Recombinant hnRNP protein A1 and its N-terminal domain show preferential affinity for oligodeoxynucleotides homologous to intron/exon acceptor sites.
Identifieur interne : 000949 ( Ncbi/Curation ); précédent : 000948; suivant : 000950Recombinant hnRNP protein A1 and its N-terminal domain show preferential affinity for oligodeoxynucleotides homologous to intron/exon acceptor sites.
Auteurs : M. Buvoli [Italie] ; F. Cobianchi ; G. Biamonti ; S. RivaSource :
- Nucleic acids research [ 0305-1048 ] ; 1990.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Exons, Fixation compétitive, Globines (génétique), Humains, Introns, Oligodésoxyribonucléotides (), Protéines recombinantes (métabolisme), Précurseurs des ARN (), Ribonucléoprotéine nucléaire hétérogène du groupe A-B, Ribonucléoprotéines (métabolisme), Ribonucléoprotéines nucléaires hétérogènes, Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Épissage des ARN.
- MESH :
- génétique : Globines.
- métabolisme : Protéines recombinantes, Ribonucléoprotéines.
- Données de séquences moléculaires, Exons, Fixation compétitive, Humains, Introns, Oligodésoxyribonucléotides, Précurseurs des ARN, Ribonucléoprotéine nucléaire hétérogène du groupe A-B, Ribonucléoprotéines nucléaires hétérogènes, Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Épissage des ARN.
English descriptors
- KwdEn :
- Base Sequence, Binding, Competitive, Exons, Globins (genetics), Heterogeneous Nuclear Ribonucleoprotein A1, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Introns, Molecular Sequence Data, Oligodeoxyribonucleotides (chemistry), RNA Precursors (chemistry), RNA Splicing, Recombinant Proteins (metabolism), Ribonucleoproteins (metabolism), Sequence Homology, Nucleic Acid.
- MESH :
- chemical , chemistry : Oligodeoxyribonucleotides, RNA Precursors.
- chemical , genetics : Globins.
- chemical , metabolism : Recombinant Proteins, Ribonucleoproteins.
- Base Sequence, Binding, Competitive, Exons, Heterogeneous Nuclear Ribonucleoprotein A1, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Introns, Molecular Sequence Data, RNA Splicing, Sequence Homology, Nucleic Acid.
Abstract
The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to synthetic oligodeoxynucleotides (21-mers) of suitable sequence. In such a minimal system we find preferential binding of protein A1 to oligodeoxynucleotide sequences corresponding to the 3'-splice site of IVS1 of human beta-globin pre-mRNA and of IVS1 of Adenovirus type 2 major late transcript. Mutation studies demonstrate that the binding specificity is dependent on the known critical domains of this intron region, the AG splice site dinucleotide and polypyrimidine tract, and resides entirely in the short oligonucleotide sequence. Moreover specific binding does not require the presence of other hnRNP proteins or of snRNP particles. Studies with a truncated recombinant protein demonstrated that the minimal protein sequence determinants for A1 recognition of 3'-splice acceptor site reside entirely in the N-terminal 195 aa of the unmodified protein.
DOI: 10.1093/nar/18.22.6595
PubMed: 2251120
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pubmed:2251120Le document en format XML
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<term>Binding, Competitive</term>
<term>Exons</term>
<term>Globins (genetics)</term>
<term>Heterogeneous Nuclear Ribonucleoprotein A1</term>
<term>Heterogeneous-Nuclear Ribonucleoprotein Group A-B</term>
<term>Heterogeneous-Nuclear Ribonucleoproteins</term>
<term>Humans</term>
<term>Introns</term>
<term>Molecular Sequence Data</term>
<term>Oligodeoxyribonucleotides (chemistry)</term>
<term>RNA Precursors (chemistry)</term>
<term>RNA Splicing</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Ribonucleoproteins (metabolism)</term>
<term>Sequence Homology, Nucleic Acid</term>
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<term>Exons</term>
<term>Fixation compétitive</term>
<term>Globines (génétique)</term>
<term>Humains</term>
<term>Introns</term>
<term>Oligodésoxyribonucléotides ()</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Précurseurs des ARN ()</term>
<term>Ribonucléoprotéine nucléaire hétérogène du groupe A-B</term>
<term>Ribonucléoprotéines (métabolisme)</term>
<term>Ribonucléoprotéines nucléaires hétérogènes</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Séquence nucléotidique</term>
<term>Épissage des ARN</term>
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<term>RNA Precursors</term>
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<term>Ribonucleoproteins</term>
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<term>Ribonucléoprotéines</term>
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<term>Binding, Competitive</term>
<term>Exons</term>
<term>Heterogeneous Nuclear Ribonucleoprotein A1</term>
<term>Heterogeneous-Nuclear Ribonucleoprotein Group A-B</term>
<term>Heterogeneous-Nuclear Ribonucleoproteins</term>
<term>Humans</term>
<term>Introns</term>
<term>Molecular Sequence Data</term>
<term>RNA Splicing</term>
<term>Sequence Homology, Nucleic Acid</term>
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<term>Exons</term>
<term>Fixation compétitive</term>
<term>Humains</term>
<term>Introns</term>
<term>Oligodésoxyribonucléotides</term>
<term>Précurseurs des ARN</term>
<term>Ribonucléoprotéine nucléaire hétérogène du groupe A-B</term>
<term>Ribonucléoprotéines nucléaires hétérogènes</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Séquence nucléotidique</term>
<term>Épissage des ARN</term>
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<front><div type="abstract" xml:lang="en">The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to synthetic oligodeoxynucleotides (21-mers) of suitable sequence. In such a minimal system we find preferential binding of protein A1 to oligodeoxynucleotide sequences corresponding to the 3'-splice site of IVS1 of human beta-globin pre-mRNA and of IVS1 of Adenovirus type 2 major late transcript. Mutation studies demonstrate that the binding specificity is dependent on the known critical domains of this intron region, the AG splice site dinucleotide and polypyrimidine tract, and resides entirely in the short oligonucleotide sequence. Moreover specific binding does not require the presence of other hnRNP proteins or of snRNP particles. Studies with a truncated recombinant protein demonstrated that the minimal protein sequence determinants for A1 recognition of 3'-splice acceptor site reside entirely in the N-terminal 195 aa of the unmodified protein.</div>
</front>
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