Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies
Identifieur interne : 002190 ( Ncbi/Curation ); précédent : 002189; suivant : 002191Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies
Auteurs : Chia Chiu Lim [Malaisie] ; Patrick C. Y. Woo [République populaire de Chine] ; Theam Soon Lim [Malaisie]Source :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
Antibody phage display has been pivotal in the quest to generate human monoclonal antibodies for biomedical and research applications. Target antigen preparation is a main bottleneck associated with the panning process. This includes production complexity, downstream purification, quality and yield. In many instances, purified antigens are preferred for panning but this may not be possible for certain difficult target antigens. Here, we describe an improved procedure of affinity selection against crude or non-purified antigen by saturation of non-binders with blocking agents to promote positive binder enrichment termed as
Url:
DOI: 10.1038/s41598-019-42628-6
PubMed: 30988390
PubMed Central: 6465254
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000458
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000458
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000343
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000567
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000567
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000604
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002190
Links to Exploration step
PMC:6465254Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies</title>
<author><name sortKey="Lim, Chia Chiu" sort="Lim, Chia Chiu" uniqKey="Lim C" first="Chia Chiu" last="Lim">Chia Chiu Lim</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Institute for Research in Molecular Medicine,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000121742757</institution-id>
<institution-id institution-id-type="GRID">grid.194645.b</institution-id>
<institution>Department of Microbiology,</institution>
<institution>The University of Hong Kong,</institution>
</institution-wrap>
Hong Kong SAR, People’s Republic of China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Hong Kong SAR</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lim, Theam Soon" sort="Lim, Theam Soon" uniqKey="Lim T" first="Theam Soon" last="Lim">Theam Soon Lim</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Institute for Research in Molecular Medicine,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Analytical Biochemistry Research Centre,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">30988390</idno>
<idno type="pmc">6465254</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465254</idno>
<idno type="RBID">PMC:6465254</idno>
<idno type="doi">10.1038/s41598-019-42628-6</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000458</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000458</idno>
<idno type="wicri:Area/Pmc/Curation">000458</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000458</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000343</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000343</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:30988390</idno>
<idno type="wicri:Area/PubMed/Corpus">000567</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000567</idno>
<idno type="wicri:Area/PubMed/Curation">000567</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000567</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000604</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000604</idno>
<idno type="wicri:Area/Ncbi/Merge">002190</idno>
<idno type="wicri:Area/Ncbi/Curation">002190</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies</title>
<author><name sortKey="Lim, Chia Chiu" sort="Lim, Chia Chiu" uniqKey="Lim C" first="Chia Chiu" last="Lim">Chia Chiu Lim</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Institute for Research in Molecular Medicine,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000121742757</institution-id>
<institution-id institution-id-type="GRID">grid.194645.b</institution-id>
<institution>Department of Microbiology,</institution>
<institution>The University of Hong Kong,</institution>
</institution-wrap>
Hong Kong SAR, People’s Republic of China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Hong Kong SAR</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lim, Theam Soon" sort="Lim, Theam Soon" uniqKey="Lim T" first="Theam Soon" last="Lim">Theam Soon Lim</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Institute for Research in Molecular Medicine,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2294 3534</institution-id>
<institution-id institution-id-type="GRID">grid.11875.3a</institution-id>
<institution>Analytical Biochemistry Research Centre,</institution>
<institution>Universiti Sains Malaysia,</institution>
</institution-wrap>
11800 Penang, Malaysia</nlm:aff>
<country xml:lang="fr">Malaisie</country>
<wicri:regionArea>11800 Penang</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">Antibody phage display has been pivotal in the quest to generate human monoclonal antibodies for biomedical and research applications. Target antigen preparation is a main bottleneck associated with the panning process. This includes production complexity, downstream purification, quality and yield. In many instances, purified antigens are preferred for panning but this may not be possible for certain difficult target antigens. Here, we describe an improved procedure of affinity selection against crude or non-purified antigen by saturation of non-binders with blocking agents to promote positive binder enrichment termed as <italic>Yin-Yang</italic>
panning. A naïve human scFv library with kappa light chain repertoire with a library size of 10<sup>9</sup>
was developed. The improved <italic>Yin-Yang</italic>
biopanning process was able to enrich monoclonal antibodies specific to the MERS-CoV nucleoprotein. Three unique monoclonal antibodies were isolated in the process. The <italic>Yin-Yang</italic>
biopanning method highlights the possibility of utilizing crude antigens for the isolation of monoclonal antibodies by phage display.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Smith, G" uniqKey="Smith G">G Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frenzel, Andre" uniqKey="Frenzel A">André Frenzel</name>
</author>
<author><name sortKey="Kugler, Jonas" uniqKey="Kugler J">Jonas Kügler</name>
</author>
<author><name sortKey="Helmsing, Saskia" uniqKey="Helmsing S">Saskia Helmsing</name>
</author>
<author><name sortKey="Meier, Doris" uniqKey="Meier D">Doris Meier</name>
</author>
<author><name sortKey="Schirrmann, Thomas" uniqKey="Schirrmann T">Thomas Schirrmann</name>
</author>
<author><name sortKey="Hust, Michael" uniqKey="Hust M">Michael Hust</name>
</author>
<author><name sortKey="Dubel, Stefan" uniqKey="Dubel S">Stefan Dübel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marks, Jd" uniqKey="Marks J">JD Marks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ponsel, D" uniqKey="Ponsel D">D Ponsel</name>
</author>
<author><name sortKey="Neugebauer, J" uniqKey="Neugebauer J">J Neugebauer</name>
</author>
<author><name sortKey="Ladetzki Baehs, K" uniqKey="Ladetzki Baehs K">K Ladetzki-Baehs</name>
</author>
<author><name sortKey="Tissot, K" uniqKey="Tissot K">K Tissot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tohidkia, Mr" uniqKey="Tohidkia M">MR Tohidkia</name>
</author>
<author><name sortKey="Barar, J" uniqKey="Barar J">J Barar</name>
</author>
<author><name sortKey="Asadi, F" uniqKey="Asadi F">F Asadi</name>
</author>
<author><name sortKey="Omidi, Y" uniqKey="Omidi Y">Y Omidi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhao, A" uniqKey="Zhao A">A Zhao</name>
</author>
<author><name sortKey="Tohidkia, Mr" uniqKey="Tohidkia M">MR Tohidkia</name>
</author>
<author><name sortKey="Siegel, Dl" uniqKey="Siegel D">DL Siegel</name>
</author>
<author><name sortKey="Coukos, G" uniqKey="Coukos G">G Coukos</name>
</author>
<author><name sortKey="Omidi, Y" uniqKey="Omidi Y">Y Omidi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bornholdt, Za" uniqKey="Bornholdt Z">ZA Bornholdt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoogenboom, Hr" uniqKey="Hoogenboom H">HR Hoogenboom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhou, H" uniqKey="Zhou H">H Zhou</name>
</author>
<author><name sortKey="Zhang, Y L" uniqKey="Zhang Y">Y-L Zhang</name>
</author>
<author><name sortKey="Lu, G" uniqKey="Lu G">G Lu</name>
</author>
<author><name sortKey="Ji, H" uniqKey="Ji H">H Ji</name>
</author>
<author><name sortKey="Rodi, Cp" uniqKey="Rodi C">CP Rodi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lebani, K" uniqKey="Lebani K">K Lebani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name>
</author>
<author><name sortKey="Yu, F" uniqKey="Yu F">F Yu</name>
</author>
<author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name>
</author>
<author><name sortKey="Han, J" uniqKey="Han J">J Han</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Viranaicken, W" uniqKey="Viranaicken W">W Viranaicken</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boisen, Ml" uniqKey="Boisen M">ML Boisen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chew, Ch" uniqKey="Chew C">CH Chew</name>
</author>
<author><name sortKey="Lim, Yal" uniqKey="Lim Y">YAL Lim</name>
</author>
<author><name sortKey="Chua, Kh" uniqKey="Chua K">KH Chua</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hamidon, Nh" uniqKey="Hamidon N">NH Hamidon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rahumatullah, A" uniqKey="Rahumatullah A">A Rahumatullah</name>
</author>
<author><name sortKey="Abdul Karim, Iz" uniqKey="Abdul Karim I">IZ Abdul Karim</name>
