Properties of a concentrated minipool solvent-detergent treated cryoprecipitate processed in single-use bag systems.
Identifieur interne : 000610 ( Ncbi/Checkpoint ); précédent : 000609; suivant : 000611Properties of a concentrated minipool solvent-detergent treated cryoprecipitate processed in single-use bag systems.
Auteurs : T. Burnouf [France] ; C. Caron ; M. Radosevich ; H A Goubran ; J. Goudemand ; M. El-EkiabySource :
- Haemophilia : the official journal of the World Federation of Hemophilia [ 1365-2516 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Blood Coagulation Factors, Factor VIII, Fibrinogen, von Willebrand Factor.
- methods : Blood Preservation, Cryopreservation.
- Blood Banks, Detergents, Humans, Solvents, Virus Inactivation.
Abstract
Cryoprecipitate is still used to treat factor VIII (FVIII), von Willebrand factor (VWF) and/or fibrinogen deficiency. Recently a solvent-detergent (S/D) process of minipools of cryoprecipitate performed in a closed bag system has been designed to improve its viral safety. Still, cryoprecipitate has other drawbacks, including low concentration in active proteins, and presence of haemolytic isoagglutinins. We report here the biochemical evaluation of S/D-treated minipools of cryoprecipitates depleted of cryo-poor plasma. Cryoprecipitates were solubilized by 8 mL of a sterile glucose/saline solution, pooled in batches of 40 donations and subjected to S/D treatment in a plastic bag system using either 2% TnBP or 1% TnBP-1%Triton X-45, followed by oil extractions (n = 10). Mean (+/-SD) FVIII and fibrinogen content was 8.86 (+/-1.29) IU mL(-1) and 16.02 (+/-1.98) mg mL(-1), and 8.92 (+/-1.05) IU mL(-1) in cryoprecipitate minipools treated with 2% TnBP, and 17.26 (+/-1.71) mg mL(-1), in those treated by TnBP-Triton X-45, respectively. The WWF antigen, ristocetin cofactor and collagen binding activities were close to 10, 7 and 8 IU mL(-1), respectively, and were not affected by either SD treatment. VWF multimeric pattern of SD-treated cryoprecipitates were similar to that of normal plasma, and the >15 mers and >10 mers content was identical to that of the starting cryoprecipitates. The anti-A and anti-B titre was 0-1 and 0-1/8, respectively. Therefore, it is possible to prepare virally inactivated cryoprecipitate minipools depleted of isoagglutinins and enriched in functional FVIII, VWF and clottable fibrinogen.
DOI: 10.1111/j.1365-2516.2008.01797.x
PubMed: 18565126
Affiliations:
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Caron</name></author><author><name sortKey="Radosevich, M" sort="Radosevich, M" uniqKey="Radosevich M" first="M" last="Radosevich">M. Radosevich</name></author><author><name sortKey="Goubran, H A" sort="Goubran, H A" uniqKey="Goubran H" first="H A" last="Goubran">H A Goubran</name></author><author><name sortKey="Goudemand, J" sort="Goudemand, J" uniqKey="Goudemand J" first="J" last="Goudemand">J. Goudemand</name></author><author><name sortKey="El Ekiaby, M" sort="El Ekiaby, M" uniqKey="El Ekiaby M" first="M" last="El-Ekiaby">M. 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Burnouf</name><affiliation wicri:level="3"><nlm:affiliation>Human Plasma Product Services, Research Department, Lille, France. tburnou@attglobal.net</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Human Plasma Product Services, Research Department, Lille</wicri:regionArea><placeName><region type="region">Hauts-de-France</region><region type="old region">Nord-Pas-de-Calais</region><settlement type="city">Lille</settlement></placeName></affiliation></author><author><name sortKey="Caron, C" sort="Caron, C" uniqKey="Caron C" first="C" last="Caron">C. Caron</name></author><author><name sortKey="Radosevich, M" sort="Radosevich, M" uniqKey="Radosevich M" first="M" last="Radosevich">M. Radosevich</name></author><author><name sortKey="Goubran, H A" sort="Goubran, H A" uniqKey="Goubran H" first="H A" last="Goubran">H A Goubran</name></author><author><name sortKey="Goudemand, J" sort="Goudemand, J" uniqKey="Goudemand J" first="J" last="Goudemand">J. Goudemand</name></author><author><name sortKey="El Ekiaby, M" sort="El Ekiaby, M" uniqKey="El Ekiaby M" first="M" last="El-Ekiaby">M. El-Ekiaby</name></author></analytic><series><title level="j">Haemophilia : the official journal of the World Federation of Hemophilia</title><idno type="eISSN">1365-2516</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood Banks</term><term>Blood Coagulation Factors (analysis)</term><term>Blood Preservation (methods)</term><term>Cryopreservation (methods)</term><term>Detergents</term><term>Factor VIII (analysis)</term><term>Fibrinogen (analysis)</term><term>Humans</term><term>Solvents</term><term>Virus Inactivation</term><term>von Willebrand Factor (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Banques de sang</term><term>Conservation de sang ()</term><term>Cryoconservation ()</term><term>Détergents</term><term>Facteur VIII (analyse)</term><term>Facteur de von Willebrand (analyse)</term><term>Facteurs de la coagulation sanguine (analyse)</term><term>Fibrinogène (analyse)</term><term>Humains</term><term>Inactivation virale</term><term>Solvants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Blood Coagulation Factors</term><term>Factor VIII</term><term>Fibrinogen</term><term>von Willebrand Factor</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Facteur VIII</term><term>Facteur de von Willebrand</term><term>Facteurs de la coagulation sanguine</term><term>Fibrinogène</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blood Preservation</term><term>Cryopreservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blood Banks</term><term>Detergents</term><term>Humans</term><term>Solvents</term><term>Virus Inactivation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banques de sang</term><term>Conservation de sang</term><term>Cryoconservation</term><term>Détergents</term><term>Humains</term><term>Inactivation virale</term><term>Solvants</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cryoprecipitate is still used to treat factor VIII (FVIII), von Willebrand factor (VWF) and/or fibrinogen deficiency. Recently a solvent-detergent (S/D) process of minipools of cryoprecipitate performed in a closed bag system has been designed to improve its viral safety. Still, cryoprecipitate has other drawbacks, including low concentration in active proteins, and presence of haemolytic isoagglutinins. We report here the biochemical evaluation of S/D-treated minipools of cryoprecipitates depleted of cryo-poor plasma. Cryoprecipitates were solubilized by 8 mL of a sterile glucose/saline solution, pooled in batches of 40 donations and subjected to S/D treatment in a plastic bag system using either 2% TnBP or 1% TnBP-1%Triton X-45, followed by oil extractions (n = 10). Mean (+/-SD) FVIII and fibrinogen content was 8.86 (+/-1.29) IU mL(-1) and 16.02 (+/-1.98) mg mL(-1), and 8.92 (+/-1.05) IU mL(-1) in cryoprecipitate minipools treated with 2% TnBP, and 17.26 (+/-1.71) mg mL(-1), in those treated by TnBP-Triton X-45, respectively. The WWF antigen, ristocetin cofactor and collagen binding activities were close to 10, 7 and 8 IU mL(-1), respectively, and were not affected by either SD treatment. VWF multimeric pattern of SD-treated cryoprecipitates were similar to that of normal plasma, and the >15 mers and >10 mers content was identical to that of the starting cryoprecipitates. The anti-A and anti-B titre was 0-1 and 0-1/8, respectively. Therefore, it is possible to prepare virally inactivated cryoprecipitate minipools depleted of isoagglutinins and enriched in functional FVIII, VWF and clottable fibrinogen.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li></region><settlement><li>Lille</li></settlement></list><tree><noCountry><name sortKey="Caron, C" sort="Caron, C" uniqKey="Caron C" first="C" last="Caron">C. Caron</name><name sortKey="El Ekiaby, M" sort="El Ekiaby, M" uniqKey="El Ekiaby M" first="M" last="El-Ekiaby">M. El-Ekiaby</name><name sortKey="Goubran, H A" sort="Goubran, H A" uniqKey="Goubran H" first="H A" last="Goubran">H A Goubran</name><name sortKey="Goudemand, J" sort="Goudemand, J" uniqKey="Goudemand J" first="J" last="Goudemand">J. Goudemand</name><name sortKey="Radosevich, M" sort="Radosevich, M" uniqKey="Radosevich M" first="M" last="Radosevich">M. Radosevich</name></noCountry><country name="France"><region name="Hauts-de-France"><name sortKey="Burnouf, T" sort="Burnouf, T" uniqKey="Burnouf T" first="T" last="Burnouf">T. Burnouf</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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