Antigenic domains of the open reading frame 2-encoded protein of hepatitis E virus.
Identifieur interne : 000028 ( Ncbi/Merge ); précédent : 000027; suivant : 000029Antigenic domains of the open reading frame 2-encoded protein of hepatitis E virus.
Auteurs : Y E Khudyakov [États-Unis] ; E N Lopareva ; D L Jue ; T K Crews ; S P Thyagarajan ; H A FieldsSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 1999.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Antigènes d'hépatite virale.
- immunologie : Anticorps de l'hépatite, Antigènes d'hépatite virale, Immunoglobuline G, Immunoglobuline M, Virus de l'hépatite E.
- Cadres ouverts de lecture, Humains, Épitopes.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Hepatitis Antigens.
- chemical , immunology : Hepatitis Antibodies, Hepatitis Antigens, Immunoglobulin G, Immunoglobulin M.
- chemical : Epitopes.
- immunology : Hepatitis E virus.
- Humans, Open Reading Frames.
Abstract
The antigenic composition of the hepatitis E virus (HEV) protein encoded by open reading frame 2 (ORF2) was determined by using synthetic peptides. Three sets of overlapping 18-, 25-, and 30-mer peptides, with each set spanning the entire ORF2 protein of the HEV Burma strain, were synthesized. All synthetic peptides were tested by enzyme immunoassay against a panel of 32 anti-HEV-positive serum specimens obtained from acutely HEV-infected persons. Six antigenic domains within the ORF2 protein were identified. Domains 1 and 6 located at the N and C termini of the ORF2 protein, respectively, contain strong immunoglobulin G (IgG) and IgM antigenic epitopes that can be efficiently modeled with peptides of different sizes. In contrast, antigenic epitopes identified within the two central domains (3 and 4) were modeled more efficiently with 30-mer peptides than with either 18- or 25-mers. Domain 2 located at amino acids (aa) 143 to 222 was modeled best with 25-mer peptides. A few 30-mer synthetic peptides derived from domain 5 identified at aa 490 to 579 demonstrated strong IgM antigenic reactivity. Several 30-mer synthetic peptides derived from domains 1, 4, and 6 immunoreacted with IgG or IgM with more than 70% of anti-HEV-positive serum specimens. Thus, the results of this study demonstrate the existence of six diagnostically relevant antigenic domains within the HEV ORF2 protein.
PubMed: 10449466
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S. Department of Health and Human Services, Atlanta, Georgia 30333, USA. yek0@cdc.gov</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Hepatitis Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U. S. Department of Health and Human Services, Atlanta, Georgia 30333</wicri:regionArea><wicri:noRegion>Georgia 30333</wicri:noRegion></affiliation></author><author><name sortKey="Lopareva, E N" sort="Lopareva, E N" uniqKey="Lopareva E" first="E N" last="Lopareva">E N Lopareva</name></author><author><name sortKey="Jue, D L" sort="Jue, D L" uniqKey="Jue D" first="D L" last="Jue">D L Jue</name></author><author><name sortKey="Crews, T K" sort="Crews, T K" uniqKey="Crews T" first="T K" last="Crews">T K Crews</name></author><author><name sortKey="Thyagarajan, S P" sort="Thyagarajan, S P" uniqKey="Thyagarajan S" first="S P" last="Thyagarajan">S P Thyagarajan</name></author><author><name sortKey="Fields, H A" sort="Fields, H A" uniqKey="Fields H" first="H A" last="Fields">H A Fields</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Epitopes</term><term>Hepatitis Antibodies (immunology)</term><term>Hepatitis Antigens (genetics)</term><term>Hepatitis Antigens (immunology)</term><term>Hepatitis E virus (immunology)</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Immunoglobulin M (immunology)</term><term>Open Reading Frames</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps de l'hépatite (immunologie)</term><term>Antigènes d'hépatite virale (génétique)</term><term>Antigènes d'hépatite virale (immunologie)</term><term>Cadres ouverts de lecture</term><term>Humains</term><term>Immunoglobuline G (immunologie)</term><term>Immunoglobuline M (immunologie)</term><term>Virus de l'hépatite E (immunologie)</term><term>Épitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hepatitis Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Hepatitis Antibodies</term><term>Hepatitis Antigens</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Epitopes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'hépatite virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps de l'hépatite</term><term>Antigènes d'hépatite virale</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term><term>Virus de l'hépatite E</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hepatitis E virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Open Reading Frames</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Humains</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The antigenic composition of the hepatitis E virus (HEV) protein encoded by open reading frame 2 (ORF2) was determined by using synthetic peptides. Three sets of overlapping 18-, 25-, and 30-mer peptides, with each set spanning the entire ORF2 protein of the HEV Burma strain, were synthesized. All synthetic peptides were tested by enzyme immunoassay against a panel of 32 anti-HEV-positive serum specimens obtained from acutely HEV-infected persons. Six antigenic domains within the ORF2 protein were identified. Domains 1 and 6 located at the N and C termini of the ORF2 protein, respectively, contain strong immunoglobulin G (IgG) and IgM antigenic epitopes that can be efficiently modeled with peptides of different sizes. In contrast, antigenic epitopes identified within the two central domains (3 and 4) were modeled more efficiently with 30-mer peptides than with either 18- or 25-mers. Domain 2 located at amino acids (aa) 143 to 222 was modeled best with 25-mer peptides. A few 30-mer synthetic peptides derived from domain 5 identified at aa 490 to 579 demonstrated strong IgM antigenic reactivity. Several 30-mer synthetic peptides derived from domains 1, 4, and 6 immunoreacted with IgG or IgM with more than 70% of anti-HEV-positive serum specimens. Thus, the results of this study demonstrate the existence of six diagnostically relevant antigenic domains within the HEV ORF2 protein.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10449466</PMID><DateCompleted><Year>1999</Year><Month>09</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>9</Issue><PubDate><Year>1999</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Antigenic domains of the open reading frame 2-encoded protein of hepatitis E virus.</ArticleTitle><Pagination><MedlinePgn>2863-71</MedlinePgn></Pagination><Abstract><AbstractText>The antigenic composition of the hepatitis E virus (HEV) protein encoded by open reading frame 2 (ORF2) was determined by using synthetic peptides. Three sets of overlapping 18-, 25-, and 30-mer peptides, with each set spanning the entire ORF2 protein of the HEV Burma strain, were synthesized. All synthetic peptides were tested by enzyme immunoassay against a panel of 32 anti-HEV-positive serum specimens obtained from acutely HEV-infected persons. Six antigenic domains within the ORF2 protein were identified. Domains 1 and 6 located at the N and C termini of the ORF2 protein, respectively, contain strong immunoglobulin G (IgG) and IgM antigenic epitopes that can be efficiently modeled with peptides of different sizes. In contrast, antigenic epitopes identified within the two central domains (3 and 4) were modeled more efficiently with 30-mer peptides than with either 18- or 25-mers. Domain 2 located at amino acids (aa) 143 to 222 was modeled best with 25-mer peptides. A few 30-mer synthetic peptides derived from domain 5 identified at aa 490 to 579 demonstrated strong IgM antigenic reactivity. Several 30-mer synthetic peptides derived from domains 1, 4, and 6 immunoreacted with IgG or IgM with more than 70% of anti-HEV-positive serum specimens. Thus, the results of this study demonstrate the existence of six diagnostically relevant antigenic domains within the HEV ORF2 protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Khudyakov</LastName><ForeName>Y E</ForeName><Initials>YE</Initials><AffiliationInfo><Affiliation>Hepatitis Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U. S. 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