DNA interference by a mesophilic Argonaute protein, CbcAgo
Identifieur interne : 002533 ( Ncbi/Checkpoint ); précédent : 002532; suivant : 002534DNA interference by a mesophilic Argonaute protein, CbcAgo
Auteurs : Nieves García-Quintans [Espagne] ; Laurie Bowden [Espagne] ; José Berenguer [Espagne] ; Mario Mencía [Espagne]Source :
- F1000Research [ 2046-1402 ] ; 2020.
Abstract
Url:
DOI: 10.12688/f1000research.18445.2
PubMed: 32055395
PubMed Central: 6961421
Affiliations:
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<author><name sortKey="Garcia Quintans, Nieves" sort="Garcia Quintans, Nieves" uniqKey="Garcia Quintans N" first="Nieves" last="García-Quintans">Nieves García-Quintans</name>
<affiliation wicri:level="2"><nlm:aff id="a1">Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049, Spain</nlm:aff>
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<author><name sortKey="Bowden, Laurie" sort="Bowden, Laurie" uniqKey="Bowden L" first="Laurie" last="Bowden">Laurie Bowden</name>
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<author><name sortKey="Berenguer, Jose" sort="Berenguer, Jose" uniqKey="Berenguer J" first="José" last="Berenguer">José Berenguer</name>
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<author><name sortKey="Mencia, Mario" sort="Mencia, Mario" uniqKey="Mencia M" first="Mario" last="Mencía">Mario Mencía</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">DNA interference by a mesophilic Argonaute protein, CbcAgo</title>
<author><name sortKey="Garcia Quintans, Nieves" sort="Garcia Quintans, Nieves" uniqKey="Garcia Quintans N" first="Nieves" last="García-Quintans">Nieves García-Quintans</name>
<affiliation wicri:level="2"><nlm:aff id="a1">Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bowden, Laurie" sort="Bowden, Laurie" uniqKey="Bowden L" first="Laurie" last="Bowden">Laurie Bowden</name>
<affiliation wicri:level="2"><nlm:aff id="a1">Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Berenguer, Jose" sort="Berenguer, Jose" uniqKey="Berenguer J" first="José" last="Berenguer">José Berenguer</name>
<affiliation wicri:level="2"><nlm:aff id="a1">Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mencia, Mario" sort="Mencia, Mario" uniqKey="Mencia M" first="Mario" last="Mencía">Mario Mencía</name>
<affiliation wicri:level="2"><nlm:aff id="a1">Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Madrid, 28049</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
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</analytic>
<series><title level="j">F1000Research</title>
<idno type="eISSN">2046-1402</idno>
<imprint><date when="2020">2020</date>
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<front><div type="abstract" xml:lang="en"><p><bold>Background</bold>
: The search for putative enzymes that can facilitate gene editing has recently focused its attention on Argonaute proteins from prokaryotes (pAgos). Though they are structural homologues of human Argonaute protein, which uses RNA guides to interfere with RNA targets, pAgos use ssDNA guides to identify and, in many cases, cut a complementary DNA target. Thermophilic pAgos from
<italic>Thermus thermophilus</italic>
,
<italic>Pyrococcus furiosus</italic>
and
<italic>Methanocaldococcus jasmanii</italic>
have been identified and thoroughly studied, but their thermoactivity makes them of little use in mesophilic systems such as mammalian cells.</p>
<p><bold>Methods</bold>
: Here we search for and identify CbcAgo, a prokaryotic Argonaute protein from a mesophilic bacterium, and characterize
<italic>in vitro</italic>
its DNA interference activity.</p>
<p><bold>Results</bold>
: CbcAgo efficiently uses 5’P-ssDNA guides as small as 11-mers to cut ssDNA targets, requires divalent cations (preferentially, Mn
<sup>2+</sup>
) and has a maximum activity between 37 and 42 °C, remaining active up to 55 °C. Nicking activity on supercoiled dsDNA was shown. However, no efficient double-strand breaking activity could be demonstrated.</p>
<p><bold>Conclusions</bold>
: CbcAgo can use gDNA guides as small as 11 nucleotides long to cut complementary ssDNA targets at 37ºC, making it a promising starting point for the development of new gene editing tools for mammalian cells.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Meister, G" uniqKey="Meister G">G Meister</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Makarova, K" uniqKey="Makarova K">K Makarova</name>
</author>
<author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Jore, Mm" uniqKey="Jore M">MM Jore</name>
</author>
<author><name sortKey="Westra, Er" uniqKey="Westra E">ER Westra</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Hegge, Jw" uniqKey="Hegge J">JW Hegge</name>
</author>
<author><name sortKey="Hinojo, I" uniqKey="Hinojo I">I Hinojo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zander, A" uniqKey="Zander A">A Zander</name>
</author>
<author><name sortKey="Willkomm, S" uniqKey="Willkomm S">S Willkomm</name>
</author>
<author><name sortKey="Ofer, S" uniqKey="Ofer S">S Ofer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yuan, Yr" uniqKey="Yuan Y">YR Yuan</name>
</author>
<author><name sortKey="Pei, Y" uniqKey="Pei Y">Y Pei</name>
</author>
<author><name sortKey="Ma, Jb" uniqKey="Ma J">JB Ma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sunghyeok, Y" uniqKey="Sunghyeok Y">Y Sunghyeok</name>
</author>
<author><name sortKey="Taegeun, B" uniqKey="Taegeun B">B Taegeun</name>
</author>
<author><name sortKey="Kyoungmi, K" uniqKey="Kyoungmi K">K Kyoungmi,</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olovnikov, I" uniqKey="Olovnikov I">I Olovnikov</name>
</author>
<author><name sortKey="Chan, K" uniqKey="Chan K">K Chan</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sheng, G" uniqKey="Sheng G">G Sheng</name>
</author>
<author><name sortKey="Zhao, H" uniqKey="Zhao H">H Zhao</name>
</author>
<author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Szczepaniak, M" uniqKey="Szczepaniak M">M Szczepaniak</name>
</author>
<author><name sortKey="Sheng, G" uniqKey="Sheng G">G Sheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hegge, Jw" uniqKey="Hegge J">JW Hegge</name>
</author>
<author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Van Der Oost, J" uniqKey="Van Der Oost J">J van der Oost</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gao, F" uniqKey="Gao F">F Gao</name>
</author>
<author><name sortKey="Shen, Xz" uniqKey="Shen X">XZ Shen</name>
</author>
<author><name sortKey="Jiang, F" uniqKey="Jiang F">F Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, Sh" uniqKey="Lee S">SH Lee</name>
</author>
<author><name sortKey="Turchiano, G" uniqKey="Turchiano G">G Turchiano</name>
</author>
<author><name sortKey="Ata, H" uniqKey="Ata H">H Ata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hegge, Jw" uniqKey="Hegge J">JW Hegge</name>
</author>
<author><name sortKey="Swarts, Dc" uniqKey="Swarts D">DC Swarts</name>
</author>
<author><name sortKey="Chandradoss, Sd" uniqKey="Chandradoss S">SD Chandradoss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuzmenko, A" uniqKey="Kuzmenko A">A Kuzmenko</name>
</author>
<author><name sortKey="Yudin, D" uniqKey="Yudin D">D Yudin</name>
</author>
<author><name sortKey="Ryazansky, S" uniqKey="Ryazansky S">S Ryazansky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name>
</author>
<author><name sortKey="Sheng, G" uniqKey="Sheng G">G Sheng</name>
</author>
<author><name sortKey="Juranek, S" uniqKey="Juranek S">S Juranek</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Berenguer, J" uniqKey="Berenguer J">J Berenguer</name>
</author>
</analytic>
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