides</term>
<term>Oryza</term>
<term>Protéines luminescentes</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Array Analysis</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Oligonucléotides</term>
<term>Oryza</term>
<term>Protéines luminescentes</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Binding Sites</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse par réseau de protéines</term>
<term>Liaison aux protéines</term>
<term>Motifs d'acides aminés</term>
<term>Oryza</term>
<term>Protéines végétales</term>
<term>Sites de fixation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The interaction between a transcription factor and DNA motif (cis-acting element) is an important regulatory step in gene regulation. Comprehensive genome-wide methods have been developed to characterize protein-DNA interactions. Recently, the universal protein binding microarray (PBM) was introduced to determine if a DNA motif interacts with proteins in a genome-wide manner.</div>
</front>
</TEI>
</pubmed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000713 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000713 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:2754467 |texte= Quadruple 9-mer-based protein binding microarray with DsRed fusion protein }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:19761621" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |