Growth control of mouse mammary epithelial cells by keratin antibody-targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor.
Identifieur interne : 000194 ( Ncbi/Curation ); précédent : 000193; suivant : 000195Growth control of mouse mammary epithelial cells by keratin antibody-targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor.
Auteurs : In-Suh Yuh [Corée du Sud] ; Seung-Youp Lee ; Byong-Ju HongSource :
- Cell biology international [ 1065-6995 ] ; 2003.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (administration et posologie), Cellules épithéliales (métabolisme), Femelle, Glandes mammaires animales (cytologie), Glandes mammaires animales (métabolisme), Kératines (administration et posologie), Kératines (immunologie), Lignée cellulaire, Liposomes (immunologie), Oligonucléotides antisens (administration et posologie), Relation dose-effet des médicaments, Récepteurs ErbB (administration et posologie), Récepteurs ErbB (génétique), Récepteurs ErbB (métabolisme), Souris, Systèmes de délivrance de médicaments ().
- MESH :
- administration et posologie : Anticorps, Kératines, Oligonucléotides antisens, Récepteurs ErbB.
- cytologie : Glandes mammaires animales.
- génétique : Récepteurs ErbB.
- immunologie : Kératines, Liposomes.
- métabolisme : Cellules épithéliales, Glandes mammaires animales, Récepteurs ErbB.
- Animaux, Femelle, Lignée cellulaire, Relation dose-effet des médicaments, Souris, Systèmes de délivrance de médicaments.
English descriptors
- KwdEn :
- Animals, Antibodies (administration & dosage), Cell Line, Dose-Response Relationship, Drug, Drug Delivery Systems (methods), Epithelial Cells (metabolism), ErbB Receptors (administration & dosage), ErbB Receptors (genetics), ErbB Receptors (metabolism), Female, Keratins (administration & dosage), Keratins (immunology), Liposomes (immunology), Mammary Glands, Animal (cytology), Mammary Glands, Animal (metabolism), Mice, Oligonucleotides, Antisense (administration & dosage).
- MESH :
- chemical , administration & dosage : Antibodies, ErbB Receptors, Keratins, Oligonucleotides, Antisense.
- chemical , genetics : ErbB Receptors.
- chemical , immunology : Keratins, Liposomes.
- cytology : Mammary Glands, Animal.
- metabolism : Epithelial Cells, ErbB Receptors, Mammary Glands, Animal.
- methods : Drug Delivery Systems.
- Animals, Cell Line, Dose-Response Relationship, Drug, Female, Mice.
Abstract
NMuMG cells were incubated with 17beta-estradiol (E)+progesterone (P)+epidermal growth factor (EGF), with or without various types of oligomers (21-mers) to the EGF receptor activity domain (amino acid residues 718 to 724). Sense or antisense oligomers were encapsulated in protein A-bearing liposomes. Uncoupled protein A and unencapsulated sense or antisense oligomers were separated from liposomes on a Sepharose 4B column (the encapsulation efficiency of oligomers in liposome-protein A was 0.8%). The addition of various concentrations of EGF to E+P showed an interaction between them during DNA synthesis (P<0.05). Antisense oligomers (1 microM) decreased DNA synthesis induced by E+P+EGF (65.0% inhibition, P<0.05). Sense oligomers also inhibited DNA synthesis induced by E+P+EGF (P<0.05). However, random-sequence oligomers did not inhibit EGF-induced DNA synthesis. We cannot rule out the possibility that sense oligomers match an unknown functional gene mRNA involved in cell growth, which causes their inhibitory effect. Cells were incubated with a keratin monoclonal antibody and then with dilutions of protein A-bound liposomes containing sense or antisense oligomers in the presence of E+P+EGF. Dose dependent inhibition of DNA synthesis was observed. The encapsulated oligomers in protein A-bound liposomes inhibited DNA synthesis at a 100-fold lower concentration than that of unencapsulated oligomers or the oligomer+liposome mixture. The tyrosine kinase activity domain has an important role in EGF regulation of mammary growth. The effect of a cytokeratin-targeted antibody on DNA synthesis in normal mouse mammary epithelial cells was marginal.
DOI: 10.1016/s1065-6995(03)00020-9
PubMed: 12758088
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<affiliation wicri:level="1"><nlm:affiliation>Department of Dairy Science, College of Animal Resources Sciences, Kangwon National University, Hyozadong, Chuncheon, Republic of Korea 200-701. insuhyuh@kangwon.ac.kr</nlm:affiliation>
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<author><name sortKey="Hong, Byong Ju" sort="Hong, Byong Ju" uniqKey="Hong B" first="Byong-Ju" last="Hong">Byong-Ju Hong</name>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Dairy Science, College of Animal Resources Sciences, Kangwon National University, Hyozadong, Chuncheon, Republic of Korea 200-701. insuhyuh@kangwon.ac.kr</nlm:affiliation>
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<term>Dose-Response Relationship, Drug</term>
<term>Drug Delivery Systems (methods)</term>
<term>Epithelial Cells (metabolism)</term>
<term>ErbB Receptors (administration & dosage)</term>
<term>ErbB Receptors (genetics)</term>
<term>ErbB Receptors (metabolism)</term>
<term>Female</term>
<term>Keratins (administration & dosage)</term>
<term>Keratins (immunology)</term>
<term>Liposomes (immunology)</term>
<term>Mammary Glands, Animal (cytology)</term>
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<term>Mice</term>
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<term>Anticorps (administration et posologie)</term>
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<term>Femelle</term>
<term>Glandes mammaires animales (cytologie)</term>
<term>Glandes mammaires animales (métabolisme)</term>
<term>Kératines (administration et posologie)</term>
<term>Kératines (immunologie)</term>
<term>Lignée cellulaire</term>
<term>Liposomes (immunologie)</term>
<term>Oligonucléotides antisens (administration et posologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Récepteurs ErbB (administration et posologie)</term>
<term>Récepteurs ErbB (génétique)</term>
<term>Récepteurs ErbB (métabolisme)</term>
<term>Souris</term>
<term>Systèmes de délivrance de médicaments ()</term>
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<term>ErbB Receptors</term>
<term>Keratins</term>
<term>Oligonucleotides, Antisense</term>
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<term>Oligonucléotides antisens</term>
<term>Récepteurs ErbB</term>
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<term>Liposomes</term>
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<term>ErbB Receptors</term>
<term>Mammary Glands, Animal</term>
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<term>Glandes mammaires animales</term>
<term>Récepteurs ErbB</term>
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<term>Dose-Response Relationship, Drug</term>
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<term>Mice</term>
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<term>Femelle</term>
<term>Lignée cellulaire</term>
<term>Relation dose-effet des médicaments</term>
<term>Souris</term>
<term>Systèmes de délivrance de médicaments</term>
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<front><div type="abstract" xml:lang="en">NMuMG cells were incubated with 17beta-estradiol (E)+progesterone (P)+epidermal growth factor (EGF), with or without various types of oligomers (21-mers) to the EGF receptor activity domain (amino acid residues 718 to 724). Sense or antisense oligomers were encapsulated in protein A-bearing liposomes. Uncoupled protein A and unencapsulated sense or antisense oligomers were separated from liposomes on a Sepharose 4B column (the encapsulation efficiency of oligomers in liposome-protein A was 0.8%). The addition of various concentrations of EGF to E+P showed an interaction between them during DNA synthesis (P<0.05). Antisense oligomers (1 microM) decreased DNA synthesis induced by E+P+EGF (65.0% inhibition, P<0.05). Sense oligomers also inhibited DNA synthesis induced by E+P+EGF (P<0.05). However, random-sequence oligomers did not inhibit EGF-induced DNA synthesis. We cannot rule out the possibility that sense oligomers match an unknown functional gene mRNA involved in cell growth, which causes their inhibitory effect. Cells were incubated with a keratin monoclonal antibody and then with dilutions of protein A-bound liposomes containing sense or antisense oligomers in the presence of E+P+EGF. Dose dependent inhibition of DNA synthesis was observed. The encapsulated oligomers in protein A-bound liposomes inhibited DNA synthesis at a 100-fold lower concentration than that of unencapsulated oligomers or the oligomer+liposome mixture. The tyrosine kinase activity domain has an important role in EGF regulation of mammary growth. The effect of a cytokeratin-targeted antibody on DNA synthesis in normal mouse mammary epithelial cells was marginal.</div>
</front>
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