Release of DNA oligonucleotides and their conjugates from controlled-pore glass under thermolytic conditions.
Identifieur interne : 000657 ( Ncbi/Curation ); précédent : 000656; suivant : 000658Release of DNA oligonucleotides and their conjugates from controlled-pore glass under thermolytic conditions.
Auteurs : Andrzej Grajkowski [États-Unis] ; Jacek Cie Lak ; Scott Norris ; Dar N I. Freedberg ; Jon S. Kauffman ; Robert J. Duff ; Serge L. BeaucageSource :
- Current protocols in nucleic acid chemistry [ 1934-9289 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- synthèse chimique : Oligodésoxyribonucléotides.
- Ligands, Méthodes, Oligodésoxyribonucléotides, Propriétés de surface, Réactifs réticulants, Température élevée, Verre.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- chemical , chemistry : Oligodeoxyribonucleotides.
- chemical : Cross-Linking Reagents, Ligands.
- Glass, Hot Temperature, Methods, Surface Properties.
Abstract
The sequential functionalization of long-chain alkylamine controlled-pore glass (CPG) with a 3-hydroxypropyl-(2-cyanoethyl)thiophosphoryl linker and a dinucleoside phosphorotetrazolide leads to a uniquely engineered support for solid-phase synthesis. Unlike conventional succinylated-CPG supports, this support is designed to allow oligonucleotide deprotection and elimination of deprotection side-products to proceed without release of the oligonucleotide. When needed, the DNA oligonucleotide can be thermolytically released in 2 hr under essentially neutral conditions. The modified CPG support has been successfully employed in the synthesis of both native and fully phosphorothioated DNA 20-mers. On the basis of reversed-phase HPLC and electrophoretic analyses, the purity of the released oligonucleotides is comparable to that of identical oligonucleotides synthesized from succinylated-CPG supports, in terms of both shorter-than-full-length oligonucleotide contaminants and overall yields. The detailed preparation of DNA oligonucleotides conjugated with exemplary reporter or functional groups, either at the 3'-terminus or at both 3'- and 5'-termini, is also described.
DOI: 10.1002/0471142700.nc0317s35
PubMed: 19085983
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002054
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002054
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001F63
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000657
Links to Exploration step
pubmed:19085983Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Release of DNA oligonucleotides and their conjugates from controlled-pore glass under thermolytic conditions.</title><author><name sortKey="Grajkowski, Andrzej" sort="Grajkowski, Andrzej" uniqKey="Grajkowski A" first="Andrzej" last="Grajkowski">Andrzej Grajkowski</name><affiliation wicri:level="2"><nlm:affiliation>Division of Therapeutic Proteins, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Therapeutic Proteins, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cie Lak, Jacek" sort="Cie Lak, Jacek" uniqKey="Cie Lak J" first="Jacek" last="Cie Lak">Jacek Cie Lak</name></author><author><name sortKey="Norris, Scott" sort="Norris, Scott" uniqKey="Norris S" first="Scott" last="Norris">Scott Norris</name></author><author><name sortKey="Freedberg, Dar N I" sort="Freedberg, Dar N I" uniqKey="Freedberg D" first="Dar N I" last="Freedberg">Dar N I. Freedberg</name></author><author><name sortKey="Kauffman, Jon S" sort="Kauffman, Jon S" uniqKey="Kauffman J" first="Jon S" last="Kauffman">Jon S. Kauffman</name></author><author><name sortKey="Duff, Robert J" sort="Duff, Robert J" uniqKey="Duff R" first="Robert J" last="Duff">Robert J. Duff</name></author><author><name sortKey="Beaucage, Serge L" sort="Beaucage, Serge L" uniqKey="Beaucage S" first="Serge L" last="Beaucage">Serge L. Beaucage</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:19085983</idno><idno type="pmid">19085983</idno><idno type="doi">10.1002/0471142700.nc0317s35</idno><idno type="wicri:Area/PubMed/Corpus">002054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002054</idno><idno type="wicri:Area/PubMed/Curation">002054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002054</idno><idno type="wicri:Area/PubMed/Checkpoint">001F63</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F63</idno><idno type="wicri:Area/Ncbi/Merge">000657</idno><idno type="wicri:Area/Ncbi/Curation">000657</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Release of DNA oligonucleotides and their conjugates from controlled-pore glass under thermolytic conditions.