Detection of oligosaccharide ligands for hepatocyte growth factor/scatter factor (HGF/SF), keratinocyte growth factor (KGF/FGF-7), RANTES and heparin cofactor II by neoglycolipid microarrays of glycosaminoglycan-derived oligosaccharide fragments.
Identifieur interne : 000472 ( Ncbi/Curation ); précédent : 000471; suivant : 000473Detection of oligosaccharide ligands for hepatocyte growth factor/scatter factor (HGF/SF), keratinocyte growth factor (KGF/FGF-7), RANTES and heparin cofactor II by neoglycolipid microarrays of glycosaminoglycan-derived oligosaccharide fragments.
Auteurs : Keiko Yamaguchi [Japon] ; Hirotoshi Tamaki ; Shigeyuki FukuiSource :
- Glycoconjugate journal [ 0282-0080 ] ; 2006.
Descripteurs français
- KwdFr :
- Analyse sur microréseau (), Animaux, Bovins, Chimiokine CCL5 (métabolisme), Chondroitinases et chondroitin lyases, Cofacteur II de l'héparine (métabolisme), Colorants fluorescents, Facteur de croissance des hépatocytes (métabolisme), Facteur de croissance fibroblastique de type 7 (métabolisme), Glycosaminoglycanes (), Glycosaminoglycanes (métabolisme), Heparin lyase, Humains, Héparine (), Héparine (métabolisme), Lapins, Liaison aux protéines, Ligands, Oligosaccharides (), Oligosaccharides (métabolisme), Protéines recombinantes (métabolisme), Souris, Techniques in vitro.
- MESH :
- métabolisme : Chimiokine CCL5, Cofacteur II de l'héparine, Facteur de croissance des hépatocytes, Facteur de croissance fibroblastique de type 7, Glycosaminoglycanes, Héparine, Oligosaccharides, Protéines recombinantes.
- Analyse sur microréseau, Animaux, Bovins, Chondroitinases et chondroitin lyases, Colorants fluorescents, Glycosaminoglycanes, Heparin lyase, Humains, Héparine, Lapins, Liaison aux protéines, Ligands, Oligosaccharides, Souris, Techniques in vitro.
English descriptors
- KwdEn :
- Animals, Cattle, Chemokine CCL5 (metabolism), Chondroitinases and Chondroitin Lyases, Fibroblast Growth Factor 7 (metabolism), Fluorescent Dyes, Glycosaminoglycans (chemistry), Glycosaminoglycans (metabolism), Heparin (chemistry), Heparin (metabolism), Heparin Cofactor II (metabolism), Heparin Lyase, Hepatocyte Growth Factor (metabolism), Humans, In Vitro Techniques, Ligands, Mice, Microarray Analysis (methods), Oligosaccharides (chemistry), Oligosaccharides (metabolism), Protein Binding, Rabbits, Recombinant Proteins (metabolism).
- MESH :
- chemical , chemistry : Glycosaminoglycans, Heparin, Oligosaccharides.
- chemical , metabolism : Chemokine CCL5, Fibroblast Growth Factor 7, Glycosaminoglycans, Heparin, Heparin Cofactor II, Hepatocyte Growth Factor, Oligosaccharides, Recombinant Proteins.
- methods : Microarray Analysis.
- Animals, Cattle, Chondroitinases and Chondroitin Lyases, Fluorescent Dyes, Heparin Lyase, Humans, In Vitro Techniques, Ligands, Mice, Protein Binding, Rabbits.
Abstract
Neoglycolipid technology is eminently adaptable for microarray design for high-throughput detection and specificity assignments of carbohydrate-protein interactions. Dermatan sulfate (DS) is known to play an important role because of its ability to bind growth factors as well as chemokines and to modulate their biological activities during inflammation and response to injury. We prepared various iduronic acid-rich fragments from DS by complete digestion with chondroitinase ACI, and investigated whether the DS-binding proteins, such as HGF/SF, RANTES, KGF/FGF-7 and HCII, can detect their oligosaccharide ligands in a neoglycolipid microarray. First, a comparison of the intensity of binding signals obtained from chondroitin oligosaccharides with those of heparin oligosaccharides showed that our microarray system is feasible not only to single-out the oligosaccharide ligands, but also to detect the difference between an intrinsic interaction unrelated only to electrostatic interaction and non-specific electrostatic interaction. Second, HGF/SF, KGF/FGF-7 and HCII showed preferential binding to iduronic acid-rich fragments of DS oligosaccharides that are greater than 8-mers in lengths. In contrast, RANTES binding seemed to depend only on the negative charges; their binding intensity towards the DS oligosaccharides was somewhat stronger than the binding of HGF/SF, KGF/FGF-7 and HCII. Third, the use of polyvinylpyrrolidone-40 (PVP-40), ovalbumin (OV) and Tween 20 in place of BSA as a blotting agent was useful in these glycosaminoglycan dependent reactions to minimize background due to non-specific interactions.
