Conserved generation of short products at piRNA loci
Identifieur interne : 000801 ( Ncbi/Checkpoint ); précédent : 000800; suivant : 000802Conserved generation of short products at piRNA loci
Auteurs : Philipp Berninger [Suisse, France] ; Lukasz Jaskiewicz [Suisse] ; Mohsen Khorshid [Suisse] ; Mihaela Zavolan [Suisse]Source :
- BMC Genomics [ 1471-2164 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Petit ARN interférent, Platypus, Rétroéléments.
- Animaux, Mâle, Rats, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Small Interfering, Retroelements.
- genetics : Platypus.
- Animals, Male, Mice, Rats.
Abstract
The piRNA pathway operates in animal germ lines to ensure genome integrity through retrotransposon silencing. The Piwi protein-associated small RNAs (piRNAs) guide Piwi proteins to retrotransposon transcripts, which are degraded and thereby post-transcriptionally silenced through a ping-pong amplification process. Cleavage of the retrotransposon transcript defines at the same time the 5' end of a secondary piRNA that will in turn guide a Piwi protein to a primary piRNA precursor, thereby amplifying primary piRNAs. Although several studies provided evidence that this mechanism is conserved among metazoa, how the process is initiated and what enzymatic activities are responsible for generating the primary and secondary piRNAs are not entirely clear.
Here we analyzed small RNAs from three mammalian species, seeking to gain further insight into the mechanisms responsible for the piRNA amplification loop. We found that in all these species piRNA-directed targeting is accompanied by the generation of short sequences that have a very precisely defined length, 19 nucleotides, and a specific spatial relationship with the guide piRNAs.
This suggests that the processing of the 5' product of piRNA-guided cleavage occurs while the piRNA target is engaged by the Piwi protein. Although they are not stabilized through methylation of their 3' ends, the 19-mers are abundant not only in testes lysates but also in immunoprecipitates of Miwi and Mili proteins. They will enable more accurate identification of piRNA loci in deep sequencing data sets.
Url:
DOI: 10.1186/1471-2164-12-46
PubMed: 21247452
PubMed Central: 3037900
Affiliations:
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PMC:3037900Le document en format XML
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The piRNA pathway operates in animal germ lines to ensure genome integrity through retrotransposon silencing. The Piwi protein-associated small RNAs (piRNAs) guide Piwi proteins to retrotransposon transcripts, which are degraded and thereby post-transcriptionally silenced through a ping-pong amplification process. Cleavage of the retrotransposon transcript defines at the same time the 5' end of a secondary piRNA that will in turn guide a Piwi protein to a primary piRNA precursor, thereby amplifying primary piRNAs. Although several studies provided evidence that this mechanism is conserved among metazoa, how the process is initiated and what enzymatic activities are responsible for generating the primary and secondary piRNAs are not entirely clear.</p>
</sec>
<sec><title>Results</title>
<p>Here we analyzed small RNAs from three mammalian species, seeking to gain further insight into the mechanisms responsible for the piRNA amplification loop. We found that in all these species piRNA-directed targeting is accompanied by the generation of short sequences that have a very precisely defined length, 19 nucleotides, and a specific spatial relationship with the guide piRNAs.</p>
</sec>
<sec><title>Conclusions</title>
<p>This suggests that the processing of the 5' product of piRNA-guided cleavage occurs while the piRNA target is engaged by the Piwi protein. Although they are not stabilized through methylation of their 3' ends, the 19-mers are abundant not only in testes lysates but also in immunoprecipitates of Miwi and Mili proteins. They will enable more accurate identification of piRNA loci in deep sequencing data sets.</p>
</sec>
</div>
</front>
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</author>
<author><name sortKey="Sigova, A" uniqKey="Sigova A">A Sigova</name>
</author>
<author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Seitz, H" uniqKey="Seitz H">H Seitz</name>
</author>
<author><name sortKey="Gvozdev, V" uniqKey="Gvozdev V">V Gvozdev</name>
</author>
<author><name sortKey="Zamore, Pd D" uniqKey="Zamore P">PD D Zamore</name>
</author>
</analytic>
</biblStruct>
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</author>
<author><name sortKey="Nishida, Km" uniqKey="Nishida K">KM Nishida</name>
</author>
<author><name sortKey="Mori, T" uniqKey="Mori T">T Mori</name>
</author>
<author><name sortKey="Kawamura, Y" uniqKey="Kawamura Y">Y Kawamura</name>
</author>
<author><name sortKey="Miyoshi, K" uniqKey="Miyoshi K">K Miyoshi</name>
</author>
<author><name sortKey="Nagami, T" uniqKey="Nagami T">T Nagami</name>
</author>
<author><name sortKey="Siomi, H" uniqKey="Siomi H">H Siomi</name>
</author>
<author><name sortKey="Siomi, Mc" uniqKey="Siomi M">MC Siomi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aravin, A" uniqKey="Aravin A">A Aravin</name>
</author>
<author><name sortKey="Gaidatzis, D" uniqKey="Gaidatzis D">D Gaidatzis</name>
</author>
<author><name sortKey="Pfeffer, S" uniqKey="Pfeffer S">S Pfeffer</name>
</author>
<author><name sortKey="Quintana, Ml" uniqKey="Quintana M">ML Quintana</name>
</author>
<author><name sortKey="Landgraf, P" uniqKey="Landgraf P">P Landgraf</name>
</author>
<author><name sortKey="Iovino, N" uniqKey="Iovino N">N Iovino</name>
</author>
<author><name sortKey="Morris, P" uniqKey="Morris P">P Morris</name>
</author>
<author><name sortKey="Brownstein, Mj" uniqKey="Brownstein M">MJ Brownstein</name>
</author>
<author><name sortKey="Miyagawa, Sk" uniqKey="Miyagawa S">SK Miyagawa</name>
</author>
<author><name sortKey="Nakano, T" uniqKey="Nakano T">T Nakano</name>
</author>
<author><name sortKey="Chien, M" uniqKey="Chien M">M Chien</name>
</author>
<author><name sortKey="Russo, Jj" uniqKey="Russo J">JJ Russo</name>
</author>
<author><name sortKey="Ju, J" uniqKey="Ju J">J Ju</name>
</author>
<author><name sortKey="Sheridan, R" uniqKey="Sheridan R">R Sheridan</name>
</author>
<author><name sortKey="Sander, C" uniqKey="Sander C">C Sander</name>
</author>
<author><name sortKey="Zavolan, M" uniqKey="Zavolan M">M Zavolan</name>
</author>
<author><name sortKey="Tuschl, T" uniqKey="Tuschl T">T Tuschl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Girard, A" uniqKey="Girard A">A Girard</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
<author><name sortKey="Carmell, Ma" uniqKey="Carmell M">MA Carmell</name>
</author>
</analytic>
</biblStruct>
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</author>
<author><name sortKey="Sakaguchi, Y" uniqKey="Sakaguchi Y">Y Sakaguchi</name>
</author>
<author><name sortKey="Suzuki, T" uniqKey="Suzuki T">T Suzuki</name>
</author>
<author><name sortKey="Suzuki, T" uniqKey="Suzuki T">T Suzuki</name>
</author>
<author><name sortKey="Siomi, H" uniqKey="Siomi H">H Siomi</name>
</author>
<author><name sortKey="Siomi, Mc" uniqKey="Siomi M">MC Siomi</name>
</author>
</analytic>
</biblStruct>
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</author>
<author><name sortKey="Stark, A" uniqKey="Stark A">A Stark</name>
</author>
<author><name sortKey="Dus, M" uniqKey="Dus M">M Dus</name>
</author>
<author><name sortKey="Kellis, M" uniqKey="Kellis M">M Kellis</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