</title><author><name sortKey="Grajkowski, Andrzej" sort="Grajkowski, Andrzej" uniqKey="Grajkowski A" first="Andrzej" last="Grajkowski">Andrzej Grajkowski</name><affiliation wicri:level="2"><nlm:affiliation>Division of Therapeutic Proteins, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Therapeutic Proteins, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cie Lak, Jacek" sort="Cie Lak, Jacek" uniqKey="Cie Lak J" first="Jacek" last="Cie Lak">Jacek Cie Lak</name></author><author><name sortKey="Norris, Scott" sort="Norris, Scott" uniqKey="Norris S" first="Scott" last="Norris">Scott Norris</name></author><author><name sortKey="Freedberg, Dar N I" sort="Freedberg, Dar N I" uniqKey="Freedberg D" first="Dar N I" last="Freedberg">Dar N I. Freedberg</name></author><author><name sortKey="Kauffman, Jon S" sort="Kauffman, Jon S" uniqKey="Kauffman J" first="Jon S" last="Kauffman">Jon S. Kauffman</name></author><author><name sortKey="Duff, Robert J" sort="Duff, Robert J" uniqKey="Duff R" first="Robert J" last="Duff">Robert J. Duff</name></author><author><name sortKey="Beaucage, Serge L" sort="Beaucage, Serge L" uniqKey="Beaucage S" first="Serge L" last="Beaucage">Serge L. Beaucage</name></author></analytic><series><title level="j">Current protocols in nucleic acid chemistry</title><idno type="eISSN">1934-9289</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cross-Linking Reagents</term><term>Glass</term><term>Hot Temperature</term><term>Ligands</term><term>Methods</term><term>Oligodeoxyribonucleotides (chemical synthesis)</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Surface Properties</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ligands</term><term>Méthodes</term><term>Oligodésoxyribonucléotides ()</term><term>Oligodésoxyribonucléotides (synthèse chimique)</term><term>Propriétés de surface</term><term>Réactifs réticulants</term><term>Température élevée</term><term>Verre</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cross-Linking Reagents</term><term>Ligands</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Glass</term><term>Hot Temperature</term><term>Methods</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Ligands</term><term>Méthodes</term><term>Oligodésoxyribonucléotides</term><term>Propriétés de surface</term><term>Réactifs réticulants</term><term>Température élevée</term><term>Verre</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The sequential functionalization of long-chain alkylamine controlled-pore glass (CPG) with a 3-hydroxypropyl-(2-cyanoethyl)thiophosphoryl linker and a dinucleoside phosphorotetrazolide leads to a uniquely engineered support for solid-phase synthesis. Unlike conventional succinylated-CPG supports, this support is designed to allow oligonucleotide deprotection and elimination of deprotection side-products to proceed without release of the oligonucleotide. When needed, the DNA oligonucleotide can be thermolytically released in 2 hr under essentially neutral conditions. The modified CPG support has been successfully employed in the synthesis of both native and fully phosphorothioated DNA 20-mers. On the basis of reversed-phase HPLC and electrophoretic analyses, the purity of the released oligonucleotides is comparable to that of identical oligonucleotides synthesized from succinylated-CPG supports, in terms of both shorter-than-full-length oligonucleotide contaminants and overall yields. The detailed preparation of DNA oligonucleotides conjugated with exemplary reporter or functional groups, either at the 3'-terminus or at both 3'- and 5'-termini, is also described.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000657 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000657 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= pubmed:19085983
|texte= Release of DNA oligonucleotides and their conjugates from controlled-pore glass under thermolytic conditions.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:19085983" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |