The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.
Identifieur interne : 000145 ( Ncbi/Merge ); précédent : 000144; suivant : 000146The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.
Auteurs : Earle Stellwagen [États-Unis] ; Nancy C. StellwagenSource :
- Electrophoresis [ 0173-0835 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, DNA, Single-Stranded.
- chemical : Acetates, Edetic Acid, Sodium Chloride, Solutions, Tromethamine.
- analysis : Plasmids.
- methods : Electrophoresis, Capillary.
Abstract
The free solution mobility of a high-molecular-weight DNA, linear pUC19, and a 20-bp oligomer called dsA5 have been studied as a function of Tris-acetate-EDTA (TAE) buffer concentration, with and without added NaCl. The two DNAs migrate as separate peaks during capillary electrophoresis, because the mobility of linear pUC19 is higher than that of the 20-bp oligomer. In TAE buffers ranging from 10-400 mM in concentration, the migration times and peak areas of the two DNAs are independent of whether they are electrophoresed separately or in mixtures, indicating that DNA-DNA and DNA-buffer interactions are absent in these solutions. The migration times of the two DNAs vary and the peak areas are not additive when the TAE buffer concentration is reduced to 5 mM or below, indicating that DNA-DNA and DNA-buffer interactions are occurring at very low TAE buffer concentrations. The mobilities of linear pUC19 and dsA5 decrease slowly with increasing conductivity or ionic strength when the conductivity is increased by increasing the TAE buffer concentration. When the Tris buffer concentration is held constant and the conductivity is increased by adding various concentrations of NaCl to the solution, the mobilities of linear pUC19 and dsA5 first increase slightly, then become independent of solution conductivity (or ionic strength), and finally decrease when the NaCl concentration is increased above approximately 50 mM. The mobility variations observed in the various TAE and TAE-NaCl solutions are described qualitatively by Manning's theory, although quantitative agreement is not achieved. The free solution mobilities of single-stranded pUC19 and two 20-base oligonucleotides have also been measured. The free solution mobility of single-stranded pUC19 is approximately 15% lower than that of native pUC19, in agreement with other results in the literature. Somewhat surprisingly, the mobilities of the single- and double-stranded 20-mers are equal to each other in TAE buffers with and without added NaCl.
DOI: 10.1002/1522-2683(200206)23:12<1935::AID-ELPS1935>3.0.CO;2-#
PubMed: 12116139
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002501
- to stream PubMed, to step Curation: 002501
- to stream PubMed, to step Checkpoint: 002345
Links to Exploration step
pubmed:12116139Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.</title><author><name sortKey="Stellwagen, Earle" sort="Stellwagen, Earle" uniqKey="Stellwagen E" first="Earle" last="Stellwagen">Earle Stellwagen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Iowa, Iowa City, IA 52242</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Stellwagen, Nancy C" sort="Stellwagen, Nancy C" uniqKey="Stellwagen N" first="Nancy C" last="Stellwagen">Nancy C. Stellwagen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12116139</idno><idno type="pmid">12116139</idno><idno type="doi">10.1002/1522-2683(200206)23:12<1935::AID-ELPS1935>3.0.CO;2-#</idno><idno type="wicri:Area/PubMed/Corpus">002501</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002501</idno><idno type="wicri:Area/PubMed/Curation">002501</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002501</idno><idno type="wicri:Area/PubMed/Checkpoint">002345</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002345</idno><idno type="wicri:Area/Ncbi/Merge">000145</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.</title><author><name sortKey="Stellwagen, Earle" sort="Stellwagen, Earle" uniqKey="Stellwagen E" first="Earle" last="Stellwagen">Earle Stellwagen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Iowa, Iowa City, IA 52242</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Stellwagen, Nancy C" sort="Stellwagen, Nancy C" uniqKey="Stellwagen N" first="Nancy C" last="Stellwagen">Nancy C. Stellwagen</name></author></analytic><series><title level="j">Electrophoresis</title><idno type="ISSN">0173-0835</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetates</term><term>DNA (analysis)</term><term>DNA, Single-Stranded (analysis)</term><term>Edetic Acid</term><term>Electrophoresis, Capillary (methods)</term><term>Plasmids (analysis)</term><term>Sodium Chloride</term><term>Solutions</term><term>Tromethamine</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>ADN simple brin (analyse)</term><term>Acide édétique</term><term>Acétates</term><term>Chlorure de sodium</term><term>Plasmides (analyse)</term><term>Solutions</term><term>Trométhamine</term><term>Électrophorèse capillaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term><term>DNA, Single-Stranded</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Acetates</term><term>Edetic Acid</term><term>Sodium Chloride</term><term>Solutions</term><term>Tromethamine</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term><term>ADN simple brin</term><term>Plasmides</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide édétique</term><term>Acétates</term><term>Chlorure de sodium</term><term>Solutions</term><term>Trométhamine</term><term>Électrophorèse capillaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The free solution mobility of a high-molecular-weight DNA, linear pUC19, and a 20-bp oligomer called dsA5 have been studied as a function of Tris-acetate-EDTA (TAE) buffer concentration, with and without added NaCl. The two DNAs migrate as separate peaks during capillary electrophoresis, because the mobility of linear pUC19 is higher than that of the 20-bp oligomer. In TAE buffers ranging from 10-400 mM in concentration, the migration times and peak areas of the two DNAs are independent of whether they are electrophoresed separately or in mixtures, indicating that DNA-DNA and DNA-buffer interactions are absent in these solutions. The migration times of the two DNAs vary and the peak areas are not additive when the TAE buffer concentration is reduced to 5 mM or below, indicating that DNA-DNA and DNA-buffer interactions are occurring at very low TAE buffer concentrations. The mobilities of linear pUC19 and dsA5 decrease slowly with increasing conductivity or ionic strength when the conductivity is increased by increasing the TAE buffer concentration. When the Tris buffer concentration is held constant and the conductivity is increased by adding various concentrations of NaCl to the solution, the mobilities of linear pUC19 and dsA5 first increase slightly, then become independent of solution conductivity (or ionic strength), and finally decrease when the NaCl concentration is increased above approximately 50 mM. The mobility variations observed in the various TAE and TAE-NaCl solutions are described qualitatively by Manning's theory, although quantitative agreement is not achieved. The free solution mobilities of single-stranded pUC19 and two 20-base oligonucleotides have also been measured. The free solution mobility of single-stranded pUC19 is approximately 15% lower than that of native pUC19, in agreement with other results in the literature. Somewhat surprisingly, the mobilities of the single- and double-stranded 20-mers are equal to each other in TAE buffers with and without added NaCl.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12116139</PMID><DateCompleted><Year>2003</Year><Month>01</Month><Day>14</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0173-0835</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>12</Issue><PubDate><Year>2002</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Electrophoresis</Title><ISOAbbreviation>Electrophoresis</ISOAbbreviation></Journal><ArticleTitle>The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.</ArticleTitle><Pagination><MedlinePgn>1935-41</MedlinePgn></Pagination><Abstract><AbstractText>The free solution mobility of a high-molecular-weight DNA, linear pUC19, and a 20-bp oligomer called dsA5 have been studied as a function of Tris-acetate-EDTA (TAE) buffer concentration, with and without added NaCl. The two DNAs migrate as separate peaks during capillary electrophoresis, because the mobility of linear pUC19 is higher than that of the 20-bp oligomer. In TAE buffers ranging from 10-400 mM in concentration, the migration times and peak areas of the two DNAs are independent of whether they are electrophoresed separately or in mixtures, indicating that DNA-DNA and DNA-buffer interactions are absent in these solutions. The migration times of the two DNAs vary and the peak areas are not additive when the TAE buffer concentration is reduced to 5 mM or below, indicating that DNA-DNA and DNA-buffer interactions are occurring at very low TAE buffer concentrations. The mobilities of linear pUC19 and dsA5 decrease slowly with increasing conductivity or ionic strength when the conductivity is increased by increasing the TAE buffer concentration. When the Tris buffer concentration is held constant and the conductivity is increased by adding various concentrations of NaCl to the solution, the mobilities of linear pUC19 and dsA5 first increase slightly, then become independent of solution conductivity (or ionic strength), and finally decrease when the NaCl concentration is increased above approximately 50 mM. The mobility variations observed in the various TAE and TAE-NaCl solutions are described qualitatively by Manning's theory, although quantitative agreement is not achieved. The free solution mobilities of single-stranded pUC19 and two 20-base oligonucleotides have also been measured. The free solution mobility of single-stranded pUC19 is approximately 15% lower than that of native pUC19, in agreement with other results in the literature. Somewhat surprisingly, the mobilities of the single- and double-stranded 20-mers are equal to each other in TAE buffers with and without added NaCl.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stellwagen</LastName><ForeName>Earle</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stellwagen</LastName><ForeName>Nancy C</ForeName><Initials>NC</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>GM29690</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>GM61009</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Electrophoresis</MedlineTA><NlmUniqueID>8204476</NlmUniqueID><ISSNLinking>0173-0835</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000085">Acetates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004277">DNA, Single-Stranded</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012996">Solutions</NameOfSubstance></Chemical><Chemical><RegistryNumber>023C2WHX2V</RegistryNumber><NameOfSubstance UI="D014325">Tromethamine</NameOfSubstance></Chemical><Chemical><RegistryNumber>451W47IQ8X</RegistryNumber><NameOfSubstance UI="D012965">Sodium Chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>9G34HU7RV0</RegistryNumber><NameOfSubstance UI="D004492">Edetic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000085" MajorTopicYN="Y">Acetates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004277" MajorTopicYN="N">DNA, Single-Stranded</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004492" MajorTopicYN="Y">Edetic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019075" MajorTopicYN="N">Electrophoresis, Capillary</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012965" MajorTopicYN="Y">Sodium Chloride</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012996" MajorTopicYN="N">Solutions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014325" MajorTopicYN="Y">Tromethamine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>7</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>1</Month><Day>15</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>7</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12116139</ArticleId><ArticleId IdType="doi">10.1002/1522-2683(200206)23:12<1935::AID-ELPS1935>3.0.CO;2-#</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Iowa City</li></settlement><orgName><li>Université de l'Iowa</li></orgName></list><tree><noCountry><name sortKey="Stellwagen, Nancy C" sort="Stellwagen, Nancy C" uniqKey="Stellwagen N" first="Nancy C" last="Stellwagen">Nancy C. Stellwagen</name></noCountry><country name="États-Unis"><region name="Iowa"><name sortKey="Stellwagen, Earle" sort="Stellwagen, Earle" uniqKey="Stellwagen E" first="Earle" last="Stellwagen">Earle Stellwagen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000145 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000145 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:12116139
|texte= The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:12116139" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |