Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)
Identifieur interne : 000E97 ( Ncbi/Curation ); précédent : 000E96; suivant : 000E98Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)
Auteurs : Nidhi Sharma ; Shuichi Hoshika ; Daniel Hutter ; Kevin M. Bradley ; Steven A. BennerSource :
- Chembiochem [ 1439-4227 ] ; 2014.
Abstract
Recombinase polymerase amplification (RPA) is an isothermal method to amplify nucleic acid sequences without the temperature cycling that classical PCR uses. Instead of using heat to denature the DNA duplex, RPA uses recombination enzymes to swap single‐stranded primers into the duplex DNA product; these are then extended using a strand‐displacing polymerase to complete the cycle. Because RPA runs at low temperatures, it never forces the system to recreate base‐pairs following Watson–Crick rules, and therefore it produces undesired products that impede the amplification of the desired product, complicating downstream analysis. Herein, we show that most of these undesired side products can be avoided if the primers contain components of a self‐avoiding molecular recognition system (SAMRS). Given the precision that is necessary in the recombination systems for them to function biologically, it is surprising that they accept SAMRS. SAMRS‐RPA is expected to be a powerful tool within the range of amplification techniques available to scientists.
Url:
DOI: 10.1002/cbic.201402250
PubMed: 25209570
PubMed Central: 7162014
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000B66
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000B66
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000F87
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000E97
Links to Exploration step
PMC:7162014Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)</title><author><name sortKey="Sharma, Nidhi" sort="Sharma, Nidhi" uniqKey="Sharma N" first="Nidhi" last="Sharma">Nidhi Sharma</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Hoshika, Shuichi" sort="Hoshika, Shuichi" uniqKey="Hoshika S" first="Shuichi" last="Hoshika">Shuichi Hoshika</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Hutter, Daniel" sort="Hutter, Daniel" uniqKey="Hutter D" first="Daniel" last="Hutter">Daniel Hutter</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Bradley, Kevin M" sort="Bradley, Kevin M" uniqKey="Bradley K" first="Kevin M." last="Bradley">Kevin M. Bradley</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Benner, Steven A" sort="Benner, Steven A" uniqKey="Benner S" first="Steven A." last="Benner">Steven A. Benner</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25209570</idno><idno type="pmc">7162014</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162014</idno><idno type="RBID">PMC:7162014</idno><idno type="doi">10.1002/cbic.201402250</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000B66</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B66</idno><idno type="wicri:Area/Pmc/Curation">000B66</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B66</idno><idno type="wicri:Area/Pmc/Checkpoint">000F87</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000F87</idno><idno type="wicri:Area/Ncbi/Merge">000E97</idno><idno type="wicri:Area/Ncbi/Curation">000E97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)</title><author><name sortKey="Sharma, Nidhi" sort="Sharma, Nidhi" uniqKey="Sharma N" first="Nidhi" last="Sharma">Nidhi Sharma</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Hoshika, Shuichi" sort="Hoshika, Shuichi" uniqKey="Hoshika S" first="Shuichi" last="Hoshika">Shuichi Hoshika</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Hutter, Daniel" sort="Hutter, Daniel" uniqKey="Hutter D" first="Daniel" last="Hutter">Daniel Hutter</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Bradley, Kevin M" sort="Bradley, Kevin M" uniqKey="Bradley K" first="Kevin M." last="Bradley">Kevin M. Bradley</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author><author><name sortKey="Benner, Steven A" sort="Benner, Steven A" uniqKey="Benner S" first="Steven A." last="Benner">Steven A. Benner</name><affiliation><nlm:aff id="aa"></nlm:aff></affiliation></author></analytic><series><title level="j">Chembiochem</title><idno type="ISSN">1439-4227</idno><idno type="eISSN">1439-7633</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Recombinase polymerase amplification (RPA) is an isothermal method to amplify nucleic acid sequences without the temperature cycling that classical PCR uses. Instead of using heat to denature the DNA duplex, RPA uses recombination enzymes to swap single‐stranded primers into the duplex DNA product; these are then extended using a strand‐displacing polymerase to complete the cycle. Because RPA runs at low temperatures, it never forces the system to recreate base‐pairs following Watson–Crick rules, and therefore it produces undesired products that impede the amplification of the desired product, complicating downstream analysis. Herein, we show that most of these undesired side products can be avoided if the primers contain components of a self‐avoiding molecular recognition system (SAMRS). Given the precision that is necessary in the recombination systems for them to function biologically, it is surprising that they accept SAMRS. SAMRS‐RPA is expected to be a powerful tool within the range of amplification techniques available to scientists.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000E97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:7162014
|texte= Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:25209570" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |