The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator.
Identifieur interne : 001978 ( Ncbi/Checkpoint ); précédent : 001977; suivant : 001979The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator.
Auteurs : N. Webster [France] ; J R Jin ; S. Green ; M. Hollis ; P. ChambonSource :
- Cell [ 0092-8674 ] ; 1988.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), ADN recombiné, Animaux, Cellules HeLa (métabolisme), Facteurs de transcription, Globines (génétique), Humains, Lapins, Oestrogènes (pharmacologie), Plasmides, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ADN, Protéines fongiques (génétique), Récepteurs des oestrogènes (génétique), Régions promotrices (génétique), Simplexvirus (enzymologie), Simplexvirus (génétique), Séquences d'acides nucléiques régulatrices, Thymidine (génétique), Transcription génétique, Transfection, Virus simien 40 (génétique), Éléments activateurs (génétique).
- MESH :
- enzymologie : Simplexvirus.
- génétique : ADN fongique, Globines, Protéines fongiques, Récepteurs des oestrogènes, Simplexvirus, Thymidine, Virus simien 40.
- métabolisme : Cellules HeLa.
- pharmacologie : Oestrogènes.
- ADN recombiné, Animaux, Facteurs de transcription, Humains, Lapins, Plasmides, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ADN, Régions promotrices (génétique), Séquences d'acides nucléiques régulatrices, Transcription génétique, Transfection, Éléments activateurs (génétique).
English descriptors
- KwdEn :
- Animals, DNA, Fungal (genetics), DNA, Recombinant, DNA-Binding Proteins, Enhancer Elements, Genetic, Estrogens (pharmacology), Fungal Proteins (genetics), Globins (genetics), HeLa Cells (metabolism), Humans, Plasmids, Promoter Regions, Genetic, Rabbits, Receptors, Estrogen (genetics), Regulatory Sequences, Nucleic Acid, Saccharomyces cerevisiae Proteins, Simian virus 40 (genetics), Simplexvirus (enzymology), Simplexvirus (genetics), Thymidine (genetics), Transcription Factors, Transcription, Genetic, Transfection.
- MESH :
- chemical , genetics : DNA, Fungal, Fungal Proteins, Globins, Receptors, Estrogen, Thymidine.
- chemical , pharmacology : Estrogens.
- enzymology : Simplexvirus.
- genetics : Simian virus 40, Simplexvirus.
- metabolism : HeLa Cells.
- Animals, DNA, Recombinant, DNA-Binding Proteins, Enhancer Elements, Genetic, Humans, Plasmids, Promoter Regions, Genetic, Rabbits, Regulatory Sequences, Nucleic Acid, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic, Transfection.
Abstract
The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating "acidic" regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.
DOI: 10.1016/0092-8674(88)90505-3
PubMed: 2830022
Affiliations:
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pubmed:2830022Le document en format XML
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<author><name sortKey="Webster, N" sort="Webster, N" uniqKey="Webster N" first="N" last="Webster">N. Webster</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.</nlm:affiliation>
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<author><name sortKey="Jin, J R" sort="Jin, J R" uniqKey="Jin J" first="J R" last="Jin">J R Jin</name>
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<author><name sortKey="Green, S" sort="Green, S" uniqKey="Green S" first="S" last="Green">S. Green</name>
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<author><name sortKey="Hollis, M" sort="Hollis, M" uniqKey="Hollis M" first="M" last="Hollis">M. Hollis</name>
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<term>DNA, Fungal (genetics)</term>
<term>DNA, Recombinant</term>
<term>DNA-Binding Proteins</term>
<term>Enhancer Elements, Genetic</term>
<term>Estrogens (pharmacology)</term>
<term>Fungal Proteins (genetics)</term>
<term>Globins (genetics)</term>
<term>HeLa Cells (metabolism)</term>
<term>Humans</term>
<term>Plasmids</term>
<term>Promoter Regions, Genetic</term>
<term>Rabbits</term>
<term>Receptors, Estrogen (genetics)</term>
<term>Regulatory Sequences, Nucleic Acid</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Simian virus 40 (genetics)</term>
<term>Simplexvirus (enzymology)</term>
<term>Simplexvirus (genetics)</term>
<term>Thymidine (genetics)</term>
<term>Transcription Factors</term>
<term>Transcription, Genetic</term>
<term>Transfection</term>
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<term>ADN recombiné</term>
<term>Animaux</term>
<term>Cellules HeLa (métabolisme)</term>
<term>Facteurs de transcription</term>
<term>Globines (génétique)</term>
<term>Humains</term>
<term>Lapins</term>
<term>Oestrogènes (pharmacologie)</term>
<term>Plasmides</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ADN</term>
<term>Protéines fongiques (génétique)</term>
<term>Récepteurs des oestrogènes (génétique)</term>
<term>Régions promotrices (génétique)</term>
<term>Simplexvirus (enzymologie)</term>
<term>Simplexvirus (génétique)</term>
<term>Séquences d'acides nucléiques régulatrices</term>
<term>Thymidine (génétique)</term>
<term>Transcription génétique</term>
<term>Transfection</term>
<term>Virus simien 40 (génétique)</term>
<term>Éléments activateurs (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term>
<term>Fungal Proteins</term>
<term>Globins</term>
<term>Receptors, Estrogen</term>
<term>Thymidine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Estrogens</term>
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<term>Simplexvirus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term>
<term>Globines</term>
<term>Protéines fongiques</term>
<term>Récepteurs des oestrogènes</term>
<term>Simplexvirus</term>
<term>Thymidine</term>
<term>Virus simien 40</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HeLa Cells</term>
</keywords>
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</keywords>
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</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>DNA, Recombinant</term>
<term>DNA-Binding Proteins</term>
<term>Enhancer Elements, Genetic</term>
<term>Humans</term>
<term>Plasmids</term>
<term>Promoter Regions, Genetic</term>
<term>Rabbits</term>
<term>Regulatory Sequences, Nucleic Acid</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
<term>Transcription, Genetic</term>
<term>Transfection</term>
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<term>Animaux</term>
<term>Facteurs de transcription</term>
<term>Humains</term>
<term>Lapins</term>
<term>Plasmides</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ADN</term>
<term>Régions promotrices (génétique)</term>
<term>Séquences d'acides nucléiques régulatrices</term>
<term>Transcription génétique</term>
<term>Transfection</term>
<term>Éléments activateurs (génétique)</term>
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<front><div type="abstract" xml:lang="en">The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating "acidic" regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.</div>
</front>
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