Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization.
Identifieur interne : 000764 ( Ncbi/Curation ); précédent : 000763; suivant : 000765Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization.
Auteurs : Pakavadee Rakthong [Thaïlande] ; Akarin Intaramat ; Kavi RatanabanangkoonSource :
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [ 1348-2246 ] ; 2010.
Descripteurs français
- KwdFr :
- Biotinylation, Complexe antigène-anticorps (analyse), Complexe antigène-anticorps (immunologie), Diffusion de rayonnements, Hybridation d'acides nucléiques, Liposomes (), Luminol (), Lumière, Oligonucléotides (), Oligonucléotides (génétique), Phosphatidyléthanolamine (), Polyéthylène glycols (), Propriétés de surface, Protéines immobilisées (), Réaction antigène-anticorps (immunologie), Spécificité des anticorps (immunologie), Streptavidine (), Séquence nucléotidique, Techniques de biocapteur (), Études de faisabilité.
- MESH :
- analyse : Complexe antigène-anticorps.
- génétique : Oligonucléotides.
- immunologie : Complexe antigène-anticorps, Réaction antigène-anticorps, Spécificité des anticorps.
- Biotinylation, Diffusion de rayonnements, Hybridation d'acides nucléiques, Liposomes, Luminol, Lumière, Oligonucléotides, Phosphatidyléthanolamine, Polyéthylène glycols, Propriétés de surface, Protéines immobilisées, Streptavidine, Séquence nucléotidique, Techniques de biocapteur, Études de faisabilité.
English descriptors
- KwdEn :
- Antibody Specificity (immunology), Antigen-Antibody Complex (analysis), Antigen-Antibody Complex (immunology), Antigen-Antibody Reactions (immunology), Base Sequence, Biosensing Techniques (methods), Biotinylation, Feasibility Studies, Immobilized Proteins (chemistry), Light, Liposomes (chemistry), Luminol (chemistry), Nucleic Acid Hybridization, Oligonucleotides (chemistry), Oligonucleotides (genetics), Phosphatidylethanolamines (chemistry), Polyethylene Glycols (chemistry), Scattering, Radiation, Streptavidin (chemistry), Surface Properties.
- MESH :
- chemical , analysis : Antigen-Antibody Complex.
- chemical , chemistry : Immobilized Proteins, Liposomes, Luminol, Oligonucleotides, Phosphatidylethanolamines, Polyethylene Glycols, Streptavidin.
- chemical , genetics : Oligonucleotides.
- immunology : Antibody Specificity, Antigen-Antibody Complex, Antigen-Antibody Reactions.
- methods : Biosensing Techniques.
- Base Sequence, Biotinylation, Feasibility Studies, Light, Nucleic Acid Hybridization, Scattering, Radiation, Surface Properties.
Abstract
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 microM, 0.96 microM and 18 nM, respectively.
DOI: 10.2116/analsci.26.767
PubMed: 20631437
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Complex</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Immobilized Proteins</term><term>Liposomes</term><term>Luminol</term><term>Oligonucleotides</term><term>Phosphatidylethanolamines</term><term>Polyethylene Glycols</term><term>Streptavidin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Complexe antigène-anticorps</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oligonucléotides</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Complexe antigène-anticorps</term><term>Réaction antigène-anticorps</term><term>Spécificité des anticorps</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Specificity</term><term>Antigen-Antibody Complex</term><term>Antigen-Antibody 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prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 microM, 0.96 microM and 18 nM, respectively.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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