Nucleic acid separations using superficially porous silica particles
Identifieur interne : 001518 ( Ncbi/Checkpoint ); précédent : 001517; suivant : 001519Nucleic acid separations using superficially porous silica particles
Auteurs : Elizabeth D. Close [Royaume-Uni] ; Alison O. Nwokeoji [Royaume-Uni] ; Dafydd Milton [Royaume-Uni] ; Ken Cook [Royaume-Uni] ; Darsha M. Hindocha [Royaume-Uni] ; Elliot C. Hook [Royaume-Uni] ; Helen Wood [Royaume-Uni] ; Mark J. Dickman [Royaume-Uni]Source :
- Journal of Chromatography. a [ 0021-9673 ] ; 2016.
Abstract
Superficially porous silica particles enable high resolution separation of nucleic acids. The pore size of the C18 superficially porous silica particles significantly effects resolution. Optimum separations of small oligonucleotides obtained with 80 Å pore sizes. Optimum resolution of oligonucleotides (>19 mers) was observed with pore sizes of 150 Å. Improved resolution of larger dsDNA/RNA molecules was achieved with pore sizes of 400 Å
Url:
DOI: 10.1016/j.chroma.2016.02.057
PubMed: 26948761
PubMed Central: 4801196
Affiliations:
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Dickman</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD</wicri:regionArea><wicri:noRegion>Sheffield S1 3JD</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26948761</idno><idno type="pmc">4801196</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801196</idno><idno type="RBID">PMC:4801196</idno><idno type="doi">10.1016/j.chroma.2016.02.057</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000D90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D90</idno><idno type="wicri:Area/Pmc/Curation">000D90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D90</idno><idno type="wicri:Area/Pmc/Checkpoint">000A53</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A53</idno><idno type="wicri:Area/Ncbi/Merge">001518</idno><idno type="wicri:Area/Ncbi/Curation">001518</idno><idno type="wicri:Area/Ncbi/Checkpoint">001518</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nucleic acid separations using superficially porous silica particles</title><author><name sortKey="Close, Elizabeth D" sort="Close, Elizabeth D" uniqKey="Close E" first="Elizabeth D." last="Close">Elizabeth D. Close</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD</wicri:regionArea><wicri:noRegion>Sheffield S1 3JD</wicri:noRegion></affiliation></author><author><name sortKey="Nwokeoji, Alison O" sort="Nwokeoji, Alison O" uniqKey="Nwokeoji A" first="Alison O." last="Nwokeoji">Alison O. Nwokeoji</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD</wicri:regionArea><wicri:noRegion>Sheffield S1 3JD</wicri:noRegion></affiliation></author><author><name sortKey="Milton, Dafydd" sort="Milton, Dafydd" uniqKey="Milton D" first="Dafydd" last="Milton">Dafydd Milton</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Thermo Fisher Scientific, Stafford House, Boundary Way, Hemel Hempstead HP2 7GE, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Thermo Fisher Scientific, Stafford House, Boundary Way, Hemel Hempstead HP2 7GE</wicri:regionArea><wicri:noRegion>Hemel Hempstead HP2 7GE</wicri:noRegion></affiliation></author><author><name sortKey="Cook, Ken" sort="Cook, Ken" uniqKey="Cook K" first="Ken" last="Cook">Ken Cook</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Thermo Fisher Scientific, Stafford House, Boundary Way, Hemel Hempstead HP2 7GE, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Thermo Fisher Scientific, Stafford House, Boundary Way, Hemel Hempstead HP2 7GE</wicri:regionArea><wicri:noRegion>Hemel Hempstead HP2 7GE</wicri:noRegion></affiliation></author><author><name sortKey="Hindocha, Darsha M" sort="Hindocha, Darsha M" uniqKey="Hindocha D" first="Darsha M." last="Hindocha">Darsha M. Hindocha</name><affiliation wicri:level="1"><nlm:aff id="aff0015">GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY</wicri:regionArea><wicri:noRegion>Hertfordshire SG1 2NY</wicri:noRegion></affiliation></author><author><name sortKey="Hook, Elliot C" sort="Hook, Elliot C" uniqKey="Hook E" first="Elliot C." last="Hook">Elliot C. Hook</name><affiliation wicri:level="1"><nlm:aff id="aff0015">GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY</wicri:regionArea><wicri:noRegion>Hertfordshire SG1 2NY</wicri:noRegion></affiliation></author><author><name sortKey="Wood, Helen" sort="Wood, Helen" uniqKey="Wood H" first="Helen" last="Wood">Helen Wood</name><affiliation wicri:level="1"><nlm:aff id="aff0015">GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY</wicri:regionArea><wicri:noRegion>Hertfordshire SG1 2NY</wicri:noRegion></affiliation></author><author><name sortKey="Dickman, Mark J" sort="Dickman, Mark J" uniqKey="Dickman M" first="Mark J." last="Dickman">Mark J. Dickman</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Chemical and Biological Engineering, ChELSI Institute, University of Sheffield, Mappin Street, Sheffield S1 3JD</wicri:regionArea><wicri:noRegion>Sheffield S1 3JD</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Chromatography. a</title><idno type="ISSN">0021-9673</idno><idno type="eISSN">1873-3778</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple"><list-item id="lsti0005"><label>•</label><p>Superficially porous silica particles enable high resolution separation of nucleic acids.</p></list-item><list-item id="lsti0010"><label>•</label><p>The pore size of the C<sub>18</sub> superficially porous silica particles significantly effects resolution.</p></list-item><list-item id="lsti0015"><label>•</label><p>Optimum separations of small oligonucleotides obtained with 80 Å pore sizes.</p></list-item><list-item id="lsti0020"><label>•</label><p>Optimum resolution of oligonucleotides (>19 mers) was observed with pore sizes of 150 Å.</p></list-item><list-item id="lsti0025"><label>•</label><p>Improved resolution of larger dsDNA/RNA molecules was achieved with pore sizes of 400 Å</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gjerde, D T" uniqKey="Gjerde D">D.T. 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Fountain</name></author><author><name sortKey="Budman, Y" uniqKey="Budman Y">Y. Budman</name></author><author><name sortKey="Holyoke, J L" uniqKey="Holyoke J">J.L. Holyoke</name></author><author><name sortKey="Davoudi, H" uniqKey="Davoudi H">H. Davoudi</name></author><author><name sortKey="Gebler, J C" uniqKey="Gebler J">J.C. Gebler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fountain, K J" uniqKey="Fountain K">K.J. Fountain</name></author><author><name sortKey="Gilar, M" uniqKey="Gilar M">M. Gilar</name></author><author><name sortKey="Gebler, J C" uniqKey="Gebler J">J.C. Gebler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nikcevic, I" uniqKey="Nikcevic I">I. Nikcevic</name></author><author><name sortKey="Wyrzykiewicz, T K" uniqKey="Wyrzykiewicz T">T.K. Wyrzykiewicz</name></author><author><name sortKey="Limbach, P A" uniqKey="Limbach P">P.A. Limbach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Erb, R" uniqKey="Erb R">R. Erb</name></author><author><name sortKey="Leithner, K" uniqKey="Leithner K">K. Leithner</name></author><author><name sortKey="Bernkop Schnurch, A" uniqKey="Bernkop Schnurch A">A. Bernkop-Schnürch</name></author><author><name sortKey="Oberacher, H" uniqKey="Oberacher H">H. Oberacher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stec, W J" uniqKey="Stec W">W.J. Stec</name></author><author><name sortKey="Zon, G" uniqKey="Zon G">G. Zon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slim, G" uniqKey="Slim G">G. Slim</name></author><author><name sortKey="Gait, M J" uniqKey="Gait M">M.J. Gait</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frederiksen, J K" uniqKey="Frederiksen J">J.K. Frederiksen</name></author><author><name sortKey="Piccirilli, J A" uniqKey="Piccirilli J">J.A. Piccirilli</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Close, Elizabeth D" sort="Close, Elizabeth D" uniqKey="Close E" first="Elizabeth D." last="Close">Elizabeth D. Close</name></noRegion><name sortKey="Cook, Ken" sort="Cook, Ken" uniqKey="Cook K" first="Ken" last="Cook">Ken Cook</name><name sortKey="Dickman, Mark J" sort="Dickman, Mark J" uniqKey="Dickman M" first="Mark J." last="Dickman">Mark J. Dickman</name><name sortKey="Hindocha, Darsha M" sort="Hindocha, Darsha M" uniqKey="Hindocha D" first="Darsha M." last="Hindocha">Darsha M. Hindocha</name><name sortKey="Hook, Elliot C" sort="Hook, Elliot C" uniqKey="Hook E" first="Elliot C." last="Hook">Elliot C. Hook</name><name sortKey="Milton, Dafydd" sort="Milton, Dafydd" uniqKey="Milton D" first="Dafydd" last="Milton">Dafydd Milton</name><name sortKey="Nwokeoji, Alison O" sort="Nwokeoji, Alison O" uniqKey="Nwokeoji A" first="Alison O." last="Nwokeoji">Alison O. Nwokeoji</name><name sortKey="Wood, Helen" sort="Wood, Helen" uniqKey="Wood H" first="Helen" last="Wood">Helen Wood</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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