In vitro evaluation of the haemostatic value of the LFB-von Willebrand factor concentrate.
Identifieur interne : 000002 ( Ncbi/Merge ); précédent : 000001; suivant : 000003In vitro evaluation of the haemostatic value of the LFB-von Willebrand factor concentrate.
Auteurs : C. Mazurier [France]Source :
- Haemophilia : the official journal of the World Federation of Hemophilia [ 1351-8216 ] ; 1998.
Descripteurs français
- KwdFr :
- Collagène (métabolisme), Complexe glycoprotéique GPIb-IX plaquettaire, Complexe glycoprotéique IIb-IIIa de la membrane plaquettaire (métabolisme), Facteur VIII (métabolisme), Facteur de von Willebrand (analyse), Facteur de von Willebrand (métabolisme), Glycoprotéines de membrane plaquettaire, Humains, Hémostatiques (analyse), Hémostatiques (métabolisme), Récepteurs de surface cellulaire (métabolisme).
- MESH :
- analyse : Facteur de von Willebrand, Hémostatiques.
- métabolisme : Collagène, Complexe glycoprotéique IIb-IIIa de la membrane plaquettaire, Facteur VIII, Facteur de von Willebrand, Hémostatiques, Récepteurs de surface cellulaire.
- Complexe glycoprotéique GPIb-IX plaquettaire, Glycoprotéines de membrane plaquettaire, Humains.
English descriptors
- KwdEn :
- Collagen (metabolism), Factor VIII (metabolism), Hemostatics (analysis), Hemostatics (metabolism), Humans, Platelet Glycoprotein GPIIb-IIIa Complex (metabolism), Platelet Glycoprotein GPIb-IX Complex, Platelet Membrane Glycoproteins, Receptors, Cell Surface (metabolism), von Willebrand Factor (analysis), von Willebrand Factor (metabolism).
- MESH :
- chemical , analysis : Hemostatics, von Willebrand Factor.
- chemical , metabolism : Collagen, Factor VIII, Hemostatics, Platelet Glycoprotein GPIIb-IIIa Complex, Receptors, Cell Surface, von Willebrand Factor.
- Humans, Platelet Glycoprotein GPIb-IX Complex, Platelet Membrane Glycoproteins.
Abstract
The structural and functional studies of the concentrate of von Willebrand factor (vWF) manufactured by LFB have been first performed as part of the preclinical development of this product specially intended for the treatment of von Willebrand disease. The electrophoretic analyses showed the high purity of LFB-vWF concentrate (specific activity of 100 IU of ristocetin cofactor activity per milligram of protein) and the multimeric pattern attesting the presence of high molecular weight multimeters (> or = 15-mers). The measurements of vWF capacity to bind to: (1) platelet glycoprotein (GP) Ib in the presence of ristocetin; (2) GPIIb/IIIa in the presence of thrombin; (3) types I and III collagen; (4) factor VIII (FVIII) gave evidence that the functional activity of purified vWF was similar to that of native plasma vWF. Furthermore the LFB-vWF concentrate was able to promote platelet adhesion to collagen. The release of the therapeutic batches of this product rely presently on their potency measured with the ristocetin cofactor activity (40-70 IU ml-1) and the quantitative evaluation of vWF multimeric distribution studied using electrophoresis in 1.5% agarose. These validated techniques ensure the consistency and haemostatic properties of the different lots of LFB-vWF concentrate.