DOI: 10.1007/s10719-006-7151-z
PubMed: 17006643
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pubmed:17006643Le document en format XML
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last="Tamaki">Hirotoshi Tamaki</name></author><author><name sortKey="Fukui, Shigeyuki" sort="Fukui, Shigeyuki" uniqKey="Fukui S" first="Shigeyuki" last="Fukui">Shigeyuki Fukui</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17006643</idno><idno type="pmid">17006643</idno><idno type="doi">10.1007/s10719-006-7151-z</idno><idno type="wicri:Area/PubMed/Corpus">002221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002221</idno><idno type="wicri:Area/PubMed/Curation">002221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002221</idno><idno type="wicri:Area/PubMed/Checkpoint">002141</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002141</idno><idno type="wicri:Area/Ncbi/Merge">000472</idno><idno 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sort="Tamaki, Hirotoshi" uniqKey="Tamaki H" first="Hirotoshi" last="Tamaki">Hirotoshi Tamaki</name></author><author><name sortKey="Fukui, Shigeyuki" sort="Fukui, Shigeyuki" uniqKey="Fukui S" first="Shigeyuki" last="Fukui">Shigeyuki Fukui</name></author></analytic><series><title level="j">Glycoconjugate journal</title><idno type="ISSN">0282-0080</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Chemokine CCL5 (metabolism)</term><term>Chondroitinases and Chondroitin Lyases</term><term>Fibroblast Growth Factor 7 (metabolism)</term><term>Fluorescent Dyes</term><term>Glycosaminoglycans (chemistry)</term><term>Glycosaminoglycans (metabolism)</term><term>Heparin (chemistry)</term><term>Heparin (metabolism)</term><term>Heparin Cofactor II (metabolism)</term><term>Heparin Lyase</term><term>Hepatocyte Growth Factor (metabolism)</term><term>Humans</term><term>In Vitro Techniques</term><term>Ligands</term><term>Mice</term><term>Microarray Analysis (methods)</term><term>Oligosaccharides (chemistry)</term><term>Oligosaccharides (metabolism)</term><term>Protein Binding</term><term>Rabbits</term><term>Recombinant Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse sur microréseau ()</term><term>Animaux</term><term>Bovins</term><term>Chimiokine CCL5 (métabolisme)</term><term>Chondroitinases et chondroitin lyases</term><term>Cofacteur II de l'héparine (métabolisme)</term><term>Colorants fluorescents</term><term>Facteur de croissance des hépatocytes (métabolisme)</term><term>Facteur de croissance fibroblastique de type 7 (métabolisme)</term><term>Glycosaminoglycanes ()</term><term>Glycosaminoglycanes (métabolisme)</term><term>Heparin lyase</term><term>Humains</term><term>Héparine ()</term><term>Héparine (métabolisme)</term><term>Lapins</term><term>Liaison aux protéines</term><term>Ligands</term><term>Oligosaccharides ()</term><term>Oligosaccharides (métabolisme)</term><term>Protéines recombinantes (métabolisme)</term><term>Souris</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glycosaminoglycans</term><term>Heparin</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chemokine CCL5</term><term>Fibroblast Growth Factor 7</term><term>Glycosaminoglycans</term><term>Heparin</term><term>Heparin Cofactor II</term><term>Hepatocyte Growth Factor</term><term>Oligosaccharides</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microarray Analysis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chimiokine CCL5</term><term>Cofacteur II de l'héparine</term><term>Facteur de croissance des hépatocytes</term><term>Facteur de croissance fibroblastique de type 7</term><term>Glycosaminoglycanes</term><term>Héparine</term><term>Oligosaccharides</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Chondroitinases and Chondroitin Lyases</term><term>Fluorescent Dyes</term><term>Heparin Lyase</term><term>Humans</term><term>In Vitro Techniques</term><term>Ligands</term><term>Mice</term><term>Protein Binding</term><term>Rabbits</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur microréseau</term><term>Animaux</term><term>Bovins</term><term>Chondroitinases et chondroitin lyases</term><term>Colorants fluorescents</term><term>Glycosaminoglycanes</term><term>Heparin lyase</term><term>Humains</term><term>Héparine</term><term>Lapins</term><term>Liaison aux protéines</term><term>Ligands</term><term>Oligosaccharides</term><term>Souris</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Neoglycolipid technology is eminently adaptable for microarray design for high-throughput detection and specificity assignments of carbohydrate-protein interactions. Dermatan sulfate (DS) is known to play an important role because of its ability to bind growth factors as well as chemokines and to modulate their biological activities during inflammation and response to injury. We prepared various iduronic acid-rich fragments from DS by complete digestion with chondroitinase ACI, and investigated whether the DS-binding proteins, such as HGF/SF, RANTES, KGF/FGF-7 and HCII, can detect their oligosaccharide ligands in a neoglycolipid microarray. First, a comparison of the intensity of binding signals obtained from chondroitin oligosaccharides with those of heparin oligosaccharides showed that our microarray system is feasible not only to single-out the oligosaccharide ligands, but also to detect the difference between an intrinsic interaction unrelated only to electrostatic interaction and non-specific electrostatic interaction. Second, HGF/SF, KGF/FGF-7 and HCII showed preferential binding to iduronic acid-rich fragments of DS oligosaccharides that are greater than 8-mers in lengths. In contrast, RANTES binding seemed to depend only on the negative charges; their binding intensity towards the DS oligosaccharides was somewhat stronger than the binding of HGF/SF, KGF/FGF-7 and HCII. Third, the use of polyvinylpyrrolidone-40 (PVP-40), ovalbumin (OV) and Tween 20 in place of BSA as a blotting agent was useful in these glycosaminoglycan dependent reactions to minimize background due to non-specific interactions.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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