RNA interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle system.
Identifieur interne : 000447 ( Ncbi/Curation ); précédent : 000446; suivant : 000448RNA interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle system.
Auteurs : Kenneth A. Howard [Danemark] ; Ulrik L. Rahbek ; Xiudong Liu ; Christian K. Damgaard ; Sys Zoffmann Glud ; Morten Andersen ; Mads B. Hovgaard ; Alexander Schmitz ; Jens R. Nyengaard ; Flemming Besenbacher ; J Rgen KjemsSource :
- Molecular therapy : the journal of the American Society of Gene Therapy [ 1525-0016 ] ; 2006.
Descripteurs français
- KwdFr :
- Analyse spectrale, Animaux, Cellules cultivées, Chimie physique, Chitosane (), Gènes rapporteurs (génétique), Humains, Interférence par ARN, Microscopie à force atomique, Nanostructures (), Nanostructures (ultrastructure), Petit ARN interférent (), Petit ARN interférent (génétique), Phénomènes chimiques, Poumon (métabolisme), Souris.
- MESH :
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Chemical Phenomena, Chemistry, Physical, Chitosan (chemistry), Genes, Reporter (genetics), Humans, Lung (metabolism), Mice, Microscopy, Atomic Force, Nanostructures (chemistry), Nanostructures (ultrastructure), RNA Interference, RNA, Small Interfering (chemistry), RNA, Small Interfering (genetics), Spectrum Analysis.
- MESH :
- chemical , chemistry : Chitosan, RNA, Small Interfering.
- chemistry : Nanostructures.
- genetics : Genes, Reporter, RNA, Small Interfering.
- metabolism : Lung.
- ultrastructure : Nanostructures.
- Animals, Cells, Cultured, Chemical Phenomena, Chemistry, Physical, Humans, Mice, Microscopy, Atomic Force, RNA Interference, Spectrum Analysis.
Abstract
This work introduces a novel chitosan-based siRNA nanoparticle delivery system for RNA interference in vitro and in vivo. The formation of interpolyelectrolyte complexes between siRNA duplexes (21-mers) and chitosan polymer into nanoparticles, ranging from 40 to 600 nm, was shown using atomic force microscopy and photon correlation spectroscopy. Rapid uptake (1 h) of Cy5-labeled nanoparticles into NIH 3T3 cells, followed by accumulation over a 24 h period, was visualized using fluorescence microscopy. Nanoparticle-mediated knockdown of endogenous enhanced green fluorescent protein (EGFP) was demonstrated in both H1299 human lung carcinoma cells and murine peritoneal macrophages (77.9% and 89.3% reduction in EGFP fluorescence, respectively). In addition, Western analysis showed approximately 90% reduced expression of BCR/ABL-1 leukemia fusion protein while BCR expression was unaffected in K562 (Ph(+)) cells after transfection using nanoparticles containing siRNA specific to the BCR/ABL-1 junction sequence. Effective in vivo RNA interference was achieved in bronchiole epithelial cells of transgenic EGFP mice after nasal administration of chitosan/siRNA formulations (37% and 43% reduction compared to mismatch and untreated control, respectively). These findings highlight the potential application of this novel chitosan-based system in RNA-mediated therapy of systemic and mucosal disease.
DOI: 10.1016/j.ymthe.2006.04.010
PubMed: 16829204
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pubmed:16829204Le document en format XML
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Rahbek</name></author><author><name sortKey="Liu, Xiudong" sort="Liu, Xiudong" uniqKey="Liu X" first="Xiudong" last="Liu">Xiudong Liu</name></author><author><name sortKey="Damgaard, Christian K" sort="Damgaard, Christian K" uniqKey="Damgaard C" first="Christian K" last="Damgaard">Christian K. Damgaard</name></author><author><name sortKey="Glud, Sys Zoffmann" sort="Glud, Sys Zoffmann" uniqKey="Glud S" first="Sys Zoffmann" last="Glud">Sys Zoffmann Glud</name></author><author><name sortKey="Andersen, Morten" sort="Andersen, Morten" uniqKey="Andersen M" first="Morten" last="Andersen">Morten Andersen</name></author><author><name sortKey="Hovgaard, Mads B" sort="Hovgaard, Mads B" uniqKey="Hovgaard M" first="Mads B" last="Hovgaard">Mads B. 