Nucleobase orientation and ordering in films of single-stranded DNA on gold.
Identifieur interne : 000391 ( Ncbi/Merge ); précédent : 000390; suivant : 000392Nucleobase orientation and ordering in films of single-stranded DNA on gold.
Auteurs : Dmitri Y. Petrovykh [États-Unis] ; V. Pérez-Dieste ; Aric Opdahl ; Hiromi Kimura-Suda ; J M Sullivan ; Michael J. Tarlov ; F J Himpsel ; Lloyd J. WhitmanSource :
- Journal of the American Chemical Society [ 0002-7863 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Single-Stranded, Gold, Oligodeoxyribonucleotides, Thymine.
- methods : Spectroscopy, Fourier Transform Infrared, Spectrum Analysis.
- Models, Molecular, Nucleic Acid Conformation, X-Rays.
Abstract
We demonstrate how the orientation and ordering of DNA bases in ultrahigh vacuum (UHV) and ambient environments can be determined using complementary spectroscopic methods. Near-edge X-ray absorption fine structure (NEXAFS) with fluorescence detection, X-ray photoelectron (XPS), and Fourier transform infrared (FTIR) spectroscopies are used to quantify the coverage, chemical composition, orientation, and ordering of thymine bases in model self-assembled monolayers of thymine homo-oligonucleotides [oligo(dT)] on gold. We find that, in monolayers of thiol-modified oligo(dT), thymine bases tend to orient parallel to the Au substrate, and this preferential orientation is significantly more pronounced in monolayers of thiolated 5-mers compared to 25-mers. We interpret this preferential orientation as a signature of significant correlations (local ordering) between individual nuleobases, which offers a way to quantify and compare nucleobase interactions in films under both ambient and UHV conditions.
DOI: 10.1021/ja052443e
PubMed: 16390092
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pubmed:16390092Le document en format XML
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<front><div type="abstract" xml:lang="en">We demonstrate how the orientation and ordering of DNA bases in ultrahigh vacuum (UHV) and ambient environments can be determined using complementary spectroscopic methods. Near-edge X-ray absorption fine structure (NEXAFS) with fluorescence detection, X-ray photoelectron (XPS), and Fourier transform infrared (FTIR) spectroscopies are used to quantify the coverage, chemical composition, orientation, and ordering of thymine bases in model self-assembled monolayers of thymine homo-oligonucleotides [oligo(dT)] on gold. We find that, in monolayers of thiol-modified oligo(dT), thymine bases tend to orient parallel to the Au substrate, and this preferential orientation is significantly more pronounced in monolayers of thiolated 5-mers compared to 25-mers. We interpret this preferential orientation as a signature of significant correlations (local ordering) between individual nuleobases, which offers a way to quantify and compare nucleobase interactions in films under both ambient and UHV conditions.</div>
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