Activation of nonspecific cytotoxic cells (NCC) with synthetic oligodeoxynucleotides and bacterial genomic DNA: binding, specificity and identification of unique immunostimulatory motifs.
Identifieur interne : 000120 ( Ncbi/Merge ); précédent : 000119; suivant : 000121Activation of nonspecific cytotoxic cells (NCC) with synthetic oligodeoxynucleotides and bacterial genomic DNA: binding, specificity and identification of unique immunostimulatory motifs.
Auteurs : M. Oumouna [États-Unis] ; L. Jaso-Friedmann ; D L EvansSource :
- Developmental and comparative immunology [ 0145-305X ] ; 2002.
Descripteurs français
- KwdFr :
- ADN bactérien (), ADN bactérien (pharmacologie), Animaux, Cytosine (métabolisme), Cytotoxicité immunologique (), Femelle, Humains, Immunité innée (), Lipopolysaccharides (pharmacologie), Mâle, Méthylation de l'ADN, Oligodésoxyribonucléotides (), Oligodésoxyribonucléotides (métabolisme), Oligodésoxyribonucléotides (pharmacologie), Poissons-chats (immunologie), Sensibilité et spécificité, Souris.
- MESH :
- immunologie : Poissons-chats.
- métabolisme : Cytosine, Oligodésoxyribonucléotides.
- pharmacologie : ADN bactérien, Lipopolysaccharides, Oligodésoxyribonucléotides.
- ADN bactérien, Animaux, Cytotoxicité immunologique, Femelle, Humains, Immunité innée, Mâle, Méthylation de l'ADN, Oligodésoxyribonucléotides, Sensibilité et spécificité, Souris.
English descriptors
- KwdEn :
- Animals, Catfishes (immunology), Cytosine (metabolism), Cytotoxicity, Immunologic (drug effects), DNA Methylation, DNA, Bacterial (chemistry), DNA, Bacterial (pharmacology), Female, Humans, Immunity, Innate (drug effects), Lipopolysaccharides (pharmacology), Male, Mice, Oligodeoxyribonucleotides (chemistry), Oligodeoxyribonucleotides (metabolism), Oligodeoxyribonucleotides (pharmacology), Sensitivity and Specificity.
- MESH :
- chemical , chemistry : DNA, Bacterial, Oligodeoxyribonucleotides.
- chemical , metabolism : Cytosine, Oligodeoxyribonucleotides.
- drug effects : Cytotoxicity, Immunologic, Immunity, Innate.
- immunology : Catfishes.
- chemical , pharmacology : DNA, Bacterial, Lipopolysaccharides, Oligodeoxyribonucleotides.
- Animals, DNA Methylation, Female, Humans, Male, Mice, Sensitivity and Specificity.
Abstract
We have analyzed the effects of synthetic oligodeoxynucleotides (sODNs) and bacterial DNA (bDNA) on the in vitro activation of NCC. Teleost NCC recognition of DNA appeared to differ from that which occurs in higher vertebrates. NCC contain at least two different receptor specificities for DNA. Both oligodeoxyguanosine 20-mers (dG20) and 5'-TGCTGCTTGTGCTTGTGCTT-3' (4GC-2T) bound specifically to NCC. The existence of different receptor specificities was indicated by reciprocal cold target inhibition experiments. dG20 competed with 4GC-2T binding but sODNs composed of GpC or CpG nests did not compete with recognition by NCC of the dG20. ODN binding by NCC primarily depended on the presence of GpC or CpG nests with a preference for -G- serving as the anchor nucleotide. Secondarily, and similar to models of ODN activation in mammals, palindrome sequences of pu-pu-CpG-py-py activated NCC cytotoxicity. Additional analysis of the requirements for ODN activation indicated that guanosine could not substitute for adenosine as a purine spacer and that CpG motifs containing flanking thymidine (i.e.-GTCpGTT-) augmented the activity of the sODN containing this flanking base. Other evidence for the participation of both G and C in the recognition of specific nucleotides by NCC was that poly-dC20, dA20 or dT20 had no activating properties. Methylation of all cytosine nucleotides within an ODN abrogated activation. A canonical ODN motif of 5'-C/AT/AGCTT-3' can now be suggested for teleosts. Additional studies were done to examine the effects of in vitro treatment of NCC with bDNA. bDNA from three different disease isolates of Streptococcus iniae activated NCC cytotoxicity. Treatment of the bDNA with DNase abrogated the enhancement of cytotoxicity. Also, treatment of NCC with eukaryotic DNA had no effects on cytotoxicity. These studies suggested that NCC recognize bacterial nonmethylated DNA. The consequences of these interactions may be increased innate and acquired anti-bacterial immunity.
