Improving Phrap-Based Assembly of the Rat Using “Reliable” Overlaps
Identifieur interne : 000591 ( Ncbi/Curation ); précédent : 000590; suivant : 000592Improving Phrap-Based Assembly of the Rat Using “Reliable” Overlaps
Auteurs : Michael Roberts [États-Unis] ; Aleksey V. Zimin [États-Unis] ; Wayne Hayes [États-Unis] ; Brian R. Hunt [États-Unis] ; Cevat Ustun [États-Unis] ; James R. White [États-Unis] ; Paul Havlak [États-Unis] ; James Yorke [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Rats.
- Animaux, Chromosomes artificiels de bactérie, Génome, Reproductibilité des résultats.
English descriptors
- KwdEn :
- MESH :
- genetics : Rats.
- Animals, Chromosomes, Artificial, Bacterial, Genome, Reproducibility of Results.
Abstract
The assembly methods used for whole-genome shotgun (WGS) data have a major impact on the quality of resulting draft genomes. We present a novel algorithm to generate a set of “reliable” overlaps based on identifying repeat k-mers. To demonstrate the benefits of using reliable overlaps, we have created a version of the Phrap assembly program that uses only overlaps from a specific list. We call this version
Url:
DOI: 10.1371/journal.pone.0001836
PubMed: 18350171
PubMed Central: 2266800
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PMC:2266800Le document en format XML
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<term>Chromosomes, Artificial, Bacterial</term>
<term>Genome</term>
<term>Rats (genetics)</term>
<term>Reproducibility of Results</term>
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<term>Chromosomes artificiels de bactérie</term>
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<term>Rats (génétique)</term>
<term>Reproductibilité des résultats</term>
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<term>Genome</term>
<term>Reproducibility of Results</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chromosomes artificiels de bactérie</term>
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<front><div type="abstract" xml:lang="en"><p>The assembly methods used for whole-genome shotgun (WGS) data have a major impact on the quality of resulting draft genomes. We present a novel algorithm to generate a set of “reliable” overlaps based on identifying repeat k-mers. To demonstrate the benefits of using reliable overlaps, we have created a version of the Phrap assembly program that uses only overlaps from a specific list. We call this version <italic>PhrapUMD</italic>
. Integrating PhrapUMD and our “reliable-overlap” algorithm with the Baylor College of Medicine assembler, Atlas, we assemble the BACs from the <italic>Rattus norvegicus</italic>
genome project. Starting with the same data as the Nov. 2002 Atlas assembly, we compare our results and the Atlas assembly to the 4.3 Mb of rat sequence in the 21 BACs that have been finished. Our version of the draft assembly of the 21 BACs increases the coverage of finished sequence from 93.4% to 96.3%, while simultaneously reducing the base error rate from 4.5 to 1.1 errors per 10,000 bases. There are a number of ways of assessing the relative merits of assemblies when the finished sequence is available. If one views the overall quality of an assembly as proportional to the inverse of the product of the error rate and sequence missed, then the assembly presented here is seven times better. The UMD Overlapper with options for reliable overlaps is available from the authors at <ext-link ext-link-type="uri" xlink:href="http://www.genome.umd.edu">http://www.genome.umd.edu</ext-link>
. We also provide the changes to the Phrap source code enabling it to use only the reliable overlaps.</p>
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</author>
<author><name sortKey="Egan, A" uniqKey="Egan A">A Egan</name>
</author>
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<author><name sortKey="Butler, J" uniqKey="Butler J">J Butler</name>
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<author><name sortKey="Stupka, E" uniqKey="Stupka E">E Stupka</name>
</author>
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<author><name sortKey="Chia, Jm" uniqKey="Chia J">JM Chia</name>
</author>
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</author>
<author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name>
</author>
<author><name sortKey="Aluru, S" uniqKey="Aluru S">S Aluru</name>
</author>
<author><name sortKey="Yang, Sp" uniqKey="Yang S">SP Yang</name>
</author>
<author><name sortKey="Hillier, L" uniqKey="Hillier L">L Hillier</name>
</author>
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</author>
</analytic>
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</author>
<author><name sortKey="Smit, A" uniqKey="Smit A">A Smit</name>
</author>
<author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name>
</author>
<author><name sortKey="Baertsch, R" uniqKey="Baertsch R">R Baertsch</name>
</author>
</analytic>
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</author>
<author><name sortKey="Peterson, J" uniqKey="Peterson J">J Peterson</name>
</author>
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