</author>
<author><name sortKey="Noordin, R" uniqKey="Noordin R">R Noordin</name>
</author>
<author><name sortKey="Lim, Ts" uniqKey="Lim T">TS Lim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gupta, Sk" uniqKey="Gupta S">SK Gupta</name>
</author>
<author><name sortKey="Shukla, P" uniqKey="Shukla P">P Shukla</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rosano, Gl" uniqKey="Rosano G">GL Rosano</name>
</author>
<author><name sortKey="Ceccarelli, Ea" uniqKey="Ceccarelli E">EA Ceccarelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Costa, S" uniqKey="Costa S">S Costa</name>
</author>
<author><name sortKey="Almeida, A" uniqKey="Almeida A">A Almeida</name>
</author>
<author><name sortKey="Castro, A" uniqKey="Castro A">A Castro</name>
</author>
<author><name sortKey="Domingues, L" uniqKey="Domingues L">L Domingues</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jia, B" uniqKey="Jia B">B Jia</name>
</author>
<author><name sortKey="Jeon, Co" uniqKey="Jeon C">CO Jeon</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Jk" uniqKey="Liu J">JK Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Even Desrumeaux, K" uniqKey="Even Desrumeaux K">K Even-Desrumeaux</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barkhordarian, H" uniqKey="Barkhordarian H">H Barkhordarian</name>
</author>
<author><name sortKey="Emadi, S" uniqKey="Emadi S">S Emadi</name>
</author>
<author><name sortKey="Schulz, P" uniqKey="Schulz P">P Schulz</name>
</author>
<author><name sortKey="Sierks, Mr" uniqKey="Sierks M">MR Sierks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bachler, Bc" uniqKey="Bachler B">BC Bachler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Coelho, Eaf" uniqKey="Coelho E">EAF Coelho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ding, Yl" uniqKey="Ding Y">YL Ding</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, B" uniqKey="Liu B">B Liu</name>
</author>
<author><name sortKey="Huang, L" uniqKey="Huang L">L Huang</name>
</author>
<author><name sortKey="Sihlbom, C" uniqKey="Sihlbom C">C Sihlbom</name>
</author>
<author><name sortKey="Burlingame, A" uniqKey="Burlingame A">A Burlingame</name>
</author>
<author><name sortKey="Marks, Jd" uniqKey="Marks J">JD Marks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lamboy, Ja" uniqKey="Lamboy J">JA Lamboy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hammers, Cm" uniqKey="Hammers C">CM Hammers</name>
</author>
<author><name sortKey="Stanley, Jr" uniqKey="Stanley J">JR Stanley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stausb L Gr N, B" uniqKey="Stausb L Gr N B">B Stausbøl-Grøn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alfaleh, Ma" uniqKey="Alfaleh M">MA Alfaleh</name>
</author>
<author><name sortKey="Jones, Ml" uniqKey="Jones M">ML Jones</name>
</author>
<author><name sortKey="Howard, Cb" uniqKey="Howard C">CB Howard</name>
</author>
<author><name sortKey="Mahler, Sm" uniqKey="Mahler S">SM Mahler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rajaram, K" uniqKey="Rajaram K">K Rajaram</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shen, W" uniqKey="Shen W">W Shen</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, C H" uniqKey="Wu C">C-H Wu</name>
</author>
<author><name sortKey="Liu, I J" uniqKey="Liu I">I-J Liu</name>
</author>
<author><name sortKey="Lu, R M" uniqKey="Lu R">R-M Lu</name>
</author>
<author><name sortKey="Wu, H C" uniqKey="Wu H">H-C Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Adey, Nb" uniqKey="Adey N">NB Adey</name>
</author>
<author><name sortKey="Mataragnon, Ah" uniqKey="Mataragnon A">AH Mataragnon</name>
</author>
<author><name sortKey="Rider, Je" uniqKey="Rider J">JE Rider</name>
</author>
<author><name sortKey="Carter, Jm" uniqKey="Carter J">JM Carter</name>
</author>
<author><name sortKey="Kay, Bk" uniqKey="Kay B">BK Kay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bazan, J" uniqKey="Bazan J">J Bazan</name>
</author>
<author><name sortKey="Calkosi Ski, I" uniqKey="Calkosi Ski I">I Całkosiński</name>
</author>
<author><name sortKey="Gamian, A" uniqKey="Gamian A">A Gamian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hust, M" uniqKey="Hust M">M Hust</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Venet, S" uniqKey="Venet S">S Venet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bradbury, Ar" uniqKey="Bradbury A">AR Bradbury</name>
</author>
<author><name sortKey="Sidhu, S" uniqKey="Sidhu S">S Sidhu</name>
</author>
<author><name sortKey="Dubel, S" uniqKey="Dubel S">S Dübel</name>
</author>
<author><name sortKey="Mccafferty, J" uniqKey="Mccafferty J">J McCafferty</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Townsend, Cl" uniqKey="Townsend C">CL Townsend</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perfetti, V" uniqKey="Perfetti V">V Perfetti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, K" uniqKey="Smith K">K Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Boer, E" uniqKey="De Boer E">E de Boer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Humbert, N" uniqKey="Humbert N">N Humbert</name>
</author>
<author><name sortKey="Zocchi, A" uniqKey="Zocchi A">A Zocchi</name>
</author>
<author><name sortKey="Ward, Tr" uniqKey="Ward T">TR Ward</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name>
</author>
<author><name sortKey="Sousa, R" uniqKey="Sousa R">R Sousa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sorenson, Ae" uniqKey="Sorenson A">AE Sorenson</name>
</author>
<author><name sortKey="Askin, Sp" uniqKey="Askin S">SP Askin</name>
</author>
<author><name sortKey="Schaeffer, Pm" uniqKey="Schaeffer P">PM Schaeffer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fairhead, M" uniqKey="Fairhead M">M Fairhead</name>
</author>
<author><name sortKey="Howarth, M" uniqKey="Howarth M">M Howarth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jiang, L" uniqKey="Jiang L">L Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ying, T" uniqKey="Ying T">T Ying</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamaoka, Y" uniqKey="Yamaoka Y">Y Yamaoka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ying, T" uniqKey="Ying T">T Ying</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kugler, J" uniqKey="Kugler J">J Kügler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ismail, Nf" uniqKey="Ismail N">NF Ismail</name>
</author>
<author><name sortKey="Lim, Ts" uniqKey="Lim T">TS Lim</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Lim, Bn" uniqKey="Lim B">BN Lim</name>
</author>
<author><name sortKey="Chin, Cf" uniqKey="Chin C">CF Chin</name>
</author>
<author><name sortKey="Choong, Ys" uniqKey="Choong Y">YS Choong</name>
</author>
<author><name sortKey="Ismail, A" uniqKey="Ismail A">A Ismail</name>
</author>
<author><name sortKey="Lim, Ts" uniqKey="Lim T">TS Lim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brochet, X" uniqKey="Brochet X">X Brochet</name>
</author>
<author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M-P Lefranc</name>
</author>
<author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V Giudicelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="De Kruif, J" uniqKey="De Kruif J">J de Kruif</name>
</author>
<author><name sortKey="Terstappen, L" uniqKey="Terstappen L">L Terstappen</name>
</author>
<author><name sortKey="Boel, E" uniqKey="Boel E">E Boel</name>
</author>
<author><name sortKey="Logtenberg, T" uniqKey="Logtenberg T">T Logtenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Giordano, Rj" uniqKey="Giordano R">RJ Giordano</name>
</author>
<author><name sortKey="Card Vila, M" uniqKey="Card Vila M">M Cardó-Vila</name>
</author>
<author><name sortKey="Lahdenranta, J" uniqKey="Lahdenranta J">J Lahdenranta</name>
</author>
<author><name sortKey="Pasqualini, R" uniqKey="Pasqualini R">R Pasqualini</name>
</author>
<author><name sortKey="Arap, W" uniqKey="Arap W">W Arap</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Eisenhardt, Su" uniqKey="Eisenhardt S">SU Eisenhardt</name>
</author>
<author><name sortKey="Schwarz, M" uniqKey="Schwarz M">M Schwarz</name>
</author>
<author><name sortKey="Bassler, N" uniqKey="Bassler N">N Bassler</name>
</author>
<author><name sortKey="Peter, K" uniqKey="Peter K">K Peter</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="S Rensen, Md" uniqKey="S Rensen M">MD Sørensen</name>
</author>
<author><name sortKey="Agerholm, Ie" uniqKey="Agerholm I">IE Agerholm</name>
</author>
<author><name sortKey="Christensen, B" uniqKey="Christensen B">B Christensen</name>
</author>
<author><name sortKey="K Lvraa, S" uniqKey="K Lvraa S">S Kølvraa</name>
</author>
<author><name sortKey="Kristensen, P" uniqKey="Kristensen P">P Kristensen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="S Rensen, Md" uniqKey="S Rensen M">MD Sørensen</name>
</author>
<author><name sortKey="Kristensen, P" uniqKey="Kristensen P">P Kristensen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Even Desrumeaux, K" uniqKey="Even Desrumeaux K">K Even-Desrumeaux</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pavoni, E" uniqKey="Pavoni E">E Pavoni</name>
</author>
<author><name sortKey="Vaccaro, P" uniqKey="Vaccaro P">P Vaccaro</name>
</author>
<author><name sortKey="Anastasi, Am" uniqKey="Anastasi A">AM Anastasi</name>
</author>
<author><name sortKey="Minenkova, O" uniqKey="Minenkova O">O Minenkova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jones, Ml" uniqKey="Jones M">ML Jones</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002190 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002190 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:6465254 |texte= Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:30988390" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |