A barbiturate-regulated protein binding to a common sequence in the cytochrome P450 genes of rodents and bacteria.
Identifieur interne : 000652 ( Ncbi/Curation ); précédent : 000651; suivant : 000653A barbiturate-regulated protein binding to a common sequence in the cytochrome P450 genes of rodents and bacteria.
Auteurs : J S He ; A J FulcoSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 1991.
Descripteurs français
- KwdFr :
- Animaux, Bacillus megaterium (enzymologie), Barbituriques (pharmacologie), Chloramphenicol O-acetyltransferase (génétique), Cytochrome P-450 enzyme system (biosynthèse), Cytochrome P-450 enzyme system (génétique), Données de séquences moléculaires, Foie (enzymologie), Induction enzymatique, Lignées consanguines de rats, Plasmides, Rats, Régulation de l'expression des gènes codant pour des enzymes, Séquences d'acides nucléiques régulatrices.
- MESH :
- biosynthèse : Cytochrome P-450 enzyme system.
- enzymologie : Bacillus megaterium, Foie.
- génétique : Chloramphenicol O-acetyltransferase, Cytochrome P-450 enzyme system.
- pharmacologie : Barbituriques.
- Animaux, Données de séquences moléculaires, Induction enzymatique, Lignées consanguines de rats, Plasmides, Rats, Régulation de l'expression des gènes codant pour des enzymes, Séquences d'acides nucléiques régulatrices.
English descriptors
- KwdEn :
- Animals, Bacillus megaterium (enzymology), Barbiturates (pharmacology), Chloramphenicol O-Acetyltransferase (genetics), Cytochrome P-450 Enzyme System (biosynthesis), Cytochrome P-450 Enzyme System (genetics), Enzyme Induction, Gene Expression Regulation, Enzymologic, Liver (enzymology), Molecular Sequence Data, Plasmids, Rats, Rats, Inbred Strains, Regulatory Sequences, Nucleic Acid.
- MESH :
- chemical , biosynthesis : Cytochrome P-450 Enzyme System.
- chemical , genetics : Chloramphenicol O-Acetyltransferase, Cytochrome P-450 Enzyme System.
- chemical , pharmacology : Barbiturates.
- enzymology : Bacillus megaterium, Liver.
- Animals, Enzyme Induction, Gene Expression Regulation, Enzymologic, Molecular Sequence Data, Plasmids, Rats, Rats, Inbred Strains, Regulatory Sequences, Nucleic Acid.
Abstract
Analyses of the 5' regulatory sequences of genes encoding barbiturate-inducible cytochromes P450BM-1 and P450BM-3 from Bacillus megaterium and of the 5' sequences of genes for barbiturate-inducible P450b and P450e of the rat revealed a string of 17 base pairs in each of the genes that shared a high degree of sequence identity. Labeled oligonucleotide probes of each of these four sequences were tested in gel retardation assays with protein obtained from B. megaterium grown either in the presence or absence of barbiturates or with protein from nuclear extracts from livers of rats left untreated or injected with phenobarbital. Each of the four 17-mers bound strongly to a single protein from bacteria grown in the absence of barbiturates, but this binding was dramatically reduced with protein from pentobarbital- or phenobarbital-grown cells. Conversely, the probes complexed weakly to one protein band from nuclear extracts from untreated rats but much more strongly with protein from phenobarbital-treated rats. Similar effects could be obtained by prolonged incubation with phenobarbital of either soluble protein from the bacteria grown in the absence of barbiturates or nuclear extract protein from untreated rats. Deletion analysis of the 5'-flanking region of the P450BM-1 gene of B. megaterium revealed a putative repressor binding site located within a 24-base pair DNA segment that included the 17-base pair sequence involved in barbiturate-regulated protein binding.
PubMed: 1902228
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J S He<affiliation><nlm:affiliation>Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024-1737.</nlm:affiliation><wicri:noCountry code="subField">Los Angeles 90024-1737</wicri:noCountry></affiliation>Le document en format XML
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last="Fulco">A J Fulco</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bacillus megaterium (enzymology)</term><term>Barbiturates (pharmacology)</term><term>Chloramphenicol O-Acetyltransferase (genetics)</term><term>Cytochrome P-450 Enzyme System (biosynthesis)</term><term>Cytochrome P-450 Enzyme System (genetics)</term><term>Enzyme Induction</term><term>Gene Expression Regulation, Enzymologic</term><term>Liver (enzymology)</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Rats</term><term>Rats, Inbred Strains</term><term>Regulatory Sequences, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bacillus megaterium (enzymologie)</term><term>Barbituriques (pharmacologie)</term><term>Chloramphenicol O-acetyltransferase (génétique)</term><term>Cytochrome P-450 enzyme system (biosynthèse)</term><term>Cytochrome P-450 enzyme system (génétique)</term><term>Données de séquences moléculaires</term><term>Foie (enzymologie)</term><term>Induction enzymatique</term><term>Lignées consanguines de rats</term><term>Plasmides</term><term>Rats</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Séquences d'acides nucléiques régulatrices</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chloramphenicol O-Acetyltransferase</term><term>Cytochrome P-450 Enzyme System</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Barbiturates</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cytochrome P-450 enzyme system</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bacillus megaterium</term><term>Foie</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacillus megaterium</term><term>Liver</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chloramphenicol O-acetyltransferase</term><term>Cytochrome P-450 enzyme system</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Barbituriques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme Induction</term><term>Gene Expression Regulation, Enzymologic</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Rats</term><term>Rats, Inbred Strains</term><term>Regulatory Sequences, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Induction enzymatique</term><term>Lignées consanguines de rats</term><term>Plasmides</term><term>Rats</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Séquences d'acides nucléiques régulatrices</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Analyses of the 5' regulatory sequences of genes encoding barbiturate-inducible cytochromes P450BM-1 and P450BM-3 from Bacillus megaterium and of the 5' sequences of genes for barbiturate-inducible P450b and P450e of the rat revealed a string of 17 base pairs in each of the genes that shared a high degree of sequence identity. Labeled oligonucleotide probes of each of these four sequences were tested in gel retardation assays with protein obtained from B. megaterium grown either in the presence or absence of barbiturates or with protein from nuclear extracts from livers of rats left untreated or injected with phenobarbital. Each of the four 17-mers bound strongly to a single protein from bacteria grown in the absence of barbiturates, but this binding was dramatically reduced with protein from pentobarbital- or phenobarbital-grown cells. Conversely, the probes complexed weakly to one protein band from nuclear extracts from untreated rats but much more strongly with protein from phenobarbital-treated rats. Similar effects could be obtained by prolonged incubation with phenobarbital of either soluble protein from the bacteria grown in the absence of barbiturates or nuclear extract protein from untreated rats. Deletion analysis of the 5'-flanking region of the P450BM-1 gene of B. megaterium revealed a putative repressor binding site located within a 24-base pair DNA segment that included the 17-base pair sequence involved in barbiturate-regulated protein binding.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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