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<biblStruct><analytic><author><name sortKey="Brennecke, J" uniqKey="Brennecke J">J Brennecke</name>
</author>
<author><name sortKey="Malone, Cd" uniqKey="Malone C">CD Malone</name>
</author>
<author><name sortKey="Aravin, Aa" uniqKey="Aravin A">AA Aravin</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Stark, A" uniqKey="Stark A">A Stark</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
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</author>
<author><name sortKey="Kamminga, Lm" uniqKey="Kamminga L">LM Kamminga</name>
</author>
<author><name sortKey="Berezikov, E" uniqKey="Berezikov E">E Berezikov</name>
</author>
<author><name sortKey="Cronembold, D" uniqKey="Cronembold D">D Cronembold</name>
</author>
<author><name sortKey="Girard, A" uniqKey="Girard A">A Girard</name>
</author>
<author><name sortKey="Van Den Elst, H" uniqKey="Van Den Elst H">H van den Elst</name>
</author>
<author><name sortKey="Filippov, Dv" uniqKey="Filippov D">DV Filippov</name>
</author>
<author><name sortKey="Blaser, H" uniqKey="Blaser H">H Blaser</name>
</author>
<author><name sortKey="Raz, E" uniqKey="Raz E">E Raz</name>
</author>
<author><name sortKey="Moens, Cb" uniqKey="Moens C">CB Moens</name>
</author>
<author><name sortKey="Plasterk, Rha" uniqKey="Plasterk R">RHA Plasterk</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
<author><name sortKey="Draper, Bw" uniqKey="Draper B">BW Draper</name>
</author>
<author><name sortKey="Ketting, Rf" uniqKey="Ketting R">RF Ketting</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Houwing, S" uniqKey="Houwing S">S Houwing</name>
</author>
<author><name sortKey="Berezikov, E" uniqKey="Berezikov E">E Berezikov</name>
</author>
<author><name sortKey="Ketting, Rf" uniqKey="Ketting R">RF Ketting</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aravin, Aa" uniqKey="Aravin A">AA Aravin</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Girard, A" uniqKey="Girard A">A Girard</name>
</author>
<author><name sortKey="Toth, Kf" uniqKey="Toth K">KF Toth</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carmell, Ma" uniqKey="Carmell M">MA Carmell</name>
</author>
<author><name sortKey="Girard, A" uniqKey="Girard A">A Girard</name>
</author>
<author><name sortKey="Van De Kant, Hjg" uniqKey="Van De Kant H">HJG van de Kant</name>
</author>
<author><name sortKey="Bourc His, D" uniqKey="Bourc His D">D Bourc'his</name>
</author>
<author><name sortKey="Bestor, Th" uniqKey="Bestor T">TH Bestor</name>
</author>
<author><name sortKey="De Rooij, Dg" uniqKey="De Rooij D">DG de Rooij </name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aravin, Aa" uniqKey="Aravin A">AA Aravin</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Bourc His, D" uniqKey="Bourc His D">D Bourc'his</name>
</author>
<author><name sortKey="Schaefer, C" uniqKey="Schaefer C">C Schaefer</name>
</author>
<author><name sortKey="Pezic, D" uniqKey="Pezic D">D Pezic</name>
</author>
<author><name sortKey="Toth, Kf" uniqKey="Toth K">KF Toth</name>
</author>
<author><name sortKey="Bestor, T" uniqKey="Bestor T">T Bestor</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miyagawa, Sk" uniqKey="Miyagawa S">SK Miyagawa</name>
</author>
<author><name sortKey="Watanabe, T" uniqKey="Watanabe T">T Watanabe</name>
</author>
<author><name sortKey="Gotoh, K" uniqKey="Gotoh K">K Gotoh</name>
</author>
<author><name sortKey="Totoki, Y" uniqKey="Totoki Y">Y Totoki</name>
</author>
<author><name sortKey="Toyoda, A" uniqKey="Toyoda A">A Toyoda</name>
</author>
<author><name sortKey="Ikawa, M" uniqKey="Ikawa M">M Ikawa</name>
</author>
<author><name sortKey="Asada, N" uniqKey="Asada N">N Asada</name>
</author>
<author><name sortKey="Kojima, K" uniqKey="Kojima K">K Kojima</name>
</author>
<author><name sortKey="Yamaguchi, Y" uniqKey="Yamaguchi Y">Y Yamaguchi</name>
</author>
<author><name sortKey="Ijiri, T" uniqKey="Ijiri T">T Ijiri</name>
</author>
<author><name sortKey="Hata, K" uniqKey="Hata K">K Hata</name>
</author>
<author><name sortKey="Li, E" uniqKey="Li E">E Li</name>
</author>
<author><name sortKey="Matsuda, Y" uniqKey="Matsuda Y">Y Matsuda</name>
</author>
<author><name sortKey="Kimura, T" uniqKey="Kimura T">T Kimura</name>
</author>
<author><name sortKey="Okabe, M" uniqKey="Okabe M">M Okabe</name>
</author>
<author><name sortKey="Sakaki, Y" uniqKey="Sakaki Y">Y Sakaki</name>
</author>
<author><name sortKey="Sasaki, H" uniqKey="Sasaki H">H Sasaki</name>
</author>
<author><name sortKey="Nakano, T" uniqKey="Nakano T">T Nakano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Graham Ruby, J" uniqKey="Graham Ruby J">J Graham Ruby</name>
</author>
<author><name sortKey="Jan, C" uniqKey="Jan C">C Jan</name>
</author>
<author><name sortKey="Player, C" uniqKey="Player C">C Player</name>
</author>
<author><name sortKey="Axtell, Mj" uniqKey="Axtell M">MJ Axtell</name>
</author>
<author><name sortKey="Lee, W" uniqKey="Lee W">W Lee</name>
</author>
<author><name sortKey="Nusbaum, C" uniqKey="Nusbaum C">C Nusbaum</name>
</author>
<author><name sortKey="Ge, H" uniqKey="Ge H">H Ge</name>
</author>
<author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, G" uniqKey="Wang G">G Wang</name>
</author>
<author><name sortKey="Reinke, V" uniqKey="Reinke V">V Reinke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Das, Pp" uniqKey="Das P">PP Das</name>
</author>
<author><name sortKey="Bagijn, Mp" uniqKey="Bagijn M">MP Bagijn</name>
</author>
<author><name sortKey="Goldstein, Ld" uniqKey="Goldstein L">LD Goldstein</name>
</author>
<author><name sortKey="Woolford, Jr" uniqKey="Woolford J">JR Woolford</name>
</author>
<author><name sortKey="Lehrbach, Nj" uniqKey="Lehrbach N">NJ Lehrbach</name>
</author>
<author><name sortKey="Sapetschnig, A" uniqKey="Sapetschnig A">A Sapetschnig</name>
</author>
<author><name sortKey="Buhecha, Hr" uniqKey="Buhecha H">HR Buhecha</name>
</author>
<author><name sortKey="Gilchrist, Mj" uniqKey="Gilchrist M">MJ Gilchrist</name>
</author>
<author><name sortKey="Howe, Kl" uniqKey="Howe K">KL Howe</name>
</author>
<author><name sortKey="Stark, R" uniqKey="Stark R">R Stark</name>
</author>
<author><name sortKey="Matthews, N" uniqKey="Matthews N">N Matthews</name>
</author>
<author><name sortKey="Berezikov, E" uniqKey="Berezikov E">E Berezikov</name>
</author>
<author><name sortKey="Ketting, Rf" uniqKey="Ketting R">RF Ketting</name>
</author>
<author><name sortKey="Tavare, S" uniqKey="Tavare S">S Tavare</name>
</author>
<author><name sortKey="Miska, Ea" uniqKey="Miska E">EA Miska</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Batista, Pj" uniqKey="Batista P">PJ Batista</name>
</author>
<author><name sortKey="Graham Ruby, J" uniqKey="Graham Ruby J">J Graham Ruby</name>
</author>
<author><name sortKey="Claycomb, Jm" uniqKey="Claycomb J">JM Claycomb</name>
</author>
<author><name sortKey="Chiang, R" uniqKey="Chiang R">R Chiang</name>
</author>
<author><name sortKey="Fahlgren, N" uniqKey="Fahlgren N">N Fahlgren</name>
</author>
<author><name sortKey="Kasschau, Kd" uniqKey="Kasschau K">KD Kasschau</name>
</author>
<author><name sortKey="Chaves, Da" uniqKey="Chaves D">DA Chaves</name>
</author>
<author><name sortKey="Gu, W" uniqKey="Gu W">W Gu</name>
</author>
<author><name sortKey="Vasale, Jj" uniqKey="Vasale J">JJ Vasale</name>
</author>
<author><name sortKey="Duan, S" uniqKey="Duan S">S Duan</name>
</author>
<author><name sortKey="Conte, D" uniqKey="Conte D">D Conte</name>
</author>
<author><name sortKey="Luo, S" uniqKey="Luo S">S Luo</name>
</author>
<author><name sortKey="Schroth, Gp" uniqKey="Schroth G">GP Schroth</name>
</author>
