Ecdysterone regulatory elements function as both transcriptional activators and repressors.
Identifieur interne : 000730 ( Ncbi/Curation ); précédent : 000729; suivant : 000731Ecdysterone regulatory elements function as both transcriptional activators and repressors.
Auteurs : L. Dobens [États-Unis] ; K. Rudolph ; E M BergerSource :
- Molecular and cellular biology [ 0270-7306 ] ; 1991.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Clonage moléculaire, Données de séquences moléculaires, Drosophila (génétique), Ecdystérone (génétique), Ecdystérone (pharmacologie), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Fixation compétitive, Protéines du choc thermique (génétique), Régions promotrices (génétique), Régulation de l'expression des gènes, Séquence nucléotidique, Séquences d'acides nucléiques régulatrices, Transcription génétique, Transfection.
- MESH :
- génétique : Drosophila, Ecdystérone, Facteurs de transcription, Protéines du choc thermique.
- métabolisme : Facteurs de transcription.
- pharmacologie : Ecdystérone.
- Animaux, Cellules cultivées, Clonage moléculaire, Données de séquences moléculaires, Fixation compétitive, Régions promotrices (génétique), Régulation de l'expression des gènes, Séquence nucléotidique, Séquences d'acides nucléiques régulatrices, Transcription génétique, Transfection.
English descriptors
- KwdEn :
- Animals, Base Sequence, Binding, Competitive, Cells, Cultured, Cloning, Molecular, Drosophila (genetics), Ecdysterone (genetics), Ecdysterone (pharmacology), Gene Expression Regulation, Heat-Shock Proteins (genetics), Molecular Sequence Data, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Transcription Factors (genetics), Transcription Factors (metabolism), Transcription, Genetic, Transfection.
- MESH :
- chemical , genetics : Ecdysterone, Heat-Shock Proteins, Transcription Factors.
- genetics : Drosophila.
- chemical , metabolism : Transcription Factors.
- chemical , pharmacology : Ecdysterone.
- Animals, Base Sequence, Binding, Competitive, Cells, Cultured, Cloning, Molecular, Gene Expression Regulation, Molecular Sequence Data, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Transcription, Genetic, Transfection.
Abstract
A synthetic, 23-bp ecdysterone regulatory element (EcRE), derived from the upstream region of the Drosophila melanogaster hsp27 gene, was inserted adjacent to the herpes simplex virus thymidine kinase promoter fused to a bacterial gene for chloramphenicol acetyltransferase (CAT). Hybrid constructs were transfected into Drosophila S3 cells and assayed for ecdysterone-inducible CAT expression. In the absence of ecdysterone a tandem pair of EcREs repressed the high constitutive level of CAT activity found after transfection with the parent reporter plasmid alone. After hormone addition very high levels of CAT activity were observed. Insertion of the EcRE pair 3' of the CAT gene also led to high levels of ecdysterone-induced CAT expression, but the repression of high constitutive levels of CAT activity failed to occur. The EcRE-CAT construct was cotransfected with plasmids containing tandem 10-mers or 40-mers of the EcRE but lacking a reporter gene. These additional EcREs led to a reduced level of ecdysterone-induced CAT activity and to an elevation of basal CAT activity in the absence of hormone. The data suggest that the receptor binds to the EcRE in the absence of hormone, blocking basal transcription from a constitutive promoter. In the presence of ecdysterone, receptor-hormone binding to the EcRE leads to greatly enhanced transcription.
DOI: 10.1128/mcb.11.4.1846
PubMed: 2005885
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Rudolph</name></author><author><name sortKey="Berger, E M" sort="Berger, E M" uniqKey="Berger E" first="E M" last="Berger">E M Berger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:2005885</idno><idno type="pmid">2005885</idno><idno type="doi">10.1128/mcb.11.4.1846</idno><idno type="wicri:Area/PubMed/Corpus">002A01</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A01</idno><idno type="wicri:Area/PubMed/Curation">002A01</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A01</idno><idno type="wicri:Area/PubMed/Checkpoint">002844</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002844</idno><idno type="wicri:Area/Ncbi/Merge">000730</idno><idno type="wicri:Area/Ncbi/Curation">000730</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ecdysterone regulatory elements function as both transcriptional activators and repressors.</title><author><name sortKey="Dobens, L" sort="Dobens, L" uniqKey="Dobens L" first="L" last="Dobens">L. Dobens</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">New Hampshire</region></placeName><wicri:cityArea>Department of Biological Sciences, Dartmouth College, Hanover</wicri:cityArea></affiliation></author><author><name sortKey="Rudolph, K" sort="Rudolph, K" uniqKey="Rudolph K" first="K" last="Rudolph">K. Rudolph</name></author><author><name sortKey="Berger, E M" sort="Berger, E M" uniqKey="Berger E" first="E M" last="Berger">E M Berger</name></author></analytic><series><title level="j">Molecular and cellular biology</title><idno type="ISSN">0270-7306</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Binding, Competitive</term><term>Cells, Cultured</term><term>Cloning, Molecular</term><term>Drosophila (genetics)</term><term>Ecdysterone (genetics)</term><term>Ecdysterone (pharmacology)</term><term>Gene Expression Regulation</term><term>Heat-Shock Proteins (genetics)</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term><term>Regulatory Sequences, Nucleic Acid</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term><term>Transcription, Genetic</term><term>Transfection</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Drosophila (génétique)</term><term>Ecdystérone (génétique)</term><term>Ecdystérone (pharmacologie)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Fixation compétitive</term><term>Protéines du choc thermique (génétique)</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes</term><term>Séquence nucléotidique</term><term>Séquences d'acides nucléiques régulatrices</term><term>Transcription génétique</term><term>Transfection</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Ecdysterone</term><term>Heat-Shock Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drosophila</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Drosophila</term><term>Ecdystérone</term><term>Facteurs de transcription</term><term>Protéines du choc thermique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Ecdystérone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Ecdysterone</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Binding, Competitive</term><term>Cells, Cultured</term><term>Cloning, Molecular</term><term>Gene Expression Regulation</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term><term>Regulatory Sequences, Nucleic Acid</term><term>Transcription, Genetic</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Fixation compétitive</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes</term><term>Séquence nucléotidique</term><term>Séquences d'acides nucléiques régulatrices</term><term>Transcription génétique</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A synthetic, 23-bp ecdysterone regulatory element (EcRE), derived from the upstream region of the Drosophila melanogaster hsp27 gene, was inserted adjacent to the herpes simplex virus thymidine kinase promoter fused to a bacterial gene for chloramphenicol acetyltransferase (CAT). Hybrid constructs were transfected into Drosophila S3 cells and assayed for ecdysterone-inducible CAT expression. In the absence of ecdysterone a tandem pair of EcREs repressed the high constitutive level of CAT activity found after transfection with the parent reporter plasmid alone. After hormone addition very high levels of CAT activity were observed. Insertion of the EcRE pair 3' of the CAT gene also led to high levels of ecdysterone-induced CAT expression, but the repression of high constitutive levels of CAT activity failed to occur. The EcRE-CAT construct was cotransfected with plasmids containing tandem 10-mers or 40-mers of the EcRE but lacking a reporter gene. These additional EcREs led to a reduced level of ecdysterone-induced CAT activity and to an elevation of basal CAT activity in the absence of hormone. The data suggest that the receptor binds to the EcRE in the absence of hormone, blocking basal transcription from a constitutive promoter. In the presence of ecdysterone, receptor-hormone binding to the EcRE leads to greatly enhanced transcription.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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