Intercalation of single-strand oligonucleotides between nucleolipid anionic membranes: a neutron diffraction study.
Identifieur interne : 000707 ( Ncbi/Checkpoint ); précédent : 000706; suivant : 000708Intercalation of single-strand oligonucleotides between nucleolipid anionic membranes: a neutron diffraction study.
Auteurs : Silvia Milani [Italie] ; Debora Berti ; Silvia Dante ; Thomas Hauss ; Piero BaglioniSource :
- Langmuir : the ACS journal of surfaces and colloids [ 0743-7463 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Single-Stranded, Liposomes, Oligodeoxyribonucleotides, Phospholipids.
- chemical : Membranes, Artificial.
- Neutron Diffraction, Volatilization.
Abstract
This contribution presents a neutron diffraction investigation of anionic lamellar phases composed of mixtures of 1-palmitoyl, 2-oleoyl phosphatidyl-nucleosides (POPN, where N is either adenosine or uridine), and POPC (1-palmitoyl,2-oleoyl-phosphatidyl-choline). Their behavior is studied for two different mole ratios and in the presence of nucleic acids. The samples are formed by the evaporation of liposomal dispersions prepared in water or in solutions containing single-strand oligonucleotides. Previous small angle X-ray scattering (SAXS) experiments on the system POPA/polyU (polyuridylic acid, high degree of polymerization, synthetic ribonucleic acid) proved that the insertion and ordering of the biopolymer in the phospholipid lamellae were driven by molecular recognition. In the present study, we extend the previous investigation to single-strand monodisperse oligonucleotides (50-mers). Structural details of the membranes were obtained from the analysis of the neutron diffraction scattering length density profiles. The evidence of direct and specific interactions, driven by molecular recognition between the nucleic polar heads of the nucleolipid and the single-strand nucleic acid, is strengthened by the comparison with identically charged bilayers formed by POPG/POPC. These results contribute to the understanding of the parameters governing the interactions between nucleolipid membranes and oligonucleotides, providing a novel strategy for the design of lipid-based vehicles for nucleic acids.
DOI: 10.1021/la8029825
PubMed: 19714893
Affiliations:
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last="Dante">Silvia Dante</name></author><author><name sortKey="Hauss, Thomas" sort="Hauss, Thomas" uniqKey="Hauss T" first="Thomas" last="Hauss">Thomas Hauss</name></author><author><name sortKey="Baglioni, Piero" sort="Baglioni, Piero" uniqKey="Baglioni P" first="Piero" last="Baglioni">Piero Baglioni</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19714893</idno><idno type="pmid">19714893</idno><idno type="doi">10.1021/la8029825</idno><idno type="wicri:Area/PubMed/Corpus">001F97</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F97</idno><idno type="wicri:Area/PubMed/Curation">001F97</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F97</idno><idno type="wicri:Area/PubMed/Checkpoint">001F04</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F04</idno><idno 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first="Debora" last="Berti">Debora Berti</name></author><author><name sortKey="Dante, Silvia" sort="Dante, Silvia" uniqKey="Dante S" first="Silvia" last="Dante">Silvia Dante</name></author><author><name sortKey="Hauss, Thomas" sort="Hauss, Thomas" uniqKey="Hauss T" first="Thomas" last="Hauss">Thomas Hauss</name></author><author><name sortKey="Baglioni, Piero" sort="Baglioni, Piero" uniqKey="Baglioni P" first="Piero" last="Baglioni">Piero Baglioni</name></author></analytic><series><title level="j">Langmuir : the ACS journal of surfaces and colloids</title><idno type="ISSN">0743-7463</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Single-Stranded (chemistry)</term><term>Liposomes (chemistry)</term><term>Membranes, Artificial</term><term>Neutron Diffraction</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Phospholipids 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artificielle</term><term>Oligodésoxyribonucléotides</term><term>Phospholipides</term><term>Volatilisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This contribution presents a neutron diffraction investigation of anionic lamellar phases composed of mixtures of 1-palmitoyl, 2-oleoyl phosphatidyl-nucleosides (POPN, where N is either adenosine or uridine), and POPC (1-palmitoyl,2-oleoyl-phosphatidyl-choline). Their behavior is studied for two different mole ratios and in the presence of nucleic acids. The samples are formed by the evaporation of liposomal dispersions prepared in water or in solutions containing single-strand oligonucleotides. Previous small angle X-ray scattering (SAXS) experiments on the system POPA/polyU (polyuridylic acid, high degree of polymerization, synthetic ribonucleic acid) proved that the insertion and ordering of the biopolymer in the phospholipid lamellae were driven by molecular recognition. In the present study, we extend the previous investigation to single-strand monodisperse oligonucleotides (50-mers). Structural details of the membranes were obtained from the analysis of the neutron diffraction scattering length density profiles. The evidence of direct and specific interactions, driven by molecular recognition between the nucleic polar heads of the nucleolipid and the single-strand nucleic acid, is strengthened by the comparison with identically charged bilayers formed by POPG/POPC. These results contribute to the understanding of the parameters governing the interactions between nucleolipid membranes and oligonucleotides, providing a novel strategy for the design of lipid-based vehicles for nucleic acids.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Baglioni, Piero" sort="Baglioni, Piero" uniqKey="Baglioni P" first="Piero" last="Baglioni">Piero Baglioni</name><name sortKey="Berti, Debora" sort="Berti, Debora" uniqKey="Berti D" first="Debora" last="Berti">Debora Berti</name><name sortKey="Dante, Silvia" sort="Dante, Silvia" uniqKey="Dante S" first="Silvia" last="Dante">Silvia Dante</name><name sortKey="Hauss, Thomas" sort="Hauss, Thomas" uniqKey="Hauss T" first="Thomas" last="Hauss">Thomas Hauss</name></noCountry><country name="Italie"><noRegion><name sortKey="Milani, Silvia" sort="Milani, Silvia" uniqKey="Milani S" first="Silvia" last="Milani">Silvia Milani</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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