DOI: 10.1046/j.1365-2516.1998.0040s3040.x
PubMed: 10028317
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pubmed:10028317Le document en format XML
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<country xml:lang="fr">France</country>
<wicri:regionArea>Unité de Développement Pré-Clinique, Laboratoire Français du Fractionnement et des Biotechnologies, Lille</wicri:regionArea>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Collagen (metabolism)</term>
<term>Factor VIII (metabolism)</term>
<term>Hemostatics (analysis)</term>
<term>Hemostatics (metabolism)</term>
<term>Humans</term>
<term>Platelet Glycoprotein GPIIb-IIIa Complex (metabolism)</term>
<term>Platelet Glycoprotein GPIb-IX Complex</term>
<term>Platelet Membrane Glycoproteins</term>
<term>Receptors, Cell Surface (metabolism)</term>
<term>von Willebrand Factor (analysis)</term>
<term>von Willebrand Factor (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Collagène (métabolisme)</term>
<term>Complexe glycoprotéique GPIb-IX plaquettaire</term>
<term>Complexe glycoprotéique IIb-IIIa de la membrane plaquettaire (métabolisme)</term>
<term>Facteur VIII (métabolisme)</term>
<term>Facteur de von Willebrand (analyse)</term>
<term>Facteur de von Willebrand (métabolisme)</term>
<term>Glycoprotéines de membrane plaquettaire</term>
<term>Humains</term>
<term>Hémostatiques (analyse)</term>
<term>Hémostatiques (métabolisme)</term>
<term>Récepteurs de surface cellulaire (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemostatics</term>
<term>von Willebrand Factor</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Collagen</term>
<term>Factor VIII</term>
<term>Hemostatics</term>
<term>Platelet Glycoprotein GPIIb-IIIa Complex</term>
<term>Receptors, Cell Surface</term>
<term>von Willebrand Factor</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Facteur de von Willebrand</term>
<term>Hémostatiques</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Collagène</term>
<term>Complexe glycoprotéique IIb-IIIa de la membrane plaquettaire</term>
<term>Facteur VIII</term>
<term>Facteur de von Willebrand</term>
<term>Hémostatiques</term>
<term>Récepteurs de surface cellulaire</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Platelet Glycoprotein GPIb-IX Complex</term>
<term>Platelet Membrane Glycoproteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Complexe glycoprotéique GPIb-IX plaquettaire</term>
<term>Glycoprotéines de membrane plaquettaire</term>
<term>Humains</term>
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<front><div type="abstract" xml:lang="en">The structural and functional studies of the concentrate of von Willebrand factor (vWF) manufactured by LFB have been first performed as part of the preclinical development of this product specially intended for the treatment of von Willebrand disease. The electrophoretic analyses showed the high purity of LFB-vWF concentrate (specific activity of 100 IU of ristocetin cofactor activity per milligram of protein) and the multimeric pattern attesting the presence of high molecular weight multimeters (> or = 15-mers). The measurements of vWF capacity to bind to: (1) platelet glycoprotein (GP) Ib in the presence of ristocetin; (2) GPIIb/IIIa in the presence of thrombin; (3) types I and III collagen; (4) factor VIII (FVIII) gave evidence that the functional activity of purified vWF was similar to that of native plasma vWF. Furthermore the LFB-vWF concentrate was able to promote platelet adhesion to collagen. The release of the therapeutic batches of this product rely presently on their potency measured with the ristocetin cofactor activity (40-70 IU ml-1) and the quantitative evaluation of vWF multimeric distribution studied using electrophoresis in 1.5% agarose. These validated techniques ensure the consistency and haemostatic properties of the different lots of LFB-vWF concentrate.</div>
</front>
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<Title>Haemophilia : the official journal of the World Federation of Hemophilia</Title>
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<Abstract><AbstractText>The structural and functional studies of the concentrate of von Willebrand factor (vWF) manufactured by LFB have been first performed as part of the preclinical development of this product specially intended for the treatment of von Willebrand disease. The electrophoretic analyses showed the high purity of LFB-vWF concentrate (specific activity of 100 IU of ristocetin cofactor activity per milligram of protein) and the multimeric pattern attesting the presence of high molecular weight multimeters (> or = 15-mers). The measurements of vWF capacity to bind to: (1) platelet glycoprotein (GP) Ib in the presence of ristocetin; (2) GPIIb/IIIa in the presence of thrombin; (3) types I and III collagen; (4) factor VIII (FVIII) gave evidence that the functional activity of purified vWF was similar to that of native plasma vWF. Furthermore the LFB-vWF concentrate was able to promote platelet adhesion to collagen. The release of the therapeutic batches of this product rely presently on their potency measured with the ristocetin cofactor activity (40-70 IU ml-1) and the quantitative evaluation of vWF multimeric distribution studied using electrophoresis in 1.5% agarose. These validated techniques ensure the consistency and haemostatic properties of the different lots of LFB-vWF concentrate.</AbstractText>
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