Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B.
Identifieur interne : 000630 ( Ncbi/Checkpoint ); précédent : 000629; suivant : 000631Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B.
Auteurs : Aziz Alami Chentoufi [États-Unis] ; Nicholas R. Binder ; Noureddine Berka ; Guillaume Durand ; Alex Nguyen ; Ilham Bettahi ; Bernard Maillère ; Lbachir BenmohamedSource :
- Journal of virology [ 1098-5514 ] ; 2008.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Antigènes HLA-DR (métabolisme), Déterminants antigéniques des lymphocytes T, Femelle, Herpès (immunologie), Humains, Interféron gamma (biosynthèse), Lymphocytes T CD4+ (immunologie), Mâle, Protéines de l'enveloppe virale (immunologie), Épitopes immunodominants.
- MESH :
- biosynthèse : Interféron gamma.
- immunologie : Herpès, Lymphocytes T CD4+, Protéines de l'enveloppe virale.
- métabolisme : Antigènes HLA-DR.
- Adolescent, Adulte, Adulte d'âge moyen, Déterminants antigéniques des lymphocytes T, Femelle, Humains, Mâle, Épitopes immunodominants.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Interferon-gamma.
- chemical , immunology : Viral Envelope Proteins.
- chemical , metabolism : HLA-DR Antigens.
- chemical : Epitopes, T-Lymphocyte, Immunodominant Epitopes.
- immunology : CD4-Positive T-Lymphocytes, Herpes Simplex.
- Adolescent, Adult, Female, Humans, Male, Middle Aged.
Abstract
The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, a library of 179 potential peptide epitopes (15-mers overlapping by 10 amino acids) was identified from HSV type 1 (HSV-1) glycoprotein B (gB), an antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) of 10 adjacent peptides each were first screened for T-cell antigenicity in 38 HSV-1-seropositive but HSV-2-seronegative individuals. Individual peptides within the two immunodominant groups (i.e., G4 and G14) were further screened with T cells from HLA-DR-genotyped and clinically defined symptomatic (n = 10) and asymptomatic (n = 10) HSV-1-seropositive healthy individuals. Peptides gB(161-175) and gB(166-180) within G4 and gB(661-675) within G14 recalled the strongest HLA-DR-dependent CD4(+) T-cell proliferation and gamma interferon production. gB(166-180), gB(661-675), and gB(666-680) elicited ex vivo CD4(+) cytotoxic T cells (CTLs) that lysed autologous HSV-1- and vaccinia virus (expressing gB)-infected lymphoblastoid cell lines. Interestingly, gB(166-180) and gB(666-680) peptide epitopes were strongly recognized by CD4(+) T cells from 10 of 10 asymptomatic patients but not by CD4(+) T cells from 10 of 10 symptomatic patients (P < 0.0001; analysis of variance posttest). Inversely, CD4(+) T cells from symptomatic patients preferentially recognized gB(661-675) (P < 0.0001). Thus, we identified three previously unrecognized CD4(+) CTL peptide epitopes in HSV-1 gB. Among these, gB(166-180) and gB(666-680) appear to be "asymptomatic" peptide epitopes and therefore should be considered in the design of future herpes vaccines.
DOI: 10.1128/JVI.00692-08
PubMed: 18799581
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002079
- to stream PubMed, to step Curation: 002079
- to stream PubMed, to step Checkpoint: 002012
- to stream Ncbi, to step Merge: 000630
- to stream Ncbi, to step Curation: 000630
Links to Exploration step
pubmed:18799581Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B.</title>
<author><name sortKey="Chentoufi, Aziz Alami" sort="Chentoufi, Aziz Alami" uniqKey="Chentoufi A" first="Aziz Alami" last="Chentoufi">Aziz Alami Chentoufi</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine, Irvine, California 92697-4375, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine, Irvine, California 92697-4375</wicri:regionArea>
<wicri:noRegion>California 92697-4375</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Binder, Nicholas R" sort="Binder, Nicholas R" uniqKey="Binder N" first="Nicholas R" last="Binder">Nicholas R. Binder</name>
</author>
<author><name sortKey="Berka, Noureddine" sort="Berka, Noureddine" uniqKey="Berka N" first="Noureddine" last="Berka">Noureddine Berka</name>
</author>
<author><name sortKey="Durand, Guillaume" sort="Durand, Guillaume" uniqKey="Durand G" first="Guillaume" last="Durand">Guillaume Durand</name>
</author>
<author><name sortKey="Nguyen, Alex" sort="Nguyen, Alex" uniqKey="Nguyen A" first="Alex" last="Nguyen">Alex Nguyen</name>
</author>
<author><name sortKey="Bettahi, Ilham" sort="Bettahi, Ilham" uniqKey="Bettahi I" first="Ilham" last="Bettahi">Ilham Bettahi</name>
</author>
<author><name sortKey="Maillere, Bernard" sort="Maillere, Bernard" uniqKey="Maillere B" first="Bernard" last="Maillère">Bernard Maillère</name>
</author>
<author><name sortKey="Benmohamed, Lbachir" sort="Benmohamed, Lbachir" uniqKey="Benmohamed L" first="Lbachir" last="Benmohamed">Lbachir Benmohamed</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2008">2008</date>
<idno type="RBID">pubmed:18799581</idno>
<idno type="pmid">18799581</idno>
<idno type="doi">10.1128/JVI.00692-08</idno>
<idno type="wicri:Area/PubMed/Corpus">002079</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002079</idno>
<idno type="wicri:Area/PubMed/Curation">002079</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002079</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002012</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002012</idno>
<idno type="wicri:Area/Ncbi/Merge">000630</idno>
<idno type="wicri:Area/Ncbi/Curation">000630</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000630</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B.</title>
<author><name sortKey="Chentoufi, Aziz Alami" sort="Chentoufi, Aziz Alami" uniqKey="Chentoufi A" first="Aziz Alami" last="Chentoufi">Aziz Alami Chentoufi</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine, Irvine, California 92697-4375, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Cellular and Molecular Immunology, The Gavin S. Herbert Eye Institute, University of California Irvine, School of Medicine, Irvine, California 92697-4375</wicri:regionArea>
<wicri:noRegion>California 92697-4375</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Binder, Nicholas R" sort="Binder, Nicholas R" uniqKey="Binder N" first="Nicholas R" last="Binder">Nicholas R. Binder</name>
</author>
<author><name sortKey="Berka, Noureddine" sort="Berka, Noureddine" uniqKey="Berka N" first="Noureddine" last="Berka">Noureddine Berka</name>
</author>
<author><name sortKey="Durand, Guillaume" sort="Durand, Guillaume" uniqKey="Durand G" first="Guillaume" last="Durand">Guillaume Durand</name>
</author>
<author><name sortKey="Nguyen, Alex" sort="Nguyen, Alex" uniqKey="Nguyen A" first="Alex" last="Nguyen">Alex Nguyen</name>
</author>
<author><name sortKey="Bettahi, Ilham" sort="Bettahi, Ilham" uniqKey="Bettahi I" first="Ilham" last="Bettahi">Ilham Bettahi</name>
</author>
<author><name sortKey="Maillere, Bernard" sort="Maillere, Bernard" uniqKey="Maillere B" first="Bernard" last="Maillère">Bernard Maillère</name>
</author>
<author><name sortKey="Benmohamed, Lbachir" sort="Benmohamed, Lbachir" uniqKey="Benmohamed L" first="Lbachir" last="Benmohamed">Lbachir Benmohamed</name>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2008" type="published">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>CD4-Positive T-Lymphocytes (immunology)</term>
