[Synthesis and cloning of artificial zein genes].
Identifieur interne : 001A47 ( Ncbi/Curation ); précédent : 001A46; suivant : 001A48[Synthesis and cloning of artificial zein genes].
Auteurs : V A Efimov ; A A Buriakova ; I N Pashkova ; O G ChakhmakhchevaSource :
- Bioorganicheskaia khimiia [ 0132-3423 ] ; 1988.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Zein.
- chemical : DNA Restriction Enzymes.
- genetics : Zea mays.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genes, Synthetic, Molecular Sequence Data.
Abstract
To investigate structure-function relationships in zein proteins and to develop an approach to the construction of mutant zeins of improved nutritional qualities, the chemical-enzymatic synthesis of a gene for zein cZ22B1 (22 kd) has been undertaken. This 806-base pair long DNA fragment consists of about 40 synthetic oligonucleotides, mostly 30-60-mers. The synthesis was planned with the use of a universal methodology for the artificial gene construction. The choice of appropriate sites for altering amino acid sequence and the possibility of obtaining by directed mutagenesis of the gene corresponding to modified zeins containing residues Trp and Lys in specified positions of the polypeptide chain is discussed.
PubMed: 2853634
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pubmed:2853634Le document en format XML
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