Microarray gene expression profiling during the segmentation phase of zebrafish development.
Identifieur interne : 000316 ( Ncbi/Curation ); précédent : 000315; suivant : 000317Microarray gene expression profiling during the segmentation phase of zebrafish development.
Auteurs : Elwood Linney [États-Unis] ; Betsy Dobbs-Mcauliffe ; Hedieh Sajadi ; Renae L. MalekSource :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN messager (analyse), Analyse de profil d'expression de gènes (normes), Analyse de regroupements, Animaux, Contrôle de qualité, Cytochrome P-450 enzyme system (métabolisme), Danio zébré (embryologie), Danio zébré (génétique), Embryon non mammalien (embryologie), Embryon non mammalien (métabolisme), Facteurs temps, Hybridation d'acides nucléiques, Hybridation in situ, Protéines de poisson-zèbre (génétique), Reproductibilité des résultats, Retinoic acid 4-hydroxylase, Régulation de l'expression des gènes au cours du développement, Séquençage par oligonucléotides en batterie (normes).
- MESH :
- analyse : ARN messager.
- embryologie : Danio zébré, Embryon non mammalien.
- génétique : Danio zébré, Protéines de poisson-zèbre.
- métabolisme : Cytochrome P-450 enzyme system, Embryon non mammalien.
- normes : Analyse de profil d'expression de gènes, Séquençage par oligonucléotides en batterie.
- Analyse de regroupements, Animaux, Contrôle de qualité, Facteurs temps, Hybridation d'acides nucléiques, Hybridation in situ, Reproductibilité des résultats, Retinoic acid 4-hydroxylase, Régulation de l'expression des gènes au cours du développement.
English descriptors
- KwdEn :
- Animals, Cluster Analysis, Cytochrome P-450 Enzyme System (metabolism), Embryo, Nonmammalian (embryology), Embryo, Nonmammalian (metabolism), Gene Expression Profiling (standards), Gene Expression Regulation, Developmental, In Situ Hybridization, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis (standards), Quality Control, RNA, Messenger (analysis), Reproducibility of Results, Retinoic Acid 4-Hydroxylase, Time Factors, Zebrafish (embryology), Zebrafish (genetics), Zebrafish Proteins (genetics).
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : Zebrafish Proteins.
- chemical , metabolism : Cytochrome P-450 Enzyme System.
- embryology : Embryo, Nonmammalian, Zebrafish.
- genetics : Zebrafish.
- metabolism : Embryo, Nonmammalian.
- standards : Gene Expression Profiling, Oligonucleotide Array Sequence Analysis.
- Animals, Cluster Analysis, Gene Expression Regulation, Developmental, In Situ Hybridization, Nucleic Acid Hybridization, Quality Control, Reproducibility of Results, Retinoic Acid 4-Hydroxylase, Time Factors.
Abstract
We analyzed 15,512 unique transcripts from wild-type Danio rerio using a long oligonucleotide microarray containing >16,000 65-mers probes. Total RNA was isolated from staged embryos at 2 h intervals over a 24-h period. On average, at any given time point, 27% of the probe set detected corresponding transcripts in embryonic RNA. There were two predominant patterns in the nearly 4000 genes that changed expression in at least one time point during the first 24 hpf. At 12 hpf, we detected 420 up-regulated and 386 down-regulated genes. By 24 hpf, the number of up- and down-regulated genes had increased to 954 and 766, respectively. While the majority of these genes maintained their new level of expression for the duration of the time course, we identified five genes with phasic regulation over the 24-h time course. Two of these genes, germ cell nuclear factor and mesogenin, have been identified as being expressed during gastrulation (5 1/4 to 10 h postfertilization) and subsequently repressed. A cluster containing 36 distinct ribosomal proteins was up-regulated at 12 h, indicating a capability for de novo protein synthesis during and after this stage. Twenty-three muscle-specific genes were up-regulated late during the initial 24 hpf, corresponding to the development and differentiation of the somites.
DOI: 10.1016/j.cca.2004.08.008
PubMed: 15533793
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Malek</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. 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Total RNA was isolated from staged embryos at 2 h intervals over a 24-h period. On average, at any given time point, 27% of the probe set detected corresponding transcripts in embryonic RNA. There were two predominant patterns in the nearly 4000 genes that changed expression in at least one time point during the first 24 hpf. At 12 hpf, we detected 420 up-regulated and 386 down-regulated genes. By 24 hpf, the number of up- and down-regulated genes had increased to 954 and 766, respectively. While the majority of these genes maintained their new level of expression for the duration of the time course, we identified five genes with phasic regulation over the 24-h time course. Two of these genes, germ cell nuclear factor and mesogenin, have been identified as being expressed during gastrulation (5 1/4 to 10 h postfertilization) and subsequently repressed. A cluster containing 36 distinct ribosomal proteins was up-regulated at 12 h, indicating a capability for de novo protein synthesis during and after this stage. Twenty-three muscle-specific genes were up-regulated late during the initial 24 hpf, corresponding to the development and differentiation of the somites.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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