<term>Epitopes, T-Lymphocyte</term>
<term>Female</term>
<term>HLA-DR Antigens (metabolism)</term>
<term>Herpes Simplex (immunology)</term>
<term>Humans</term>
<term>Immunodominant Epitopes</term>
<term>Interferon-gamma (biosynthesis)</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Antigènes HLA-DR (métabolisme)</term>
<term>Déterminants antigéniques des lymphocytes T</term>
<term>Femelle</term>
<term>Herpès (immunologie)</term>
<term>Humains</term>
<term>Interféron gamma (biosynthèse)</term>
<term>Lymphocytes T CD4+ (immunologie)</term>
<term>Mâle</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Épitopes immunodominants</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>HLA-DR Antigens</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Epitopes, T-Lymphocyte</term>
<term>Immunodominant Epitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Herpès</term>
<term>Lymphocytes T CD4+</term>
<term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term>
<term>Herpes Simplex</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes HLA-DR</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Déterminants antigéniques des lymphocytes T</term>
<term>Femelle</term>
<term>Humains</term>
<term>Mâle</term>
<term>Épitopes immunodominants</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, a library of 179 potential peptide epitopes (15-mers overlapping by 10 amino acids) was identified from HSV type 1 (HSV-1) glycoprotein B (gB), an antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) of 10 adjacent peptides each were first screened for T-cell antigenicity in 38 HSV-1-seropositive but HSV-2-seronegative individuals. Individual peptides within the two immunodominant groups (i.e., G4 and G14) were further screened with T cells from HLA-DR-genotyped and clinically defined symptomatic (n = 10) and asymptomatic (n = 10) HSV-1-seropositive healthy individuals. Peptides gB(161-175) and gB(166-180) within G4 and gB(661-675) within G14 recalled the strongest HLA-DR-dependent CD4(+) T-cell proliferation and gamma interferon production. gB(166-180), gB(661-675), and gB(666-680) elicited ex vivo CD4(+) cytotoxic T cells (CTLs) that lysed autologous HSV-1- and vaccinia virus (expressing gB)-infected lymphoblastoid cell lines. Interestingly, gB(166-180) and gB(666-680) peptide epitopes were strongly recognized by CD4(+) T cells from 10 of 10 asymptomatic patients but not by CD4(+) T cells from 10 of 10 symptomatic patients (P < 0.0001; analysis of variance posttest). Inversely, CD4(+) T cells from symptomatic patients preferentially recognized gB(661-675) (P < 0.0001). Thus, we identified three previously unrecognized CD4(+) CTL peptide epitopes in HSV-1 gB. Among these, gB(166-180) and gB(666-680) appear to be "asymptomatic" peptide epitopes and therefore should be considered in the design of future herpes vaccines.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Benmohamed, Lbachir" sort="Benmohamed, Lbachir" uniqKey="Benmohamed L" first="Lbachir" last="Benmohamed">Lbachir Benmohamed</name>
<name sortKey="Berka, Noureddine" sort="Berka, Noureddine" uniqKey="Berka N" first="Noureddine" last="Berka">Noureddine Berka</name>
<name sortKey="Bettahi, Ilham" sort="Bettahi, Ilham" uniqKey="Bettahi I" first="Ilham" last="Bettahi">Ilham Bettahi</name>
<name sortKey="Binder, Nicholas R" sort="Binder, Nicholas R" uniqKey="Binder N" first="Nicholas R" last="Binder">Nicholas R. Binder</name>
<name sortKey="Durand, Guillaume" sort="Durand, Guillaume" uniqKey="Durand G" first="Guillaume" last="Durand">Guillaume Durand</name>
<name sortKey="Maillere, Bernard" sort="Maillere, Bernard" uniqKey="Maillere B" first="Bernard" last="Maillère">Bernard Maillère</name>
<name sortKey="Nguyen, Alex" sort="Nguyen, Alex" uniqKey="Nguyen A" first="Alex" last="Nguyen">Alex Nguyen</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Chentoufi, Aziz Alami" sort="Chentoufi, Aziz Alami" uniqKey="Chentoufi A" first="Aziz Alami" last="Chentoufi">Aziz Alami Chentoufi</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000630 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000630 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:18799581 |texte= Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:18799581" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |