Exploration of peptides bound to MHC class I molecules in melanoma.
Identifieur interne : 001030 ( Ncbi/Curation ); précédent : 001029; suivant : 001031Exploration of peptides bound to MHC class I molecules in melanoma.
Auteurs : Antonia L. Pritchard [Australie] ; Marcus L. Hastie ; Michelle Neller ; Jeffrey J. Gorman ; Chris W. Schmidt ; Nicholas K. HaywardSource :
- Pigment cell & melanoma research [ 1755-148X ] ; 2015.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Algorithmes, Anticorps (métabolisme), Antigènes d'histocompatibilité de classe I (métabolisme), Antigènes néoplasiques (métabolisme), Données de séquences moléculaires, Fractions subcellulaires (métabolisme), Humains, Immunité, Liaison aux protéines, Lignée cellulaire tumorale, Maturation post-traductionnelle des protéines, Mélanome (génétique), Mélanome (immunologie), Peptides (), Peptides (isolement et purification), Peptides (métabolisme), Reproductibilité des résultats, Régulation de l'expression des gènes tumoraux, Spectrométrie de masse, Séquence d'acides aminés, Épitopes.
- MESH :
- génétique : ARN messager, Mélanome.
- immunologie : Mélanome.
- isolement et purification : Peptides.
- métabolisme : ARN messager, Anticorps, Antigènes d'histocompatibilité de classe I, Antigènes néoplasiques, Fractions subcellulaires, Peptides.
- Algorithmes, Données de séquences moléculaires, Humains, Immunité, Liaison aux protéines, Lignée cellulaire tumorale, Maturation post-traductionnelle des protéines, Peptides, Reproductibilité des résultats, Régulation de l'expression des gènes tumoraux, Spectrométrie de masse, Séquence d'acides aminés, Épitopes.
English descriptors
- KwdEn :
- Algorithms, Amino Acid Sequence, Antibodies (metabolism), Antigens, Neoplasm (metabolism), Cell Line, Tumor, Epitopes, Gene Expression Regulation, Neoplastic, Histocompatibility Antigens Class I (metabolism), Humans, Immunity, Mass Spectrometry, Melanoma (genetics), Melanoma (immunology), Molecular Sequence Data, Peptides (chemistry), Peptides (isolation & purification), Peptides (metabolism), Protein Binding, Protein Processing, Post-Translational, RNA, Messenger (genetics), RNA, Messenger (metabolism), Reproducibility of Results, Subcellular Fractions (metabolism).
- MESH :
- chemical , chemistry : Peptides.
- chemical , genetics : RNA, Messenger.
- chemical , isolation & purification : Peptides.
- chemical , metabolism : Antibodies, Antigens, Neoplasm, Histocompatibility Antigens Class I, Peptides, RNA, Messenger.
- genetics : Melanoma.
- immunology : Melanoma.
- metabolism : Subcellular Fractions.
- Algorithms, Amino Acid Sequence, Cell Line, Tumor, Epitopes, Gene Expression Regulation, Neoplastic, Humans, Immunity, Mass Spectrometry, Molecular Sequence Data, Protein Binding, Protein Processing, Post-Translational, Reproducibility of Results.
Abstract
Advancements in high-resolution HPLC and mass spectrometry have reinvigorated the application of this technology to identify peptides eluted from immunopurified MHC class I molecules. Three melanoma cell lines were assessed using w6/32 isolation, peptide elution and HPLC purification; peptides were identified by mass spectrometry. A total of 13,829 peptides were identified; 83-87% of these were 8-11 mers. Only approximately 15% have been described before. Subcellular locations of the source proteins showed even sampling; mRNA expression and total protein length were predictive of the number of peptides detected from a single protein. HLA-type binding prediction for 10,078 9/10 mer peptides assigned 88-95% to a patient-specific HLA subtype, revealing a disparity in strength of predicted binding. HLA-B*27-specific isolation successfully identified some peptides not found using w6/32. Sixty peptides were selected for immune screening, based on source protein and predicted HLA binding; no new peptides recognized by antimelanoma T cells were discovered. Additionally, mass spectrometry was unable to identify several epitopes targeted ex vivo by one patient's T cells.
DOI: 10.1111/pcmr.12357
PubMed: 25645385
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pubmed:25645385Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Oncogenomics Research Group, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Qld, Australia.</nlm:affiliation>
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<term>Cell Line, Tumor</term>
<term>Epitopes</term>
<term>Gene Expression Regulation, Neoplastic</term>
<term>Histocompatibility Antigens Class I (metabolism)</term>
<term>Humans</term>
<term>Immunity</term>
<term>Mass Spectrometry</term>
<term>Melanoma (genetics)</term>
<term>Melanoma (immunology)</term>
<term>Molecular Sequence Data</term>
<term>Peptides (chemistry)</term>
<term>Peptides (isolation & purification)</term>
<term>Peptides (metabolism)</term>
<term>Protein Binding</term>
<term>Protein Processing, Post-Translational</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Reproducibility of Results</term>
<term>Subcellular Fractions (metabolism)</term>
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<term>ARN messager (métabolisme)</term>
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<term>Antigènes d'histocompatibilité de classe I (métabolisme)</term>
<term>Antigènes néoplasiques (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Fractions subcellulaires (métabolisme)</term>
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<term>Immunité</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire tumorale</term>
<term>Maturation post-traductionnelle des protéines</term>
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<term>Mélanome (immunologie)</term>
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<term>Peptides (métabolisme)</term>
<term>Reproductibilité des résultats</term>
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<term>Spectrométrie de masse</term>
<term>Séquence d'acides aminés</term>
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<term>Antigens, Neoplasm</term>
<term>Histocompatibility Antigens Class I</term>
<term>Peptides</term>
<term>RNA, Messenger</term>
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<term>Anticorps</term>
<term>Antigènes d'histocompatibilité de classe I</term>
<term>Antigènes néoplasiques</term>
<term>Fractions subcellulaires</term>
<term>Peptides</term>
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<term>Amino Acid Sequence</term>
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<term>Epitopes</term>
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<term>Humans</term>
<term>Immunity</term>
<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Protein Binding</term>
<term>Protein Processing, Post-Translational</term>
<term>Reproducibility of Results</term>
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<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Immunité</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire tumorale</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Peptides</term>
<term>Reproductibilité des résultats</term>
<term>Régulation de l'expression des gènes tumoraux</term>
<term>Spectrométrie de masse</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">Advancements in high-resolution HPLC and mass spectrometry have reinvigorated the application of this technology to identify peptides eluted from immunopurified MHC class I molecules. Three melanoma cell lines were assessed using w6/32 isolation, peptide elution and HPLC purification; peptides were identified by mass spectrometry. A total of 13,829 peptides were identified; 83-87% of these were 8-11 mers. Only approximately 15% have been described before. Subcellular locations of the source proteins showed even sampling; mRNA expression and total protein length were predictive of the number of peptides detected from a single protein. HLA-type binding prediction for 10,078 9/10 mer peptides assigned 88-95% to a patient-specific HLA subtype, revealing a disparity in strength of predicted binding. HLA-B*27-specific isolation successfully identified some peptides not found using w6/32. Sixty peptides were selected for immune screening, based on source protein and predicted HLA binding; no new peptides recognized by antimelanoma T cells were discovered. Additionally, mass spectrometry was unable to identify several epitopes targeted ex vivo by one patient's T cells.</div>
</front>
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