Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
Identifieur interne : 002396 ( Ncbi/Checkpoint ); précédent : 002395; suivant : 002397Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
Auteurs : Jordi Rodon [Espagne] ; Nisreen M. A. Okba [Pays-Bas] ; Nigeer Te [Espagne] ; Brenda Van Dieren [Pays-Bas] ; Berend-Jan Bosch [Pays-Bas] ; Albert Bensaid [Espagne] ; Joaquim Segalés [Espagne] ; Bart L. Haagmans [Pays-Bas] ; Júlia Vergara-Alert [Espagne]Source :
- Emerging Microbes & Infections [ 2222-1751 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps neutralisants (immunologie), Camélidés du Nouveau Monde, Chameaux (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Glycoprotéine de spicule des coronavirus (administration et posologie), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (immunologie), Humains, Infections à coronavirus (), Infections à coronavirus (virologie), Vaccination, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Zoonoses (), Zoonoses (immunologie), Zoonoses (transmission), Zoonoses (virologie).
- MESH :
- administration et posologie : Glycoprotéine de spicule des coronavirus, Vaccins antiviraux.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Vaccins antiviraux.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Vaccins antiviraux, Zoonoses.
- virologie : Chameaux, Infections à coronavirus, Zoonoses.
- Animaux, Camélidés du Nouveau Monde, Humains, Infections à coronavirus, Vaccination, Zoonoses.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Camelids, New World, Camelus (virology), Coronavirus Infections (prevention & control), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Spike Glycoprotein, Coronavirus (administration & dosage), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Vaccination, Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology), Zoonoses (immunology), Zoonoses (prevention & control), Zoonoses (transmission), Zoonoses (virology).
- MESH :
- chemical , administration & dosage : Spike Glycoprotein, Coronavirus, Viral Vaccines.
- chemical , genetics : Spike Glycoprotein, Coronavirus, Viral Vaccines.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus, Zoonoses.
- prevention & control : Coronavirus Infections, Zoonoses.
- transmission : Zoonoses.
- virology : Camelus, Coronavirus Infections, Zoonoses.
- Animals, Camelids, New World, Humans, Vaccination.
Abstract
The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naïve animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naïve animals 4–5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naïve animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans.
Url:
DOI: 10.1080/22221751.2019.1685912
PubMed: 31711379
PubMed Central: 6853226
Affiliations:
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PMC:6853226Le document en format XML
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naïve animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naïve animals 4–5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naïve animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans.</p>
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