<author><name sortKey="Carrington, Jc" uniqKey="Carrington J">JC Carrington</name>
</author>
<author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name>
</author>
<author><name sortKey="Mello, Cc" uniqKey="Mello C">CC Mello</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gunawardane, Ls" uniqKey="Gunawardane L">LS Gunawardane</name>
</author>
<author><name sortKey="Saito, K" uniqKey="Saito K">K Saito</name>
</author>
<author><name sortKey="Nishida, Km" uniqKey="Nishida K">KM Nishida</name>
</author>
<author><name sortKey="Miyoshi, K" uniqKey="Miyoshi K">K Miyoshi</name>
</author>
<author><name sortKey="Kawamura, Y" uniqKey="Kawamura Y">Y Kawamura</name>
</author>
<author><name sortKey="Nagami, T" uniqKey="Nagami T">T Nagami</name>
</author>
<author><name sortKey="Siomi, H" uniqKey="Siomi H">H Siomi</name>
</author>
<author><name sortKey="Siomi, Mc" uniqKey="Siomi M">MC Siomi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Horwich, Md" uniqKey="Horwich M">MD Horwich</name>
</author>
<author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Matranga, C" uniqKey="Matranga C">C Matranga</name>
</author>
<author><name sortKey="Vagin, V" uniqKey="Vagin V">V Vagin</name>
</author>
<author><name sortKey="Farley, G" uniqKey="Farley G">G Farley</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Zamore, Pd" uniqKey="Zamore P">PD Zamore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kirino, Y" uniqKey="Kirino Y">Y Kirino</name>
</author>
<author><name sortKey="Mourelatos, Z" uniqKey="Mourelatos Z">Z Mourelatos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Friedlaender, Mr" uniqKey="Friedlaender M">MR Friedlaender</name>
</author>
<author><name sortKey="Adamidi, C" uniqKey="Adamidi C">C Adamidi</name>
</author>
<author><name sortKey="Han, T" uniqKey="Han T">T Han</name>
</author>
<author><name sortKey="Lebedeva, S" uniqKey="Lebedeva S">S Lebedeva</name>
</author>
<author><name sortKey="Isenbarger, Ta" uniqKey="Isenbarger T">TA Isenbarger</name>
</author>
<author><name sortKey="Hirst, M" uniqKey="Hirst M">M Hirst</name>
</author>
<author><name sortKey="Marra, M" uniqKey="Marra M">M Marra</name>
</author>
<author><name sortKey="Nusbaum, C" uniqKey="Nusbaum C">C Nusbaum</name>
</author>
<author><name sortKey="Lee, Wl" uniqKey="Lee W">WL Lee</name>
</author>
<author><name sortKey="Jenkin, Jc" uniqKey="Jenkin J">JC Jenkin</name>
</author>
<author><name sortKey="Alvarado, As" uniqKey="Alvarado A">AS Alvarado</name>
</author>
<author><name sortKey="Kim, Jk" uniqKey="Kim J">JK Kim</name>
</author>
<author><name sortKey="Rajewsky, N" uniqKey="Rajewsky N">N Rajewsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grimson, A" uniqKey="Grimson A">A Grimson</name>
</author>
<author><name sortKey="Srivastava, M" uniqKey="Srivastava M">M Srivastava</name>
</author>
<author><name sortKey="Fahey, B" uniqKey="Fahey B">B Fahey</name>
</author>
<author><name sortKey="Woodcroft, Bj" uniqKey="Woodcroft B">BJ Woodcroft</name>
</author>
<author><name sortKey="Rosaria Chiang, H" uniqKey="Rosaria Chiang H">H Rosaria Chiang</name>
</author>
<author><name sortKey="King, N" uniqKey="King N">N King</name>
</author>
<author><name sortKey="Degnan, Bm" uniqKey="Degnan B">BM Degnan</name>
</author>
<author><name sortKey="Rokhsar, Ds" uniqKey="Rokhsar D">DS Rokhsar</name>
</author>
<author><name sortKey="Bartel, Dp" uniqKey="Bartel D">DP Bartel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Betel, D" uniqKey="Betel D">D Betel</name>
</author>
<author><name sortKey="Sheridan, R" uniqKey="Sheridan R">R Sheridan</name>
</author>
<author><name sortKey="Marks, Ds" uniqKey="Marks D">DS Marks</name>
</author>
<author><name sortKey="Sander, C" uniqKey="Sander C">C Sander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Robine, N" uniqKey="Robine N">N Robine</name>