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Howard</name><affiliation wicri:level="1"><nlm:affiliation>Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, 8000 Aarhus C, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, 8000 Aarhus C</wicri:regionArea><wicri:noRegion>8000 Aarhus C</wicri:noRegion></affiliation></author><author><name sortKey="Rahbek, Ulrik L" sort="Rahbek, Ulrik L" uniqKey="Rahbek U" first="Ulrik L" last="Rahbek">Ulrik L. Rahbek</name></author><author><name sortKey="Liu, Xiudong" sort="Liu, Xiudong" uniqKey="Liu X" first="Xiudong" last="Liu">Xiudong Liu</name></author><author><name sortKey="Damgaard, Christian K" sort="Damgaard, Christian K" uniqKey="Damgaard C" first="Christian K" last="Damgaard">Christian K. Damgaard</name></author><author><name sortKey="Glud, Sys Zoffmann" sort="Glud, Sys Zoffmann" uniqKey="Glud S" first="Sys Zoffmann" last="Glud">Sys Zoffmann Glud</name></author><author><name sortKey="Andersen, Morten" sort="Andersen, Morten" uniqKey="Andersen M" first="Morten" last="Andersen">Morten Andersen</name></author><author><name sortKey="Hovgaard, Mads B" sort="Hovgaard, Mads B" uniqKey="Hovgaard M" first="Mads B" last="Hovgaard">Mads B. Hovgaard</name></author><author><name sortKey="Schmitz, Alexander" sort="Schmitz, Alexander" uniqKey="Schmitz A" first="Alexander" last="Schmitz">Alexander Schmitz</name></author><author><name sortKey="Nyengaard, Jens R" sort="Nyengaard, Jens R" uniqKey="Nyengaard J" first="Jens R" last="Nyengaard">Jens R. Nyengaard</name></author><author><name sortKey="Besenbacher, Flemming" sort="Besenbacher, Flemming" uniqKey="Besenbacher F" first="Flemming" last="Besenbacher">Flemming Besenbacher</name></author><author><name sortKey="Kjems, J Rgen" sort="Kjems, J Rgen" uniqKey="Kjems J" first="J Rgen" last="Kjems">J Rgen Kjems</name></author></analytic><series><title level="j">Molecular therapy : the journal of the American Society of Gene Therapy</title><idno type="ISSN">1525-0016</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chemical Phenomena</term><term>Chemistry, Physical</term><term>Chitosan (chemistry)</term><term>Genes, Reporter (genetics)</term><term>Humans</term><term>Lung (metabolism)</term><term>Mice</term><term>Microscopy, Atomic Force</term><term>Nanostructures (chemistry)</term><term>Nanostructures (ultrastructure)</term><term>RNA Interference</term><term>RNA, Small Interfering (chemistry)</term><term>RNA, Small Interfering (genetics)</term><term>Spectrum Analysis</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse spectrale</term><term>Animaux</term><term>Cellules cultivées</term><term>Chimie physique</term><term>Chitosane ()</term><term>Gènes rapporteurs (génétique)</term><term>Humains</term><term>Interférence par ARN</term><term>Microscopie à force atomique</term><term>Nanostructures ()</term><term>Nanostructures (ultrastructure)</term><term>Petit ARN interférent ()</term><term>Petit ARN interférent (génétique)</term><term>Phénomènes chimiques</term><term>Poumon (métabolisme)</term><term>Souris</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chitosan</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Reporter</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gènes rapporteurs</term><term>Petit ARN interférent</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chemical Phenomena</term><term>Chemistry, Physical</term><term>Humans</term><term>Mice</term><term>Microscopy, Atomic Force</term><term>RNA Interference</term><term>Spectrum Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse spectrale</term><term>Animaux</term><term>Cellules cultivées</term><term>Chimie physique</term><term>Chitosane</term><term>Humains</term><term>Interférence par ARN</term><term>Microscopie à force atomique</term><term>Nanostructures</term><term>Petit ARN interférent</term><term>Phénomènes chimiques</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This work introduces a novel chitosan-based siRNA nanoparticle delivery system for RNA interference in vitro and in vivo. The formation of interpolyelectrolyte complexes between siRNA duplexes (21-mers) and chitosan polymer into nanoparticles, ranging from 40 to 600 nm, was shown using atomic force microscopy and photon correlation spectroscopy. Rapid uptake (1 h) of Cy5-labeled nanoparticles into NIH 3T3 cells, followed by accumulation over a 24 h period, was visualized using fluorescence microscopy. Nanoparticle-mediated knockdown of endogenous enhanced green fluorescent protein (EGFP) was demonstrated in both H1299 human lung carcinoma cells and murine peritoneal macrophages (77.9% and 89.3% reduction in EGFP fluorescence, respectively). In addition, Western analysis showed approximately 90% reduced expression of BCR/ABL-1 leukemia fusion protein while BCR expression was unaffected in K562 (Ph(+)) cells after transfection using nanoparticles containing siRNA specific to the BCR/ABL-1 junction sequence. Effective in vivo RNA interference was achieved in bronchiole epithelial cells of transgenic EGFP mice after nasal administration of chitosan/siRNA formulations (37% and 43% reduction compared to mismatch and untreated control, respectively). These findings highlight the potential application of this novel chitosan-based system in RNA-mediated therapy of systemic and mucosal disease.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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