Antisense oligodeoxyribonucleotides inhibit the expression of the gene for hepatitis B virus surface antigen.
Identifieur interne : 000876 ( Ncbi/Checkpoint ); précédent : 000875; suivant : 000877Antisense oligodeoxyribonucleotides inhibit the expression of the gene for hepatitis B virus surface antigen.
Auteurs : G. Goodarzi ; S C Gross ; A. Tewari ; K. WatabeSource :
- The Journal of general virology [ 0022-1317 ] ; 1990.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Antigènes de surface du virus de l'hépatite B (génétique), Carcinome hépatocellulaire, Cinétique, Données de séquences moléculaires, Gènes viraux (), Humains, Hépatite B (génétique), Hépatite B (immunologie), Lignée cellulaire, Oligonucléotides antisens (pharmacologie), Protéines virales structurales (génétique), Séquence nucléotidique, Tumeurs du foie.
- MESH :
- génétique : ARN messager, Antigènes de surface du virus de l'hépatite B, Hépatite B, Protéines virales structurales.
- immunologie : Hépatite B.
- pharmacologie : Oligonucléotides antisens.
- Carcinome hépatocellulaire, Cinétique, Données de séquences moléculaires, Gènes viraux, Humains, Lignée cellulaire, Séquence nucléotidique, Tumeurs du foie.
English descriptors
- KwdEn :
- Base Sequence, Carcinoma, Hepatocellular, Cell Line, Genes, Viral (drug effects), Hepatitis B (genetics), Hepatitis B (immunology), Hepatitis B Surface Antigens (genetics), Humans, Kinetics, Liver Neoplasms, Molecular Sequence Data, Oligonucleotides, Antisense (pharmacology), RNA, Messenger (genetics), Viral Structural Proteins (genetics).
- MESH :
- chemical , genetics : Hepatitis B Surface Antigens, RNA, Messenger, Viral Structural Proteins.
- drug effects : Genes, Viral.
- genetics : Hepatitis B.
- immunology : Hepatitis B.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Base Sequence, Carcinoma, Hepatocellular, Cell Line, Humans, Kinetics, Liver Neoplasms, Molecular Sequence Data.
Abstract
The effect of a series of antisense oligodeoxyribonucleotide [oligo(dN)] on the expression of the surface antigen (HBsAg) gene of human hepatitis B virus (HBV) was examined using hepatocellular carcinoma cells that contain integrated HBV genomes. Of a number of antisense oligo(dN)s tested, synthetic 15-mers directed at the cap site of mRNA and regions of the translational initiation site of the HBsAg gene were found to be highly effective and inhibited viral gene expression by as much as 96%. The inhibition was specific to the HBsAg gene and appeared to be at the level of translation. These results suggest a therapeutic potential for antisense oligo(dN) in the treatment of patients who are chronically infected with HBV.
DOI: 10.1099/0022-1317-71-12-3021
PubMed: 2177093
Affiliations:
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pubmed:2177093Le document en format XML
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Goodarzi</name><affiliation><nlm:affiliation>Department of Medical Microbiology/Immunology, Southern Illinois University School of Medicine, Springfield 62708.</nlm:affiliation><wicri:noCountry code="subField">Springfield 62708</wicri:noCountry></affiliation></author><author><name sortKey="Gross, S C" sort="Gross, S C" uniqKey="Gross S" first="S C" last="Gross">S C Gross</name></author><author><name sortKey="Tewari, A" sort="Tewari, A" uniqKey="Tewari A" first="A" last="Tewari">A. Tewari</name></author><author><name sortKey="Watabe, K" sort="Watabe, K" uniqKey="Watabe K" first="K" last="Watabe">K. Watabe</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Carcinoma, Hepatocellular</term><term>Cell Line</term><term>Genes, Viral (drug effects)</term><term>Hepatitis B (genetics)</term><term>Hepatitis B (immunology)</term><term>Hepatitis B Surface Antigens (genetics)</term><term>Humans</term><term>Kinetics</term><term>Liver Neoplasms</term><term>Molecular Sequence Data</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>RNA, Messenger (genetics)</term><term>Viral Structural Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Antigènes de surface du virus de l'hépatite B (génétique)</term><term>Carcinome hépatocellulaire</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Gènes viraux ()</term><term>Humains</term><term>Hépatite B (génétique)</term><term>Hépatite B (immunologie)</term><term>Lignée cellulaire</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Protéines virales structurales (génétique)</term><term>Séquence nucléotidique</term><term>Tumeurs du foie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hepatitis B Surface Antigens</term><term>RNA, Messenger</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Genes, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepatitis B</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Antigènes de surface du virus de l'hépatite B</term><term>Hépatite B</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Hépatite B</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hepatitis B</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Carcinoma, Hepatocellular</term><term>Cell Line</term><term>Humans</term><term>Kinetics</term><term>Liver Neoplasms</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Carcinome hépatocellulaire</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Gènes viraux</term><term>Humains</term><term>Lignée cellulaire</term><term>Séquence nucléotidique</term><term>Tumeurs du foie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of a series of antisense oligodeoxyribonucleotide [oligo(dN)] on the expression of the surface antigen (HBsAg) gene of human hepatitis B virus (HBV) was examined using hepatocellular carcinoma cells that contain integrated HBV genomes. Of a number of antisense oligo(dN)s tested, synthetic 15-mers directed at the cap site of mRNA and regions of the translational initiation site of the HBsAg gene were found to be highly effective and inhibited viral gene expression by as much as 96%. The inhibition was specific to the HBsAg gene and appeared to be at the level of translation. These results suggest a therapeutic potential for antisense oligo(dN) in the treatment of patients who are chronically infected with HBV.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Goodarzi, G" sort="Goodarzi, G" uniqKey="Goodarzi G" first="G" last="Goodarzi">G. Goodarzi</name><name sortKey="Gross, S C" sort="Gross, S C" uniqKey="Gross S" first="S C" last="Gross">S C Gross</name><name sortKey="Tewari, A" sort="Tewari, A" uniqKey="Tewari A" first="A" last="Tewari">A. Tewari</name><name sortKey="Watabe, K" sort="Watabe, K" uniqKey="Watabe K" first="K" last="Watabe">K. Watabe</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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