</author>
<author><name sortKey="Lau, Nc" uniqKey="Lau N">NC Lau</name>
</author>
<author><name sortKey="Balla, S" uniqKey="Balla S">S Balla</name>
</author>
<author><name sortKey="Jin, Z" uniqKey="Jin Z">Z Jin</name>
</author>
<author><name sortKey="Okamura, K" uniqKey="Okamura K">K Okamura</name>
</author>
<author><name sortKey="Miyagawa, Sk" uniqKey="Miyagawa S">SK Miyagawa</name>
</author>
<author><name sortKey="Blower, Md" uniqKey="Blower M">MD Blower</name>
</author>
<author><name sortKey="Lai, Ec" uniqKey="Lai E">EC Lai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Linsen, Sev" uniqKey="Linsen S">SEV Linsen</name>
</author>
<author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name>
</author>
<author><name sortKey="De Bruijn, E" uniqKey="De Bruijn E">E de Bruijn</name>
</author>
<author><name sortKey="Cuppen, E" uniqKey="Cuppen E">E Cuppen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murchison, Ep" uniqKey="Murchison E">EP Murchison</name>
</author>
<author><name sortKey="Kheradpour, P" uniqKey="Kheradpour P">P Kheradpour</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name>
</author>
<author><name sortKey="Hodges, E" uniqKey="Hodges E">E Hodges</name>
</author>
<author><name sortKey="Xuan, Z" uniqKey="Xuan Z">Z Xuan</name>
</author>
<author><name sortKey="Kellis, M" uniqKey="Kellis M">M Kellis</name>
</author>
<author><name sortKey="Gruetzner, F" uniqKey="Gruetzner F">F Gruetzner</name>
</author>
<author><name sortKey="Stark, A" uniqKey="Stark A">A Stark</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Malone, Cd" uniqKey="Malone C">CD Malone</name>
</author>
<author><name sortKey="Brennecke, J" uniqKey="Brennecke J">J Brennecke</name>
</author>
<author><name sortKey="Dus, M" uniqKey="Dus M">M Dus</name>
</author>
<author><name sortKey="Stark, A" uniqKey="Stark A">A Stark</name>
</author>
<author><name sortKey="Richard Mccombie, W" uniqKey="Richard Mccombie W">W Richard McCombie</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alefelder, S" uniqKey="Alefelder S">S Alefelder</name>
</author>
<author><name sortKey="Patel, Bk" uniqKey="Patel B">BK Patel</name>
</author>
<author><name sortKey="Eckstein, F" uniqKey="Eckstein F">F Eckstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Berninger, P" uniqKey="Berninger P">P Berninger</name>
</author>
<author><name sortKey="Gaidatzis, D" uniqKey="Gaidatzis D">D Gaidatzis</name>
</author>
<author><name sortKey="Van Nimwegen, E" uniqKey="Van Nimwegen E">E van Nimwegen</name>
</author>
<author><name sortKey="Zavolan, M" uniqKey="Zavolan M">M Zavolan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olsen, Aj" uniqKey="Olsen A">AJ Olsen</name>
</author>
<author><name sortKey="Brennecke, J" uniqKey="Brennecke J">J Brennecke</name>
</author>
<author><name sortKey="Aravin, Aa" uniqKey="Aravin A">AA Aravin</name>
</author>
<author><name sortKey="Hannon, Gj" uniqKey="Hannon G">GJ Hannon</name>
</author>
<author><name sortKey="Sachidanandam, R" uniqKey="Sachidanandam R">R Sachidanandam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hafner, M" uniqKey="Hafner M">M Hafner</name>
</author>
<author><name sortKey="Landgraf, P" uniqKey="Landgraf P">P Landgraf</name>
</author>
<author><name sortKey="Ludwig, J" uniqKey="Ludwig J">J Ludwig</name>
</author>
<author><name sortKey="Rice, A" uniqKey="Rice A">A Rice</name>
</author>
<author><name sortKey="Ojo, T" uniqKey="Ojo T">T Ojo</name>
</author>
<author><name sortKey="Lin, C" uniqKey="Lin C">C Lin</name>
</author>
<author><name sortKey="Holoch, D" uniqKey="Holoch D">D Holoch</name>
</author>
<author><name sortKey="Lim, C" uniqKey="Lim C">C Lim</name>
</author>
<author><name sortKey="Tuschl, T" uniqKey="Tuschl T">T Tuschl</name>
</author>
</analytic>
</biblStruct>
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