Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
Identifieur interne : 002457 ( Ncbi/Checkpoint ); précédent : 002456; suivant : 002458Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
Auteurs :Source :
- MERS Coronavirus ; 2019.
Abstract
Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species.
Url:
DOI: 10.1007/978-1-0716-0211-9_10
PubMed: 31883092
PubMed Central: 7123069
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000184
- to stream Pmc, to step Curation: 000184
- to stream Pmc, to step Checkpoint: 000301
- to stream PubMed, to step Corpus: 000308
- to stream PubMed, to step Curation: 000308
- to stream PubMed, to step Checkpoint: 000197
- to stream Ncbi, to step Merge: 002457
- to stream Ncbi, to step Curation: 002457
Links to Exploration step
PMC:7123069Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera</title>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">31883092</idno>
<idno type="pmc">7123069</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123069</idno>
<idno type="RBID">PMC:7123069</idno>
<idno type="doi">10.1007/978-1-0716-0211-9_10</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000184</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000184</idno>
<idno type="wicri:Area/Pmc/Curation">000184</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000184</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000301</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000301</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:31883092</idno>
<idno type="wicri:Area/PubMed/Corpus">000308</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000308</idno>
<idno type="wicri:Area/PubMed/Curation">000308</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000308</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000197</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000197</idno>
<idno type="wicri:Area/Ncbi/Merge">002457</idno>
<idno type="wicri:Area/Ncbi/Curation">002457</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002457</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera</title>
</analytic>
<series><title level="j">MERS Coronavirus</title>
<imprint><date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rous, P" uniqKey="Rous P">P Rous</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carnell, Gw" uniqKey="Carnell G">GW Carnell</name>
</author>
<author><name sortKey="Ferrara, F" uniqKey="Ferrara F">F Ferrara</name>
</author>
<author><name sortKey="Grehan, K" uniqKey="Grehan K">K Grehan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ferrara, F" uniqKey="Ferrara F">F Ferrara</name>
</author>
<author><name sortKey="Temperton, N" uniqKey="Temperton N">N Temperton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Urbanowicz, Ra" uniqKey="Urbanowicz R">RA Urbanowicz</name>
</author>
<author><name sortKey="Mcclure, Cp" uniqKey="Mcclure C">CP McClure</name>
</author>
<author><name sortKey="King, B" uniqKey="King B">B King</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chin, Awh" uniqKey="Chin A">AWH Chin</name>
</author>
<author><name sortKey="Perera, Rapm" uniqKey="Perera R">RAPM Perera</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grehan, K" uniqKey="Grehan K">K Grehan</name>
</author>
<author><name sortKey="Ferrara, F" uniqKey="Ferrara F">F Ferrara</name>
</author>
<author><name sortKey="Temperton, N" uniqKey="Temperton N">N Temperton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cronin, J" uniqKey="Cronin J">J Cronin</name>
</author>
<author><name sortKey="Zhang, X Y" uniqKey="Zhang X">X-Y Zhang</name>
</author>
<author><name sortKey="Reiser, J" uniqKey="Reiser J">J Reiser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schnierle, Bs" uniqKey="Schnierle B">BS Schnierle</name>
</author>
<author><name sortKey="Stitz, J" uniqKey="Stitz J">J Stitz</name>
</author>
<author><name sortKey="Bosch, V" uniqKey="Bosch V">V Bosch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zufferey, R" uniqKey="Zufferey R">R Zufferey</name>
</author>
<author><name sortKey="Dull, T" uniqKey="Dull T">T Dull</name>
</author>
<author><name sortKey="Mandel, Rj" uniqKey="Mandel R">RJ Mandel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Unseld, M" uniqKey="Unseld M">M Unseld</name>
</author>
<author><name sortKey="Marienfeld, Jr" uniqKey="Marienfeld J">JR Marienfeld</name>
</author>
<author><name sortKey="Brandt, P" uniqKey="Brandt P">P Brandt</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list></list>
<tree></tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002457 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 002457 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= PMC:7123069 |texte= Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:31883092" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |