Bacteriophage P2 and P4 morphogenesis: structure and function of the connector.
Identifieur interne : 002B18 ( Ncbi/Checkpoint ); précédent : 002B17; suivant : 002B19Bacteriophage P2 and P4 morphogenesis: structure and function of the connector.
Auteurs : S. Rishovd [Norvège] ; A. Holzenburg ; B V Johansen ; B H LindqvistSource :
- Virology [ 0042-6822 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
- physiologie : Bactériophage P2, Capside.
- ultrastructure : Assemblage viral, Bactériophage P2, Capside.
English descriptors
- KwdEn :
- MESH :
- physiology : Bacteriophage P2, Capsid.
- ultrastructure : Bacteriophage P2, Capsid.
- Virus Assembly.
Abstract
The connector, the structure located between the bacteriophage capsid and tail, is interesting from several points of view. The connector is in many cases involved in the initiation of the capsid assembly process, functions as a gate for DNA transport in and out of the capsid, and is, as implied by the name, the structure connecting a tail to the capsid. Occupying a position on a 5-fold axis in the capsid and connected to a coaxial 6-fold tail, it mediates a symmetry mismatch between the two. To understand how the connector is capable of all these interactions its structure needs to be worked out. We have focused on the bacteriophage P2/P4 connector, and here we report an image reconstruction based on 2D crystalline layers of connector protein expressed from a plasmid in the absence of other phage proteins. The overall design of the connector complies well with that of other phage connectors, being a toroid structure having a conspicuous central channel. Our data suggests a 12-fold symmetry, i.e., 12 protrusions emerge from the more compact central part of the structure. However, rotational analysis of single particles suggests that there are both 12- and 13-mers present in the crude sample. The connectors used in this image reconstruction work differ from connectors in virions by having retained the amino-terminal 26 amino acids normally cleaved off during the morphogenetic process. We have used different late gene mutants to demonstrate that this processing occurs during DNA packaging, since only mutants in gene P, coding for the large terminase subunit, accumulate uncleaved connector protein. The suggestion that the cleavage might be intimately involved in the DNA packaging process is substantiated by the fact that the fragment cleaved off is highly basic and is homologous to known DNA binding sequences.
DOI: 10.1006/viro.1998.9153
PubMed: 9614863
Affiliations:
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Holzenburg</name></author><author><name sortKey="Johansen, B V" sort="Johansen, B V" uniqKey="Johansen B" first="B V" last="Johansen">B V Johansen</name></author><author><name sortKey="Lindqvist, B H" sort="Lindqvist, B H" uniqKey="Lindqvist B" first="B H" last="Lindqvist">B H Lindqvist</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9614863</idno><idno type="pmid">9614863</idno><idno type="doi">10.1006/viro.1998.9153</idno><idno type="wicri:Area/PubMed/Corpus">002686</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002686</idno><idno type="wicri:Area/PubMed/Curation">002686</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002686</idno><idno type="wicri:Area/PubMed/Checkpoint">002556</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002556</idno><idno type="wicri:Area/Ncbi/Merge">002B18</idno><idno type="wicri:Area/Ncbi/Curation">002B18</idno><idno type="wicri:Area/Ncbi/Checkpoint">002B18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bacteriophage P2 and P4 morphogenesis: structure and function of the connector.</title><author><name sortKey="Rishovd, S" sort="Rishovd, S" uniqKey="Rishovd S" first="S" last="Rishovd">S. Rishovd</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biology, University of Oslo, Norway.</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute of Biology, University of Oslo</wicri:regionArea><wicri:noRegion>University of Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Holzenburg, A" sort="Holzenburg, A" uniqKey="Holzenburg A" first="A" last="Holzenburg">A. Holzenburg</name></author><author><name sortKey="Johansen, B V" sort="Johansen, B V" uniqKey="Johansen B" first="B V" last="Johansen">B V Johansen</name></author><author><name sortKey="Lindqvist, B H" sort="Lindqvist, B H" uniqKey="Lindqvist B" first="B H" last="Lindqvist">B H Lindqvist</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriophage P2 (physiology)</term><term>Bacteriophage P2 (ultrastructure)</term><term>Capsid (physiology)</term><term>Capsid (ultrastructure)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Assemblage viral</term><term>Bactériophage P2 (physiologie)</term><term>Bactériophage P2 (ultrastructure)</term><term>Capside (physiologie)</term><term>Capside (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bactériophage P2</term><term>Capside</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bacteriophage P2</term><term>Capsid</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Bacteriophage P2</term><term>Capsid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Virus Assembly</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Assemblage viral</term><term>Bactériophage P2</term><term>Capside</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The connector, the structure located between the bacteriophage capsid and tail, is interesting from several points of view. The connector is in many cases involved in the initiation of the capsid assembly process, functions as a gate for DNA transport in and out of the capsid, and is, as implied by the name, the structure connecting a tail to the capsid. Occupying a position on a 5-fold axis in the capsid and connected to a coaxial 6-fold tail, it mediates a symmetry mismatch between the two. To understand how the connector is capable of all these interactions its structure needs to be worked out. We have focused on the bacteriophage P2/P4 connector, and here we report an image reconstruction based on 2D crystalline layers of connector protein expressed from a plasmid in the absence of other phage proteins. The overall design of the connector complies well with that of other phage connectors, being a toroid structure having a conspicuous central channel. Our data suggests a 12-fold symmetry, i.e., 12 protrusions emerge from the more compact central part of the structure. However, rotational analysis of single particles suggests that there are both 12- and 13-mers present in the crude sample. The connectors used in this image reconstruction work differ from connectors in virions by having retained the amino-terminal 26 amino acids normally cleaved off during the morphogenetic process. We have used different late gene mutants to demonstrate that this processing occurs during DNA packaging, since only mutants in gene P, coding for the large terminase subunit, accumulate uncleaved connector protein. The suggestion that the cleavage might be intimately involved in the DNA packaging process is substantiated by the fact that the fragment cleaved off is highly basic and is homologous to known DNA binding sequences.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><noCountry><name sortKey="Holzenburg, A" sort="Holzenburg, A" uniqKey="Holzenburg A" first="A" last="Holzenburg">A. Holzenburg</name><name sortKey="Johansen, B V" sort="Johansen, B V" uniqKey="Johansen B" first="B V" last="Johansen">B V Johansen</name><name sortKey="Lindqvist, B H" sort="Lindqvist, B H" uniqKey="Lindqvist B" first="B H" last="Lindqvist">B H Lindqvist</name></noCountry><country name="Norvège"><noRegion><name sortKey="Rishovd, S" sort="Rishovd, S" uniqKey="Rishovd S" first="S" last="Rishovd">S. Rishovd</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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