DOI: 10.1016/s0145-305x(01)00068-4
PubMed: 11755675
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Oumouna</name><affiliation wicri:level="2"><nlm:affiliation>Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Jaso Friedmann, L" sort="Jaso Friedmann, L" uniqKey="Jaso Friedmann L" first="L" last="Jaso-Friedmann">L. Jaso-Friedmann</name></author><author><name sortKey="Evans, D L" sort="Evans, D L" uniqKey="Evans D" first="D L" last="Evans">D L Evans</name></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="ISSN">0145-305X</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Catfishes (immunology)</term><term>Cytosine (metabolism)</term><term>Cytotoxicity, Immunologic (drug effects)</term><term>DNA Methylation</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (pharmacology)</term><term>Female</term><term>Humans</term><term>Immunity, Innate (drug effects)</term><term>Lipopolysaccharides (pharmacology)</term><term>Male</term><term>Mice</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Oligodeoxyribonucleotides (metabolism)</term><term>Oligodeoxyribonucleotides (pharmacology)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien ()</term><term>ADN bactérien (pharmacologie)</term><term>Animaux</term><term>Cytosine (métabolisme)</term><term>Cytotoxicité immunologique ()</term><term>Femelle</term><term>Humains</term><term>Immunité innée ()</term><term>Lipopolysaccharides (pharmacologie)</term><term>Mâle</term><term>Méthylation de l'ADN</term><term>Oligodésoxyribonucléotides ()</term><term>Oligodésoxyribonucléotides (métabolisme)</term><term>Oligodésoxyribonucléotides (pharmacologie)</term><term>Poissons-chats (immunologie)</term><term>Sensibilité et spécificité</term><term>Souris</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytosine</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cytotoxicity, Immunologic</term><term>Immunity, Innate</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Poissons-chats</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Catfishes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytosine</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>ADN bactérien</term><term>Lipopolysaccharides</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>DNA, Bacterial</term><term>Lipopolysaccharides</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>DNA Methylation</term><term>Female</term><term>Humans</term><term>Male</term><term>Mice</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN bactérien</term><term>Animaux</term><term>Cytotoxicité immunologique</term><term>Femelle</term><term>Humains</term><term>Immunité innée</term><term>Mâle</term><term>Méthylation de l'ADN</term><term>Oligodésoxyribonucléotides</term><term>Sensibilité et spécificité</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have analyzed the effects of synthetic oligodeoxynucleotides (sODNs) and bacterial DNA (bDNA) on the in vitro activation of NCC. Teleost NCC recognition of DNA appeared to differ from that which occurs in higher vertebrates. NCC contain at least two different receptor specificities for DNA. Both oligodeoxyguanosine 20-mers (dG20) and 5'-TGCTGCTTGTGCTTGTGCTT-3' (4GC-2T) bound specifically to NCC. The existence of different receptor specificities was indicated by reciprocal cold target inhibition experiments. dG20 competed with 4GC-2T binding but sODNs composed of GpC or CpG nests did not compete with recognition by NCC of the dG20. ODN binding by NCC primarily depended on the presence of GpC or CpG nests with a preference for -G- serving as the anchor nucleotide. Secondarily, and similar to models of ODN activation in mammals, palindrome sequences of pu-pu-CpG-py-py activated NCC cytotoxicity. Additional analysis of the requirements for ODN activation indicated that guanosine could not substitute for adenosine as a purine spacer and that CpG motifs containing flanking thymidine (i.e.-GTCpGTT-) augmented the activity of the sODN containing this flanking base. Other evidence for the participation of both G and C in the recognition of specific nucleotides by NCC was that poly-dC20, dA20 or dT20 had no activating properties. Methylation of all cytosine nucleotides within an ODN abrogated activation. A canonical ODN motif of 5'-C/AT/AGCTT-3' can now be suggested for teleosts. Additional studies were done to examine the effects of in vitro treatment of NCC with bDNA. bDNA from three different disease isolates of Streptococcus iniae activated NCC cytotoxicity. Treatment of the bDNA with DNase abrogated the enhancement of cytotoxicity. Also, treatment of NCC with eukaryotic DNA had no effects on cytotoxicity. These studies suggested that NCC recognize bacterial nonmethylated DNA. The consequences of these interactions may be increased innate and acquired anti-bacterial immunity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11755675</PMID><DateCompleted><Year>2002</Year><Month>03</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0145-305X</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>3</Issue><PubDate><Year>2002</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Developmental and comparative immunology</Title><ISOAbbreviation>Dev. Comp. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Activation of nonspecific cytotoxic cells (NCC) with synthetic oligodeoxynucleotides and bacterial genomic DNA: binding, specificity and identification of unique immunostimulatory motifs.</ArticleTitle><Pagination><MedlinePgn>257-69</MedlinePgn></Pagination><Abstract><AbstractText>We have analyzed the effects of synthetic oligodeoxynucleotides (sODNs) and bacterial DNA (bDNA) on the in vitro activation of NCC. Teleost NCC recognition of DNA appeared to differ from that which occurs in higher vertebrates. NCC contain at least two different receptor specificities for DNA. Both oligodeoxyguanosine 20-mers (dG20) and 5'-TGCTGCTTGTGCTTGTGCTT-3' (4GC-2T) bound specifically to NCC. The existence of different receptor specificities was indicated by reciprocal cold target inhibition experiments. dG20 competed with 4GC-2T binding but sODNs composed of GpC or CpG nests did not compete with recognition by NCC of the dG20. ODN binding by NCC primarily depended on the presence of GpC or CpG nests with a preference for -G- serving as the anchor nucleotide. Secondarily, and similar to models of ODN activation in mammals, palindrome sequences of pu-pu-CpG-py-py activated NCC cytotoxicity. Additional analysis of the requirements for ODN activation indicated that guanosine could not substitute for adenosine as a purine spacer and that CpG motifs containing flanking thymidine (i.e.-GTCpGTT-) augmented the activity of the sODN containing this flanking base. Other evidence for the participation of both G and C in the recognition of specific nucleotides by NCC was that poly-dC20, dA20 or dT20 had no activating properties. Methylation of all cytosine nucleotides within an ODN abrogated activation. A canonical ODN motif of 5'-C/AT/AGCTT-3' can now be suggested for teleosts. Additional studies were done to examine the effects of in vitro treatment of NCC with bDNA. bDNA from three different disease isolates of Streptococcus iniae activated NCC cytotoxicity. Treatment of the bDNA with DNase abrogated the enhancement of cytotoxicity. Also, treatment of NCC with eukaryotic DNA had no effects on cytotoxicity. These studies suggested that NCC recognize bacterial nonmethylated DNA. The consequences of these interactions may be increased innate and acquired anti-bacterial immunity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oumouna</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaso-Friedmann</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Evans</LastName><ForeName>D L</ForeName><Initials>DL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Dev Comp Immunol</MedlineTA><NlmUniqueID>7708205</NlmUniqueID><ISSNLinking>0145-305X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008070">Lipopolysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>8J337D1HZY</RegistryNumber><NameOfSubstance UI="D003596">Cytosine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002397" MajorTopicYN="N">Catfishes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003596" MajorTopicYN="N">Cytosine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003602" MajorTopicYN="N">Cytotoxicity, Immunologic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019175" MajorTopicYN="N">DNA Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008070" MajorTopicYN="N">Lipopolysaccharides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>3</Month><Day>22</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11755675</ArticleId><ArticleId IdType="pii">S0145305X01000684</ArticleId><ArticleId IdType="doi">10.1016/s0145-305x(01)00068-4</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Evans, D L" sort="Evans, D L" uniqKey="Evans D" first="D L" last="Evans">D L Evans</name><name sortKey="Jaso Friedmann, L" sort="Jaso Friedmann, L" uniqKey="Jaso Friedmann L" first="L" last="Jaso-Friedmann">L. Jaso-Friedmann</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Oumouna, M" sort="Oumouna, M" uniqKey="Oumouna M" first="M" last="Oumouna">M. Oumouna</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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